Clostridium sp. CAG:299

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2441 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7ABW3|R7ABW3_9CLOT Heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase OS=Clostridium sp. CAG:299 OX=1262792 GN=BN593_01385 PE=3 SV=1
MM1 pKa = 7.58EE2 pKa = 5.15EE3 pKa = 4.45KK4 pKa = 10.51KK5 pKa = 10.7QSGWSNLNGISDD17 pKa = 4.12DD18 pKa = 5.59DD19 pKa = 4.21KK20 pKa = 11.34TCGLNDD26 pKa = 3.81MNCLGEE32 pKa = 6.16DD33 pKa = 3.97DD34 pKa = 6.14DD35 pKa = 5.91SSCGLNDD42 pKa = 3.56MNCVDD47 pKa = 4.68GKK49 pKa = 10.93DD50 pKa = 4.79DD51 pKa = 4.17MSCSLNNMNCEE62 pKa = 4.03EE63 pKa = 4.35EE64 pKa = 4.2QNKK67 pKa = 9.74

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6ZDW3|R6ZDW3_9CLOT Tex-like protein N-terminal domain protein OS=Clostridium sp. CAG:299 OX=1262792 GN=BN593_02337 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.44VHH16 pKa = 5.57GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 9.64LTAA44 pKa = 4.21

Molecular weight: 4.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2441

0

2441

749053

29

2427

306.9

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.291 ± 0.056

1.494 ± 0.02

5.256 ± 0.037

8.213 ± 0.065

4.147 ± 0.038

7.737 ± 0.048

1.673 ± 0.022

6.579 ± 0.045

6.162 ± 0.046

9.359 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.138 ± 0.026

3.609 ± 0.028

3.558 ± 0.027

3.327 ± 0.03

5.19 ± 0.047

5.901 ± 0.041

5.066 ± 0.029

6.709 ± 0.04

0.919 ± 0.019

3.658 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski