Amino acid dipepetide frequency for Staphylococcus virus phiETA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.521AlaAla: 0.521 ± 0.212
0.149AlaCys: 0.149 ± 0.092
2.235AlaAsp: 2.235 ± 0.41
3.278AlaGlu: 3.278 ± 0.387
2.905AlaPhe: 2.905 ± 0.546
3.725AlaGly: 3.725 ± 0.816
0.968AlaHis: 0.968 ± 0.273
5.438AlaIle: 5.438 ± 0.638
6.63AlaLys: 6.63 ± 0.75
4.768AlaLeu: 4.768 ± 0.642
1.266AlaMet: 1.266 ± 0.459
3.725AlaAsn: 3.725 ± 0.442
2.086AlaPro: 2.086 ± 0.37
2.682AlaGln: 2.682 ± 0.428
2.831AlaArg: 2.831 ± 0.411
4.321AlaSer: 4.321 ± 0.659
3.576AlaThr: 3.576 ± 0.509
3.948AlaVal: 3.948 ± 0.733
0.968AlaTrp: 0.968 ± 0.335
2.533AlaTyr: 2.533 ± 0.429
0.0AlaXaa: 0.0 ± 0.0
Cys
0.223CysAla: 0.223 ± 0.13
0.0CysCys: 0.0 ± 0.0
0.149CysAsp: 0.149 ± 0.106
0.447CysGlu: 0.447 ± 0.227
0.372CysPhe: 0.372 ± 0.149
0.298CysGly: 0.298 ± 0.142
0.074CysHis: 0.074 ± 0.086
0.074CysIle: 0.074 ± 0.074
0.447CysLys: 0.447 ± 0.152
0.223CysLeu: 0.223 ± 0.133
0.149CysMet: 0.149 ± 0.115
0.223CysAsn: 0.223 ± 0.119
0.149CysPro: 0.149 ± 0.096
0.223CysGln: 0.223 ± 0.104
0.372CysArg: 0.372 ± 0.199
0.298CysSer: 0.298 ± 0.173
0.0CysThr: 0.0 ± 0.0
0.372CysVal: 0.372 ± 0.196
0.074CysTrp: 0.074 ± 0.061
0.298CysTyr: 0.298 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
4.246AspAla: 4.246 ± 0.629
0.223AspCys: 0.223 ± 0.112
4.842AspAsp: 4.842 ± 0.789
5.289AspGlu: 5.289 ± 0.734
3.129AspPhe: 3.129 ± 0.548
4.619AspGly: 4.619 ± 0.623
0.372AspHis: 0.372 ± 0.165
4.917AspIle: 4.917 ± 0.794
6.63AspLys: 6.63 ± 0.886
5.14AspLeu: 5.14 ± 0.63
1.639AspMet: 1.639 ± 0.353
2.98AspAsn: 2.98 ± 0.461
0.819AspPro: 0.819 ± 0.234
1.415AspGln: 1.415 ± 0.352
2.235AspArg: 2.235 ± 0.413
4.321AspSer: 4.321 ± 0.558
3.129AspThr: 3.129 ± 0.468
3.948AspVal: 3.948 ± 0.688
0.819AspTrp: 0.819 ± 0.235
2.458AspTyr: 2.458 ± 0.373
0.0AspXaa: 0.0 ± 0.0
Glu
4.246GluAla: 4.246 ± 0.587
0.521GluCys: 0.521 ± 0.175
4.097GluAsp: 4.097 ± 0.672
5.662GluGlu: 5.662 ± 0.903
2.756GluPhe: 2.756 ± 0.597
2.756GluGly: 2.756 ± 0.473
1.862GluHis: 1.862 ± 0.392
5.438GluIle: 5.438 ± 0.657
5.215GluLys: 5.215 ± 0.742
8.641GluLeu: 8.641 ± 1.13
2.011GluMet: 2.011 ± 0.435
4.023GluAsn: 4.023 ± 0.469
1.862GluPro: 1.862 ± 0.367
4.023GluGln: 4.023 ± 0.622
3.129GluArg: 3.129 ± 0.563
3.129GluSer: 3.129 ± 0.479
3.65GluThr: 3.65 ± 0.479
5.215GluVal: 5.215 ± 0.538
1.266GluTrp: 1.266 ± 0.273
3.427GluTyr: 3.427 ± 0.577
0.0GluXaa: 0.0 ± 0.0
Phe
1.713PheAla: 1.713 ± 0.325
0.372PheCys: 0.372 ± 0.161
3.725PheAsp: 3.725 ± 0.436
3.203PheGlu: 3.203 ± 0.597
1.043PhePhe: 1.043 ± 0.271
2.905PheGly: 2.905 ± 0.732
0.819PheHis: 0.819 ± 0.227
3.874PheIle: 3.874 ± 0.561
4.023PheLys: 4.023 ± 0.576
2.905PheLeu: 2.905 ± 0.454
0.819PheMet: 0.819 ± 0.206
2.831PheAsn: 2.831 ± 0.359
0.894PhePro: 0.894 ± 0.272
1.117PheGln: 1.117 ± 0.299
1.341PheArg: 1.341 ± 0.291
2.682PheSer: 2.682 ± 0.517
3.203PheThr: 3.203 ± 0.539
3.203PheVal: 3.203 ± 0.477
0.372PheTrp: 0.372 ± 0.196
2.235PheTyr: 2.235 ± 0.45
0.0PheXaa: 0.0 ± 0.0
Gly
4.321GlyAla: 4.321 ± 0.697
0.372GlyCys: 0.372 ± 0.173
3.427GlyAsp: 3.427 ± 0.666
3.129GlyGlu: 3.129 ± 0.61
2.905GlyPhe: 2.905 ± 0.504
3.054GlyGly: 3.054 ± 0.655
1.639GlyHis: 1.639 ± 0.373
4.47GlyIle: 4.47 ± 0.567
5.959GlyLys: 5.959 ± 0.676
4.395GlyLeu: 4.395 ± 0.807
1.713GlyMet: 1.713 ± 0.272
3.501GlyAsn: 3.501 ± 0.518
0.298GlyPro: 0.298 ± 0.157
2.756GlyGln: 2.756 ± 0.363
2.16GlyArg: 2.16 ± 0.377
2.831GlySer: 2.831 ± 0.58
4.023GlyThr: 4.023 ± 0.573
4.842GlyVal: 4.842 ± 0.947
0.819GlyTrp: 0.819 ± 0.273
2.533GlyTyr: 2.533 ± 0.422
0.0GlyXaa: 0.0 ± 0.0
His
1.49HisAla: 1.49 ± 0.289
0.0HisCys: 0.0 ± 0.0
0.968HisAsp: 0.968 ± 0.32
1.043HisGlu: 1.043 ± 0.343
0.894HisPhe: 0.894 ± 0.269
1.043HisGly: 1.043 ± 0.269
0.447HisHis: 0.447 ± 0.186
1.49HisIle: 1.49 ± 0.325
1.192HisLys: 1.192 ± 0.254
1.49HisLeu: 1.49 ± 0.327
0.372HisMet: 0.372 ± 0.15
1.117HisAsn: 1.117 ± 0.242
1.043HisPro: 1.043 ± 0.275
0.894HisGln: 0.894 ± 0.254
0.521HisArg: 0.521 ± 0.188
1.043HisSer: 1.043 ± 0.294
1.415HisThr: 1.415 ± 0.323
0.67HisVal: 0.67 ± 0.229
0.074HisTrp: 0.074 ± 0.068
1.192HisTyr: 1.192 ± 0.427
0.0HisXaa: 0.0 ± 0.0
Ile
4.693IleAla: 4.693 ± 0.835
0.298IleCys: 0.298 ± 0.16
5.438IleAsp: 5.438 ± 0.632
5.959IleGlu: 5.959 ± 0.805
3.352IlePhe: 3.352 ± 0.505
5.215IleGly: 5.215 ± 0.867
0.894IleHis: 0.894 ± 0.249
3.874IleIle: 3.874 ± 0.471
8.343IleLys: 8.343 ± 0.798
3.352IleLeu: 3.352 ± 0.487
1.862IleMet: 1.862 ± 0.376
5.14IleAsn: 5.14 ± 0.784
2.756IlePro: 2.756 ± 0.42
3.203IleGln: 3.203 ± 0.461
3.65IleArg: 3.65 ± 0.542
4.991IleSer: 4.991 ± 0.681
5.215IleThr: 5.215 ± 0.697
4.023IleVal: 4.023 ± 0.524
1.043IleTrp: 1.043 ± 0.336
2.682IleTyr: 2.682 ± 0.563
0.0IleXaa: 0.0 ± 0.0
Lys
5.364LysAla: 5.364 ± 0.48
0.372LysCys: 0.372 ± 0.2
5.438LysAsp: 5.438 ± 0.616
8.343LysGlu: 8.343 ± 0.843
3.427LysPhe: 3.427 ± 0.576
5.587LysGly: 5.587 ± 0.666
2.16LysHis: 2.16 ± 0.391
6.555LysIle: 6.555 ± 0.761
7.524LysLys: 7.524 ± 0.839
5.81LysLeu: 5.81 ± 0.654
3.054LysMet: 3.054 ± 0.484
5.364LysAsn: 5.364 ± 0.624
3.054LysPro: 3.054 ± 0.505
3.65LysGln: 3.65 ± 0.579
4.395LysArg: 4.395 ± 0.54
4.768LysSer: 4.768 ± 0.607
5.736LysThr: 5.736 ± 0.65
5.736LysVal: 5.736 ± 0.706
0.819LysTrp: 0.819 ± 0.263
4.395LysTyr: 4.395 ± 0.67
0.0LysXaa: 0.0 ± 0.0
Leu
4.47LeuAla: 4.47 ± 0.622
0.223LeuCys: 0.223 ± 0.109
5.066LeuAsp: 5.066 ± 0.463
5.959LeuGlu: 5.959 ± 1.084
3.874LeuPhe: 3.874 ± 0.509
3.129LeuGly: 3.129 ± 0.515
1.266LeuHis: 1.266 ± 0.383
5.289LeuIle: 5.289 ± 0.549
5.513LeuLys: 5.513 ± 0.493
4.321LeuLeu: 4.321 ± 0.638
1.862LeuMet: 1.862 ± 0.478
6.108LeuAsn: 6.108 ± 0.663
2.16LeuPro: 2.16 ± 0.378
2.831LeuGln: 2.831 ± 0.402
3.203LeuArg: 3.203 ± 0.642
4.544LeuSer: 4.544 ± 0.529
5.215LeuThr: 5.215 ± 0.624
3.725LeuVal: 3.725 ± 0.508
0.447LeuTrp: 0.447 ± 0.244
3.725LeuTyr: 3.725 ± 0.582
0.0LeuXaa: 0.0 ± 0.0
Met
1.564MetAla: 1.564 ± 0.538
0.074MetCys: 0.074 ± 0.068
1.341MetAsp: 1.341 ± 0.321
1.713MetGlu: 1.713 ± 0.444
1.341MetPhe: 1.341 ± 0.338
1.043MetGly: 1.043 ± 0.283
0.447MetHis: 0.447 ± 0.194
1.788MetIle: 1.788 ± 0.283
2.086MetLys: 2.086 ± 0.467
2.235MetLeu: 2.235 ± 0.288
1.117MetMet: 1.117 ± 0.346
2.16MetAsn: 2.16 ± 0.401
1.043MetPro: 1.043 ± 0.234
1.713MetGln: 1.713 ± 0.455
0.596MetArg: 0.596 ± 0.199
1.788MetSer: 1.788 ± 0.45
2.011MetThr: 2.011 ± 0.413
0.819MetVal: 0.819 ± 0.209
0.372MetTrp: 0.372 ± 0.154
1.043MetTyr: 1.043 ± 0.277
0.0MetXaa: 0.0 ± 0.0
Asn
4.246AsnAla: 4.246 ± 0.621
0.372AsnCys: 0.372 ± 0.152
4.544AsnAsp: 4.544 ± 0.571
4.172AsnGlu: 4.172 ± 0.526
3.054AsnPhe: 3.054 ± 0.557
4.917AsnGly: 4.917 ± 0.693
0.894AsnHis: 0.894 ± 0.229
4.47AsnIle: 4.47 ± 0.739
6.63AsnLys: 6.63 ± 0.883
3.576AsnLeu: 3.576 ± 0.56
1.564AsnMet: 1.564 ± 0.37
5.364AsnAsn: 5.364 ± 0.888
2.607AsnPro: 2.607 ± 0.477
2.756AsnGln: 2.756 ± 0.484
2.309AsnArg: 2.309 ± 0.428
4.321AsnSer: 4.321 ± 0.582
3.948AsnThr: 3.948 ± 0.512
3.576AsnVal: 3.576 ± 0.601
0.745AsnTrp: 0.745 ± 0.255
2.682AsnTyr: 2.682 ± 0.428
0.0AsnXaa: 0.0 ± 0.0
Pro
1.192ProAla: 1.192 ± 0.262
0.0ProCys: 0.0 ± 0.0
1.862ProAsp: 1.862 ± 0.339
1.564ProGlu: 1.564 ± 0.335
1.49ProPhe: 1.49 ± 0.365
1.639ProGly: 1.639 ± 0.426
0.521ProHis: 0.521 ± 0.206
2.458ProIle: 2.458 ± 0.452
2.905ProLys: 2.905 ± 0.587
1.341ProLeu: 1.341 ± 0.279
0.819ProMet: 0.819 ± 0.273
1.639ProAsn: 1.639 ± 0.392
0.67ProPro: 0.67 ± 0.242
0.819ProGln: 0.819 ± 0.233
0.968ProArg: 0.968 ± 0.236
2.607ProSer: 2.607 ± 0.407
1.937ProThr: 1.937 ± 0.356
1.937ProVal: 1.937 ± 0.299
0.074ProTrp: 0.074 ± 0.079
1.266ProTyr: 1.266 ± 0.327
0.0ProXaa: 0.0 ± 0.0
Gln
3.203GlnAla: 3.203 ± 0.464
0.447GlnCys: 0.447 ± 0.169
2.086GlnAsp: 2.086 ± 0.442
2.756GlnGlu: 2.756 ± 0.534
1.862GlnPhe: 1.862 ± 0.373
2.533GlnGly: 2.533 ± 0.425
0.819GlnHis: 0.819 ± 0.19
3.129GlnIle: 3.129 ± 0.47
2.607GlnLys: 2.607 ± 0.514
2.533GlnLeu: 2.533 ± 0.526
1.415GlnMet: 1.415 ± 0.35
2.905GlnAsn: 2.905 ± 0.383
1.117GlnPro: 1.117 ± 0.294
1.713GlnGln: 1.713 ± 0.442
2.309GlnArg: 2.309 ± 0.379
2.458GlnSer: 2.458 ± 0.406
1.639GlnThr: 1.639 ± 0.352
2.682GlnVal: 2.682 ± 0.546
0.223GlnTrp: 0.223 ± 0.107
1.788GlnTyr: 1.788 ± 0.418
0.0GlnXaa: 0.0 ± 0.0
Arg
1.564ArgAla: 1.564 ± 0.338
0.372ArgCys: 0.372 ± 0.167
2.756ArgAsp: 2.756 ± 0.45
3.576ArgGlu: 3.576 ± 0.517
1.713ArgPhe: 1.713 ± 0.369
1.937ArgGly: 1.937 ± 0.406
1.117ArgHis: 1.117 ± 0.336
3.65ArgIle: 3.65 ± 0.489
3.576ArgLys: 3.576 ± 0.516
3.576ArgLeu: 3.576 ± 0.624
0.968ArgMet: 0.968 ± 0.244
2.607ArgAsn: 2.607 ± 0.437
1.117ArgPro: 1.117 ± 0.262
2.086ArgGln: 2.086 ± 0.41
1.341ArgArg: 1.341 ± 0.27
1.564ArgSer: 1.564 ± 0.311
1.862ArgThr: 1.862 ± 0.412
2.309ArgVal: 2.309 ± 0.364
0.372ArgTrp: 0.372 ± 0.155
2.458ArgTyr: 2.458 ± 0.445
0.0ArgXaa: 0.0 ± 0.0
Ser
3.948SerAla: 3.948 ± 0.541
0.074SerCys: 0.074 ± 0.074
4.544SerAsp: 4.544 ± 0.584
3.576SerGlu: 3.576 ± 0.575
2.607SerPhe: 2.607 ± 0.449
4.172SerGly: 4.172 ± 0.659
1.266SerHis: 1.266 ± 0.252
5.438SerIle: 5.438 ± 0.637
5.662SerLys: 5.662 ± 0.694
4.321SerLeu: 4.321 ± 0.546
1.713SerMet: 1.713 ± 0.32
4.619SerAsn: 4.619 ± 0.649
0.819SerPro: 0.819 ± 0.249
2.235SerGln: 2.235 ± 0.524
1.937SerArg: 1.937 ± 0.292
3.501SerSer: 3.501 ± 0.551
4.172SerThr: 4.172 ± 0.472
3.352SerVal: 3.352 ± 0.716
0.67SerTrp: 0.67 ± 0.208
1.937SerTyr: 1.937 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
4.246ThrAla: 4.246 ± 0.725
0.074ThrCys: 0.074 ± 0.076
3.799ThrAsp: 3.799 ± 0.467
3.948ThrGlu: 3.948 ± 0.399
2.16ThrPhe: 2.16 ± 0.526
4.023ThrGly: 4.023 ± 0.556
1.341ThrHis: 1.341 ± 0.258
4.991ThrIle: 4.991 ± 0.731
4.917ThrLys: 4.917 ± 0.607
4.693ThrLeu: 4.693 ± 0.493
1.415ThrMet: 1.415 ± 0.381
4.47ThrAsn: 4.47 ± 0.697
1.788ThrPro: 1.788 ± 0.339
2.309ThrGln: 2.309 ± 0.442
2.309ThrArg: 2.309 ± 0.431
4.172ThrSer: 4.172 ± 0.768
3.799ThrThr: 3.799 ± 0.527
3.725ThrVal: 3.725 ± 0.614
0.968ThrTrp: 0.968 ± 0.289
2.16ThrTyr: 2.16 ± 0.36
0.0ThrXaa: 0.0 ± 0.0
Val
5.066ValAla: 5.066 ± 0.794
0.223ValCys: 0.223 ± 0.116
4.693ValAsp: 4.693 ± 0.674
4.693ValGlu: 4.693 ± 0.745
2.086ValPhe: 2.086 ± 0.339
3.65ValGly: 3.65 ± 0.697
0.447ValHis: 0.447 ± 0.175
4.768ValIle: 4.768 ± 0.526
6.481ValLys: 6.481 ± 0.64
4.917ValLeu: 4.917 ± 0.56
1.639ValMet: 1.639 ± 0.376
3.725ValAsn: 3.725 ± 0.646
2.011ValPro: 2.011 ± 0.395
1.564ValGln: 1.564 ± 0.475
2.16ValArg: 2.16 ± 0.371
3.874ValSer: 3.874 ± 0.642
3.352ValThr: 3.352 ± 0.7
3.576ValVal: 3.576 ± 0.526
0.596ValTrp: 0.596 ± 0.237
2.086ValTyr: 2.086 ± 0.461
0.0ValXaa: 0.0 ± 0.0
Trp
0.596TrpAla: 0.596 ± 0.199
0.074TrpCys: 0.074 ± 0.061
0.447TrpAsp: 0.447 ± 0.183
0.968TrpGlu: 0.968 ± 0.28
0.67TrpPhe: 0.67 ± 0.21
0.819TrpGly: 0.819 ± 0.24
0.298TrpHis: 0.298 ± 0.136
0.745TrpIle: 0.745 ± 0.234
0.894TrpLys: 0.894 ± 0.256
1.192TrpLeu: 1.192 ± 0.319
0.223TrpMet: 0.223 ± 0.133
1.117TrpAsn: 1.117 ± 0.35
0.0TrpPro: 0.0 ± 0.0
0.596TrpGln: 0.596 ± 0.235
0.298TrpArg: 0.298 ± 0.166
0.745TrpSer: 0.745 ± 0.275
0.894TrpThr: 0.894 ± 0.214
0.745TrpVal: 0.745 ± 0.248
0.149TrpTrp: 0.149 ± 0.11
0.372TrpTyr: 0.372 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.639TyrAla: 1.639 ± 0.364
0.223TyrCys: 0.223 ± 0.129
1.713TyrAsp: 1.713 ± 0.413
3.65TyrGlu: 3.65 ± 0.534
1.639TyrPhe: 1.639 ± 0.492
2.086TyrGly: 2.086 ± 0.493
0.745TyrHis: 0.745 ± 0.245
3.129TyrIle: 3.129 ± 0.492
4.395TyrLys: 4.395 ± 0.639
3.501TyrLeu: 3.501 ± 0.591
0.596TyrMet: 0.596 ± 0.217
3.129TyrAsn: 3.129 ± 0.528
1.415TyrPro: 1.415 ± 0.317
1.639TyrGln: 1.639 ± 0.288
2.458TyrArg: 2.458 ± 0.544
2.607TyrSer: 2.607 ± 0.487
2.533TyrThr: 2.533 ± 0.44
3.203TyrVal: 3.203 ± 0.607
0.968TyrTrp: 0.968 ± 0.401
1.49TyrTyr: 1.49 ± 0.391
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (13425 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski