Amino acid dipepetide frequency for Escherichia phage PA12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.697AlaAla: 8.697 ± 0.864
1.053AlaCys: 1.053 ± 0.312
4.93AlaAsp: 4.93 ± 0.694
8.199AlaGlu: 8.199 ± 0.807
3.545AlaPhe: 3.545 ± 0.604
7.645AlaGly: 7.645 ± 1.146
1.329AlaHis: 1.329 ± 0.38
4.875AlaIle: 4.875 ± 0.708
4.432AlaLys: 4.432 ± 0.503
7.312AlaLeu: 7.312 ± 0.722
3.158AlaMet: 3.158 ± 0.435
2.881AlaAsn: 2.881 ± 0.404
3.102AlaPro: 3.102 ± 0.442
5.373AlaGln: 5.373 ± 0.71
5.595AlaArg: 5.595 ± 0.572
5.65AlaSer: 5.65 ± 0.479
5.65AlaThr: 5.65 ± 0.728
6.537AlaVal: 6.537 ± 0.613
1.828AlaTrp: 1.828 ± 0.301
2.493AlaTyr: 2.493 ± 0.34
0.0AlaXaa: 0.0 ± 0.0
Cys
1.163CysAla: 1.163 ± 0.304
0.332CysCys: 0.332 ± 0.15
0.665CysAsp: 0.665 ± 0.216
0.776CysGlu: 0.776 ± 0.262
0.388CysPhe: 0.388 ± 0.16
0.831CysGly: 0.831 ± 0.297
0.499CysHis: 0.499 ± 0.203
0.609CysIle: 0.609 ± 0.215
0.443CysLys: 0.443 ± 0.196
0.942CysLeu: 0.942 ± 0.282
0.055CysMet: 0.055 ± 0.063
0.277CysAsn: 0.277 ± 0.108
0.499CysPro: 0.499 ± 0.167
0.443CysGln: 0.443 ± 0.165
1.053CysArg: 1.053 ± 0.286
1.108CysSer: 1.108 ± 0.34
0.554CysThr: 0.554 ± 0.193
0.776CysVal: 0.776 ± 0.235
0.166CysTrp: 0.166 ± 0.101
0.277CysTyr: 0.277 ± 0.135
0.0CysXaa: 0.0 ± 0.0
Asp
6.204AspAla: 6.204 ± 0.616
0.72AspCys: 0.72 ± 0.247
4.099AspAsp: 4.099 ± 0.466
4.321AspGlu: 4.321 ± 0.516
1.773AspPhe: 1.773 ± 0.27
4.875AspGly: 4.875 ± 0.54
0.831AspHis: 0.831 ± 0.242
3.324AspIle: 3.324 ± 0.402
4.21AspLys: 4.21 ± 0.407
4.155AspLeu: 4.155 ± 0.416
1.828AspMet: 1.828 ± 0.31
2.714AspAsn: 2.714 ± 0.467
2.493AspPro: 2.493 ± 0.393
1.551AspGln: 1.551 ± 0.362
3.213AspArg: 3.213 ± 0.365
3.379AspSer: 3.379 ± 0.366
2.77AspThr: 2.77 ± 0.398
3.933AspVal: 3.933 ± 0.377
1.108AspTrp: 1.108 ± 0.331
1.717AspTyr: 1.717 ± 0.364
0.0AspXaa: 0.0 ± 0.0
Glu
6.703GluAla: 6.703 ± 0.653
1.163GluCys: 1.163 ± 0.314
2.216GluAsp: 2.216 ± 0.302
4.099GluGlu: 4.099 ± 0.545
2.493GluPhe: 2.493 ± 0.454
3.767GluGly: 3.767 ± 0.61
1.053GluHis: 1.053 ± 0.228
4.099GluIle: 4.099 ± 0.51
5.207GluLys: 5.207 ± 0.726
5.761GluLeu: 5.761 ± 0.645
2.437GluMet: 2.437 ± 0.343
2.991GluAsn: 2.991 ± 0.577
2.105GluPro: 2.105 ± 0.48
4.542GluGln: 4.542 ± 0.54
5.595GluArg: 5.595 ± 0.643
3.656GluSer: 3.656 ± 0.415
3.878GluThr: 3.878 ± 0.669
4.044GluVal: 4.044 ± 0.645
0.886GluTrp: 0.886 ± 0.183
2.105GluTyr: 2.105 ± 0.31
0.0GluXaa: 0.0 ± 0.0
Phe
2.714PheAla: 2.714 ± 0.411
0.443PheCys: 0.443 ± 0.176
1.828PheAsp: 1.828 ± 0.317
1.44PheGlu: 1.44 ± 0.375
0.831PhePhe: 0.831 ± 0.223
2.16PheGly: 2.16 ± 0.302
0.609PheHis: 0.609 ± 0.151
1.773PheIle: 1.773 ± 0.293
1.883PheLys: 1.883 ± 0.337
2.16PheLeu: 2.16 ± 0.369
1.108PheMet: 1.108 ± 0.278
1.385PheAsn: 1.385 ± 0.314
1.163PhePro: 1.163 ± 0.26
0.997PheGln: 0.997 ± 0.275
2.16PheArg: 2.16 ± 0.383
2.881PheSer: 2.881 ± 0.41
2.493PheThr: 2.493 ± 0.362
2.216PheVal: 2.216 ± 0.369
0.831PheTrp: 0.831 ± 0.222
1.108PheTyr: 1.108 ± 0.252
0.0PheXaa: 0.0 ± 0.0
Gly
6.315GlyAla: 6.315 ± 0.911
0.886GlyCys: 0.886 ± 0.289
4.819GlyAsp: 4.819 ± 0.804
6.426GlyGlu: 6.426 ± 1.416
2.548GlyPhe: 2.548 ± 0.38
5.152GlyGly: 5.152 ± 0.683
1.163GlyHis: 1.163 ± 0.259
3.988GlyIle: 3.988 ± 0.588
4.709GlyLys: 4.709 ± 0.967
4.653GlyLeu: 4.653 ± 0.475
2.659GlyMet: 2.659 ± 0.412
2.991GlyAsn: 2.991 ± 0.362
3.878GlyPro: 3.878 ± 2.148
2.77GlyGln: 2.77 ± 0.442
4.155GlyArg: 4.155 ± 0.532
3.878GlySer: 3.878 ± 0.6
3.712GlyThr: 3.712 ± 0.514
5.263GlyVal: 5.263 ± 0.544
1.163GlyTrp: 1.163 ± 0.28
2.604GlyTyr: 2.604 ± 0.403
0.0GlyXaa: 0.0 ± 0.0
His
1.44HisAla: 1.44 ± 0.252
0.166HisCys: 0.166 ± 0.105
0.997HisAsp: 0.997 ± 0.204
0.997HisGlu: 0.997 ± 0.239
0.72HisPhe: 0.72 ± 0.26
1.828HisGly: 1.828 ± 0.408
0.443HisHis: 0.443 ± 0.135
0.609HisIle: 0.609 ± 0.195
1.053HisLys: 1.053 ± 0.298
1.551HisLeu: 1.551 ± 0.413
0.277HisMet: 0.277 ± 0.127
0.886HisAsn: 0.886 ± 0.194
0.886HisPro: 0.886 ± 0.256
0.665HisGln: 0.665 ± 0.229
0.831HisArg: 0.831 ± 0.221
0.72HisSer: 0.72 ± 0.201
1.163HisThr: 1.163 ± 0.282
0.942HisVal: 0.942 ± 0.24
0.443HisTrp: 0.443 ± 0.205
0.499HisTyr: 0.499 ± 0.143
0.0HisXaa: 0.0 ± 0.0
Ile
4.93IleAla: 4.93 ± 0.396
0.997IleCys: 0.997 ± 0.292
4.376IleAsp: 4.376 ± 0.564
3.102IleGlu: 3.102 ± 0.515
0.997IlePhe: 0.997 ± 0.292
3.102IleGly: 3.102 ± 0.477
0.776IleHis: 0.776 ± 0.188
2.16IleIle: 2.16 ± 0.336
3.047IleLys: 3.047 ± 0.405
3.545IleLeu: 3.545 ± 0.607
1.219IleMet: 1.219 ± 0.248
2.991IleAsn: 2.991 ± 0.388
2.327IlePro: 2.327 ± 0.298
1.773IleGln: 1.773 ± 0.232
4.487IleArg: 4.487 ± 0.426
4.044IleSer: 4.044 ± 0.584
4.155IleThr: 4.155 ± 0.695
1.939IleVal: 1.939 ± 0.409
0.388IleTrp: 0.388 ± 0.184
1.496IleTyr: 1.496 ± 0.321
0.0IleXaa: 0.0 ± 0.0
Lys
6.149LysAla: 6.149 ± 0.503
0.388LysCys: 0.388 ± 0.175
3.324LysAsp: 3.324 ± 0.412
3.656LysGlu: 3.656 ± 0.458
0.942LysPhe: 0.942 ± 0.205
5.761LysGly: 5.761 ± 1.109
0.997LysHis: 0.997 ± 0.258
3.379LysIle: 3.379 ± 0.45
3.767LysLys: 3.767 ± 0.488
4.93LysLeu: 4.93 ± 0.433
1.883LysMet: 1.883 ± 0.308
3.656LysAsn: 3.656 ± 0.48
2.659LysPro: 2.659 ± 0.341
3.102LysGln: 3.102 ± 0.547
2.991LysArg: 2.991 ± 0.437
2.936LysSer: 2.936 ± 0.371
3.268LysThr: 3.268 ± 0.446
3.102LysVal: 3.102 ± 0.479
0.831LysTrp: 0.831 ± 0.245
1.717LysTyr: 1.717 ± 0.319
0.0LysXaa: 0.0 ± 0.0
Leu
7.977LeuAla: 7.977 ± 0.803
1.274LeuCys: 1.274 ± 0.369
3.822LeuAsp: 3.822 ± 0.403
4.265LeuGlu: 4.265 ± 0.549
2.825LeuPhe: 2.825 ± 0.399
4.21LeuGly: 4.21 ± 0.421
1.385LeuHis: 1.385 ± 0.3
3.988LeuIle: 3.988 ± 0.541
4.653LeuLys: 4.653 ± 0.529
6.149LeuLeu: 6.149 ± 0.491
2.05LeuMet: 2.05 ± 0.398
4.321LeuAsn: 4.321 ± 0.536
3.712LeuPro: 3.712 ± 0.458
3.324LeuGln: 3.324 ± 0.746
5.484LeuArg: 5.484 ± 0.593
4.764LeuSer: 4.764 ± 0.471
4.764LeuThr: 4.764 ± 0.477
4.598LeuVal: 4.598 ± 0.384
0.942LeuTrp: 0.942 ± 0.329
2.327LeuTyr: 2.327 ± 0.303
0.0LeuXaa: 0.0 ± 0.0
Met
3.268MetAla: 3.268 ± 0.422
0.0MetCys: 0.0 ± 0.0
1.385MetAsp: 1.385 ± 0.249
1.717MetGlu: 1.717 ± 0.341
0.831MetPhe: 0.831 ± 0.209
1.551MetGly: 1.551 ± 0.338
0.332MetHis: 0.332 ± 0.116
0.997MetIle: 0.997 ± 0.204
2.16MetLys: 2.16 ± 0.368
2.16MetLeu: 2.16 ± 0.342
0.72MetMet: 0.72 ± 0.193
1.496MetAsn: 1.496 ± 0.325
1.773MetPro: 1.773 ± 0.315
1.551MetGln: 1.551 ± 0.272
1.496MetArg: 1.496 ± 0.252
2.16MetSer: 2.16 ± 0.327
2.77MetThr: 2.77 ± 0.43
1.329MetVal: 1.329 ± 0.302
0.222MetTrp: 0.222 ± 0.124
0.443MetTyr: 0.443 ± 0.205
0.0MetXaa: 0.0 ± 0.0
Asn
4.653AsnAla: 4.653 ± 0.764
0.499AsnCys: 0.499 ± 0.189
2.493AsnAsp: 2.493 ± 0.362
2.991AsnGlu: 2.991 ± 0.334
1.108AsnPhe: 1.108 ± 0.242
3.158AsnGly: 3.158 ± 0.495
1.385AsnHis: 1.385 ± 0.323
2.659AsnIle: 2.659 ± 0.386
2.271AsnLys: 2.271 ± 0.296
3.379AsnLeu: 3.379 ± 0.465
0.997AsnMet: 0.997 ± 0.239
1.994AsnAsn: 1.994 ± 0.391
1.828AsnPro: 1.828 ± 0.277
2.16AsnGln: 2.16 ± 0.336
2.77AsnArg: 2.77 ± 0.421
2.714AsnSer: 2.714 ± 0.397
2.548AsnThr: 2.548 ± 0.422
2.16AsnVal: 2.16 ± 0.357
0.388AsnTrp: 0.388 ± 0.13
1.274AsnTyr: 1.274 ± 0.338
0.0AsnXaa: 0.0 ± 0.0
Pro
3.435ProAla: 3.435 ± 0.588
0.332ProCys: 0.332 ± 0.118
4.321ProAsp: 4.321 ± 0.463
4.986ProGlu: 4.986 ± 0.841
1.329ProPhe: 1.329 ± 0.219
3.435ProGly: 3.435 ± 0.494
0.665ProHis: 0.665 ± 0.234
1.053ProIle: 1.053 ± 0.262
2.936ProLys: 2.936 ± 0.751
2.936ProLeu: 2.936 ± 0.444
0.886ProMet: 0.886 ± 0.202
0.776ProAsn: 0.776 ± 0.185
1.385ProPro: 1.385 ± 0.266
2.437ProGln: 2.437 ± 0.514
1.828ProArg: 1.828 ± 0.355
2.659ProSer: 2.659 ± 0.401
1.883ProThr: 1.883 ± 0.328
4.21ProVal: 4.21 ± 0.497
0.609ProTrp: 0.609 ± 0.171
1.496ProTyr: 1.496 ± 0.32
0.0ProXaa: 0.0 ± 0.0
Gln
4.93GlnAla: 4.93 ± 0.63
0.776GlnCys: 0.776 ± 0.252
2.437GlnAsp: 2.437 ± 0.427
3.213GlnGlu: 3.213 ± 0.404
1.44GlnPhe: 1.44 ± 0.286
3.158GlnGly: 3.158 ± 0.652
0.776GlnHis: 0.776 ± 0.195
2.604GlnIle: 2.604 ± 0.504
3.49GlnLys: 3.49 ± 0.543
3.435GlnLeu: 3.435 ± 0.374
1.219GlnMet: 1.219 ± 0.277
1.828GlnAsn: 1.828 ± 0.35
2.16GlnPro: 2.16 ± 0.44
3.988GlnGln: 3.988 ± 0.896
3.379GlnArg: 3.379 ± 0.588
2.77GlnSer: 2.77 ± 0.311
2.105GlnThr: 2.105 ± 0.376
2.327GlnVal: 2.327 ± 0.502
0.609GlnTrp: 0.609 ± 0.171
1.329GlnTyr: 1.329 ± 0.289
0.0GlnXaa: 0.0 ± 0.0
Arg
4.875ArgAla: 4.875 ± 0.425
0.277ArgCys: 0.277 ± 0.124
4.21ArgAsp: 4.21 ± 0.638
5.318ArgGlu: 5.318 ± 0.702
2.271ArgPhe: 2.271 ± 0.348
5.041ArgGly: 5.041 ± 0.893
1.606ArgHis: 1.606 ± 0.247
3.601ArgIle: 3.601 ± 0.425
4.21ArgLys: 4.21 ± 0.626
5.096ArgLeu: 5.096 ± 0.48
2.05ArgMet: 2.05 ± 0.339
3.213ArgAsn: 3.213 ± 0.443
2.05ArgPro: 2.05 ± 0.358
2.991ArgGln: 2.991 ± 0.393
5.429ArgArg: 5.429 ± 0.598
3.656ArgSer: 3.656 ± 0.493
3.435ArgThr: 3.435 ± 0.569
3.712ArgVal: 3.712 ± 0.495
1.108ArgTrp: 1.108 ± 0.232
2.16ArgTyr: 2.16 ± 0.455
0.0ArgXaa: 0.0 ± 0.0
Ser
5.706SerAla: 5.706 ± 0.539
0.72SerCys: 0.72 ± 0.211
3.601SerAsp: 3.601 ± 0.468
4.044SerGlu: 4.044 ± 0.484
1.662SerPhe: 1.662 ± 0.289
5.65SerGly: 5.65 ± 0.556
0.831SerHis: 0.831 ± 0.214
2.327SerIle: 2.327 ± 0.339
2.659SerLys: 2.659 ± 0.487
5.54SerLeu: 5.54 ± 0.697
1.44SerMet: 1.44 ± 0.303
2.548SerAsn: 2.548 ± 0.415
3.268SerPro: 3.268 ± 0.424
3.324SerGln: 3.324 ± 0.419
4.432SerArg: 4.432 ± 0.744
3.158SerSer: 3.158 ± 0.706
3.047SerThr: 3.047 ± 0.423
4.155SerVal: 4.155 ± 0.519
0.886SerTrp: 0.886 ± 0.266
1.717SerTyr: 1.717 ± 0.414
0.0SerXaa: 0.0 ± 0.0
Thr
5.595ThrAla: 5.595 ± 0.532
0.499ThrCys: 0.499 ± 0.208
3.435ThrAsp: 3.435 ± 0.426
3.601ThrGlu: 3.601 ± 0.381
2.327ThrPhe: 2.327 ± 0.482
5.983ThrGly: 5.983 ± 0.77
0.942ThrHis: 0.942 ± 0.252
3.49ThrIle: 3.49 ± 0.457
2.825ThrLys: 2.825 ± 0.395
4.986ThrLeu: 4.986 ± 0.479
0.831ThrMet: 0.831 ± 0.214
1.551ThrAsn: 1.551 ± 0.225
3.601ThrPro: 3.601 ± 0.363
2.105ThrGln: 2.105 ± 0.385
2.714ThrArg: 2.714 ± 0.36
3.324ThrSer: 3.324 ± 0.443
3.379ThrThr: 3.379 ± 0.493
4.376ThrVal: 4.376 ± 0.518
0.886ThrTrp: 0.886 ± 0.225
1.108ThrTyr: 1.108 ± 0.289
0.0ThrXaa: 0.0 ± 0.0
Val
5.706ValAla: 5.706 ± 0.562
0.942ValCys: 0.942 ± 0.271
3.878ValAsp: 3.878 ± 0.463
3.435ValGlu: 3.435 ± 0.387
2.16ValPhe: 2.16 ± 0.328
3.49ValGly: 3.49 ± 0.519
0.72ValHis: 0.72 ± 0.156
3.878ValIle: 3.878 ± 0.531
3.49ValLys: 3.49 ± 0.42
5.263ValLeu: 5.263 ± 0.625
1.717ValMet: 1.717 ± 0.292
3.102ValAsn: 3.102 ± 0.369
2.77ValPro: 2.77 ± 0.342
2.382ValGln: 2.382 ± 0.345
4.986ValArg: 4.986 ± 1.06
4.598ValSer: 4.598 ± 0.472
3.822ValThr: 3.822 ± 0.468
4.376ValVal: 4.376 ± 0.433
0.665ValTrp: 0.665 ± 0.192
1.606ValTyr: 1.606 ± 0.262
0.0ValXaa: 0.0 ± 0.0
Trp
0.942TrpAla: 0.942 ± 0.285
0.111TrpCys: 0.111 ± 0.066
0.665TrpAsp: 0.665 ± 0.237
0.609TrpGlu: 0.609 ± 0.168
0.72TrpPhe: 0.72 ± 0.175
0.609TrpGly: 0.609 ± 0.19
0.332TrpHis: 0.332 ± 0.13
0.776TrpIle: 0.776 ± 0.202
0.997TrpLys: 0.997 ± 0.217
1.44TrpLeu: 1.44 ± 0.382
0.886TrpMet: 0.886 ± 0.235
0.665TrpAsn: 0.665 ± 0.198
0.665TrpPro: 0.665 ± 0.202
0.997TrpGln: 0.997 ± 0.222
1.219TrpArg: 1.219 ± 0.298
0.886TrpSer: 0.886 ± 0.222
0.609TrpThr: 0.609 ± 0.175
1.108TrpVal: 1.108 ± 0.305
0.332TrpTrp: 0.332 ± 0.156
0.388TrpTyr: 0.388 ± 0.146
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.881TyrAla: 2.881 ± 0.472
0.277TyrCys: 0.277 ± 0.152
1.717TyrAsp: 1.717 ± 0.301
1.606TyrGlu: 1.606 ± 0.368
1.274TyrPhe: 1.274 ± 0.249
2.327TyrGly: 2.327 ± 0.445
0.443TyrHis: 0.443 ± 0.159
1.662TyrIle: 1.662 ± 0.315
0.997TyrLys: 0.997 ± 0.228
1.44TyrLeu: 1.44 ± 0.297
0.886TyrMet: 0.886 ± 0.241
1.219TyrAsn: 1.219 ± 0.263
1.274TyrPro: 1.274 ± 0.235
1.496TyrGln: 1.496 ± 0.287
2.493TyrArg: 2.493 ± 0.52
1.773TyrSer: 1.773 ± 0.312
1.551TyrThr: 1.551 ± 0.401
1.883TyrVal: 1.883 ± 0.281
0.665TyrTrp: 0.665 ± 0.169
0.942TyrTyr: 0.942 ± 0.218
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (18053 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski