Sweet potato symptomless virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 8.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2GUW7|A0A2Z2GUW7_9GEMI Isoform of A0A2Z2GPB7 Replication-associated protein OS=Sweet potato symptomless virus 1 OX=603333 GN=C1 PE=3 SV=1
MM1 pKa = 7.57PRR3 pKa = 11.84QPRR6 pKa = 11.84ARR8 pKa = 11.84SVFSFSSKK16 pKa = 10.93YY17 pKa = 10.87GFLTYY22 pKa = 9.81SQCDD26 pKa = 3.51LTAEE30 pKa = 5.44IIQQMLITLLTPFNLLFLAVAPEE53 pKa = 3.8HH54 pKa = 6.4HH55 pKa = 6.77QDD57 pKa = 3.29GSLHH61 pKa = 5.04FHH63 pKa = 6.59VLFQCARR70 pKa = 11.84RR71 pKa = 11.84IITRR75 pKa = 11.84RR76 pKa = 11.84VDD78 pKa = 5.13FFDD81 pKa = 4.53LNNYY85 pKa = 9.02HH86 pKa = 7.26PNIQPARR93 pKa = 11.84DD94 pKa = 3.35SAAVLEE100 pKa = 4.86YY101 pKa = 10.06ISKK104 pKa = 8.8EE105 pKa = 3.66QPPIVWGEE113 pKa = 3.86FQNHH117 pKa = 5.26KK118 pKa = 10.0VSPSRR123 pKa = 11.84RR124 pKa = 11.84DD125 pKa = 3.41DD126 pKa = 2.92RR127 pKa = 11.84WRR129 pKa = 11.84DD130 pKa = 3.42IIFTSTSKK138 pKa = 11.14AEE140 pKa = 3.78YY141 pKa = 10.06LNRR144 pKa = 11.84VKK146 pKa = 10.73QEE148 pKa = 3.95FPADD152 pKa = 3.41YY153 pKa = 8.2ATRR156 pKa = 11.84LQQLEE161 pKa = 4.41YY162 pKa = 9.88FAEE165 pKa = 4.14RR166 pKa = 11.84LFPSTAPPYY175 pKa = 10.46VSPFDD180 pKa = 4.21PLTLQCHH187 pKa = 6.24EE188 pKa = 6.13DD189 pKa = 3.21IQDD192 pKa = 3.9WINRR196 pKa = 11.84EE197 pKa = 3.9LYY199 pKa = 10.19LVHH202 pKa = 7.99LEE204 pKa = 4.53DD205 pKa = 5.4YY206 pKa = 10.82ILIHH210 pKa = 6.11NCSYY214 pKa = 10.1DD215 pKa = 3.32TARR218 pKa = 11.84QDD220 pKa = 5.57LEE222 pKa = 4.21WLDD225 pKa = 4.08DD226 pKa = 3.94LARR229 pKa = 11.84NPVKK233 pKa = 10.44EE234 pKa = 4.13PSEE237 pKa = 4.42DD238 pKa = 3.81PGPSTYY244 pKa = 10.39VVPPAQQRR252 pKa = 11.84QPGQDD257 pKa = 3.06NSGGTTTGTVGLTSPRR273 pKa = 11.84TTVMPHH279 pKa = 4.65TTSPTTSHH287 pKa = 6.57SNSSQAGSNN296 pKa = 3.38

Molecular weight: 33.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8ZUC4|A0A2U8ZUC4_9GEMI Capsid protein OS=Sweet potato symptomless virus 1 OX=603333 GN=V2 PE=3 SV=1
MM1 pKa = 7.13VFSPTLNVISQLRR14 pKa = 11.84SFSKK18 pKa = 10.63CLSLSSHH25 pKa = 6.46LLIFSFSLLLLNIIRR40 pKa = 11.84MVPSIFTFSFNVLVGLSLVGSISLILIITIQTYY73 pKa = 10.64NLLVTLQQSLNTYY86 pKa = 8.02QRR88 pKa = 11.84NNLLSSGGNSRR99 pKa = 11.84IIKK102 pKa = 9.36YY103 pKa = 9.77RR104 pKa = 11.84LHH106 pKa = 6.92EE107 pKa = 4.45GMTDD111 pKa = 3.71GEE113 pKa = 4.23ISSLRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84LRR122 pKa = 11.84RR123 pKa = 11.84STLTEE128 pKa = 3.67

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

2

5

1097

86

335

219.4

25.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.105 ± 0.882

1.459 ± 0.286

5.014 ± 0.837

3.464 ± 0.769

5.561 ± 0.346

5.105 ± 1.051

2.37 ± 0.585

6.016 ± 0.672

4.284 ± 1.094

9.116 ± 1.657

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.641 ± 0.346

4.284 ± 0.3

6.381 ± 1.05

5.469 ± 0.475

6.837 ± 0.352

8.295 ± 1.252

7.475 ± 0.582

6.29 ± 1.213

1.641 ± 0.407

4.193 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski