Methylobacterium durans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8WBA8|A0A2U8WBA8_9RHIZ Uncharacterized protein OS=Methylobacterium durans OX=2202825 GN=DK389_26710 PE=4 SV=1
MM1 pKa = 7.63FDD3 pKa = 3.31VDD5 pKa = 3.62QNEE8 pKa = 4.02AAFTFDD14 pKa = 5.17LDD16 pKa = 4.17EE17 pKa = 4.69EE18 pKa = 5.01DD19 pKa = 4.11EE20 pKa = 4.32LVTYY24 pKa = 10.33GVGPCIAIAVVNQTQAWAGLCHH46 pKa = 6.92YY47 pKa = 10.69DD48 pKa = 3.74NVHH51 pKa = 6.27HH52 pKa = 6.49RR53 pKa = 11.84TPDD56 pKa = 3.23LRR58 pKa = 11.84GFLSAARR65 pKa = 11.84QACEE69 pKa = 3.82PGDD72 pKa = 3.72AVEE75 pKa = 4.1VWFGGGDD82 pKa = 3.43NTDD85 pKa = 3.9PEE87 pKa = 4.53TADD90 pKa = 3.25EE91 pKa = 4.29VNRR94 pKa = 11.84ARR96 pKa = 11.84EE97 pKa = 3.94AAQAAVQAYY106 pKa = 8.81FPLTQQPLWLDD117 pKa = 3.07AGGAYY122 pKa = 8.7TVIVSATSADD132 pKa = 3.31IAFEE136 pKa = 4.07LAPDD140 pKa = 3.88DD141 pKa = 4.0WSTGQQ146 pKa = 3.58

Molecular weight: 15.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8WCI0|A0A2U8WCI0_9RHIZ Uncharacterized protein OS=Methylobacterium durans OX=2202825 GN=DK389_25910 PE=4 SV=1
MM1 pKa = 7.54NATQPALGAPSWPISTRR18 pKa = 11.84SIPATTSPPRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84NPRR33 pKa = 11.84RR34 pKa = 11.84PTPRR38 pKa = 11.84RR39 pKa = 11.84LPPRR43 pKa = 11.84RR44 pKa = 11.84IRR46 pKa = 11.84PSRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84TSTPASPSRR60 pKa = 3.56

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5545

0

5545

1576948

29

4713

284.4

30.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.958 ± 0.053

0.863 ± 0.011

5.497 ± 0.028

5.665 ± 0.033

3.356 ± 0.019

9.001 ± 0.036

2.026 ± 0.016

4.414 ± 0.025

2.493 ± 0.03

10.395 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.016

2.196 ± 0.022

5.756 ± 0.033

2.879 ± 0.022

8.32 ± 0.05

5.154 ± 0.027

5.317 ± 0.036

7.399 ± 0.03

1.263 ± 0.013

2.044 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski