Sphaerosporella brunnea

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; Pezizomycetes; Pezizales; Pyronemataceae; Sphaerosporella

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12382 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J5F1A3|A0A5J5F1A3_9PEZI Eukaryotic translation initiation factor 3 subunit E OS=Sphaerosporella brunnea OX=1250544 GN=INT6 PE=3 SV=1
MM1 pKa = 7.23YY2 pKa = 10.68SSATTIAPPTQHH14 pKa = 7.32DD15 pKa = 4.09SHH17 pKa = 6.8TGSDD21 pKa = 3.54TPSGVEE27 pKa = 3.95EE28 pKa = 4.25SHH30 pKa = 7.82VDD32 pKa = 3.52DD33 pKa = 6.21DD34 pKa = 4.66VLIDD38 pKa = 3.65PAVNPDD44 pKa = 3.36CTAISAAEE52 pKa = 3.85QQACVVLSVLFLDD65 pKa = 4.36TEE67 pKa = 4.49VTEE70 pKa = 4.95SDD72 pKa = 3.51ISDD75 pKa = 4.26LAHH78 pKa = 6.24QLHH81 pKa = 6.65GLDD84 pKa = 5.1LEE86 pKa = 4.52NATLRR91 pKa = 11.84NLLCYY96 pKa = 10.56DD97 pKa = 4.78LFPVLWGNIMAAAGEE112 pKa = 4.32WAWFDD117 pKa = 4.58AEE119 pKa = 4.29WLLGAQLRR127 pKa = 11.84VAGAPGRR134 pKa = 11.84AVVV137 pKa = 3.61

Molecular weight: 14.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J5EGN8|A0A5J5EGN8_9PEZI Uncharacterized protein OS=Sphaerosporella brunnea OX=1250544 GN=FN846DRAFT_894980 PE=4 SV=1
LL1 pKa = 7.47KK2 pKa = 10.31LRR4 pKa = 11.84SPQTSFSSNFVLLKK18 pKa = 10.49LRR20 pKa = 11.84SPQTSFSSNFVLLKK34 pKa = 10.62LRR36 pKa = 11.84STQTSFSSNFVLLKK50 pKa = 10.49LRR52 pKa = 11.84SPQTSFSSNFVLLKK66 pKa = 10.49LRR68 pKa = 11.84SPQTSFSSNFVLLKK82 pKa = 10.49LRR84 pKa = 11.84SPQTSFSSNFVLLKK98 pKa = 10.49LRR100 pKa = 11.84SPQTSFSSNFVLLKK114 pKa = 10.6LRR116 pKa = 11.84SPQQ119 pKa = 3.16

Molecular weight: 13.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12382

0

12382

4913285

49

6584

396.8

43.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.038 ± 0.025

1.396 ± 0.012

5.348 ± 0.017

6.572 ± 0.026

3.59 ± 0.013

6.8 ± 0.021

2.441 ± 0.011

4.545 ± 0.015

5.049 ± 0.025

8.87 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.008

3.374 ± 0.013

6.437 ± 0.029

3.835 ± 0.018

6.61 ± 0.023

7.726 ± 0.024

5.922 ± 0.017

6.271 ± 0.017

1.438 ± 0.008

2.55 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski