Thermobrachium celere DSM 8682

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Thermobrachium; Thermobrachium celere

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2370 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7RTN9|R7RTN9_9CLOT Spore germination protein gerKC OS=Thermobrachium celere DSM 8682 OX=941824 GN=TCEL_00875 PE=3 SV=1
MM1 pKa = 7.38EE2 pKa = 6.22LDD4 pKa = 3.47AKK6 pKa = 11.11ALVLMKK12 pKa = 10.39RR13 pKa = 11.84EE14 pKa = 3.92NGVLQEE20 pKa = 4.16EE21 pKa = 4.49LGSYY25 pKa = 10.26EE26 pKa = 3.63IDD28 pKa = 3.05EE29 pKa = 5.44GINLVYY35 pKa = 10.2KK36 pKa = 10.67AYY38 pKa = 10.37VEE40 pKa = 3.95EE41 pKa = 4.69DD42 pKa = 3.04KK43 pKa = 11.42VYY45 pKa = 11.02LYY47 pKa = 9.56LTTKK51 pKa = 10.9DD52 pKa = 3.7DD53 pKa = 3.73VSDD56 pKa = 3.87EE57 pKa = 4.59EE58 pKa = 4.23FTQIYY63 pKa = 10.98DD64 pKa = 3.51MYY66 pKa = 11.22DD67 pKa = 3.48LDD69 pKa = 5.37KK70 pKa = 9.68LTQMGYY76 pKa = 9.13EE77 pKa = 4.03VEE79 pKa = 4.45EE80 pKa = 4.39VDD82 pKa = 5.86DD83 pKa = 5.05EE84 pKa = 4.8YY85 pKa = 11.91NPMWCVILDD94 pKa = 4.45FIDD97 pKa = 3.75DD98 pKa = 4.1HH99 pKa = 8.87EE100 pKa = 4.4EE101 pKa = 3.57MEE103 pKa = 4.12ARR105 pKa = 11.84LNEE108 pKa = 4.33VISYY112 pKa = 10.19HH113 pKa = 5.6NSEE116 pKa = 3.83INRR119 pKa = 11.84IYY121 pKa = 11.07SEE123 pKa = 4.06LRR125 pKa = 3.25

Molecular weight: 14.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7RS35|R7RS35_9CLOT DNA replication protein dnaD OS=Thermobrachium celere DSM 8682 OX=941824 GN=TCEL_02070 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.46KK9 pKa = 8.28RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.79RR21 pKa = 11.84MKK23 pKa = 8.66TKK25 pKa = 10.25NGRR28 pKa = 11.84KK29 pKa = 8.24VLQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.92GRR39 pKa = 11.84KK40 pKa = 9.02RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2370

0

2370

709785

37

2827

299.5

33.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.879 ± 0.056

1.101 ± 0.019

5.499 ± 0.041

7.48 ± 0.055

4.456 ± 0.04

6.424 ± 0.05

1.311 ± 0.02

10.112 ± 0.061

8.991 ± 0.043

9.173 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.022

5.752 ± 0.045

2.968 ± 0.033

2.331 ± 0.022

3.889 ± 0.041

5.427 ± 0.042

4.7 ± 0.043

7.113 ± 0.052

0.585 ± 0.014

4.317 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski