Rotavirus A pheasant-tc/GER/10V0112H5/2010/G23P[37]

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A; Rotavirus A isolates

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0CSN4|L0CSN4_9REOV Non-structural protein 5 OS=Rotavirus A pheasant-tc/GER/10V0112H5/2010/G23P[37] OX=1271534 PE=4 SV=1
MM1 pKa = 7.49YY2 pKa = 9.41STEE5 pKa = 4.05CTSFFLEE12 pKa = 4.15IIFYY16 pKa = 10.44VILYY20 pKa = 7.18TIISSRR26 pKa = 11.84LSRR29 pKa = 11.84MSRR32 pKa = 11.84LFYY35 pKa = 10.16WIIIFIIFSSLCVSKK50 pKa = 10.92CSSQNFGINVPITGSLDD67 pKa = 3.41VTVLNSTQTQIGLTSTLCIYY87 pKa = 10.0YY88 pKa = 9.69PKK90 pKa = 10.12EE91 pKa = 3.83ASDD94 pKa = 4.14EE95 pKa = 4.34IKK97 pKa = 10.83DD98 pKa = 4.09SEE100 pKa = 4.32WKK102 pKa = 8.72NTVSQLLLTKK112 pKa = 9.96GWPTTSVYY120 pKa = 10.89LNEE123 pKa = 4.26YY124 pKa = 9.77NDD126 pKa = 4.06LAAFSNDD133 pKa = 2.55PKK135 pKa = 10.92LYY137 pKa = 9.75CDD139 pKa = 3.72YY140 pKa = 11.28NIVLVKK146 pKa = 9.17YY147 pKa = 8.33TGSNVALDD155 pKa = 3.47ISEE158 pKa = 4.28LAEE161 pKa = 3.99LLLYY165 pKa = 10.18EE166 pKa = 4.42WLCNPMDD173 pKa = 3.53VTLYY177 pKa = 10.62YY178 pKa = 9.99YY179 pKa = 10.53QQTSEE184 pKa = 4.23SNKK187 pKa = 8.2WIAMGTDD194 pKa = 3.77CTIKK198 pKa = 10.33VCPLNVQTLGIGCQTTDD215 pKa = 2.68VNTFEE220 pKa = 4.48QVTASEE226 pKa = 4.0QLAIIDD232 pKa = 3.76VVDD235 pKa = 4.36GVNHH239 pKa = 7.27KK240 pKa = 10.36INYY243 pKa = 7.41TLTTCTIKK251 pKa = 10.89NCIRR255 pKa = 11.84LNPRR259 pKa = 11.84GNVAIIQIGGPEE271 pKa = 3.96ILDD274 pKa = 3.32ISEE277 pKa = 4.74DD278 pKa = 3.66PMVVPKK284 pKa = 9.3MVRR287 pKa = 11.84MTRR290 pKa = 11.84INWKK294 pKa = 9.41KK295 pKa = 7.27WWQVFYY301 pKa = 10.64TIVDD305 pKa = 3.85YY306 pKa = 11.35INTIVNAMSKK316 pKa = 10.25RR317 pKa = 11.84ATSLDD322 pKa = 2.99ASSYY326 pKa = 7.88YY327 pKa = 10.84LRR329 pKa = 11.84VV330 pKa = 3.27

Molecular weight: 37.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0CRY4|L0CRY4_9REOV Intermediate capsid protein VP6 OS=Rotavirus A pheasant-tc/GER/10V0112H5/2010/G23P[37] OX=1271534 PE=3 SV=1
MM1 pKa = 6.85NHH3 pKa = 5.71LVKK6 pKa = 10.67RR7 pKa = 11.84QVLQGNLLVGVSSMYY22 pKa = 10.41RR23 pKa = 11.84CPKK26 pKa = 9.39KK27 pKa = 10.15HH28 pKa = 6.4LANTCLIGIQKK39 pKa = 8.46TLDD42 pKa = 3.36HH43 pKa = 7.23LIQHH47 pKa = 6.18QMTCPPNLLLNQMQLNQMLMLACRR71 pKa = 11.84WMLDD75 pKa = 3.03QWIVRR80 pKa = 11.84QEE82 pKa = 3.96TMQKK86 pKa = 8.57MKK88 pKa = 10.79SIFHH92 pKa = 5.94SLKK95 pKa = 10.27EE96 pKa = 3.95

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5986

96

1089

498.8

57.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.396 ± 0.352

1.286 ± 0.368

6.215 ± 0.231

5.379 ± 0.269

4.227 ± 0.199

3.375 ± 0.343

1.57 ± 0.356

7.685 ± 0.271

6.799 ± 0.525

9.422 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.74 ± 0.318

6.866 ± 0.339

3.291 ± 0.316

4.059 ± 0.531

4.744 ± 0.319

7.785 ± 0.779

6.498 ± 0.388

6.365 ± 0.223

1.169 ± 0.172

5.129 ± 0.609
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski