Amino acid dipepetide frequency for Pseudomonas salegens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.951AlaAla: 11.951 ± 0.141	1.254AlaCys: 1.254 ± 0.037	6.901AlaAsp: 6.901 ± 0.095	7.438AlaGlu: 7.438 ± 0.109	3.459AlaPhe: 3.459 ± 0.068	8.721AlaGly: 8.721 ± 0.1	2.138AlaHis: 2.138 ± 0.043	5.279AlaIle: 5.279 ± 0.073	2.769AlaLys: 2.769 ± 0.053	12.889AlaLeu: 12.889 ± 0.124	2.858AlaMet: 2.858 ± 0.061	3.025AlaAsn: 3.025 ± 0.059	4.041AlaPro: 4.041 ± 0.068	4.386AlaGln: 4.386 ± 0.072	6.76AlaArg: 6.76 ± 0.088	6.13AlaSer: 6.13 ± 0.076	4.686AlaThr: 4.686 ± 0.067	7.096AlaVal: 7.096 ± 0.08	1.561AlaTrp: 1.561 ± 0.039	2.298AlaTyr: 2.298 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	1.039CysAla: 1.039 ± 0.034	0.149CysCys: 0.149 ± 0.011	0.556CysAsp: 0.556 ± 0.024	0.538CysGlu: 0.538 ± 0.025	0.36CysPhe: 0.36 ± 0.017	0.934CysGly: 0.934 ± 0.029	0.306CysHis: 0.306 ± 0.019	0.506CysIle: 0.506 ± 0.022	0.298CysLys: 0.298 ± 0.014	1.183CysLeu: 1.183 ± 0.034	0.231CysMet: 0.231 ± 0.016	0.328CysAsn: 0.328 ± 0.016	0.545CysPro: 0.545 ± 0.026	0.493CysGln: 0.493 ± 0.024	0.707CysArg: 0.707 ± 0.027	0.655CysSer: 0.655 ± 0.028	0.482CysThr: 0.482 ± 0.022	0.648CysVal: 0.648 ± 0.021	0.165CysTrp: 0.165 ± 0.012	0.254CysTyr: 0.254 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	5.624AspAla: 5.624 ± 0.082	0.636AspCys: 0.636 ± 0.026	3.396AspAsp: 3.396 ± 0.064	3.589AspGlu: 3.589 ± 0.058	2.24AspPhe: 2.24 ± 0.048	4.374AspGly: 4.374 ± 0.07	1.191AspHis: 1.191 ± 0.032	3.13AspIle: 3.13 ± 0.057	1.766AspLys: 1.766 ± 0.045	6.156AspLeu: 6.156 ± 0.075	1.518AspMet: 1.518 ± 0.038	2.048AspAsn: 2.048 ± 0.051	2.891AspPro: 2.891 ± 0.053	2.795AspGln: 2.795 ± 0.054	3.274AspArg: 3.274 ± 0.058	3.496AspSer: 3.496 ± 0.062	2.79AspThr: 2.79 ± 0.061	3.637AspVal: 3.637 ± 0.062	1.18AspTrp: 1.18 ± 0.037	1.921AspTyr: 1.921 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.111GluAla: 6.111 ± 0.077	0.439GluCys: 0.439 ± 0.02	2.733GluAsp: 2.733 ± 0.051	3.214GluGlu: 3.214 ± 0.069	1.8GluPhe: 1.8 ± 0.044	3.7GluGly: 3.7 ± 0.06	1.73GluHis: 1.73 ± 0.04	2.718GluIle: 2.718 ± 0.053	1.972GluLys: 1.972 ± 0.047	7.319GluLeu: 7.319 ± 0.085	1.491GluMet: 1.491 ± 0.031	1.533GluAsn: 1.533 ± 0.039	2.753GluPro: 2.753 ± 0.065	4.534GluGln: 4.534 ± 0.08	4.895GluArg: 4.895 ± 0.069	2.907GluSer: 2.907 ± 0.049	2.638GluThr: 2.638 ± 0.059	4.02GluVal: 4.02 ± 0.068	0.772GluTrp: 0.772 ± 0.027	1.409GluTyr: 1.409 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.017PheAla: 4.017 ± 0.073	0.447PheCys: 0.447 ± 0.021	2.284PheAsp: 2.284 ± 0.05	1.927PheGlu: 1.927 ± 0.038	1.483PhePhe: 1.483 ± 0.038	2.922PheGly: 2.922 ± 0.055	0.727PheHis: 0.727 ± 0.026	1.876PheIle: 1.876 ± 0.047	1.061PheLys: 1.061 ± 0.033	3.293PheLeu: 3.293 ± 0.051	0.94PheMet: 0.94 ± 0.033	1.379PheAsn: 1.379 ± 0.04	1.441PhePro: 1.441 ± 0.037	1.169PheGln: 1.169 ± 0.034	1.915PheArg: 1.915 ± 0.041	2.619PheSer: 2.619 ± 0.047	1.957PheThr: 1.957 ± 0.042	2.455PheVal: 2.455 ± 0.061	0.54PheTrp: 0.54 ± 0.024	1.095PheTyr: 1.095 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	6.493GlyAla: 6.493 ± 0.083	0.987GlyCys: 0.987 ± 0.027	3.972GlyAsp: 3.972 ± 0.064	4.632GlyGlu: 4.632 ± 0.066	3.279GlyPhe: 3.279 ± 0.06	5.697GlyGly: 5.697 ± 0.106	1.931GlyHis: 1.931 ± 0.048	4.247GlyIle: 4.247 ± 0.063	2.952GlyLys: 2.952 ± 0.061	8.9GlyLeu: 8.9 ± 0.104	2.316GlyMet: 2.316 ± 0.045	2.58GlyAsn: 2.58 ± 0.054	2.602GlyPro: 2.602 ± 0.054	3.718GlyGln: 3.718 ± 0.057	4.758GlyArg: 4.758 ± 0.079	4.706GlySer: 4.706 ± 0.085	3.589GlyThr: 3.589 ± 0.07	5.507GlyVal: 5.507 ± 0.075	1.346GlyTrp: 1.346 ± 0.034	2.407GlyTyr: 2.407 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.383HisAla: 2.383 ± 0.048	0.378HisCys: 0.378 ± 0.021	1.279HisAsp: 1.279 ± 0.036	1.183HisGlu: 1.183 ± 0.035	1.014HisPhe: 1.014 ± 0.031	1.859HisGly: 1.859 ± 0.046	0.631HisHis: 0.631 ± 0.022	1.152HisIle: 1.152 ± 0.028	0.658HisLys: 0.658 ± 0.025	2.612HisLeu: 2.612 ± 0.054	0.538HisMet: 0.538 ± 0.025	0.752HisAsn: 0.752 ± 0.025	1.482HisPro: 1.482 ± 0.038	1.145HisGln: 1.145 ± 0.037	1.348HisArg: 1.348 ± 0.036	1.434HisSer: 1.434 ± 0.036	1.066HisThr: 1.066 ± 0.033	1.247HisVal: 1.247 ± 0.036	0.454HisTrp: 0.454 ± 0.023	0.872HisTyr: 0.872 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.66IleAla: 5.66 ± 0.075	0.512IleCys: 0.512 ± 0.023	3.397IleAsp: 3.397 ± 0.063	3.493IleGlu: 3.493 ± 0.072	1.517IlePhe: 1.517 ± 0.04	4.32IleGly: 4.32 ± 0.073	1.094IleHis: 1.094 ± 0.033	2.43IleIle: 2.43 ± 0.049	1.655IleLys: 1.655 ± 0.042	4.389IleLeu: 4.389 ± 0.062	1.035IleMet: 1.035 ± 0.032	2.062IleAsn: 2.062 ± 0.05	2.335IlePro: 2.335 ± 0.046	1.766IleGln: 1.766 ± 0.038	3.004IleArg: 3.004 ± 0.052	3.305IleSer: 3.305 ± 0.054	2.702IleThr: 2.702 ± 0.05	3.05IleVal: 3.05 ± 0.053	0.58IleTrp: 0.58 ± 0.023	1.168IleTyr: 1.168 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.41LysAla: 3.41 ± 0.076	0.186LysCys: 0.186 ± 0.012	1.546LysAsp: 1.546 ± 0.037	1.483LysGlu: 1.483 ± 0.039	0.67LysPhe: 0.67 ± 0.03	2.182LysGly: 2.182 ± 0.052	0.712LysHis: 0.712 ± 0.023	1.372LysIle: 1.372 ± 0.039	1.165LysLys: 1.165 ± 0.045	3.07LysLeu: 3.07 ± 0.054	0.662LysMet: 0.662 ± 0.025	0.862LysAsn: 0.862 ± 0.028	1.817LysPro: 1.817 ± 0.04	1.514LysGln: 1.514 ± 0.038	2.214LysArg: 2.214 ± 0.047	1.606LysSer: 1.606 ± 0.038	1.672LysThr: 1.672 ± 0.04	2.276LysVal: 2.276 ± 0.05	0.292LysTrp: 0.292 ± 0.016	0.6LysTyr: 0.6 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.601LeuAla: 13.601 ± 0.14	1.233LeuCys: 1.233 ± 0.037	7.141LeuAsp: 7.141 ± 0.084	6.482LeuGlu: 6.482 ± 0.08	4.079LeuPhe: 4.079 ± 0.074	8.666LeuGly: 8.666 ± 0.102	2.687LeuHis: 2.687 ± 0.056	5.703LeuIle: 5.703 ± 0.075	3.481LeuLys: 3.481 ± 0.06	14.288LeuLeu: 14.288 ± 0.183	2.674LeuMet: 2.674 ± 0.053	3.55LeuAsn: 3.55 ± 0.056	6.427LeuPro: 6.427 ± 0.089	5.783LeuGln: 5.783 ± 0.077	7.313LeuArg: 7.313 ± 0.089	7.142LeuSer: 7.142 ± 0.081	5.927LeuThr: 5.927 ± 0.077	7.903LeuVal: 7.903 ± 0.095	1.449LeuTrp: 1.449 ± 0.039	2.449LeuTyr: 2.449 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.807MetAla: 2.807 ± 0.047	0.179MetCys: 0.179 ± 0.012	1.238MetAsp: 1.238 ± 0.034	1.054MetGlu: 1.054 ± 0.031	0.706MetPhe: 0.706 ± 0.028	1.771MetGly: 1.771 ± 0.042	0.582MetHis: 0.582 ± 0.024	1.149MetIle: 1.149 ± 0.03	0.796MetLys: 0.796 ± 0.025	3.049MetLeu: 3.049 ± 0.056	0.517MetMet: 0.517 ± 0.022	0.802MetAsn: 0.802 ± 0.029	1.458MetPro: 1.458 ± 0.035	1.438MetGln: 1.438 ± 0.035	1.659MetArg: 1.659 ± 0.038	1.699MetSer: 1.699 ± 0.04	1.533MetThr: 1.533 ± 0.041	1.653MetVal: 1.653 ± 0.045	0.177MetTrp: 0.177 ± 0.012	0.401MetTyr: 0.401 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.227AsnAla: 3.227 ± 0.066	0.309AsnCys: 0.309 ± 0.017	1.696AsnAsp: 1.696 ± 0.048	1.633AsnGlu: 1.633 ± 0.039	1.054AsnPhe: 1.054 ± 0.033	2.396AsnGly: 2.396 ± 0.049	0.695AsnHis: 0.695 ± 0.028	1.523AsnIle: 1.523 ± 0.04	0.915AsnLys: 0.915 ± 0.028	3.421AsnLeu: 3.421 ± 0.062	0.742AsnMet: 0.742 ± 0.026	1.184AsnAsn: 1.184 ± 0.04	2.074AsnPro: 2.074 ± 0.048	1.605AsnGln: 1.605 ± 0.039	2.067AsnArg: 2.067 ± 0.048	1.686AsnSer: 1.686 ± 0.044	1.692AsnThr: 1.692 ± 0.048	1.847AsnVal: 1.847 ± 0.044	0.508AsnTrp: 0.508 ± 0.023	0.846AsnTyr: 0.846 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	5.535ProAla: 5.535 ± 0.088	0.434ProCys: 0.434 ± 0.031	3.465ProAsp: 3.465 ± 0.058	3.787ProGlu: 3.787 ± 0.068	1.728ProPhe: 1.728 ± 0.042	4.284ProGly: 4.284 ± 0.075	0.977ProHis: 0.977 ± 0.029	2.048ProIle: 2.048 ± 0.042	1.109ProLys: 1.109 ± 0.033	5.56ProLeu: 5.56 ± 0.084	1.174ProMet: 1.174 ± 0.032	1.269ProAsn: 1.269 ± 0.034	2.096ProPro: 2.096 ± 0.057	2.132ProGln: 2.132 ± 0.05	2.506ProArg: 2.506 ± 0.049	2.443ProSer: 2.443 ± 0.041	2.139ProThr: 2.139 ± 0.046	3.892ProVal: 3.892 ± 0.063	0.794ProTrp: 0.794 ± 0.026	1.105ProTyr: 1.105 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	6.41GlnAla: 6.41 ± 0.09	0.394GlnCys: 0.394 ± 0.021	2.068GlnAsp: 2.068 ± 0.045	2.179GlnGlu: 2.179 ± 0.048	1.344GlnPhe: 1.344 ± 0.032	3.625GlnGly: 3.625 ± 0.067	1.442GlnHis: 1.442 ± 0.037	2.193GlnIle: 2.193 ± 0.04	1.012GlnLys: 1.012 ± 0.03	6.217GlnLeu: 6.217 ± 0.097	1.021GlnMet: 1.021 ± 0.026	1.048GlnAsn: 1.048 ± 0.034	2.902GlnPro: 2.902 ± 0.055	3.866GlnGln: 3.866 ± 0.097	4.193GlnArg: 4.193 ± 0.086	2.534GlnSer: 2.534 ± 0.049	2.115GlnThr: 2.115 ± 0.046	3.769GlnVal: 3.769 ± 0.056	0.79GlnTrp: 0.79 ± 0.031	0.967GlnTyr: 0.967 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	5.545ArgAla: 5.545 ± 0.083	0.602ArgCys: 0.602 ± 0.025	3.658ArgAsp: 3.658 ± 0.056	4.314ArgGlu: 4.314 ± 0.071	2.74ArgPhe: 2.74 ± 0.051	3.847ArgGly: 3.847 ± 0.053	1.804ArgHis: 1.804 ± 0.044	3.55ArgIle: 3.55 ± 0.056	2.053ArgLys: 2.053 ± 0.05	8.503ArgLeu: 8.503 ± 0.099	1.701ArgMet: 1.701 ± 0.042	2.1ArgAsn: 2.1 ± 0.047	2.73ArgPro: 2.73 ± 0.054	3.955ArgGln: 3.955 ± 0.063	4.243ArgArg: 4.243 ± 0.07	3.541ArgSer: 3.541 ± 0.054	2.616ArgThr: 2.616 ± 0.047	4.396ArgVal: 4.396 ± 0.078	1.164ArgTrp: 1.164 ± 0.034	2.13ArgTyr: 2.13 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	6.017SerAla: 6.017 ± 0.079	0.566SerCys: 0.566 ± 0.025	3.157SerAsp: 3.157 ± 0.046	3.299SerGlu: 3.299 ± 0.061	2.205SerPhe: 2.205 ± 0.048	5.49SerGly: 5.49 ± 0.076	1.3SerHis: 1.3 ± 0.04	2.904SerIle: 2.904 ± 0.055	1.643SerLys: 1.643 ± 0.038	7.038SerLeu: 7.038 ± 0.087	1.488SerMet: 1.488 ± 0.035	1.867SerAsn: 1.867 ± 0.043	2.799SerPro: 2.799 ± 0.057	2.641SerGln: 2.641 ± 0.051	3.821SerArg: 3.821 ± 0.049	3.666SerSer: 3.666 ± 0.074	2.814SerThr: 2.814 ± 0.061	3.96SerVal: 3.96 ± 0.061	0.863SerTrp: 0.863 ± 0.033	1.467SerTyr: 1.467 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	5.108ThrAla: 5.108 ± 0.07	0.495ThrCys: 0.495 ± 0.02	2.967ThrAsp: 2.967 ± 0.059	2.589ThrGlu: 2.589 ± 0.048	1.648ThrPhe: 1.648 ± 0.047	4.273ThrGly: 4.273 ± 0.075	1.085ThrHis: 1.085 ± 0.031	2.045ThrIle: 2.045 ± 0.049	0.975ThrLys: 0.975 ± 0.034	6.764ThrLeu: 6.764 ± 0.079	0.941ThrMet: 0.941 ± 0.028	1.315ThrAsn: 1.315 ± 0.039	2.903ThrPro: 2.903 ± 0.054	1.855ThrGln: 1.855 ± 0.039	3.242ThrArg: 3.242 ± 0.051	2.699ThrSer: 2.699 ± 0.058	2.455ThrThr: 2.455 ± 0.058	3.224ThrVal: 3.224 ± 0.065	0.627ThrTrp: 0.627 ± 0.023	1.109ThrTyr: 1.109 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	7.191ValAla: 7.191 ± 0.085	0.699ValCys: 0.699 ± 0.026	4.168ValAsp: 4.168 ± 0.062	4.085ValGlu: 4.085 ± 0.065	2.608ValPhe: 2.608 ± 0.053	4.744ValGly: 4.744 ± 0.073	1.412ValHis: 1.412 ± 0.039	3.975ValIle: 3.975 ± 0.063	2.045ValLys: 2.045 ± 0.051	7.769ValLeu: 7.769 ± 0.096	1.921ValMet: 1.921 ± 0.04	2.29ValAsn: 2.29 ± 0.049	3.067ValPro: 3.067 ± 0.055	2.468ValGln: 2.468 ± 0.047	4.174ValArg: 4.174 ± 0.076	4.431ValSer: 4.431 ± 0.066	3.623ValThr: 3.623 ± 0.07	5.281ValVal: 5.281 ± 0.072	0.847ValTrp: 0.847 ± 0.028	1.576ValTyr: 1.576 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.104TrpAla: 1.104 ± 0.035	0.171TrpCys: 0.171 ± 0.012	0.639TrpAsp: 0.639 ± 0.024	0.64TrpGlu: 0.64 ± 0.027	0.56TrpPhe: 0.56 ± 0.022	0.886TrpGly: 0.886 ± 0.03	0.425TrpHis: 0.425 ± 0.02	0.633TrpIle: 0.633 ± 0.025	0.339TrpLys: 0.339 ± 0.018	2.462TrpLeu: 2.462 ± 0.052	0.381TrpMet: 0.381 ± 0.019	0.419TrpAsn: 0.419 ± 0.021	0.753TrpPro: 0.753 ± 0.028	1.291TrpGln: 1.291 ± 0.04	1.097TrpArg: 1.097 ± 0.036	0.825TrpSer: 0.825 ± 0.028	0.601TrpThr: 0.601 ± 0.024	0.993TrpVal: 0.993 ± 0.027	0.259TrpTrp: 0.259 ± 0.019	0.345TrpTyr: 0.345 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.409TyrAla: 2.409 ± 0.052	0.309TyrCys: 0.309 ± 0.015	1.391TyrAsp: 1.391 ± 0.04	1.131TyrGlu: 1.131 ± 0.039	1.054TyrPhe: 1.054 ± 0.032	1.929TyrGly: 1.929 ± 0.045	0.619TyrHis: 0.619 ± 0.028	1.041TyrIle: 1.041 ± 0.033	0.646TyrLys: 0.646 ± 0.024	3.093TyrLeu: 3.093 ± 0.05	0.516TyrMet: 0.516 ± 0.021	0.703TyrAsn: 0.703 ± 0.026	1.362TyrPro: 1.362 ± 0.034	1.539TyrGln: 1.539 ± 0.039	2.004TyrArg: 2.004 ± 0.043	1.481TyrSer: 1.481 ± 0.038	1.223TyrThr: 1.223 ± 0.035	1.517TyrVal: 1.517 ± 0.04	0.454TyrTrp: 0.454 ± 0.02	0.74TyrTyr: 0.74 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3414 proteins (1136679 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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