Pontibacter sp. GY10130

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Pontibacter; unclassified Pontibacter

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5C8KB90|A0A5C8KB90_9BACT Uncharacterized protein OS=Pontibacter sp. GY10130 OX=2603253 GN=FVR03_03740 PE=4 SV=1
MM1 pKa = 7.18TPHH4 pKa = 7.43IILLTQEE11 pKa = 4.44PNADD15 pKa = 2.81MRR17 pKa = 11.84FYY19 pKa = 9.3YY20 pKa = 10.13TADD23 pKa = 3.23IAHH26 pKa = 6.85TDD28 pKa = 3.36DD29 pKa = 5.77DD30 pKa = 4.46GALKK34 pKa = 10.41VFPSFDD40 pKa = 3.24AAADD44 pKa = 3.69EE45 pKa = 4.84RR46 pKa = 11.84DD47 pKa = 3.6DD48 pKa = 5.65LEE50 pKa = 5.6IDD52 pKa = 4.06GYY54 pKa = 10.65IIEE57 pKa = 4.62LPIYY61 pKa = 10.28SII63 pKa = 4.52

Molecular weight:
7.15 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5C8J567|A0A5C8J567_9BACT Uncharacterized protein OS=Pontibacter sp. GY10130 OX=2603253 GN=FVR03_20020 PE=4 SV=1
MM1 pKa = 7.52LLFRR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 9.42SALDD11 pKa = 3.56KK12 pKa = 10.95PPEE15 pKa = 4.45GSDD18 pKa = 3.73ARR20 pKa = 11.84DD21 pKa = 3.02KK22 pKa = 11.04AARR25 pKa = 11.84AIAAVIVNRR34 pKa = 11.84QLQLAKK40 pKa = 9.92CCSSRR45 pKa = 11.84YY46 pKa = 8.97EE47 pKa = 3.98RR48 pKa = 11.84LSLGVRR54 pKa = 11.84KK55 pKa = 9.65ALLLLLCMLFGGTSLWFLARR75 pKa = 11.84GVWPEE80 pKa = 3.71QMQQALIHH88 pKa = 5.08VQSYY92 pKa = 10.27KK93 pKa = 10.28PDD95 pKa = 3.36NRR97 pKa = 11.84PIAGPNLRR105 pKa = 11.84DD106 pKa = 3.53SLNRR110 pKa = 11.84QHH112 pKa = 6.96NLPLKK117 pKa = 10.3KK118 pKa = 10.49

Molecular weight:
13.35 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4726

0

4726

1649456

14

3193

349.0

39.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.224 ± 0.035

0.738 ± 0.012

4.961 ± 0.027

6.312 ± 0.041

4.641 ± 0.025

6.921 ± 0.034

2.059 ± 0.019

5.983 ± 0.033

5.954 ± 0.037

10.079 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.016

4.888 ± 0.038

4.155 ± 0.023

4.434 ± 0.026

4.571 ± 0.026

6.006 ± 0.028

5.86 ± 0.037

6.826 ± 0.026

1.204 ± 0.016

3.912 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski