Amino acid dipepetide frequency for Mycobacterium phage Wee

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.745AlaAla: 14.745 ± 1.715
0.948AlaCys: 0.948 ± 0.212
7.214AlaAsp: 7.214 ± 0.602
7.741AlaGlu: 7.741 ± 0.715
2.738AlaPhe: 2.738 ± 0.37
10.479AlaGly: 10.479 ± 1.253
2.422AlaHis: 2.422 ± 0.423
4.16AlaIle: 4.16 ± 0.484
4.265AlaLys: 4.265 ± 0.49
7.952AlaLeu: 7.952 ± 0.762
3.054AlaMet: 3.054 ± 0.465
2.528AlaAsn: 2.528 ± 0.33
5.424AlaPro: 5.424 ± 0.531
3.107AlaGln: 3.107 ± 0.447
7.32AlaArg: 7.32 ± 0.699
5.371AlaSer: 5.371 ± 0.586
5.635AlaThr: 5.635 ± 0.542
6.951AlaVal: 6.951 ± 0.676
2.896AlaTrp: 2.896 ± 0.457
2.001AlaTyr: 2.001 ± 0.299
0.0AlaXaa: 0.0 ± 0.0
Cys
1.001CysAla: 1.001 ± 0.257
0.105CysCys: 0.105 ± 0.073
1.474CysAsp: 1.474 ± 0.369
0.579CysGlu: 0.579 ± 0.157
0.316CysPhe: 0.316 ± 0.117
1.474CysGly: 1.474 ± 0.296
0.211CysHis: 0.211 ± 0.092
0.316CysIle: 0.316 ± 0.146
0.369CysLys: 0.369 ± 0.134
0.895CysLeu: 0.895 ± 0.245
0.263CysMet: 0.263 ± 0.107
0.316CysAsn: 0.316 ± 0.128
1.106CysPro: 1.106 ± 0.315
0.316CysGln: 0.316 ± 0.132
0.632CysArg: 0.632 ± 0.208
0.79CysSer: 0.79 ± 0.21
0.632CysThr: 0.632 ± 0.171
0.737CysVal: 0.737 ± 0.195
0.369CysTrp: 0.369 ± 0.12
0.211CysTyr: 0.211 ± 0.113
0.0CysXaa: 0.0 ± 0.0
Asp
6.898AspAla: 6.898 ± 0.663
1.053AspCys: 1.053 ± 0.244
4.95AspAsp: 4.95 ± 0.548
3.633AspGlu: 3.633 ± 0.498
2.001AspPhe: 2.001 ± 0.309
7.32AspGly: 7.32 ± 0.577
1.316AspHis: 1.316 ± 0.271
2.159AspIle: 2.159 ± 0.313
1.79AspLys: 1.79 ± 0.338
5.898AspLeu: 5.898 ± 0.596
1.211AspMet: 1.211 ± 0.272
1.58AspAsn: 1.58 ± 0.328
4.739AspPro: 4.739 ± 0.571
2.264AspGln: 2.264 ± 0.328
5.371AspArg: 5.371 ± 0.725
4.002AspSer: 4.002 ± 0.492
4.897AspThr: 4.897 ± 0.464
4.265AspVal: 4.265 ± 0.518
1.422AspTrp: 1.422 ± 0.284
2.054AspTyr: 2.054 ± 0.332
0.0AspXaa: 0.0 ± 0.0
Glu
6.74GluAla: 6.74 ± 0.717
1.001GluCys: 1.001 ± 0.256
2.58GluAsp: 2.58 ± 0.366
3.002GluGlu: 3.002 ± 0.53
2.528GluPhe: 2.528 ± 0.322
2.896GluGly: 2.896 ± 0.421
1.79GluHis: 1.79 ± 0.426
2.264GluIle: 2.264 ± 0.356
1.79GluLys: 1.79 ± 0.281
5.582GluLeu: 5.582 ± 0.71
1.843GluMet: 1.843 ± 0.317
2.001GluAsn: 2.001 ± 0.288
2.738GluPro: 2.738 ± 0.456
2.475GluGln: 2.475 ± 0.412
5.371GluArg: 5.371 ± 0.605
2.949GluSer: 2.949 ± 0.418
3.949GluThr: 3.949 ± 0.629
3.739GluVal: 3.739 ± 0.424
1.422GluTrp: 1.422 ± 0.227
1.843GluTyr: 1.843 ± 0.365
0.0GluXaa: 0.0 ± 0.0
Phe
3.212PheAla: 3.212 ± 0.45
0.211PheCys: 0.211 ± 0.091
2.791PheAsp: 2.791 ± 0.414
1.79PheGlu: 1.79 ± 0.307
0.895PhePhe: 0.895 ± 0.268
2.896PheGly: 2.896 ± 0.495
0.316PheHis: 0.316 ± 0.138
1.159PheIle: 1.159 ± 0.322
1.053PheLys: 1.053 ± 0.253
1.843PheLeu: 1.843 ± 0.252
0.948PheMet: 0.948 ± 0.225
1.264PheAsn: 1.264 ± 0.291
1.632PhePro: 1.632 ± 0.263
1.001PheGln: 1.001 ± 0.306
1.527PheArg: 1.527 ± 0.241
1.632PheSer: 1.632 ± 0.283
2.528PheThr: 2.528 ± 0.372
2.001PheVal: 2.001 ± 0.266
0.579PheTrp: 0.579 ± 0.153
0.843PheTyr: 0.843 ± 0.258
0.0PheXaa: 0.0 ± 0.0
Gly
9.268GlyAla: 9.268 ± 1.111
1.053GlyCys: 1.053 ± 0.228
6.477GlyAsp: 6.477 ± 0.559
3.739GlyGlu: 3.739 ± 0.509
2.949GlyPhe: 2.949 ± 0.402
10.69GlyGly: 10.69 ± 2.359
2.054GlyHis: 2.054 ± 0.306
4.213GlyIle: 4.213 ± 0.588
2.422GlyLys: 2.422 ± 0.328
5.898GlyLeu: 5.898 ± 0.569
2.264GlyMet: 2.264 ± 0.435
2.949GlyAsn: 2.949 ± 0.473
4.002GlyPro: 4.002 ± 0.506
2.264GlyGln: 2.264 ± 0.521
5.424GlyArg: 5.424 ± 0.716
5.845GlySer: 5.845 ± 0.992
6.635GlyThr: 6.635 ± 0.856
5.477GlyVal: 5.477 ± 0.492
2.738GlyTrp: 2.738 ± 0.412
2.212GlyTyr: 2.212 ± 0.377
0.0GlyXaa: 0.0 ± 0.0
His
1.948HisAla: 1.948 ± 0.376
0.369HisCys: 0.369 ± 0.173
1.58HisAsp: 1.58 ± 0.333
1.211HisGlu: 1.211 ± 0.268
0.316HisPhe: 0.316 ± 0.114
1.632HisGly: 1.632 ± 0.283
0.895HisHis: 0.895 ± 0.216
1.264HisIle: 1.264 ± 0.254
1.001HisLys: 1.001 ± 0.206
1.264HisLeu: 1.264 ± 0.234
0.474HisMet: 0.474 ± 0.128
1.001HisAsn: 1.001 ± 0.216
1.896HisPro: 1.896 ± 0.295
0.843HisGln: 0.843 ± 0.22
2.317HisArg: 2.317 ± 0.4
0.685HisSer: 0.685 ± 0.183
1.527HisThr: 1.527 ± 0.335
1.264HisVal: 1.264 ± 0.339
0.421HisTrp: 0.421 ± 0.145
0.79HisTyr: 0.79 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
5.319IleAla: 5.319 ± 0.554
0.632IleCys: 0.632 ± 0.212
4.16IleAsp: 4.16 ± 0.45
3.476IleGlu: 3.476 ± 0.373
0.737IlePhe: 0.737 ± 0.228
3.265IleGly: 3.265 ± 0.462
1.316IleHis: 1.316 ± 0.285
1.264IleIle: 1.264 ± 0.271
1.106IleLys: 1.106 ± 0.24
2.001IleLeu: 2.001 ± 0.337
0.316IleMet: 0.316 ± 0.131
1.843IleAsn: 1.843 ± 0.279
2.949IlePro: 2.949 ± 0.337
1.474IleGln: 1.474 ± 0.278
2.791IleArg: 2.791 ± 0.454
2.264IleSer: 2.264 ± 0.391
3.528IleThr: 3.528 ± 0.475
2.844IleVal: 2.844 ± 0.382
0.843IleTrp: 0.843 ± 0.216
0.79IleTyr: 0.79 ± 0.196
0.0IleXaa: 0.0 ± 0.0
Lys
3.949LysAla: 3.949 ± 0.507
0.263LysCys: 0.263 ± 0.101
1.422LysAsp: 1.422 ± 0.292
1.474LysGlu: 1.474 ± 0.254
1.106LysPhe: 1.106 ± 0.205
2.422LysGly: 2.422 ± 0.314
1.053LysHis: 1.053 ± 0.258
1.106LysIle: 1.106 ± 0.231
1.369LysLys: 1.369 ± 0.373
2.264LysLeu: 2.264 ± 0.405
0.474LysMet: 0.474 ± 0.137
0.737LysAsn: 0.737 ± 0.222
2.37LysPro: 2.37 ± 0.409
1.632LysGln: 1.632 ± 0.21
2.475LysArg: 2.475 ± 0.354
1.843LysSer: 1.843 ± 0.3
2.58LysThr: 2.58 ± 0.407
2.633LysVal: 2.633 ± 0.408
0.79LysTrp: 0.79 ± 0.21
0.948LysTyr: 0.948 ± 0.209
0.0LysXaa: 0.0 ± 0.0
Leu
7.899LeuAla: 7.899 ± 0.826
0.843LeuCys: 0.843 ± 0.255
5.319LeuAsp: 5.319 ± 0.653
3.686LeuGlu: 3.686 ± 0.483
2.475LeuPhe: 2.475 ± 0.291
5.266LeuGly: 5.266 ± 0.542
1.211LeuHis: 1.211 ± 0.256
3.318LeuIle: 3.318 ± 0.461
1.948LeuLys: 1.948 ± 0.266
4.371LeuLeu: 4.371 ± 0.49
1.474LeuMet: 1.474 ± 0.273
2.422LeuAsn: 2.422 ± 0.402
5.213LeuPro: 5.213 ± 0.761
2.633LeuGln: 2.633 ± 0.457
4.687LeuArg: 4.687 ± 0.566
5.371LeuSer: 5.371 ± 0.481
5.161LeuThr: 5.161 ± 0.506
5.213LeuVal: 5.213 ± 0.532
1.211LeuTrp: 1.211 ± 0.234
2.264LeuTyr: 2.264 ± 0.31
0.0LeuXaa: 0.0 ± 0.0
Met
2.475MetAla: 2.475 ± 0.356
0.211MetCys: 0.211 ± 0.105
1.211MetAsp: 1.211 ± 0.281
0.895MetGlu: 0.895 ± 0.22
0.737MetPhe: 0.737 ± 0.227
1.738MetGly: 1.738 ± 0.28
0.211MetHis: 0.211 ± 0.1
0.948MetIle: 0.948 ± 0.235
0.79MetLys: 0.79 ± 0.264
1.632MetLeu: 1.632 ± 0.285
0.579MetMet: 0.579 ± 0.199
0.79MetAsn: 0.79 ± 0.191
1.527MetPro: 1.527 ± 0.3
0.421MetGln: 0.421 ± 0.121
1.79MetArg: 1.79 ± 0.268
2.633MetSer: 2.633 ± 0.342
2.317MetThr: 2.317 ± 0.326
1.474MetVal: 1.474 ± 0.353
0.369MetTrp: 0.369 ± 0.14
0.263MetTyr: 0.263 ± 0.107
0.0MetXaa: 0.0 ± 0.0
Asn
3.265AsnAla: 3.265 ± 0.368
0.211AsnCys: 0.211 ± 0.096
1.843AsnAsp: 1.843 ± 0.289
2.001AsnGlu: 2.001 ± 0.311
0.843AsnPhe: 0.843 ± 0.32
4.107AsnGly: 4.107 ± 0.531
0.79AsnHis: 0.79 ± 0.17
1.632AsnIle: 1.632 ± 0.484
1.001AsnLys: 1.001 ± 0.255
2.264AsnLeu: 2.264 ± 0.345
0.474AsnMet: 0.474 ± 0.149
1.685AsnAsn: 1.685 ± 0.356
2.317AsnPro: 2.317 ± 0.348
0.948AsnGln: 0.948 ± 0.311
2.054AsnArg: 2.054 ± 0.409
1.843AsnSer: 1.843 ± 0.279
2.054AsnThr: 2.054 ± 0.357
1.738AsnVal: 1.738 ± 0.301
0.737AsnTrp: 0.737 ± 0.148
0.579AsnTyr: 0.579 ± 0.156
0.0AsnXaa: 0.0 ± 0.0
Pro
5.055ProAla: 5.055 ± 0.494
0.579ProCys: 0.579 ± 0.163
4.581ProAsp: 4.581 ± 0.515
4.265ProGlu: 4.265 ± 0.466
1.632ProPhe: 1.632 ± 0.278
7.004ProGly: 7.004 ± 0.687
1.474ProHis: 1.474 ± 0.291
2.159ProIle: 2.159 ± 0.272
2.054ProLys: 2.054 ± 0.39
4.529ProLeu: 4.529 ± 0.559
1.58ProMet: 1.58 ± 0.315
2.106ProAsn: 2.106 ± 0.323
3.476ProPro: 3.476 ± 0.561
1.79ProGln: 1.79 ± 0.349
3.476ProArg: 3.476 ± 0.539
3.002ProSer: 3.002 ± 0.347
3.528ProThr: 3.528 ± 0.365
4.845ProVal: 4.845 ± 0.588
0.843ProTrp: 0.843 ± 0.2
1.369ProTyr: 1.369 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
4.318GlnAla: 4.318 ± 0.637
0.474GlnCys: 0.474 ± 0.173
1.58GlnAsp: 1.58 ± 0.26
1.474GlnGlu: 1.474 ± 0.296
1.053GlnPhe: 1.053 ± 0.203
2.37GlnGly: 2.37 ± 0.477
0.632GlnHis: 0.632 ± 0.217
1.685GlnIle: 1.685 ± 0.28
1.316GlnLys: 1.316 ± 0.267
2.686GlnLeu: 2.686 ± 0.452
0.474GlnMet: 0.474 ± 0.165
0.843GlnAsn: 0.843 ± 0.269
2.317GlnPro: 2.317 ± 0.37
0.948GlnGln: 0.948 ± 0.189
2.528GlnArg: 2.528 ± 0.328
2.001GlnSer: 2.001 ± 0.377
2.001GlnThr: 2.001 ± 0.321
2.37GlnVal: 2.37 ± 0.349
0.79GlnTrp: 0.79 ± 0.185
0.948GlnTyr: 0.948 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
6.688ArgAla: 6.688 ± 0.592
1.159ArgCys: 1.159 ± 0.283
4.581ArgAsp: 4.581 ± 0.558
4.739ArgGlu: 4.739 ± 0.645
2.264ArgPhe: 2.264 ± 0.458
4.423ArgGly: 4.423 ± 0.415
1.58ArgHis: 1.58 ± 0.355
3.739ArgIle: 3.739 ± 0.433
2.528ArgLys: 2.528 ± 0.462
4.897ArgLeu: 4.897 ± 0.544
2.475ArgMet: 2.475 ± 0.366
2.37ArgAsn: 2.37 ± 0.451
3.686ArgPro: 3.686 ± 0.484
2.054ArgGln: 2.054 ± 0.351
5.74ArgArg: 5.74 ± 0.855
3.897ArgSer: 3.897 ± 0.393
3.581ArgThr: 3.581 ± 0.503
5.529ArgVal: 5.529 ± 0.567
2.106ArgTrp: 2.106 ± 0.425
1.79ArgTyr: 1.79 ± 0.291
0.0ArgXaa: 0.0 ± 0.0
Ser
6.003SerAla: 6.003 ± 0.901
0.632SerCys: 0.632 ± 0.233
3.581SerAsp: 3.581 ± 0.443
3.16SerGlu: 3.16 ± 0.456
2.106SerPhe: 2.106 ± 0.418
6.477SerGly: 6.477 ± 0.749
1.053SerHis: 1.053 ± 0.197
2.949SerIle: 2.949 ± 0.402
2.686SerLys: 2.686 ± 0.414
4.423SerLeu: 4.423 ± 0.478
1.316SerMet: 1.316 ± 0.289
2.054SerAsn: 2.054 ± 0.353
3.16SerPro: 3.16 ± 0.361
1.632SerGln: 1.632 ± 0.277
3.739SerArg: 3.739 ± 0.396
3.212SerSer: 3.212 ± 0.538
3.212SerThr: 3.212 ± 0.441
4.16SerVal: 4.16 ± 0.446
1.474SerTrp: 1.474 ± 0.265
1.422SerTyr: 1.422 ± 0.218
0.0SerXaa: 0.0 ± 0.0
Thr
6.372ThrAla: 6.372 ± 0.643
0.632ThrCys: 0.632 ± 0.19
4.529ThrAsp: 4.529 ± 0.58
3.686ThrGlu: 3.686 ± 0.439
1.843ThrPhe: 1.843 ± 0.329
5.951ThrGly: 5.951 ± 0.609
1.948ThrHis: 1.948 ± 0.368
3.37ThrIle: 3.37 ± 0.401
2.159ThrLys: 2.159 ± 0.383
4.687ThrLeu: 4.687 ± 0.497
1.316ThrMet: 1.316 ± 0.233
2.475ThrAsn: 2.475 ± 0.38
4.476ThrPro: 4.476 ± 0.544
2.001ThrGln: 2.001 ± 0.313
4.16ThrArg: 4.16 ± 0.44
4.055ThrSer: 4.055 ± 0.407
5.319ThrThr: 5.319 ± 0.693
5.74ThrVal: 5.74 ± 0.571
1.159ThrTrp: 1.159 ± 0.304
1.843ThrTyr: 1.843 ± 0.286
0.0ThrXaa: 0.0 ± 0.0
Val
7.162ValAla: 7.162 ± 0.476
1.106ValCys: 1.106 ± 0.229
5.582ValAsp: 5.582 ± 0.535
4.687ValGlu: 4.687 ± 0.597
2.264ValPhe: 2.264 ± 0.335
5.213ValGly: 5.213 ± 0.593
1.422ValHis: 1.422 ± 0.296
2.949ValIle: 2.949 ± 0.436
1.896ValLys: 1.896 ± 0.296
5.055ValLeu: 5.055 ± 0.518
1.316ValMet: 1.316 ± 0.209
2.159ValAsn: 2.159 ± 0.332
4.107ValPro: 4.107 ± 0.404
3.002ValGln: 3.002 ± 0.37
4.318ValArg: 4.318 ± 0.589
4.529ValSer: 4.529 ± 0.501
5.161ValThr: 5.161 ± 0.489
6.74ValVal: 6.74 ± 0.768
1.948ValTrp: 1.948 ± 0.399
1.422ValTyr: 1.422 ± 0.263
0.0ValXaa: 0.0 ± 0.0
Trp
2.001TrpAla: 2.001 ± 0.265
0.316TrpCys: 0.316 ± 0.147
1.632TrpAsp: 1.632 ± 0.258
1.316TrpGlu: 1.316 ± 0.344
0.632TrpPhe: 0.632 ± 0.171
1.106TrpGly: 1.106 ± 0.274
0.632TrpHis: 0.632 ± 0.191
1.053TrpIle: 1.053 ± 0.216
0.737TrpLys: 0.737 ± 0.17
2.054TrpLeu: 2.054 ± 0.364
0.895TrpMet: 0.895 ± 0.221
0.579TrpAsn: 0.579 ± 0.22
1.001TrpPro: 1.001 ± 0.253
1.159TrpGln: 1.159 ± 0.3
2.106TrpArg: 2.106 ± 0.37
1.527TrpSer: 1.527 ± 0.332
1.738TrpThr: 1.738 ± 0.303
2.054TrpVal: 2.054 ± 0.423
0.948TrpTrp: 0.948 ± 0.2
0.527TrpTyr: 0.527 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.633TyrAla: 2.633 ± 0.374
0.316TyrCys: 0.316 ± 0.131
1.738TyrAsp: 1.738 ± 0.394
1.948TyrGlu: 1.948 ± 0.26
0.685TyrPhe: 0.685 ± 0.172
1.843TyrGly: 1.843 ± 0.348
0.369TyrHis: 0.369 ± 0.123
1.264TyrIle: 1.264 ± 0.234
0.632TyrLys: 0.632 ± 0.199
1.79TyrLeu: 1.79 ± 0.282
0.211TyrMet: 0.211 ± 0.116
0.685TyrAsn: 0.685 ± 0.18
1.211TyrPro: 1.211 ± 0.208
0.843TyrGln: 0.843 ± 0.191
2.001TyrArg: 2.001 ± 0.368
1.001TyrSer: 1.001 ± 0.268
1.738TyrThr: 1.738 ± 0.356
2.264TyrVal: 2.264 ± 0.37
0.948TyrTrp: 0.948 ± 0.203
0.685TyrTyr: 0.685 ± 0.16
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (18991 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski