Amino acid dipepetide frequency for Streptomyces phage BillNye

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.186AlaAla: 9.186 ± 1.146
0.866AlaCys: 0.866 ± 0.208
4.104AlaAsp: 4.104 ± 0.298
6.31AlaGlu: 6.31 ± 0.642
3.462AlaPhe: 3.462 ± 0.288
7.036AlaGly: 7.036 ± 0.721
1.368AlaHis: 1.368 ± 0.16
4.412AlaIle: 4.412 ± 0.411
5.054AlaLys: 5.054 ± 0.399
6.282AlaLeu: 6.282 ± 0.535
2.792AlaMet: 2.792 ± 0.377
3.825AlaAsn: 3.825 ± 0.402
3.881AlaPro: 3.881 ± 0.495
3.378AlaGln: 3.378 ± 0.486
4.802AlaArg: 4.802 ± 0.453
4.914AlaSer: 4.914 ± 0.513
5.752AlaThr: 5.752 ± 0.58
5.752AlaVal: 5.752 ± 0.387
1.871AlaTrp: 1.871 ± 0.238
3.155AlaTyr: 3.155 ± 0.268
0.0AlaXaa: 0.0 ± 0.0
Cys
0.726CysAla: 0.726 ± 0.155
0.251CysCys: 0.251 ± 0.091
0.698CysAsp: 0.698 ± 0.124
1.201CysGlu: 1.201 ± 0.231
0.503CysPhe: 0.503 ± 0.135
1.424CysGly: 1.424 ± 0.245
0.195CysHis: 0.195 ± 0.07
0.475CysIle: 0.475 ± 0.128
0.475CysLys: 0.475 ± 0.122
0.921CysLeu: 0.921 ± 0.195
0.307CysMet: 0.307 ± 0.089
0.419CysAsn: 0.419 ± 0.117
0.754CysPro: 0.754 ± 0.189
0.447CysGln: 0.447 ± 0.117
0.642CysArg: 0.642 ± 0.14
0.838CysSer: 0.838 ± 0.164
0.307CysThr: 0.307 ± 0.091
0.67CysVal: 0.67 ± 0.166
0.168CysTrp: 0.168 ± 0.068
0.475CysTyr: 0.475 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
5.221AspAla: 5.221 ± 0.407
1.061AspCys: 1.061 ± 0.199
3.267AspAsp: 3.267 ± 0.343
5.445AspGlu: 5.445 ± 0.421
2.764AspPhe: 2.764 ± 0.327
5.5AspGly: 5.5 ± 0.444
0.977AspHis: 0.977 ± 0.182
3.183AspIle: 3.183 ± 0.345
3.211AspLys: 3.211 ± 0.311
4.467AspLeu: 4.467 ± 0.36
1.871AspMet: 1.871 ± 0.242
2.625AspAsn: 2.625 ± 0.279
2.401AspPro: 2.401 ± 0.276
1.647AspGln: 1.647 ± 0.2
2.569AspArg: 2.569 ± 0.283
3.965AspSer: 3.965 ± 0.426
3.714AspThr: 3.714 ± 0.364
4.467AspVal: 4.467 ± 0.383
1.508AspTrp: 1.508 ± 0.217
2.876AspTyr: 2.876 ± 0.248
0.0AspXaa: 0.0 ± 0.0
Glu
5.919GluAla: 5.919 ± 0.504
0.726GluCys: 0.726 ± 0.186
4.132GluAsp: 4.132 ± 0.41
4.551GluGlu: 4.551 ± 0.506
3.49GluPhe: 3.49 ± 0.362
4.3GluGly: 4.3 ± 0.378
1.48GluHis: 1.48 ± 0.257
4.077GluIle: 4.077 ± 0.355
4.802GluLys: 4.802 ± 0.366
5.863GluLeu: 5.863 ± 0.445
1.954GluMet: 1.954 ± 0.26
2.988GluAsn: 2.988 ± 0.307
1.871GluPro: 1.871 ± 0.23
2.234GluGln: 2.234 ± 0.32
3.853GluArg: 3.853 ± 0.315
2.513GluSer: 2.513 ± 0.318
3.434GluThr: 3.434 ± 0.37
4.914GluVal: 4.914 ± 0.43
1.564GluTrp: 1.564 ± 0.197
2.988GluTyr: 2.988 ± 0.303
0.0GluXaa: 0.0 ± 0.0
Phe
2.541PheAla: 2.541 ± 0.287
0.586PheCys: 0.586 ± 0.137
3.043PheAsp: 3.043 ± 0.267
3.071PheGlu: 3.071 ± 0.282
1.564PhePhe: 1.564 ± 0.235
3.183PheGly: 3.183 ± 0.277
0.531PheHis: 0.531 ± 0.139
1.759PheIle: 1.759 ± 0.248
2.094PheLys: 2.094 ± 0.269
2.876PheLeu: 2.876 ± 0.313
1.061PheMet: 1.061 ± 0.187
1.982PheAsn: 1.982 ± 0.254
1.368PhePro: 1.368 ± 0.182
1.145PheGln: 1.145 ± 0.211
2.094PheArg: 2.094 ± 0.295
2.485PheSer: 2.485 ± 0.287
2.82PheThr: 2.82 ± 0.25
2.68PheVal: 2.68 ± 0.28
0.698PheTrp: 0.698 ± 0.154
1.229PheTyr: 1.229 ± 0.159
0.0PheXaa: 0.0 ± 0.0
Gly
5.138GlyAla: 5.138 ± 0.415
0.921GlyCys: 0.921 ± 0.165
5.082GlyAsp: 5.082 ± 0.442
3.965GlyGlu: 3.965 ± 0.386
3.211GlyPhe: 3.211 ± 0.34
7.148GlyGly: 7.148 ± 0.782
1.759GlyHis: 1.759 ± 0.206
4.467GlyIle: 4.467 ± 0.341
5.054GlyLys: 5.054 ± 0.47
6.003GlyLeu: 6.003 ± 0.483
2.29GlyMet: 2.29 ± 0.27
3.825GlyAsn: 3.825 ± 0.375
2.429GlyPro: 2.429 ± 0.279
2.206GlyGln: 2.206 ± 0.25
3.993GlyArg: 3.993 ± 0.346
4.495GlySer: 4.495 ± 0.491
5.528GlyThr: 5.528 ± 0.75
6.199GlyVal: 6.199 ± 0.433
2.038GlyTrp: 2.038 ± 0.221
3.741GlyTyr: 3.741 ± 0.411
0.0GlyXaa: 0.0 ± 0.0
His
1.48HisAla: 1.48 ± 0.217
0.195HisCys: 0.195 ± 0.065
1.34HisAsp: 1.34 ± 0.219
0.977HisGlu: 0.977 ± 0.18
0.614HisPhe: 0.614 ± 0.154
1.619HisGly: 1.619 ± 0.24
0.475HisHis: 0.475 ± 0.111
0.866HisIle: 0.866 ± 0.157
1.117HisLys: 1.117 ± 0.136
1.089HisLeu: 1.089 ± 0.173
0.531HisMet: 0.531 ± 0.123
0.754HisAsn: 0.754 ± 0.119
0.726HisPro: 0.726 ± 0.152
0.782HisGln: 0.782 ± 0.168
1.033HisArg: 1.033 ± 0.174
0.866HisSer: 0.866 ± 0.144
0.754HisThr: 0.754 ± 0.144
1.117HisVal: 1.117 ± 0.184
0.279HisTrp: 0.279 ± 0.09
0.893HisTyr: 0.893 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
4.328IleAla: 4.328 ± 0.433
0.838IleCys: 0.838 ± 0.189
4.16IleAsp: 4.16 ± 0.352
3.769IleGlu: 3.769 ± 0.355
1.368IlePhe: 1.368 ± 0.185
4.188IleGly: 4.188 ± 0.301
0.866IleHis: 0.866 ± 0.156
2.597IleIle: 2.597 ± 0.291
2.764IleLys: 2.764 ± 0.252
3.546IleLeu: 3.546 ± 0.315
1.256IleMet: 1.256 ± 0.192
1.843IleAsn: 1.843 ± 0.215
2.29IlePro: 2.29 ± 0.278
1.647IleGln: 1.647 ± 0.202
3.211IleArg: 3.211 ± 0.261
3.099IleSer: 3.099 ± 0.297
3.127IleThr: 3.127 ± 0.342
3.49IleVal: 3.49 ± 0.358
0.81IleTrp: 0.81 ± 0.172
1.703IleTyr: 1.703 ± 0.286
0.0IleXaa: 0.0 ± 0.0
Lys
6.254LysAla: 6.254 ± 0.603
0.503LysCys: 0.503 ± 0.141
2.876LysAsp: 2.876 ± 0.311
2.848LysGlu: 2.848 ± 0.405
2.429LysPhe: 2.429 ± 0.291
4.049LysGly: 4.049 ± 0.353
1.368LysHis: 1.368 ± 0.241
2.708LysIle: 2.708 ± 0.29
4.384LysLys: 4.384 ± 0.443
3.546LysLeu: 3.546 ± 0.275
2.01LysMet: 2.01 ± 0.232
2.764LysAsn: 2.764 ± 0.315
3.043LysPro: 3.043 ± 0.375
2.15LysGln: 2.15 ± 0.308
4.495LysArg: 4.495 ± 0.386
3.462LysSer: 3.462 ± 0.348
3.239LysThr: 3.239 ± 0.3
4.579LysVal: 4.579 ± 0.44
1.089LysTrp: 1.089 ± 0.197
2.429LysTyr: 2.429 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
7.232LeuAla: 7.232 ± 0.465
0.726LeuCys: 0.726 ± 0.174
5.249LeuAsp: 5.249 ± 0.407
5.193LeuGlu: 5.193 ± 0.383
2.597LeuPhe: 2.597 ± 0.28
5.417LeuGly: 5.417 ± 0.416
1.34LeuHis: 1.34 ± 0.173
3.658LeuIle: 3.658 ± 0.332
4.495LeuLys: 4.495 ± 0.349
4.272LeuLeu: 4.272 ± 0.441
2.373LeuMet: 2.373 ± 0.249
3.099LeuAsn: 3.099 ± 0.343
2.513LeuPro: 2.513 ± 0.27
2.066LeuGln: 2.066 ± 0.249
3.63LeuArg: 3.63 ± 0.312
4.356LeuSer: 4.356 ± 0.387
4.858LeuThr: 4.858 ± 0.421
4.747LeuVal: 4.747 ± 0.373
1.312LeuTrp: 1.312 ± 0.205
2.708LeuTyr: 2.708 ± 0.303
0.0LeuXaa: 0.0 ± 0.0
Met
3.351MetAla: 3.351 ± 0.366
0.279MetCys: 0.279 ± 0.085
1.396MetAsp: 1.396 ± 0.207
1.452MetGlu: 1.452 ± 0.22
1.145MetPhe: 1.145 ± 0.213
1.619MetGly: 1.619 ± 0.224
0.391MetHis: 0.391 ± 0.097
1.145MetIle: 1.145 ± 0.185
1.759MetLys: 1.759 ± 0.245
2.094MetLeu: 2.094 ± 0.296
0.81MetMet: 0.81 ± 0.182
1.061MetAsn: 1.061 ± 0.164
1.117MetPro: 1.117 ± 0.147
0.977MetGln: 0.977 ± 0.183
1.424MetArg: 1.424 ± 0.217
2.262MetSer: 2.262 ± 0.266
1.982MetThr: 1.982 ± 0.2
1.899MetVal: 1.899 ± 0.269
0.447MetTrp: 0.447 ± 0.1
0.726MetTyr: 0.726 ± 0.136
0.0MetXaa: 0.0 ± 0.0
Asn
4.132AsnAla: 4.132 ± 0.372
0.363AsnCys: 0.363 ± 0.111
3.071AsnAsp: 3.071 ± 0.239
2.82AsnGlu: 2.82 ± 0.303
1.564AsnPhe: 1.564 ± 0.218
4.216AsnGly: 4.216 ± 0.339
0.81AsnHis: 0.81 ± 0.163
2.178AsnIle: 2.178 ± 0.23
2.457AsnLys: 2.457 ± 0.26
3.071AsnLeu: 3.071 ± 0.281
0.893AsnMet: 0.893 ± 0.145
1.564AsnAsn: 1.564 ± 0.214
2.429AsnPro: 2.429 ± 0.242
1.033AsnGln: 1.033 ± 0.188
2.094AsnArg: 2.094 ± 0.238
2.541AsnSer: 2.541 ± 0.278
2.206AsnThr: 2.206 ± 0.33
3.015AsnVal: 3.015 ± 0.276
0.726AsnTrp: 0.726 ± 0.11
1.759AsnTyr: 1.759 ± 0.193
0.0AsnXaa: 0.0 ± 0.0
Pro
3.462ProAla: 3.462 ± 0.533
0.475ProCys: 0.475 ± 0.123
2.597ProAsp: 2.597 ± 0.294
2.876ProGlu: 2.876 ± 0.302
1.424ProPhe: 1.424 ± 0.188
3.49ProGly: 3.49 ± 0.326
0.531ProHis: 0.531 ± 0.123
2.066ProIle: 2.066 ± 0.237
2.513ProLys: 2.513 ± 0.324
2.513ProLeu: 2.513 ± 0.229
0.67ProMet: 0.67 ± 0.144
1.899ProAsn: 1.899 ± 0.213
1.256ProPro: 1.256 ± 0.239
1.201ProGln: 1.201 ± 0.189
2.038ProArg: 2.038 ± 0.26
2.373ProSer: 2.373 ± 0.272
2.736ProThr: 2.736 ± 0.397
3.267ProVal: 3.267 ± 0.318
0.363ProTrp: 0.363 ± 0.102
1.396ProTyr: 1.396 ± 0.208
0.0ProXaa: 0.0 ± 0.0
Gln
3.574GlnAla: 3.574 ± 0.398
0.307GlnCys: 0.307 ± 0.077
1.703GlnAsp: 1.703 ± 0.178
1.982GlnGlu: 1.982 ± 0.311
1.173GlnPhe: 1.173 ± 0.137
2.094GlnGly: 2.094 ± 0.23
0.419GlnHis: 0.419 ± 0.108
1.703GlnIle: 1.703 ± 0.181
2.15GlnLys: 2.15 ± 0.342
2.876GlnLeu: 2.876 ± 0.254
0.698GlnMet: 0.698 ± 0.173
1.117GlnAsn: 1.117 ± 0.155
0.977GlnPro: 0.977 ± 0.192
1.284GlnGln: 1.284 ± 0.237
1.927GlnArg: 1.927 ± 0.271
1.731GlnSer: 1.731 ± 0.197
1.256GlnThr: 1.256 ± 0.201
2.401GlnVal: 2.401 ± 0.262
0.698GlnTrp: 0.698 ± 0.137
1.005GlnTyr: 1.005 ± 0.133
0.0GlnXaa: 0.0 ± 0.0
Arg
4.858ArgAla: 4.858 ± 0.366
0.531ArgCys: 0.531 ± 0.137
2.792ArgAsp: 2.792 ± 0.31
4.439ArgGlu: 4.439 ± 0.422
1.899ArgPhe: 1.899 ± 0.21
3.769ArgGly: 3.769 ± 0.354
0.893ArgHis: 0.893 ± 0.161
3.546ArgIle: 3.546 ± 0.222
3.63ArgLys: 3.63 ± 0.401
3.853ArgLeu: 3.853 ± 0.316
1.675ArgMet: 1.675 ± 0.241
2.373ArgAsn: 2.373 ± 0.274
2.066ArgPro: 2.066 ± 0.202
1.424ArgGln: 1.424 ± 0.243
3.211ArgArg: 3.211 ± 0.431
2.876ArgSer: 2.876 ± 0.264
2.792ArgThr: 2.792 ± 0.266
4.216ArgVal: 4.216 ± 0.357
0.977ArgTrp: 0.977 ± 0.181
2.792ArgTyr: 2.792 ± 0.249
0.0ArgXaa: 0.0 ± 0.0
Ser
4.97SerAla: 4.97 ± 0.484
0.558SerCys: 0.558 ± 0.118
4.077SerAsp: 4.077 ± 0.404
3.686SerGlu: 3.686 ± 0.344
2.513SerPhe: 2.513 ± 0.325
5.64SerGly: 5.64 ± 0.695
1.117SerHis: 1.117 ± 0.171
2.345SerIle: 2.345 ± 0.262
3.518SerLys: 3.518 ± 0.372
4.747SerLeu: 4.747 ± 0.367
1.508SerMet: 1.508 ± 0.278
2.29SerAsn: 2.29 ± 0.259
2.345SerPro: 2.345 ± 0.248
1.899SerGln: 1.899 ± 0.207
2.932SerArg: 2.932 ± 0.334
4.802SerSer: 4.802 ± 0.662
3.63SerThr: 3.63 ± 0.443
3.881SerVal: 3.881 ± 0.44
1.089SerTrp: 1.089 ± 0.196
2.122SerTyr: 2.122 ± 0.209
0.0SerXaa: 0.0 ± 0.0
Thr
5.752ThrAla: 5.752 ± 0.65
0.642ThrCys: 0.642 ± 0.134
3.797ThrAsp: 3.797 ± 0.376
3.993ThrGlu: 3.993 ± 0.411
2.569ThrPhe: 2.569 ± 0.267
5.668ThrGly: 5.668 ± 0.651
0.81ThrHis: 0.81 ± 0.166
3.099ThrIle: 3.099 ± 0.326
2.708ThrLys: 2.708 ± 0.284
4.719ThrLeu: 4.719 ± 0.436
1.312ThrMet: 1.312 ± 0.173
2.513ThrAsn: 2.513 ± 0.264
3.267ThrPro: 3.267 ± 0.434
1.899ThrGln: 1.899 ± 0.269
2.848ThrArg: 2.848 ± 0.28
3.155ThrSer: 3.155 ± 0.336
4.719ThrThr: 4.719 ± 0.792
5.556ThrVal: 5.556 ± 0.493
1.284ThrTrp: 1.284 ± 0.209
2.206ThrTyr: 2.206 ± 0.332
0.0ThrXaa: 0.0 ± 0.0
Val
5.417ValAla: 5.417 ± 0.41
1.089ValCys: 1.089 ± 0.167
5.138ValAsp: 5.138 ± 0.376
5.5ValGlu: 5.5 ± 0.43
2.429ValPhe: 2.429 ± 0.226
4.188ValGly: 4.188 ± 0.397
1.033ValHis: 1.033 ± 0.186
4.16ValIle: 4.16 ± 0.335
5.054ValLys: 5.054 ± 0.469
4.858ValLeu: 4.858 ± 0.457
1.675ValMet: 1.675 ± 0.207
3.155ValAsn: 3.155 ± 0.349
2.653ValPro: 2.653 ± 0.333
2.038ValGln: 2.038 ± 0.257
4.188ValArg: 4.188 ± 0.338
4.775ValSer: 4.775 ± 0.392
5.026ValThr: 5.026 ± 0.45
6.45ValVal: 6.45 ± 0.498
1.368ValTrp: 1.368 ± 0.192
3.574ValTyr: 3.574 ± 0.314
0.0ValXaa: 0.0 ± 0.0
Trp
1.396TrpAla: 1.396 ± 0.191
0.363TrpCys: 0.363 ± 0.115
1.368TrpAsp: 1.368 ± 0.228
1.396TrpGlu: 1.396 ± 0.244
0.81TrpPhe: 0.81 ± 0.188
1.452TrpGly: 1.452 ± 0.19
0.503TrpHis: 0.503 ± 0.116
0.698TrpIle: 0.698 ± 0.153
1.033TrpLys: 1.033 ± 0.168
1.312TrpLeu: 1.312 ± 0.235
0.586TrpMet: 0.586 ± 0.143
1.033TrpAsn: 1.033 ± 0.156
0.475TrpPro: 0.475 ± 0.098
0.586TrpGln: 0.586 ± 0.132
1.173TrpArg: 1.173 ± 0.174
1.284TrpSer: 1.284 ± 0.17
1.619TrpThr: 1.619 ± 0.308
1.284TrpVal: 1.284 ± 0.187
0.307TrpTrp: 0.307 ± 0.088
0.698TrpTyr: 0.698 ± 0.147
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.267TyrAla: 3.267 ± 0.298
0.614TyrCys: 0.614 ± 0.139
2.848TyrAsp: 2.848 ± 0.268
2.178TyrGlu: 2.178 ± 0.281
1.284TyrPhe: 1.284 ± 0.202
3.49TyrGly: 3.49 ± 0.271
0.642TyrHis: 0.642 ± 0.127
1.759TyrIle: 1.759 ± 0.215
1.954TyrLys: 1.954 ± 0.237
2.932TyrLeu: 2.932 ± 0.305
1.033TyrMet: 1.033 ± 0.175
1.815TyrAsn: 1.815 ± 0.224
1.284TyrPro: 1.284 ± 0.237
1.033TyrGln: 1.033 ± 0.153
2.457TyrArg: 2.457 ± 0.276
2.904TyrSer: 2.904 ± 0.315
3.015TyrThr: 3.015 ± 0.315
3.183TyrVal: 3.183 ± 0.288
0.782TyrTrp: 0.782 ± 0.153
1.815TyrTyr: 1.815 ± 0.291
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 212 proteins (35816 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski