Burkholderia sp. ST111

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; unclassified Burkholderia

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5742 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1KLX1|A0A0N1KLX1_9BURK Oxidoreductase OS=Burkholderia sp. ST111 OX=1682204 GN=ADM96_00010 PE=3 SV=1
MM1 pKa = 6.97ICGWIYY7 pKa = 11.21DD8 pKa = 4.11EE9 pKa = 4.7VAGLPEE15 pKa = 4.29EE16 pKa = 5.39GIAPGTRR23 pKa = 11.84WADD26 pKa = 3.39VPADD30 pKa = 3.83WRR32 pKa = 11.84CPLCDD37 pKa = 3.32VGKK40 pKa = 10.7EE41 pKa = 3.91DD42 pKa = 3.78FALVEE47 pKa = 4.41FF48 pKa = 4.99

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0ZFE3|A0A0N0ZFE3_9BURK FAD-binding oxidoreductase OS=Burkholderia sp. ST111 OX=1682204 GN=ADM96_20955 PE=4 SV=1
MM1 pKa = 6.79VASGRR6 pKa = 11.84RR7 pKa = 11.84VQVNRR12 pKa = 11.84QAPGRR17 pKa = 11.84VRR19 pKa = 11.84RR20 pKa = 11.84GLKK23 pKa = 10.28DD24 pKa = 3.37RR25 pKa = 11.84FPASGMLAPRR35 pKa = 11.84AGRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84IGRR43 pKa = 11.84RR44 pKa = 11.84GAKK47 pKa = 9.77GPAVRR52 pKa = 11.84RR53 pKa = 11.84AGLRR57 pKa = 11.84AVAIVLRR64 pKa = 11.84VGTRR68 pKa = 11.84VNVRR72 pKa = 11.84RR73 pKa = 11.84AGLKK77 pKa = 9.72VVAIVANVRR86 pKa = 3.36

Molecular weight: 9.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5742

0

5742

1645715

27

1928

286.6

31.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.276 ± 0.046

0.943 ± 0.011

5.335 ± 0.027

5.193 ± 0.032

3.79 ± 0.022

8.036 ± 0.034

2.344 ± 0.016

4.9 ± 0.024

3.259 ± 0.025

10.223 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.018

2.89 ± 0.021

4.957 ± 0.024

3.663 ± 0.02

6.709 ± 0.031

5.873 ± 0.024

5.541 ± 0.024

7.79 ± 0.026

1.378 ± 0.014

2.492 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski