Coniosporium apollinis (strain CBS 100218) (Rock-inhabiting black yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetes incertae sedis; Coniosporium; Coniosporium apollinis

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9306 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7YZP4|R7YZP4_CONA1 Uncharacterized protein OS=Coniosporium apollinis (strain CBS 100218) OX=1168221 GN=W97_06517 PE=4 SV=1
MM1 pKa = 7.33ILAPVFLILSTTTLISAQYY20 pKa = 8.85TGYY23 pKa = 11.01SLTSSGDD30 pKa = 3.49PGSVVYY36 pKa = 9.4STSSTPANVSADD48 pKa = 3.58PAPDD52 pKa = 3.17VFLNATVHH60 pKa = 5.69VGEE63 pKa = 4.69IDD65 pKa = 3.17ISVSNLTAKK74 pKa = 10.69LNLDD78 pKa = 3.6AQVLQLLSFNAGVDD92 pKa = 3.57LSIDD96 pKa = 3.55RR97 pKa = 11.84VSLLLADD104 pKa = 4.04VDD106 pKa = 4.22ASVQLEE112 pKa = 4.05VRR114 pKa = 11.84LEE116 pKa = 3.93NLVRR120 pKa = 11.84MINNTLDD127 pKa = 3.83SLDD130 pKa = 4.67LNPILATIGQTVGDD144 pKa = 4.36IANSTLGGLTGGSSPLLAARR164 pKa = 11.84SYY166 pKa = 10.69EE167 pKa = 3.92LEE169 pKa = 3.88NNILYY174 pKa = 10.42SVNDD178 pKa = 3.59YY179 pKa = 11.33SGNTHH184 pKa = 6.12TNRR187 pKa = 11.84ILAQDD192 pKa = 3.84GSIVDD197 pKa = 3.58QSLDD201 pKa = 3.33NDD203 pKa = 3.82GNVYY207 pKa = 9.41NQQVVGSYY215 pKa = 9.67EE216 pKa = 4.26RR217 pKa = 11.84DD218 pKa = 3.2MAFVEE223 pKa = 4.34EE224 pKa = 4.46NEE226 pKa = 4.3GKK228 pKa = 10.08VVNGQAVRR236 pKa = 11.84EE237 pKa = 4.11LEE239 pKa = 4.14YY240 pKa = 10.93VYY242 pKa = 10.84TPFDD246 pKa = 3.67GLSAVCAIYY255 pKa = 10.91VDD257 pKa = 3.64VAGAVVATQVLSEE270 pKa = 4.14SRR272 pKa = 11.84GGGTSTVGADD282 pKa = 3.28LL283 pKa = 4.52

Molecular weight: 29.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7YW04|R7YW04_CONA1 Uncharacterized protein OS=Coniosporium apollinis (strain CBS 100218) OX=1168221 GN=W97_05237 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9306

0

9306

4486276

43

8951

482.1

53.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.349 ± 0.023

1.112 ± 0.01

5.621 ± 0.017

6.509 ± 0.027

3.532 ± 0.017

7.097 ± 0.024

2.331 ± 0.012

4.498 ± 0.017

4.764 ± 0.021

8.797 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.01

3.383 ± 0.013

6.306 ± 0.031

3.973 ± 0.02

6.521 ± 0.021

7.987 ± 0.026

5.886 ± 0.017

6.154 ± 0.018

1.402 ± 0.009

2.68 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski