Stereum hirsutum (strain FP-91666) (White-rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Russulales; Stereaceae; Stereum; Stereum hirsutum

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1613 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7RZP5|R7RZP5_STEHR Uncharacterized protein OS=Stereum hirsutum (strain FP-91666) OX=721885 GN=STEHIDRAFT_116270 PE=4 SV=1
MM1 pKa = 7.62CSDD4 pKa = 5.18PIEE7 pKa = 4.42ACRR10 pKa = 11.84CTKK13 pKa = 10.24PAVQYY18 pKa = 7.9PPRR21 pKa = 11.84YY22 pKa = 9.0IVEE25 pKa = 4.11QVQEE29 pKa = 4.28DD30 pKa = 4.3LEE32 pKa = 4.36EE33 pKa = 4.53LCAHH37 pKa = 7.21PSCFYY42 pKa = 10.85CAIDD46 pKa = 3.57GCKK49 pKa = 9.63RR50 pKa = 11.84CCRR53 pKa = 11.84SLQPVQGDD61 pKa = 3.43EE62 pKa = 4.56AGQDD66 pKa = 3.61DD67 pKa = 5.69DD68 pKa = 6.7SNDD71 pKa = 3.61DD72 pKa = 3.9TNDD75 pKa = 3.6SEE77 pKa = 6.16DD78 pKa = 3.95SDD80 pKa = 4.92DD81 pKa = 3.96VQHH84 pKa = 7.29GGLDD88 pKa = 3.7DD89 pKa = 5.2DD90 pKa = 4.94GSGFVKK96 pKa = 10.9YY97 pKa = 10.55EE98 pKa = 3.57EE99 pKa = 4.71ALFDD103 pKa = 4.44DD104 pKa = 4.58RR105 pKa = 11.84SNYY108 pKa = 10.1GDD110 pKa = 4.79LRR112 pKa = 11.84DD113 pKa = 4.59DD114 pKa = 4.42DD115 pKa = 5.17SSVGVKK121 pKa = 10.44DD122 pKa = 3.62NYY124 pKa = 11.28NDD126 pKa = 3.49VTSDD130 pKa = 3.52EE131 pKa = 4.27GDD133 pKa = 3.39EE134 pKa = 4.25MAVRR138 pKa = 11.84APSPSFSDD146 pKa = 3.22

Molecular weight: 16.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7RWR8|R7RWR8_STEHR Uncharacterized protein OS=Stereum hirsutum (strain FP-91666) OX=721885 GN=STEHIDRAFT_69813 PE=4 SV=1
CC1 pKa = 7.2LNCGVTHH8 pKa = 6.31TPLWRR13 pKa = 11.84KK14 pKa = 9.19GLNNEE19 pKa = 4.42LNCNAGGLYY28 pKa = 9.91CQSAYY33 pKa = 10.78LSPRR37 pKa = 11.84LHH39 pKa = 6.54HH40 pKa = 5.42VTNRR44 pKa = 11.84LQFLHH49 pKa = 6.62FFISPAVTAKK59 pKa = 10.47CYY61 pKa = 10.66NCNTTTTPPWRR72 pKa = 11.84KK73 pKa = 9.63DD74 pKa = 3.51DD75 pKa = 3.96EE76 pKa = 4.77GKK78 pKa = 8.32TVCNVCGLYY87 pKa = 10.43FKK89 pKa = 10.27LHH91 pKa = 6.5RR92 pKa = 11.84SPRR95 pKa = 11.84LISMKK100 pKa = 10.22SDD102 pKa = 3.86VIRR105 pKa = 11.84KK106 pKa = 9.32RR107 pKa = 11.84SRR109 pKa = 11.84HH110 pKa = 4.18EE111 pKa = 3.51ARR113 pKa = 11.84RR114 pKa = 11.84ASISSRR120 pKa = 11.84NAPHH124 pKa = 6.61TPSASPGTSRR134 pKa = 11.84RR135 pKa = 11.84TSPTSGQDD143 pKa = 3.06GTNNSNSLSPQSSPPIRR160 pKa = 11.84PRR162 pKa = 11.84RR163 pKa = 3.61

Molecular weight: 18.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1613

0

1613

702429

49

3067

435.5

48.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.317 ± 0.052

1.321 ± 0.023

5.683 ± 0.049

5.979 ± 0.061

3.625 ± 0.036

6.742 ± 0.083

2.697 ± 0.029

4.713 ± 0.047

4.134 ± 0.05

8.891 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.021

3.36 ± 0.031

6.578 ± 0.078

3.506 ± 0.039

6.324 ± 0.05

9.202 ± 0.076

6.314 ± 0.043

6.332 ± 0.048

1.533 ± 0.024

2.626 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski