Pectobacterium phage MA13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; unclassified Melnykvirinae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F5J8|A0A5Q2F5J8_9CAUD Tail fiber protein OS=Pectobacterium phage MA13 OX=2662284 GN=MA13_gp27 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84NVIDD6 pKa = 3.72VNPYY10 pKa = 9.63IRR12 pKa = 11.84TFLASEE18 pKa = 4.73LGDD21 pKa = 3.55QIARR25 pKa = 11.84GLFKK29 pKa = 10.54QVGVIRR35 pKa = 11.84TEE37 pKa = 3.98YY38 pKa = 9.16VQQDD42 pKa = 3.5GTTTRR47 pKa = 11.84SMEE50 pKa = 4.01AVIHH54 pKa = 6.65DD55 pKa = 3.89LHH57 pKa = 7.87LDD59 pKa = 3.03KK60 pKa = 11.06LYY62 pKa = 11.0AVEE65 pKa = 4.18QYY67 pKa = 10.59RR68 pKa = 11.84GLAIRR73 pKa = 11.84AEE75 pKa = 4.35VVDD78 pKa = 4.69EE79 pKa = 4.24EE80 pKa = 4.66MLAQMRR86 pKa = 11.84EE87 pKa = 4.14VLPTLAEE94 pKa = 4.51GEE96 pKa = 4.48GFTYY100 pKa = 10.04DD101 pKa = 3.26QRR103 pKa = 11.84IVQDD107 pKa = 3.93VVDD110 pKa = 4.12VAPVDD115 pKa = 3.65EE116 pKa = 4.72SRR118 pKa = 11.84ILAQPLTGEE127 pKa = 4.25EE128 pKa = 4.5GLNGG132 pKa = 3.63

Molecular weight: 14.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F5I7|A0A5Q2F5I7_9CAUD Uncharacterized protein OS=Pectobacterium phage MA13 OX=2662284 GN=MA13_gp17 PE=4 SV=1
MM1 pKa = 7.37GLDD4 pKa = 3.22GCGYY8 pKa = 8.53SRR10 pKa = 11.84KK11 pKa = 9.22PVRR14 pKa = 11.84CYY16 pKa = 10.12PWTVDD21 pKa = 3.0VGGGGRR27 pKa = 11.84VNQVVYY33 pKa = 10.35VAAPAQHH40 pKa = 5.92VDD42 pKa = 2.82ARR44 pKa = 11.84TVRR47 pKa = 11.84AAAARR52 pKa = 11.84TKK54 pKa = 10.65GKK56 pKa = 9.05WEE58 pKa = 3.82SVQRR62 pKa = 11.84VTKK65 pKa = 9.96EE66 pKa = 3.33RR67 pKa = 11.84HH68 pKa = 3.68QQARR72 pKa = 11.84SALRR76 pKa = 11.84MHH78 pKa = 6.75YY79 pKa = 9.79EE80 pKa = 4.07SKK82 pKa = 11.28ANALGMTLAGYY93 pKa = 7.32CQRR96 pKa = 11.84FGVKK100 pKa = 9.62LL101 pKa = 3.48

Molecular weight: 11.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

11317

88

1260

342.9

38.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.861 ± 0.573

0.848 ± 0.133

6.459 ± 0.138

6.106 ± 0.307

3.499 ± 0.208

7.917 ± 0.356

1.829 ± 0.173

4.144 ± 0.259

6.185 ± 0.286

8.43 ± 0.416

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.243 ± 0.222

4.065 ± 0.226

4.277 ± 0.23

4.754 ± 0.464

5.505 ± 0.259

5.814 ± 0.256

5.434 ± 0.353

6.68 ± 0.366

1.396 ± 0.145

3.552 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski