Amino acid dipepetide frequency for Psychrobacillus sp. OK028

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.593AlaAla: 5.593 ± 0.102
0.49AlaCys: 0.49 ± 0.022
3.35AlaAsp: 3.35 ± 0.061
4.59AlaGlu: 4.59 ± 0.066
3.354AlaPhe: 3.354 ± 0.073
5.147AlaGly: 5.147 ± 0.092
1.268AlaHis: 1.268 ± 0.04
6.47AlaIle: 6.47 ± 0.077
4.863AlaLys: 4.863 ± 0.072
7.066AlaLeu: 7.066 ± 0.099
1.984AlaMet: 1.984 ± 0.045
2.956AlaAsn: 2.956 ± 0.05
2.033AlaPro: 2.033 ± 0.05
2.248AlaGln: 2.248 ± 0.048
2.366AlaArg: 2.366 ± 0.048
4.236AlaSer: 4.236 ± 0.059
3.921AlaThr: 3.921 ± 0.076
5.133AlaVal: 5.133 ± 0.074
0.582AlaTrp: 0.582 ± 0.027
2.313AlaTyr: 2.313 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.396CysAla: 0.396 ± 0.018
0.065CysCys: 0.065 ± 0.009
0.315CysAsp: 0.315 ± 0.019
0.388CysGlu: 0.388 ± 0.021
0.277CysPhe: 0.277 ± 0.015
0.562CysGly: 0.562 ± 0.027
0.16CysHis: 0.16 ± 0.012
0.519CysIle: 0.519 ± 0.026
0.316CysLys: 0.316 ± 0.017
0.549CysLeu: 0.549 ± 0.022
0.136CysMet: 0.136 ± 0.014
0.27CysAsn: 0.27 ± 0.016
0.293CysPro: 0.293 ± 0.017
0.176CysGln: 0.176 ± 0.012
0.236CysArg: 0.236 ± 0.013
0.438CysSer: 0.438 ± 0.019
0.374CysThr: 0.374 ± 0.018
0.354CysVal: 0.354 ± 0.019
0.061CysTrp: 0.061 ± 0.007
0.191CysTyr: 0.191 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.499AspAla: 3.499 ± 0.063
0.296AspCys: 0.296 ± 0.019
2.378AspAsp: 2.378 ± 0.053
4.427AspGlu: 4.427 ± 0.082
2.554AspPhe: 2.554 ± 0.05
3.383AspGly: 3.383 ± 0.067
1.023AspHis: 1.023 ± 0.031
4.25AspIle: 4.25 ± 0.073
3.097AspLys: 3.097 ± 0.049
4.82AspLeu: 4.82 ± 0.074
1.316AspMet: 1.316 ± 0.035
2.005AspAsn: 2.005 ± 0.046
1.808AspPro: 1.808 ± 0.044
1.783AspGln: 1.783 ± 0.048
1.952AspArg: 1.952 ± 0.049
2.815AspSer: 2.815 ± 0.054
2.545AspThr: 2.545 ± 0.053
3.781AspVal: 3.781 ± 0.068
0.569AspTrp: 0.569 ± 0.021
2.132AspTyr: 2.132 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
5.36GluAla: 5.36 ± 0.081
0.339GluCys: 0.339 ± 0.019
3.831GluAsp: 3.831 ± 0.068
7.117GluGlu: 7.117 ± 0.129
2.71GluPhe: 2.71 ± 0.055
4.236GluGly: 4.236 ± 0.071
1.458GluHis: 1.458 ± 0.038
5.959GluIle: 5.959 ± 0.08
6.454GluLys: 6.454 ± 0.102
7.3GluLeu: 7.3 ± 0.095
2.297GluMet: 2.297 ± 0.042
3.904GluAsn: 3.904 ± 0.065
1.943GluPro: 1.943 ± 0.046
3.64GluGln: 3.64 ± 0.075
3.082GluArg: 3.082 ± 0.065
3.738GluSer: 3.738 ± 0.065
4.08GluThr: 4.08 ± 0.065
5.365GluVal: 5.365 ± 0.074
0.902GluTrp: 0.902 ± 0.029
2.249GluTyr: 2.249 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
3.062PheAla: 3.062 ± 0.058
0.292PheCys: 0.292 ± 0.016
2.355PheAsp: 2.355 ± 0.048
3.172PheGlu: 3.172 ± 0.055
2.282PhePhe: 2.282 ± 0.061
3.427PheGly: 3.427 ± 0.066
0.979PheHis: 0.979 ± 0.029
3.941PheIle: 3.941 ± 0.065
2.439PheLys: 2.439 ± 0.049
4.566PheLeu: 4.566 ± 0.074
1.142PheMet: 1.142 ± 0.035
1.936PheAsn: 1.936 ± 0.043
1.706PhePro: 1.706 ± 0.039
1.69PheGln: 1.69 ± 0.043
1.408PheArg: 1.408 ± 0.04
3.415PheSer: 3.415 ± 0.062
2.793PheThr: 2.793 ± 0.058
3.457PheVal: 3.457 ± 0.058
0.481PheTrp: 0.481 ± 0.024
1.701PheTyr: 1.701 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.844GlyAla: 4.844 ± 0.091
0.527GlyCys: 0.527 ± 0.023
3.257GlyAsp: 3.257 ± 0.059
4.464GlyGlu: 4.464 ± 0.068
3.317GlyPhe: 3.317 ± 0.055
4.649GlyGly: 4.649 ± 0.096
1.309GlyHis: 1.309 ± 0.037
6.105GlyIle: 6.105 ± 0.087
4.834GlyLys: 4.834 ± 0.067
6.276GlyLeu: 6.276 ± 0.087
2.062GlyMet: 2.062 ± 0.05
2.867GlyAsn: 2.867 ± 0.055
1.687GlyPro: 1.687 ± 0.044
2.15GlyGln: 2.15 ± 0.053
2.394GlyArg: 2.394 ± 0.056
3.884GlySer: 3.884 ± 0.058
4.13GlyThr: 4.13 ± 0.072
4.947GlyVal: 4.947 ± 0.076
0.74GlyTrp: 0.74 ± 0.025
2.772GlyTyr: 2.772 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.333HisAla: 1.333 ± 0.041
0.155HisCys: 0.155 ± 0.012
0.843HisAsp: 0.843 ± 0.028
1.355HisGlu: 1.355 ± 0.035
1.035HisPhe: 1.035 ± 0.034
1.294HisGly: 1.294 ± 0.038
0.547HisHis: 0.547 ± 0.025
1.584HisIle: 1.584 ± 0.037
1.013HisLys: 1.013 ± 0.033
2.015HisLeu: 2.015 ± 0.046
0.529HisMet: 0.529 ± 0.021
0.785HisAsn: 0.785 ± 0.029
1.057HisPro: 1.057 ± 0.034
0.748HisGln: 0.748 ± 0.032
0.75HisArg: 0.75 ± 0.029
1.163HisSer: 1.163 ± 0.032
1.038HisThr: 1.038 ± 0.031
1.323HisVal: 1.323 ± 0.041
0.193HisTrp: 0.193 ± 0.014
0.808HisTyr: 0.808 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.465IleAla: 6.465 ± 0.078
0.601IleCys: 0.601 ± 0.022
4.492IleAsp: 4.492 ± 0.063
6.199IleGlu: 6.199 ± 0.092
3.491IlePhe: 3.491 ± 0.075
6.148IleGly: 6.148 ± 0.08
1.741IleHis: 1.741 ± 0.037
6.777IleIle: 6.777 ± 0.105
4.452IleLys: 4.452 ± 0.067
7.552IleLeu: 7.552 ± 0.112
1.923IleMet: 1.923 ± 0.043
3.403IleAsn: 3.403 ± 0.056
3.43IlePro: 3.43 ± 0.061
3.173IleGln: 3.173 ± 0.053
3.036IleArg: 3.036 ± 0.048
5.468IleSer: 5.468 ± 0.071
4.706IleThr: 4.706 ± 0.066
6.147IleVal: 6.147 ± 0.084
0.661IleTrp: 0.661 ± 0.026
2.604IleTyr: 2.604 ± 0.049
0.0IleXaa: 0.0 ± 0.0
Lys
4.369LysAla: 4.369 ± 0.072
0.281LysCys: 0.281 ± 0.016
3.765LysAsp: 3.765 ± 0.067
6.663LysGlu: 6.663 ± 0.089
2.232LysPhe: 2.232 ± 0.043
4.24LysGly: 4.24 ± 0.065
1.158LysHis: 1.158 ± 0.03
4.818LysIle: 4.818 ± 0.056
5.598LysLys: 5.598 ± 0.086
5.96LysLeu: 5.96 ± 0.081
2.254LysMet: 2.254 ± 0.048
3.423LysAsn: 3.423 ± 0.063
2.096LysPro: 2.096 ± 0.046
2.958LysGln: 2.958 ± 0.062
2.931LysArg: 2.931 ± 0.062
3.776LysSer: 3.776 ± 0.061
3.741LysThr: 3.741 ± 0.067
4.875LysVal: 4.875 ± 0.07
0.837LysTrp: 0.837 ± 0.028
2.222LysTyr: 2.222 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
7.241LeuAla: 7.241 ± 0.086
0.571LeuCys: 0.571 ± 0.024
4.683LeuAsp: 4.683 ± 0.075
7.002LeuGlu: 7.002 ± 0.099
4.908LeuPhe: 4.908 ± 0.079
6.232LeuGly: 6.232 ± 0.096
1.907LeuHis: 1.907 ± 0.049
7.414LeuIle: 7.414 ± 0.102
6.396LeuLys: 6.396 ± 0.08
10.252LeuLeu: 10.252 ± 0.143
2.614LeuMet: 2.614 ± 0.053
4.362LeuAsn: 4.362 ± 0.059
3.836LeuPro: 3.836 ± 0.059
3.712LeuGln: 3.712 ± 0.068
3.333LeuArg: 3.333 ± 0.063
6.731LeuSer: 6.731 ± 0.091
5.624LeuThr: 5.624 ± 0.069
6.544LeuVal: 6.544 ± 0.08
0.786LeuTrp: 0.786 ± 0.032
3.089LeuTyr: 3.089 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
1.848MetAla: 1.848 ± 0.044
0.136MetCys: 0.136 ± 0.011
1.563MetAsp: 1.563 ± 0.039
2.097MetGlu: 2.097 ± 0.044
1.066MetPhe: 1.066 ± 0.03
1.705MetGly: 1.705 ± 0.042
0.473MetHis: 0.473 ± 0.022
2.144MetIle: 2.144 ± 0.044
2.561MetLys: 2.561 ± 0.05
2.582MetLeu: 2.582 ± 0.054
0.785MetMet: 0.785 ± 0.026
1.57MetAsn: 1.57 ± 0.037
0.963MetPro: 0.963 ± 0.032
0.972MetGln: 0.972 ± 0.031
1.04MetArg: 1.04 ± 0.034
1.737MetSer: 1.737 ± 0.039
1.823MetThr: 1.823 ± 0.041
1.621MetVal: 1.621 ± 0.04
0.203MetTrp: 0.203 ± 0.014
0.775MetTyr: 0.775 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.033AsnAla: 3.033 ± 0.064
0.294AsnCys: 0.294 ± 0.018
2.368AsnAsp: 2.368 ± 0.047
3.702AsnGlu: 3.702 ± 0.064
1.84AsnPhe: 1.84 ± 0.04
3.392AsnGly: 3.392 ± 0.068
0.95AsnHis: 0.95 ± 0.028
3.559AsnIle: 3.559 ± 0.061
3.105AsnLys: 3.105 ± 0.054
4.113AsnLeu: 4.113 ± 0.064
1.237AsnMet: 1.237 ± 0.035
2.444AsnAsn: 2.444 ± 0.059
2.14AsnPro: 2.14 ± 0.045
1.601AsnGln: 1.601 ± 0.043
1.972AsnArg: 1.972 ± 0.043
2.741AsnSer: 2.741 ± 0.054
2.388AsnThr: 2.388 ± 0.043
3.225AsnVal: 3.225 ± 0.049
0.533AsnTrp: 0.533 ± 0.024
1.627AsnTyr: 1.627 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
2.185ProAla: 2.185 ± 0.057
0.177ProCys: 0.177 ± 0.014
1.744ProAsp: 1.744 ± 0.045
2.718ProGlu: 2.718 ± 0.048
2.023ProPhe: 2.023 ± 0.049
2.152ProGly: 2.152 ± 0.053
0.763ProHis: 0.763 ± 0.028
2.998ProIle: 2.998 ± 0.054
2.204ProLys: 2.204 ± 0.046
3.381ProLeu: 3.381 ± 0.061
0.853ProMet: 0.853 ± 0.028
1.813ProAsn: 1.813 ± 0.042
0.91ProPro: 0.91 ± 0.032
1.071ProGln: 1.071 ± 0.033
0.965ProArg: 0.965 ± 0.033
2.274ProSer: 2.274 ± 0.045
2.226ProThr: 2.226 ± 0.045
2.767ProVal: 2.767 ± 0.061
0.336ProTrp: 0.336 ± 0.017
1.345ProTyr: 1.345 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.742GlnAla: 2.742 ± 0.054
0.168GlnCys: 0.168 ± 0.011
1.624GlnAsp: 1.624 ± 0.04
2.707GlnGlu: 2.707 ± 0.057
1.609GlnPhe: 1.609 ± 0.038
2.038GlnGly: 2.038 ± 0.05
0.734GlnHis: 0.734 ± 0.027
2.694GlnIle: 2.694 ± 0.051
2.6GlnLys: 2.6 ± 0.058
4.107GlnLeu: 4.107 ± 0.076
1.161GlnMet: 1.161 ± 0.037
1.675GlnAsn: 1.675 ± 0.043
1.115GlnPro: 1.115 ± 0.036
1.88GlnGln: 1.88 ± 0.048
1.339GlnArg: 1.339 ± 0.039
2.166GlnSer: 2.166 ± 0.047
2.066GlnThr: 2.066 ± 0.043
2.553GlnVal: 2.553 ± 0.046
0.367GlnTrp: 0.367 ± 0.019
1.178GlnTyr: 1.178 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
2.356ArgAla: 2.356 ± 0.051
0.204ArgCys: 0.204 ± 0.015
1.862ArgAsp: 1.862 ± 0.044
2.809ArgGlu: 2.809 ± 0.054
1.726ArgPhe: 1.726 ± 0.04
2.174ArgGly: 2.174 ± 0.041
0.691ArgHis: 0.691 ± 0.026
2.989ArgIle: 2.989 ± 0.054
2.933ArgLys: 2.933 ± 0.048
3.688ArgLeu: 3.688 ± 0.07
1.203ArgMet: 1.203 ± 0.033
1.77ArgAsn: 1.77 ± 0.047
1.195ArgPro: 1.195 ± 0.036
1.345ArgGln: 1.345 ± 0.036
1.491ArgArg: 1.491 ± 0.046
2.017ArgSer: 2.017 ± 0.046
1.88ArgThr: 1.88 ± 0.044
2.505ArgVal: 2.505 ± 0.046
0.352ArgTrp: 0.352 ± 0.016
1.328ArgTyr: 1.328 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
3.783SerAla: 3.783 ± 0.061
0.341SerCys: 0.341 ± 0.018
2.911SerAsp: 2.911 ± 0.05
4.149SerGlu: 4.149 ± 0.06
3.416SerPhe: 3.416 ± 0.06
4.374SerGly: 4.374 ± 0.067
1.176SerHis: 1.176 ± 0.033
5.764SerIle: 5.764 ± 0.081
4.056SerLys: 4.056 ± 0.061
6.24SerLeu: 6.24 ± 0.086
1.786SerMet: 1.786 ± 0.038
2.876SerAsn: 2.876 ± 0.062
2.199SerPro: 2.199 ± 0.048
1.86SerGln: 1.86 ± 0.036
2.085SerArg: 2.085 ± 0.052
4.129SerSer: 4.129 ± 0.07
3.533SerThr: 3.533 ± 0.06
4.33SerVal: 4.33 ± 0.07
0.585SerTrp: 0.585 ± 0.024
2.235SerTyr: 2.235 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
4.02ThrAla: 4.02 ± 0.077
0.292ThrCys: 0.292 ± 0.017
2.898ThrAsp: 2.898 ± 0.055
3.912ThrGlu: 3.912 ± 0.077
2.804ThrPhe: 2.804 ± 0.057
4.259ThrGly: 4.259 ± 0.071
1.094ThrHis: 1.094 ± 0.033
4.932ThrIle: 4.932 ± 0.067
3.738ThrLys: 3.738 ± 0.06
5.371ThrLeu: 5.371 ± 0.074
1.38ThrMet: 1.38 ± 0.04
2.782ThrAsn: 2.782 ± 0.054
2.309ThrPro: 2.309 ± 0.043
1.559ThrGln: 1.559 ± 0.036
1.817ThrArg: 1.817 ± 0.046
3.563ThrSer: 3.563 ± 0.054
3.457ThrThr: 3.457 ± 0.076
4.284ThrVal: 4.284 ± 0.084
0.525ThrTrp: 0.525 ± 0.025
2.101ThrTyr: 2.101 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
5.032ValAla: 5.032 ± 0.071
0.509ValCys: 0.509 ± 0.024
3.736ValAsp: 3.736 ± 0.056
5.262ValGlu: 5.262 ± 0.083
3.229ValPhe: 3.229 ± 0.057
4.806ValGly: 4.806 ± 0.072
1.299ValHis: 1.299 ± 0.035
5.959ValIle: 5.959 ± 0.085
4.614ValLys: 4.614 ± 0.072
7.009ValLeu: 7.009 ± 0.074
1.776ValMet: 1.776 ± 0.046
3.313ValAsn: 3.313 ± 0.056
2.621ValPro: 2.621 ± 0.05
2.427ValGln: 2.427 ± 0.047
2.556ValArg: 2.556 ± 0.056
4.676ValSer: 4.676 ± 0.066
4.278ValThr: 4.278 ± 0.086
5.288ValVal: 5.288 ± 0.088
0.635ValTrp: 0.635 ± 0.027
2.254ValTyr: 2.254 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.558TrpAla: 0.558 ± 0.029
0.068TrpCys: 0.068 ± 0.008
0.537TrpAsp: 0.537 ± 0.024
0.67TrpGlu: 0.67 ± 0.027
0.479TrpPhe: 0.479 ± 0.023
0.61TrpGly: 0.61 ± 0.026
0.198TrpHis: 0.198 ± 0.015
0.906TrpIle: 0.906 ± 0.032
0.76TrpLys: 0.76 ± 0.03
1.03TrpLeu: 1.03 ± 0.036
0.328TrpMet: 0.328 ± 0.017
0.557TrpAsn: 0.557 ± 0.022
0.248TrpPro: 0.248 ± 0.014
0.338TrpGln: 0.338 ± 0.019
0.349TrpArg: 0.349 ± 0.019
0.659TrpSer: 0.659 ± 0.024
0.569TrpThr: 0.569 ± 0.024
0.569TrpVal: 0.569 ± 0.026
0.125TrpTrp: 0.125 ± 0.011
0.324TrpTyr: 0.324 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.244TyrAla: 2.244 ± 0.041
0.27TyrCys: 0.27 ± 0.015
1.918TyrAsp: 1.918 ± 0.047
2.561TyrGlu: 2.561 ± 0.061
1.892TyrPhe: 1.892 ± 0.046
2.388TyrGly: 2.388 ± 0.05
0.645TyrHis: 0.645 ± 0.024
2.691TyrIle: 2.691 ± 0.052
2.162TyrLys: 2.162 ± 0.049
3.361TyrLeu: 3.361 ± 0.061
0.895TyrMet: 0.895 ± 0.03
1.551TyrAsn: 1.551 ± 0.038
1.315TyrPro: 1.315 ± 0.033
1.189TyrGln: 1.189 ± 0.038
1.419TyrArg: 1.419 ± 0.037
2.2TyrSer: 2.2 ± 0.045
1.928TyrThr: 1.928 ± 0.045
2.209TyrVal: 2.209 ± 0.042
0.408TyrTrp: 0.408 ± 0.018
1.429TyrTyr: 1.429 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3657 proteins (1065806 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski