Bacillus sp. FJAT-21945

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4788 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M0X5L2|A0A0M0X5L2_9BACI MFS transporter OS=Bacillus sp. FJAT-21945 OX=1581033 GN=AMS60_01185 PE=4 SV=1
MM1 pKa = 6.18YY2 pKa = 8.11TKK4 pKa = 10.61KK5 pKa = 10.58EE6 pKa = 3.79IEE8 pKa = 4.07TQIIEE13 pKa = 4.66LISDD17 pKa = 3.66ISEE20 pKa = 3.73QDD22 pKa = 2.86INYY25 pKa = 10.01SDD27 pKa = 4.05IYY29 pKa = 11.13TPFTEE34 pKa = 4.81LGVDD38 pKa = 3.18SLMALEE44 pKa = 4.5LSVHH48 pKa = 6.04IEE50 pKa = 3.94RR51 pKa = 11.84EE52 pKa = 3.7YY53 pKa = 11.42DD54 pKa = 3.5VYY56 pKa = 11.43LDD58 pKa = 4.44EE59 pKa = 6.51DD60 pKa = 4.18DD61 pKa = 6.55LMTLTCINDD70 pKa = 3.18VFLIINDD77 pKa = 3.95RR78 pKa = 11.84LL79 pKa = 3.48

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M0WDA9|A0A0M0WDA9_9BACI Flagellar basal body protein OS=Bacillus sp. FJAT-21945 OX=1581033 GN=AMS60_21385 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.29QPSKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.11RR12 pKa = 11.84SKK14 pKa = 9.13VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTPNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4788

0

4788

1362251

26

1472

284.5

32.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.749 ± 0.033

0.757 ± 0.01

4.906 ± 0.026

7.558 ± 0.042

4.726 ± 0.031

6.774 ± 0.034

2.031 ± 0.016

8.474 ± 0.04

7.238 ± 0.031

9.861 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.018

4.644 ± 0.026

3.529 ± 0.019

3.561 ± 0.024

3.87 ± 0.026

6.101 ± 0.026

5.171 ± 0.023

6.655 ± 0.026

1.027 ± 0.014

3.546 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski