Nitrincola sp. A-D6

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Nitrincola; unclassified Nitrincola

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2517 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A099PEH3|A0A099PEH3_9GAMM HTH gntR-type domain-containing protein OS=Nitrincola sp. A-D6 OX=1545442 GN=LH51_00580 PE=4 SV=1
MM1 pKa = 7.67NDD3 pKa = 2.89ISLQADD9 pKa = 3.43SAYY12 pKa = 10.22EE13 pKa = 3.62KK14 pKa = 10.64LIRR17 pKa = 11.84LEE19 pKa = 4.47ASAEE23 pKa = 4.12TSSDD27 pKa = 3.42EE28 pKa = 4.65LFCCAYY34 pKa = 10.9LLGHH38 pKa = 7.28LSLINGQEE46 pKa = 4.35SMDD49 pKa = 3.96STSLDD54 pKa = 3.55QLMHH58 pKa = 6.68TSLQQAFSVDD68 pKa = 3.23HH69 pKa = 6.59LTDD72 pKa = 3.19QDD74 pKa = 3.43KK75 pKa = 10.93AAIMALWDD83 pKa = 4.14SLCLSADD90 pKa = 3.1QDD92 pKa = 3.65

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A099PEJ4|A0A099PEJ4_9GAMM Uncharacterized protein OS=Nitrincola sp. A-D6 OX=1545442 GN=LH51_07010 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.47NGSQVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.64GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2517

0

2517

686487

33

1407

272.7

30.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.353 ± 0.052

1.051 ± 0.016

5.609 ± 0.038

6.172 ± 0.047

3.814 ± 0.033

7.029 ± 0.045

2.294 ± 0.025

5.805 ± 0.038

3.838 ± 0.035

11.341 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.026

3.523 ± 0.036

4.347 ± 0.034

4.958 ± 0.045

5.77 ± 0.046

6.353 ± 0.042

5.214 ± 0.04

6.836 ± 0.044

1.297 ± 0.02

2.751 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski