CRESS virus sp. ctpVY4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; unclassified Cressdnaviricota; CRESS viruses

Average proteome isoelectric point is 5.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W910|A0A5Q2W910_9VIRU Rep protein OS=CRESS virus sp. ctpVY4 OX=2656689 PE=4 SV=1
MM1 pKa = 7.5EE2 pKa = 6.16CSTSTPGTSTQNTPCLTTGKK22 pKa = 8.3TGPNSTYY29 pKa = 10.48SSNSSEE35 pKa = 4.14HH36 pKa = 6.62SKK38 pKa = 11.07SSTSPTSTDD47 pKa = 3.24ARR49 pKa = 11.84PEE51 pKa = 3.97SDD53 pKa = 3.81GEE55 pKa = 4.4SHH57 pKa = 6.95ASSSPTTYY65 pKa = 10.65LHH67 pKa = 5.97STAHH71 pKa = 5.99TEE73 pKa = 3.91PGAMQTPSWWFSEE86 pKa = 4.11LEE88 pKa = 4.28NIFSEE93 pKa = 4.57

Molecular weight: 9.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W6C8|A0A5Q2W6C8_9VIRU Uncharacterized protein OS=CRESS virus sp. ctpVY4 OX=2656689 PE=4 SV=1
MM1 pKa = 6.84MHH3 pKa = 5.84QPGRR7 pKa = 11.84GGEE10 pKa = 4.1YY11 pKa = 10.29KK12 pKa = 10.45RR13 pKa = 11.84VDD15 pKa = 3.44SNIIVSTLFWGMDD28 pKa = 3.44EE29 pKa = 4.7NVPHH33 pKa = 6.83PLQWNSKK40 pKa = 7.15QFFITWPRR48 pKa = 11.84CPIPAPVALEE58 pKa = 4.3DD59 pKa = 5.08IINILYY65 pKa = 9.68GRR67 pKa = 11.84QRR69 pKa = 11.84QRR71 pKa = 11.84EE72 pKa = 4.24SFHH75 pKa = 7.93IRR77 pKa = 11.84MAQEE81 pKa = 3.66EE82 pKa = 4.36HH83 pKa = 7.67DD84 pKa = 4.48DD85 pKa = 4.37GGLHH89 pKa = 5.92LHH91 pKa = 6.68AFIRR95 pKa = 11.84LDD97 pKa = 2.91KK98 pKa = 10.84ALRR101 pKa = 11.84FRR103 pKa = 11.84NSSLFDD109 pKa = 3.6LQHH112 pKa = 5.37YY113 pKa = 9.28HH114 pKa = 7.21PNIQGVRR121 pKa = 11.84NTVAVLAYY129 pKa = 9.98VSKK132 pKa = 10.87DD133 pKa = 3.0GNYY136 pKa = 9.54VDD138 pKa = 5.02HH139 pKa = 6.69GHH141 pKa = 7.32PEE143 pKa = 3.73PLGYY147 pKa = 9.83LDD149 pKa = 5.61HH150 pKa = 7.67GDD152 pKa = 3.76AQPQGRR158 pKa = 11.84VSKK161 pKa = 10.12WSAIVGAEE169 pKa = 3.9TRR171 pKa = 11.84EE172 pKa = 3.95EE173 pKa = 3.89FMDD176 pKa = 5.2AIKK179 pKa = 9.25TAAPRR184 pKa = 11.84DD185 pKa = 3.82YY186 pKa = 10.96VLSLEE191 pKa = 4.49RR192 pKa = 11.84IKK194 pKa = 10.86FYY196 pKa = 11.13ADD198 pKa = 2.82YY199 pKa = 9.96RR200 pKa = 11.84YY201 pKa = 10.41AVDD204 pKa = 3.65TPIYY208 pKa = 10.29AHH210 pKa = 7.55DD211 pKa = 3.84PTLVFNLPARR221 pKa = 11.84VSPDD225 pKa = 3.14LNRR228 pKa = 11.84WTLQRR233 pKa = 11.84QNVSKK238 pKa = 10.96LNLVII243 pKa = 5.04

Molecular weight: 28.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

431

93

243

143.7

16.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.497 ± 0.774

1.16 ± 0.595

6.729 ± 1.767

5.336 ± 1.153

5.104 ± 1.855

5.8 ± 0.195

3.944 ± 1.0

4.64 ± 1.421

3.48 ± 0.653

7.193 ± 1.486

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.027

4.64 ± 0.236

6.032 ± 1.388

3.944 ± 0.831

5.8 ± 1.879

8.585 ± 5.32

6.729 ± 4.141

5.104 ± 1.921

2.784 ± 0.834

4.408 ± 0.877
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski