Rhodovulum sp. PH10

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum; unclassified Rhodovulum

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4490 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J6UFF0|J6UFF0_9RHOB Uncharacterized protein OS=Rhodovulum sp. PH10 OX=1187851 GN=A33M_2012 PE=4 SV=1
MM1 pKa = 7.35SLSGALSSAVSALNAQSQAISMISDD26 pKa = 4.37NIANASTTGYY36 pKa = 10.15KK37 pKa = 9.92SVSASFEE44 pKa = 4.44SMLSSSTATSSYY56 pKa = 10.15SAGGVSVSTRR66 pKa = 11.84YY67 pKa = 10.18NISEE71 pKa = 3.88QGLRR75 pKa = 11.84TSSSNDD81 pKa = 2.93TAIAIEE87 pKa = 4.39GNGFFCVSDD96 pKa = 3.78GKK98 pKa = 9.33TGSASYY104 pKa = 7.66YY105 pKa = 9.2TRR107 pKa = 11.84AGDD110 pKa = 3.75FTVDD114 pKa = 3.29DD115 pKa = 4.63DD116 pKa = 5.62GYY118 pKa = 11.05LVSNGYY124 pKa = 9.38YY125 pKa = 10.43LLGWRR130 pKa = 11.84TDD132 pKa = 3.1AEE134 pKa = 4.76GNVTSGEE141 pKa = 3.96NSASLEE147 pKa = 4.3RR148 pKa = 11.84IDD150 pKa = 3.85TGTVQSIAKK159 pKa = 8.28ATTTEE164 pKa = 4.19TVEE167 pKa = 4.15ANLPADD173 pKa = 3.8AAVGDD178 pKa = 4.4TFTSTLEE185 pKa = 4.25VYY187 pKa = 10.56DD188 pKa = 4.13SLGTASNVTVTWTKK202 pKa = 8.57TAEE205 pKa = 4.29NAWSATYY212 pKa = 11.01SDD214 pKa = 4.23ATLASDD220 pKa = 3.8PSVVTGTSSGTTTITFDD237 pKa = 3.63GDD239 pKa = 3.52GNLASVTGNTLAIDD253 pKa = 3.82WTTGASDD260 pKa = 3.43SAITIDD266 pKa = 3.15VGTIGGADD274 pKa = 3.82GLTQRR279 pKa = 11.84ASGLATPAVDD289 pKa = 5.39LKK291 pKa = 11.46SIDD294 pKa = 3.83QDD296 pKa = 3.43GLAYY300 pKa = 10.4GSLTGVSIANGGDD313 pKa = 3.28VTATYY318 pKa = 11.25SNGEE322 pKa = 3.9TLVIYY327 pKa = 10.04KK328 pKa = 9.98VAVATFADD336 pKa = 4.37PNGLTTLSGGIYY348 pKa = 9.99EE349 pKa = 4.64EE350 pKa = 4.66SLASGGATLHH360 pKa = 6.06EE361 pKa = 4.93SGVGGAGDD369 pKa = 3.34IYY371 pKa = 10.85GSQLEE376 pKa = 4.54SSTADD381 pKa = 3.17TTEE384 pKa = 4.16EE385 pKa = 4.13FSTMITAQQAYY396 pKa = 9.09SAAAQVVSTVSDD408 pKa = 3.45MYY410 pKa = 10.25DD411 pKa = 3.01TLMSAVRR418 pKa = 3.81

Molecular weight: 42.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J6LK74|J6LK74_9RHOB UDP-N-acetylmuramoylalanine--D-glutamate ligase OS=Rhodovulum sp. PH10 OX=1187851 GN=murD PE=3 SV=1
MM1 pKa = 7.36IVHH4 pKa = 7.29GGRR7 pKa = 11.84PWRR10 pKa = 11.84GAGRR14 pKa = 11.84NARR17 pKa = 11.84GTNGGTGRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84QRR30 pKa = 11.84ARR32 pKa = 11.84RR33 pKa = 11.84AARR36 pKa = 11.84FGGGPLTAA44 pKa = 5.48

Molecular weight: 4.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4490

0

4490

1324287

37

2647

294.9

31.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.718 ± 0.057

0.896 ± 0.012

5.71 ± 0.031

5.648 ± 0.042

3.486 ± 0.026

8.72 ± 0.035

2.046 ± 0.019

4.479 ± 0.026

2.975 ± 0.028

9.899 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.222 ± 0.018

1.995 ± 0.017

5.884 ± 0.033

2.537 ± 0.022

8.052 ± 0.048

4.849 ± 0.024

5.515 ± 0.025

8.166 ± 0.031

1.268 ± 0.015

1.934 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski