Aeromonas phage 25AhydR2PP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Aerosvirus; Aeromonas virus 25AhydR2PP

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1PFQ3|A0A2S1PFQ3_9CAUD Putative tail fiber protein OS=Aeromonas phage 25AhydR2PP OX=2163976 PE=4 SV=1
MM1 pKa = 7.6IPDD4 pKa = 4.26YY5 pKa = 11.27LPDD8 pKa = 4.52CLDD11 pKa = 3.44SVLWLIAPSVTLLQILAYY29 pKa = 10.28GGLTLVAALAIIGSYY44 pKa = 10.97LEE46 pKa = 4.59DD47 pKa = 3.46NQRR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 9.76PP53 pKa = 3.53

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1PFT4|A0A2S1PFT4_9CAUD Putative DNA endonuclease OS=Aeromonas phage 25AhydR2PP OX=2163976 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.33VLNRR6 pKa = 11.84AYY8 pKa = 9.74KK9 pKa = 10.29LKK11 pKa = 10.85ARR13 pKa = 11.84AEE15 pKa = 4.23RR16 pKa = 11.84MLSAMWKK23 pKa = 9.81RR24 pKa = 11.84LHH26 pKa = 6.19KK27 pKa = 10.1WGEE30 pKa = 3.99GYY32 pKa = 9.32EE33 pKa = 4.17RR34 pKa = 11.84KK35 pKa = 10.1HH36 pKa = 4.86IARR39 pKa = 11.84RR40 pKa = 11.84SVVVVLASPLILFEE54 pKa = 4.43MGRR57 pKa = 11.84QAYY60 pKa = 9.16HH61 pKa = 6.78AVAEE65 pKa = 4.69CAAEE69 pKa = 4.48LYY71 pKa = 10.59QYY73 pKa = 10.34IRR75 pKa = 11.84HH76 pKa = 5.93NNN78 pKa = 3.23

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

13215

43

1250

259.1

28.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.898 ± 0.553

1.158 ± 0.163

5.986 ± 0.198

6.599 ± 0.272

3.405 ± 0.184

7.537 ± 0.304

2.217 ± 0.199

4.555 ± 0.275

6.16 ± 0.291

8.468 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.284 ± 0.165

3.731 ± 0.18

3.912 ± 0.205

4.397 ± 0.33

5.728 ± 0.204

6.114 ± 0.36

5.131 ± 0.217

6.977 ± 0.307

1.476 ± 0.127

3.269 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski