Eubacterium sp. CAG:841

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1649 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7FW95|R7FW95_9FIRM V-type ATP synthase alpha chain OS=Eubacterium sp. CAG:841 OX=1262894 GN=atpA PE=3 SV=1
MM1 pKa = 7.36IKK3 pKa = 10.43VVFYY7 pKa = 10.79KK8 pKa = 11.1SNGNYY13 pKa = 10.02YY14 pKa = 9.88GFRR17 pKa = 11.84EE18 pKa = 4.47SGHH21 pKa = 6.03SGYY24 pKa = 10.97DD25 pKa = 3.29EE26 pKa = 4.37AGKK29 pKa = 10.29DD30 pKa = 3.38IVCAAVSAMTMLVINTIEE48 pKa = 4.23VAWASDD54 pKa = 3.2VDD56 pKa = 3.79YY57 pKa = 10.53TIDD60 pKa = 5.13DD61 pKa = 3.77KK62 pKa = 10.6TTDD65 pKa = 3.17VTVIARR71 pKa = 11.84GALPEE76 pKa = 4.08YY77 pKa = 10.73EE78 pKa = 3.98EE79 pKa = 6.33DD80 pKa = 3.39EE81 pKa = 4.5AKK83 pKa = 10.57RR84 pKa = 11.84FAISGLIYY92 pKa = 10.41SYY94 pKa = 10.93FLQLNDD100 pKa = 4.47MIEE103 pKa = 4.2DD104 pKa = 3.94YY105 pKa = 11.6YY106 pKa = 11.7DD107 pKa = 4.44FIEE110 pKa = 4.18VDD112 pKa = 3.72EE113 pKa = 4.44EE114 pKa = 4.1EE115 pKa = 5.05KK116 pKa = 10.68III118 pKa = 4.82

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7FQZ5|R7FQZ5_9FIRM Uncharacterized protein OS=Eubacterium sp. CAG:841 OX=1262894 GN=BN797_01165 PE=4 SV=1
MM1 pKa = 6.44YY2 pKa = 9.38TSAKK6 pKa = 9.6PYY8 pKa = 10.0FYY10 pKa = 10.51GTGRR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 10.23KK17 pKa = 9.04SVARR21 pKa = 11.84VRR23 pKa = 11.84LYY25 pKa = 10.37PGTGTVTINGRR36 pKa = 11.84DD37 pKa = 3.18IDD39 pKa = 4.72DD40 pKa = 3.89YY41 pKa = 11.71FGLEE45 pKa = 4.0TLKK48 pKa = 11.21LIVNQPFAVTSTQGKK63 pKa = 9.54FDD65 pKa = 4.04VVCTAAGGGISGQAGAIRR83 pKa = 11.84HH84 pKa = 5.86GISRR88 pKa = 11.84ALLLADD94 pKa = 3.82DD95 pKa = 5.08SYY97 pKa = 11.37RR98 pKa = 11.84TLLKK102 pKa = 10.66KK103 pKa = 10.68EE104 pKa = 4.08GLLTRR109 pKa = 11.84DD110 pKa = 3.18PRR112 pKa = 11.84MKK114 pKa = 10.18EE115 pKa = 3.54RR116 pKa = 11.84KK117 pKa = 9.62KK118 pKa = 11.05YY119 pKa = 9.44GLKK122 pKa = 9.61AARR125 pKa = 11.84RR126 pKa = 11.84ASQFSKK132 pKa = 10.85RR133 pKa = 3.65

Molecular weight: 14.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1649

0

1649

518552

30

1881

314.5

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.295 ± 0.067

1.859 ± 0.03

5.872 ± 0.046

7.058 ± 0.062

4.364 ± 0.045

7.35 ± 0.05

1.499 ± 0.025

7.499 ± 0.05

7.166 ± 0.045

8.421 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.026

3.938 ± 0.042

3.355 ± 0.031

2.02 ± 0.029

4.446 ± 0.049

6.807 ± 0.05

5.788 ± 0.058

6.756 ± 0.049

0.752 ± 0.019

4.061 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski