Freshwater phage uvFW-CGR-AMD-COM-C403

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0XTV7|A0A1B0XTV7_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C403 OX=1838152 GN=uvFWCGRAMDCOMC403_058 PE=4 SV=1
MM1 pKa = 7.79WFTDD5 pKa = 4.09SFCAQCGSPMTTPTPVDD22 pKa = 3.21HH23 pKa = 7.53PDD25 pKa = 3.25YY26 pKa = 10.94VKK28 pKa = 10.59PEE30 pKa = 4.04RR31 pKa = 11.84PEE33 pKa = 4.25EE34 pKa = 4.09EE35 pKa = 4.41DD36 pKa = 5.34DD37 pKa = 3.94EE38 pKa = 5.28FNN40 pKa = 4.32

Molecular weight: 4.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0XTS0|A0A1B0XTS0_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C403 OX=1838152 GN=uvFWCGRAMDCOMC403_014 PE=4 SV=1
MM1 pKa = 8.27DD2 pKa = 5.46PRR4 pKa = 11.84LKK6 pKa = 10.53RR7 pKa = 11.84AGVSGFNKK15 pKa = 9.84PKK17 pKa = 9.6ATPSHH22 pKa = 6.13AKK24 pKa = 9.55KK25 pKa = 10.12SHH27 pKa = 4.84VVVAKK32 pKa = 10.5SGSQVKK38 pKa = 8.89TIRR41 pKa = 11.84FGQQGVSGSPEE52 pKa = 3.68KK53 pKa = 10.87AGEE56 pKa = 4.26TKK58 pKa = 10.32SYY60 pKa = 9.45RR61 pKa = 11.84QRR63 pKa = 11.84RR64 pKa = 11.84QSFKK68 pKa = 10.86ARR70 pKa = 11.84HH71 pKa = 5.87AKK73 pKa = 10.23NISKK77 pKa = 10.67GVMSAAYY84 pKa = 8.79WADD87 pKa = 3.2KK88 pKa = 10.73VKK90 pKa = 9.86WW91 pKa = 3.5

Molecular weight: 10.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11794

40

1645

199.9

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.506 ± 0.831

0.56 ± 0.157

5.901 ± 0.316

5.859 ± 0.409

2.951 ± 0.198

8.267 ± 0.911

0.967 ± 0.185

5.02 ± 0.261

6.444 ± 0.665

7.037 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.174

4.511 ± 0.229

4.095 ± 0.269

3.697 ± 0.264

4.672 ± 0.373

7.283 ± 0.592

7.343 ± 0.626

6.419 ± 0.257

1.569 ± 0.178

3.553 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski