Methanomassiliicoccales archaeon RumEn M2

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Methanomassiliicoccales; unclassified Methanomassiliicoccales

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0Q4BBW1|A0A0Q4BBW1_9ARCH Modification methylase HemK OS=Methanomassiliicoccales archaeon RumEn M2 OX=1713725 GN=AOA81_03555 PE=4 SV=1
MM1 pKa = 7.2GVADD5 pKa = 3.6ITLTAVWSLPAIPNAEE21 pKa = 4.06EE22 pKa = 4.14TVEE25 pKa = 4.1VAYY28 pKa = 11.0SSVNFSEE35 pKa = 4.35NHH37 pKa = 4.96VEE39 pKa = 3.73PDD41 pKa = 3.1KK42 pKa = 11.04EE43 pKa = 4.24YY44 pKa = 11.41SFGLEE49 pKa = 3.84NGVMARR55 pKa = 11.84FSGNDD60 pKa = 2.76ITGDD64 pKa = 3.54VTFTAISWDD73 pKa = 3.95GEE75 pKa = 4.12FHH77 pKa = 7.31VYY79 pKa = 10.04GADD82 pKa = 3.36VYY84 pKa = 11.04SIDD87 pKa = 3.78MVGSGSVNILIPVDD101 pKa = 3.52KK102 pKa = 10.5FGSPVVHH109 pKa = 7.17HH110 pKa = 7.22IDD112 pKa = 4.45AEE114 pKa = 4.57DD115 pKa = 3.82NDD117 pKa = 4.24VVLTSTIVEE126 pKa = 4.08IGGVKK131 pKa = 9.91YY132 pKa = 10.96ASFDD136 pKa = 3.55ADD138 pKa = 3.63NFSLFYY144 pKa = 10.76VVEE147 pKa = 4.98DD148 pKa = 4.27GSTEE152 pKa = 4.02GGPAGTNTNIMVGVIAIAIAAILVAVWAYY181 pKa = 10.31RR182 pKa = 11.84RR183 pKa = 11.84NALL186 pKa = 4.18

Molecular weight:
19.79 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0Q4B072|A0A0Q4B072_9ARCH Uncharacterized protein OS=Methanomassiliicoccales archaeon RumEn M2 OX=1713725 GN=AOA81_06335 PE=4 SV=1
MM1 pKa = 7.57TNNKK5 pKa = 8.62PPAMKK10 pKa = 10.42ARR12 pKa = 11.84LNKK15 pKa = 10.06AGKK18 pKa = 7.8QNRR21 pKa = 11.84RR22 pKa = 11.84VPAWVMMRR30 pKa = 11.84TNRR33 pKa = 11.84QFLRR37 pKa = 11.84HH38 pKa = 5.85PKK40 pKa = 8.66RR41 pKa = 11.84RR42 pKa = 11.84SWRR45 pKa = 11.84MTSLKK50 pKa = 10.34EE51 pKa = 3.78

Molecular weight:
6.21 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1175

0

1175

312200

39

1188

265.7

29.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.131 ± 0.078

1.489 ± 0.037

6.405 ± 0.052

7.026 ± 0.065

3.716 ± 0.045

8.177 ± 0.062

1.627 ± 0.03

6.97 ± 0.049

5.65 ± 0.075

8.423 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.65 ± 0.041

3.294 ± 0.037

4.024 ± 0.038

1.901 ± 0.03

5.913 ± 0.066

6.84 ± 0.07

5.119 ± 0.063

7.607 ± 0.054

0.889 ± 0.023

3.149 ± 0.045

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski