Trichinella sp. T8 
Average proteome isoelectric point is 7.2 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 18039 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A0V1LXF6|A0A0V1LXF6_9BILA Uncharacterized protein OS=Trichinella sp. T8 OX=92180 GN=T08_16602 PE=4 SV=1 
MM1 pKa = 7.59  LLCVYY6 pKa = 10.54  YY7 pKa = 10.54  SFKK10 pKa = 10.95  LSGISSPEE18 pKa = 3.77  DD19 pKa = 3.08  VTTEE23 pKa = 3.57  KK24 pKa = 11.15  DD25 pKa = 3.52  EE26 pKa = 5.68  NIVCLNEE33 pKa = 4.37  LVTLHH38 pKa = 6.05  TAVSEE43 pKa = 4.19  RR44 pKa = 11.84  RR45 pKa = 11.84  LITDD49 pKa = 5.16  DD50 pKa = 4.06  IINVQDD56 pKa = 3.45  YY57 pKa = 10.98  AAIDD61 pKa = 3.9  DD62 pKa = 4.04  EE63 pKa = 5.66  ANVHH67 pKa = 6.05  EE68 pKa = 4.85  CTEE71 pKa = 4.2  GTADD75 pKa = 4.13  EE76 pKa = 4.39  IVEE79 pKa = 4.23  EE80 pKa = 4.36  LLSS83 pKa = 3.77   
 Molecular weight: 9.26 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.76 
IPC2_protein 3.91 
IPC_protein 3.834 
Toseland    3.643 
ProMoST     3.973 
Dawson      3.795 
Bjellqvist  3.961 
Wikipedia   3.706 
Rodwell     3.668 
Grimsley    3.567 
Solomon     3.783 
Lehninger   3.732 
Nozaki      3.923 
DTASelect   4.075 
Thurlkill   3.694 
EMBOSS      3.719 
Sillero     3.948 
Patrickios  0.846 
IPC_peptide 3.783 
IPC2_peptide  3.923 
IPC2.peptide.svr19  3.833 
 Protein with the highest isoelectric point: 
>tr|A0A0V1LYV8|A0A0V1LYV8_9BILA Uncharacterized protein (Fragment) OS=Trichinella sp. T8 OX=92180 GN=T08_6325 PE=4 SV=1 
MM1 pKa = 7.32  ARR3 pKa = 11.84  SARR6 pKa = 11.84  SSSRR10 pKa = 11.84  SRR12 pKa = 11.84  SRR14 pKa = 11.84  SSTRR18 pKa = 11.84  RR19 pKa = 11.84  SMSSRR24 pKa = 11.84  RR25 pKa = 11.84  GSLTRR30 pKa = 11.84  PRR32 pKa = 11.84  RR33 pKa = 11.84  RR34 pKa = 11.84  PISRR38 pKa = 11.84  IQTPKK43 pKa = 10.33  RR44 pKa = 11.84  STRR47 pKa = 11.84  QILL50 pKa = 3.35   
 Molecular weight: 5.84 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.52 
IPC2_protein 11.403 
IPC_protein 13.013 
Toseland    13.173 
ProMoST     13.671 
Dawson      13.173 
Bjellqvist  13.173 
Wikipedia   13.656 
Rodwell     12.705 
Grimsley    13.217 
Solomon     13.671 
Lehninger   13.583 
Nozaki      13.173 
DTASelect   13.173 
Thurlkill   13.173 
EMBOSS      13.671 
Sillero     13.173 
Patrickios  12.427 
IPC_peptide 13.685 
IPC2_peptide  12.676 
IPC2.peptide.svr19  9.316 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        14641 
 
        
        3398 
 
        
        18039 
         
        8199004
 
        30
 
        7525
 
        454.5
 
        51.44
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        6.442 ± 0.016
2.621 ± 0.018
 
        5.212 ± 0.013
6.304 ± 0.023
 
        4.576 ± 0.014
4.978 ± 0.017
 
        2.478 ± 0.008
5.551 ± 0.015
       
        5.998 ± 0.018
9.693 ± 0.022
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.418 ± 0.007
4.989 ± 0.013
 
        4.345 ± 0.016
4.287 ± 0.016
 
        5.691 ± 0.015
8.366 ± 0.019
 
        5.312 ± 0.013
6.416 ± 0.013
       
        1.221 ± 0.007
3.097 ± 0.011
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here