Alfalfa dwarf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus; Alfalfa dwarf cytorhabdovirus

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6QEW5|A0A0F6QEW5_9RHAB GDP polyribonucleotidyltransferase OS=Alfalfa dwarf virus OX=998864 PE=4 SV=1
MM1 pKa = 7.7AAPNFEE7 pKa = 4.75DD8 pKa = 4.15LSNPILDD15 pKa = 3.65MAMSGLNNSGDD26 pKa = 4.05EE27 pKa = 4.38DD28 pKa = 4.16SQIDD32 pKa = 3.64PAEE35 pKa = 4.34INDD38 pKa = 3.91DD39 pKa = 3.59VEE41 pKa = 4.52MGVEE45 pKa = 4.11NPKK48 pKa = 9.54MDD50 pKa = 3.63EE51 pKa = 4.18VAATDD56 pKa = 3.47AKK58 pKa = 10.98QPVDD62 pKa = 3.67EE63 pKa = 5.32YY64 pKa = 11.17EE65 pKa = 4.58IEE67 pKa = 5.47DD68 pKa = 4.09DD69 pKa = 4.87LAYY72 pKa = 10.78DD73 pKa = 3.93PADD76 pKa = 3.32VSHH79 pKa = 6.91ALEE82 pKa = 4.56DD83 pKa = 4.01LKK85 pKa = 11.16HH86 pKa = 6.04LCDD89 pKa = 3.73VRR91 pKa = 11.84GVNYY95 pKa = 7.52TVPMEE100 pKa = 4.16NQVSKK105 pKa = 11.01LFKK108 pKa = 10.93EE109 pKa = 4.61EE110 pKa = 3.84ILCYY114 pKa = 10.47SHH116 pKa = 6.87LKK118 pKa = 9.16WYY120 pKa = 9.89IRR122 pKa = 11.84GIILANQTQVLPTVTGAIADD142 pKa = 3.86MKK144 pKa = 10.6MEE146 pKa = 4.22TQHH149 pKa = 6.19LQKK152 pKa = 10.71SSNKK156 pKa = 8.73INKK159 pKa = 8.41EE160 pKa = 3.52VSRR163 pKa = 11.84PEE165 pKa = 3.67KK166 pKa = 9.39LTRR169 pKa = 11.84EE170 pKa = 3.97ITSEE174 pKa = 3.87LRR176 pKa = 11.84SIKK179 pKa = 10.58ADD181 pKa = 3.03IQEE184 pKa = 4.19SFRR187 pKa = 11.84ASMKK191 pKa = 10.7LFMDD195 pKa = 3.92EE196 pKa = 4.29TTTNSQRR203 pKa = 11.84LQPPVKK209 pKa = 9.94EE210 pKa = 4.24EE211 pKa = 3.96KK212 pKa = 9.63VTTNLISALVKK223 pKa = 10.6SKK225 pKa = 10.97EE226 pKa = 3.87KK227 pKa = 11.22AEE229 pKa = 4.33DD230 pKa = 3.67YY231 pKa = 10.75KK232 pKa = 11.62DD233 pKa = 3.37EE234 pKa = 4.32ATVSGVKK241 pKa = 8.89TAINIPKK248 pKa = 9.72PEE250 pKa = 4.17PSKK253 pKa = 10.71KK254 pKa = 10.56GRR256 pKa = 11.84DD257 pKa = 3.3GYY259 pKa = 9.64MAEE262 pKa = 4.69KK263 pKa = 8.56RR264 pKa = 11.84AHH266 pKa = 6.07MIRR269 pKa = 11.84YY270 pKa = 8.82GLDD273 pKa = 2.85ARR275 pKa = 11.84FVKK278 pKa = 9.62EE279 pKa = 4.17QPDD282 pKa = 3.86SVIDD286 pKa = 4.4LMYY289 pKa = 10.56PDD291 pKa = 5.31DD292 pKa = 4.01AHH294 pKa = 7.56SKK296 pKa = 9.34LKK298 pKa = 10.27QMKK301 pKa = 10.08LNNQQKK307 pKa = 9.45VEE309 pKa = 4.43GYY311 pKa = 10.3

Molecular weight: 35.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6QEW1|A0A0F6QEW1_9RHAB Phosphoprotein OS=Alfalfa dwarf virus OX=998864 PE=4 SV=1
MM1 pKa = 7.98PIRR4 pKa = 11.84VGSTSYY10 pKa = 10.19TLDD13 pKa = 3.32KK14 pKa = 10.5EE15 pKa = 4.47LVVGAEE21 pKa = 3.97NGEE24 pKa = 4.24VPLTKK29 pKa = 9.94TLSLFQRR36 pKa = 11.84ATMLNAKK43 pKa = 9.7NYY45 pKa = 9.96RR46 pKa = 11.84IKK48 pKa = 10.47QLRR51 pKa = 11.84FVYY54 pKa = 10.19KK55 pKa = 10.16PRR57 pKa = 11.84TGSLGEE63 pKa = 4.03GTIEE67 pKa = 4.65TRR69 pKa = 11.84VKK71 pKa = 10.8DD72 pKa = 3.48NRR74 pKa = 11.84IDD76 pKa = 3.64SRR78 pKa = 11.84ISDD81 pKa = 4.42PIINRR86 pKa = 11.84FRR88 pKa = 11.84FDD90 pKa = 3.23ASASADD96 pKa = 3.98LKK98 pKa = 10.33WEE100 pKa = 4.03SSFWIPKK107 pKa = 10.14DD108 pKa = 3.57DD109 pKa = 4.17FKK111 pKa = 11.63SGTKK115 pKa = 10.34APIILEE121 pKa = 4.13TDD123 pKa = 3.39VLEE126 pKa = 4.95CNLQEE131 pKa = 4.78GYY133 pKa = 10.68SIGLLSIIVSVSASDD148 pKa = 3.29TMDD151 pKa = 2.94NFIYY155 pKa = 10.23RR156 pKa = 11.84RR157 pKa = 11.84PSAEE161 pKa = 3.44IRR163 pKa = 11.84EE164 pKa = 4.35DD165 pKa = 3.33PFKK168 pKa = 10.81ILAVGGSKK176 pKa = 10.07SAQSKK181 pKa = 9.27FIQTPVQRR189 pKa = 11.84MITDD193 pKa = 3.67EE194 pKa = 4.07QTGAPEE200 pKa = 3.9DD201 pKa = 4.41AEE203 pKa = 4.55MKK205 pKa = 10.0NHH207 pKa = 6.03LRR209 pKa = 11.84VIARR213 pKa = 11.84DD214 pKa = 3.81SVGSRR219 pKa = 11.84SSRR222 pKa = 11.84MIEE225 pKa = 3.65PARR228 pKa = 11.84PSGYY232 pKa = 7.45RR233 pKa = 11.84QRR235 pKa = 11.84RR236 pKa = 11.84IRR238 pKa = 11.84EE239 pKa = 3.93VV240 pKa = 2.91

Molecular weight: 27.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3937

65

2085

562.4

63.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.918 ± 0.94

1.88 ± 0.332

6.655 ± 0.383

5.867 ± 0.435

2.87 ± 0.246

5.994 ± 0.357

2.032 ± 0.276

6.528 ± 0.396

6.883 ± 0.413

9.093 ± 0.588

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.607 ± 0.404

3.759 ± 0.277

4.496 ± 0.294

2.769 ± 0.278

5.817 ± 0.496

7.823 ± 0.519

6.452 ± 0.369

6.452 ± 0.217

1.575 ± 0.242

3.531 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski