Amino acid dipepetide frequency for Microbacterium phage KaiHaiDragon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.741AlaAla: 16.741 ± 1.502
0.71AlaCys: 0.71 ± 0.215
8.222AlaAsp: 8.222 ± 0.803
7.69AlaGlu: 7.69 ± 0.713
3.076AlaPhe: 3.076 ± 0.344
8.991AlaGly: 8.991 ± 0.889
2.544AlaHis: 2.544 ± 0.435
5.383AlaIle: 5.383 ± 0.683
3.431AlaLys: 3.431 ± 0.502
11.713AlaLeu: 11.713 ± 1.27
3.372AlaMet: 3.372 ± 0.419
3.135AlaAsn: 3.135 ± 0.388
6.152AlaPro: 6.152 ± 0.546
4.732AlaGln: 4.732 ± 0.56
7.394AlaArg: 7.394 ± 0.993
6.98AlaSer: 6.98 ± 0.693
7.927AlaThr: 7.927 ± 0.666
7.098AlaVal: 7.098 ± 0.688
2.839AlaTrp: 2.839 ± 0.38
2.662AlaTyr: 2.662 ± 0.393
0.0AlaXaa: 0.0 ± 0.0
Cys
0.71CysAla: 0.71 ± 0.219
0.177CysCys: 0.177 ± 0.148
1.124CysAsp: 1.124 ± 0.29
0.651CysGlu: 0.651 ± 0.185
0.237CysPhe: 0.237 ± 0.103
1.361CysGly: 1.361 ± 0.326
0.296CysHis: 0.296 ± 0.119
0.237CysIle: 0.237 ± 0.114
0.0CysLys: 0.0 ± 0.0
0.296CysLeu: 0.296 ± 0.13
0.177CysMet: 0.177 ± 0.109
0.177CysAsn: 0.177 ± 0.101
0.651CysPro: 0.651 ± 0.205
0.118CysGln: 0.118 ± 0.076
0.651CysArg: 0.651 ± 0.166
0.473CysSer: 0.473 ± 0.242
0.296CysThr: 0.296 ± 0.117
0.592CysVal: 0.592 ± 0.208
0.118CysTrp: 0.118 ± 0.084
0.177CysTyr: 0.177 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
8.577AspAla: 8.577 ± 0.947
0.651AspCys: 0.651 ± 0.256
5.206AspAsp: 5.206 ± 0.693
4.791AspGlu: 4.791 ± 0.694
1.834AspPhe: 1.834 ± 0.265
6.684AspGly: 6.684 ± 0.589
1.361AspHis: 1.361 ± 0.306
3.017AspIle: 3.017 ± 0.423
1.006AspLys: 1.006 ± 0.224
5.856AspLeu: 5.856 ± 0.545
1.538AspMet: 1.538 ± 0.308
1.42AspAsn: 1.42 ± 0.295
4.141AspPro: 4.141 ± 0.523
2.307AspGln: 2.307 ± 0.297
5.265AspArg: 5.265 ± 0.668
3.372AspSer: 3.372 ± 0.506
2.839AspThr: 2.839 ± 0.459
4.851AspVal: 4.851 ± 0.641
1.006AspTrp: 1.006 ± 0.281
1.715AspTyr: 1.715 ± 0.388
0.0AspXaa: 0.0 ± 0.0
Glu
8.636GluAla: 8.636 ± 0.967
0.473GluCys: 0.473 ± 0.154
5.087GluAsp: 5.087 ± 0.684
3.668GluGlu: 3.668 ± 0.554
1.538GluPhe: 1.538 ± 0.315
5.146GluGly: 5.146 ± 0.63
1.597GluHis: 1.597 ± 0.32
1.952GluIle: 1.952 ± 0.458
2.07GluLys: 2.07 ± 0.358
3.135GluLeu: 3.135 ± 0.403
1.301GluMet: 1.301 ± 0.231
1.42GluAsn: 1.42 ± 0.28
5.146GluPro: 5.146 ± 0.75
2.189GluGln: 2.189 ± 0.334
5.915GluArg: 5.915 ± 0.721
2.899GluSer: 2.899 ± 0.457
3.549GluThr: 3.549 ± 0.573
5.856GluVal: 5.856 ± 0.678
1.301GluTrp: 1.301 ± 0.279
2.011GluTyr: 2.011 ± 0.335
0.0GluXaa: 0.0 ± 0.0
Phe
3.076PheAla: 3.076 ± 0.431
0.237PheCys: 0.237 ± 0.135
2.13PheAsp: 2.13 ± 0.379
1.834PheGlu: 1.834 ± 0.303
0.71PhePhe: 0.71 ± 0.189
2.662PheGly: 2.662 ± 0.447
0.355PheHis: 0.355 ± 0.124
1.301PheIle: 1.301 ± 0.272
0.828PheLys: 0.828 ± 0.222
1.597PheLeu: 1.597 ± 0.321
0.532PheMet: 0.532 ± 0.173
0.355PheAsn: 0.355 ± 0.153
1.301PhePro: 1.301 ± 0.286
0.473PheGln: 0.473 ± 0.214
1.361PheArg: 1.361 ± 0.299
1.242PheSer: 1.242 ± 0.262
2.07PheThr: 2.07 ± 0.332
1.597PheVal: 1.597 ± 0.302
0.177PheTrp: 0.177 ± 0.081
0.71PheTyr: 0.71 ± 0.195
0.0PheXaa: 0.0 ± 0.0
Gly
8.045GlyAla: 8.045 ± 0.864
0.71GlyCys: 0.71 ± 0.197
6.27GlyAsp: 6.27 ± 0.685
5.56GlyGlu: 5.56 ± 0.486
2.78GlyPhe: 2.78 ± 0.448
7.276GlyGly: 7.276 ± 0.64
2.07GlyHis: 2.07 ± 0.466
4.437GlyIle: 4.437 ± 0.626
2.544GlyLys: 2.544 ± 0.382
6.625GlyLeu: 6.625 ± 1.048
3.372GlyMet: 3.372 ± 0.478
2.07GlyAsn: 2.07 ± 0.366
3.372GlyPro: 3.372 ± 0.444
3.253GlyGln: 3.253 ± 0.414
6.034GlyArg: 6.034 ± 0.576
5.206GlySer: 5.206 ± 0.587
6.211GlyThr: 6.211 ± 0.594
6.211GlyVal: 6.211 ± 0.536
2.484GlyTrp: 2.484 ± 0.34
2.839GlyTyr: 2.839 ± 0.377
0.0GlyXaa: 0.0 ± 0.0
His
2.07HisAla: 2.07 ± 0.323
0.177HisCys: 0.177 ± 0.105
1.361HisAsp: 1.361 ± 0.264
1.301HisGlu: 1.301 ± 0.284
0.473HisPhe: 0.473 ± 0.17
2.189HisGly: 2.189 ± 0.436
0.473HisHis: 0.473 ± 0.213
0.946HisIle: 0.946 ± 0.267
0.532HisLys: 0.532 ± 0.169
2.13HisLeu: 2.13 ± 0.426
0.355HisMet: 0.355 ± 0.136
0.532HisAsn: 0.532 ± 0.185
1.479HisPro: 1.479 ± 0.307
0.237HisGln: 0.237 ± 0.11
1.124HisArg: 1.124 ± 0.299
0.946HisSer: 0.946 ± 0.198
1.242HisThr: 1.242 ± 0.317
1.42HisVal: 1.42 ± 0.277
0.414HisTrp: 0.414 ± 0.167
0.769HisTyr: 0.769 ± 0.231
0.0HisXaa: 0.0 ± 0.0
Ile
4.791IleAla: 4.791 ± 0.444
0.473IleCys: 0.473 ± 0.209
3.313IleAsp: 3.313 ± 0.46
4.082IleGlu: 4.082 ± 0.484
0.828IlePhe: 0.828 ± 0.201
3.49IleGly: 3.49 ± 0.397
0.71IleHis: 0.71 ± 0.196
1.834IleIle: 1.834 ± 0.464
1.124IleLys: 1.124 ± 0.237
2.425IleLeu: 2.425 ± 0.435
1.006IleMet: 1.006 ± 0.315
1.006IleAsn: 1.006 ± 0.284
3.549IlePro: 3.549 ± 0.713
1.242IleGln: 1.242 ± 0.294
3.372IleArg: 3.372 ± 0.528
2.425IleSer: 2.425 ± 0.432
4.318IleThr: 4.318 ± 0.517
4.141IleVal: 4.141 ± 0.436
0.592IleTrp: 0.592 ± 0.172
0.532IleTyr: 0.532 ± 0.147
0.0IleXaa: 0.0 ± 0.0
Lys
3.549LysAla: 3.549 ± 0.504
0.296LysCys: 0.296 ± 0.128
1.006LysAsp: 1.006 ± 0.245
0.651LysGlu: 0.651 ± 0.209
0.769LysPhe: 0.769 ± 0.269
2.189LysGly: 2.189 ± 0.391
0.592LysHis: 0.592 ± 0.194
1.065LysIle: 1.065 ± 0.249
0.651LysLys: 0.651 ± 0.228
1.006LysLeu: 1.006 ± 0.276
0.592LysMet: 0.592 ± 0.185
0.296LysAsn: 0.296 ± 0.129
1.775LysPro: 1.775 ± 0.373
0.592LysGln: 0.592 ± 0.196
2.425LysArg: 2.425 ± 0.513
1.952LysSer: 1.952 ± 0.304
1.479LysThr: 1.479 ± 0.39
3.253LysVal: 3.253 ± 0.485
0.355LysTrp: 0.355 ± 0.136
0.355LysTyr: 0.355 ± 0.135
0.0LysXaa: 0.0 ± 0.0
Leu
10.175LeuAla: 10.175 ± 0.957
0.237LeuCys: 0.237 ± 0.115
4.732LeuAsp: 4.732 ± 0.565
4.2LeuGlu: 4.2 ± 0.591
1.538LeuPhe: 1.538 ± 0.384
6.625LeuGly: 6.625 ± 1.058
1.301LeuHis: 1.301 ± 0.234
4.082LeuIle: 4.082 ± 0.693
0.651LeuLys: 0.651 ± 0.185
6.034LeuLeu: 6.034 ± 0.741
1.834LeuMet: 1.834 ± 0.256
1.775LeuAsn: 1.775 ± 0.356
5.206LeuPro: 5.206 ± 0.617
2.13LeuGln: 2.13 ± 0.315
5.087LeuArg: 5.087 ± 0.604
5.442LeuSer: 5.442 ± 0.624
6.329LeuThr: 6.329 ± 0.659
6.093LeuVal: 6.093 ± 0.69
1.242LeuTrp: 1.242 ± 0.261
1.183LeuTyr: 1.183 ± 0.302
0.0LeuXaa: 0.0 ± 0.0
Met
2.839MetAla: 2.839 ± 0.452
0.177MetCys: 0.177 ± 0.099
1.124MetAsp: 1.124 ± 0.238
0.414MetGlu: 0.414 ± 0.164
0.473MetPhe: 0.473 ± 0.169
2.07MetGly: 2.07 ± 0.358
0.532MetHis: 0.532 ± 0.164
1.775MetIle: 1.775 ± 0.375
0.237MetLys: 0.237 ± 0.115
1.597MetLeu: 1.597 ± 0.317
0.355MetMet: 0.355 ± 0.137
0.71MetAsn: 0.71 ± 0.181
1.183MetPro: 1.183 ± 0.311
0.828MetGln: 0.828 ± 0.237
1.656MetArg: 1.656 ± 0.264
2.603MetSer: 2.603 ± 0.359
2.721MetThr: 2.721 ± 0.373
1.715MetVal: 1.715 ± 0.304
0.177MetTrp: 0.177 ± 0.1
0.769MetTyr: 0.769 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
3.253AsnAla: 3.253 ± 0.446
0.237AsnCys: 0.237 ± 0.12
1.065AsnAsp: 1.065 ± 0.257
1.006AsnGlu: 1.006 ± 0.217
0.355AsnPhe: 0.355 ± 0.169
3.194AsnGly: 3.194 ± 0.64
0.355AsnHis: 0.355 ± 0.16
0.592AsnIle: 0.592 ± 0.199
0.355AsnLys: 0.355 ± 0.153
2.425AsnLeu: 2.425 ± 0.35
0.473AsnMet: 0.473 ± 0.212
0.532AsnAsn: 0.532 ± 0.205
2.484AsnPro: 2.484 ± 0.447
0.473AsnGln: 0.473 ± 0.158
1.952AsnArg: 1.952 ± 0.365
1.656AsnSer: 1.656 ± 0.312
2.307AsnThr: 2.307 ± 0.346
1.479AsnVal: 1.479 ± 0.254
0.237AsnTrp: 0.237 ± 0.115
0.473AsnTyr: 0.473 ± 0.151
0.0AsnXaa: 0.0 ± 0.0
Pro
7.749ProAla: 7.749 ± 0.913
0.71ProCys: 0.71 ± 0.218
4.791ProAsp: 4.791 ± 0.624
5.975ProGlu: 5.975 ± 0.829
1.361ProPhe: 1.361 ± 0.273
5.442ProGly: 5.442 ± 0.517
1.42ProHis: 1.42 ± 0.317
1.656ProIle: 1.656 ± 0.256
1.42ProLys: 1.42 ± 0.276
3.845ProLeu: 3.845 ± 0.476
1.301ProMet: 1.301 ± 0.264
1.775ProAsn: 1.775 ± 0.411
3.786ProPro: 3.786 ± 0.658
1.715ProGln: 1.715 ± 0.525
2.899ProArg: 2.899 ± 0.433
3.608ProSer: 3.608 ± 0.483
4.791ProThr: 4.791 ± 0.754
4.082ProVal: 4.082 ± 0.521
0.828ProTrp: 0.828 ± 0.228
1.597ProTyr: 1.597 ± 0.295
0.0ProXaa: 0.0 ± 0.0
Gln
3.431GlnAla: 3.431 ± 0.559
0.355GlnCys: 0.355 ± 0.171
1.952GlnAsp: 1.952 ± 0.345
1.538GlnGlu: 1.538 ± 0.265
1.124GlnPhe: 1.124 ± 0.284
2.603GlnGly: 2.603 ± 0.367
0.769GlnHis: 0.769 ± 0.182
1.597GlnIle: 1.597 ± 0.33
0.769GlnLys: 0.769 ± 0.239
1.065GlnLeu: 1.065 ± 0.373
0.946GlnMet: 0.946 ± 0.25
1.183GlnAsn: 1.183 ± 0.269
1.656GlnPro: 1.656 ± 0.345
1.006GlnGln: 1.006 ± 0.248
2.13GlnArg: 2.13 ± 0.4
2.248GlnSer: 2.248 ± 0.374
2.13GlnThr: 2.13 ± 0.314
2.425GlnVal: 2.425 ± 0.381
0.71GlnTrp: 0.71 ± 0.19
1.006GlnTyr: 1.006 ± 0.231
0.0GlnXaa: 0.0 ± 0.0
Arg
7.513ArgAla: 7.513 ± 0.725
0.887ArgCys: 0.887 ± 0.216
4.614ArgAsp: 4.614 ± 0.586
5.442ArgGlu: 5.442 ± 0.683
1.597ArgPhe: 1.597 ± 0.335
5.975ArgGly: 5.975 ± 0.569
1.597ArgHis: 1.597 ± 0.378
2.958ArgIle: 2.958 ± 0.398
2.011ArgLys: 2.011 ± 0.307
6.211ArgLeu: 6.211 ± 0.553
1.656ArgMet: 1.656 ± 0.306
1.361ArgAsn: 1.361 ± 0.297
3.076ArgPro: 3.076 ± 0.418
2.899ArgGln: 2.899 ± 0.486
7.453ArgArg: 7.453 ± 0.919
3.786ArgSer: 3.786 ± 0.432
3.845ArgThr: 3.845 ± 0.579
4.437ArgVal: 4.437 ± 0.549
1.656ArgTrp: 1.656 ± 0.373
2.425ArgTyr: 2.425 ± 0.41
0.0ArgXaa: 0.0 ± 0.0
Ser
8.341SerAla: 8.341 ± 1.127
0.177SerCys: 0.177 ± 0.104
4.377SerAsp: 4.377 ± 0.445
3.963SerGlu: 3.963 ± 0.5
1.597SerPhe: 1.597 ± 0.262
4.91SerGly: 4.91 ± 0.552
1.065SerHis: 1.065 ± 0.236
3.253SerIle: 3.253 ± 0.519
1.656SerLys: 1.656 ± 0.379
4.496SerLeu: 4.496 ± 0.43
1.597SerMet: 1.597 ± 0.31
1.834SerAsn: 1.834 ± 0.345
2.78SerPro: 2.78 ± 0.472
1.183SerGln: 1.183 ± 0.259
3.49SerArg: 3.49 ± 0.422
2.484SerSer: 2.484 ± 0.596
4.377SerThr: 4.377 ± 0.5
4.555SerVal: 4.555 ± 0.504
1.065SerTrp: 1.065 ± 0.245
1.42SerTyr: 1.42 ± 0.395
0.0SerXaa: 0.0 ± 0.0
Thr
7.867ThrAla: 7.867 ± 0.928
0.532ThrCys: 0.532 ± 0.182
3.963ThrAsp: 3.963 ± 0.517
4.673ThrGlu: 4.673 ± 0.567
1.42ThrPhe: 1.42 ± 0.273
6.803ThrGly: 6.803 ± 0.618
1.361ThrHis: 1.361 ± 0.326
3.194ThrIle: 3.194 ± 0.406
2.07ThrLys: 2.07 ± 0.379
6.034ThrLeu: 6.034 ± 0.683
1.065ThrMet: 1.065 ± 0.233
1.361ThrAsn: 1.361 ± 0.266
6.152ThrPro: 6.152 ± 0.649
1.42ThrGln: 1.42 ± 0.281
4.259ThrArg: 4.259 ± 0.498
3.727ThrSer: 3.727 ± 0.442
5.028ThrThr: 5.028 ± 0.573
6.27ThrVal: 6.27 ± 0.626
0.887ThrTrp: 0.887 ± 0.205
2.011ThrTyr: 2.011 ± 0.294
0.0ThrXaa: 0.0 ± 0.0
Val
8.4ValAla: 8.4 ± 0.683
0.769ValCys: 0.769 ± 0.188
3.668ValAsp: 3.668 ± 0.515
4.791ValGlu: 4.791 ± 0.471
1.479ValPhe: 1.479 ± 0.282
6.152ValGly: 6.152 ± 0.587
1.124ValHis: 1.124 ± 0.258
3.963ValIle: 3.963 ± 0.551
2.07ValLys: 2.07 ± 0.386
5.915ValLeu: 5.915 ± 0.615
1.597ValMet: 1.597 ± 0.313
2.662ValAsn: 2.662 ± 0.387
4.969ValPro: 4.969 ± 0.516
2.248ValGln: 2.248 ± 0.467
5.146ValArg: 5.146 ± 0.623
4.851ValSer: 4.851 ± 0.647
6.152ValThr: 6.152 ± 0.469
4.969ValVal: 4.969 ± 0.56
1.715ValTrp: 1.715 ± 0.336
2.13ValTyr: 2.13 ± 0.363
0.0ValXaa: 0.0 ± 0.0
Trp
2.13TrpAla: 2.13 ± 0.388
0.414TrpCys: 0.414 ± 0.15
0.769TrpAsp: 0.769 ± 0.221
1.065TrpGlu: 1.065 ± 0.222
0.946TrpPhe: 0.946 ± 0.236
1.242TrpGly: 1.242 ± 0.278
0.414TrpHis: 0.414 ± 0.131
0.828TrpIle: 0.828 ± 0.231
0.769TrpLys: 0.769 ± 0.221
1.656TrpLeu: 1.656 ± 0.35
0.237TrpMet: 0.237 ± 0.105
0.651TrpAsn: 0.651 ± 0.186
0.71TrpPro: 0.71 ± 0.204
0.592TrpGln: 0.592 ± 0.197
1.656TrpArg: 1.656 ± 0.37
1.183TrpSer: 1.183 ± 0.26
0.769TrpThr: 0.769 ± 0.191
1.715TrpVal: 1.715 ± 0.314
0.532TrpTrp: 0.532 ± 0.176
0.651TrpTyr: 0.651 ± 0.183
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.899TyrAla: 2.899 ± 0.478
0.118TyrCys: 0.118 ± 0.078
2.662TyrAsp: 2.662 ± 0.392
1.597TyrGlu: 1.597 ± 0.322
0.414TyrPhe: 0.414 ± 0.174
2.366TyrGly: 2.366 ± 0.464
0.237TyrHis: 0.237 ± 0.116
1.183TyrIle: 1.183 ± 0.242
0.651TyrLys: 0.651 ± 0.181
1.834TyrLeu: 1.834 ± 0.313
0.592TyrMet: 0.592 ± 0.223
0.651TyrAsn: 0.651 ± 0.17
1.183TyrPro: 1.183 ± 0.274
1.006TyrGln: 1.006 ± 0.216
2.13TyrArg: 2.13 ± 0.314
1.597TyrSer: 1.597 ± 0.364
1.715TyrThr: 1.715 ± 0.341
2.011TyrVal: 2.011 ± 0.323
0.592TyrTrp: 0.592 ± 0.181
0.769TyrTyr: 0.769 ± 0.24
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (16906 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski