Gulosibacter sp. 10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Gulosibacter; unclassified Gulosibacter

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R4FLH9|A0A1R4FLH9_9MICO Hydantoin racemase OS=Gulosibacter sp. 10 OX=1255570 GN=FM112_04910 PE=4 SV=1
MM1 pKa = 7.6NKK3 pKa = 10.12RR4 pKa = 11.84IVSGVALVAAFGLVLSGCARR24 pKa = 11.84ADD26 pKa = 3.5EE27 pKa = 4.67AGGGGVAEE35 pKa = 5.24DD36 pKa = 3.99GGQVEE41 pKa = 4.94HH42 pKa = 7.08LNAAEE47 pKa = 3.94NQMNYY52 pKa = 9.69VDD54 pKa = 4.8EE55 pKa = 6.1DD56 pKa = 4.25GLGHH60 pKa = 7.19GGTLRR65 pKa = 11.84LYY67 pKa = 10.96NNAYY71 pKa = 7.98PANFNFNTVEE81 pKa = 4.23GYY83 pKa = 9.29EE84 pKa = 4.19KK85 pKa = 9.81NTNEE89 pKa = 3.82IMEE92 pKa = 4.49AVWPQLYY99 pKa = 10.3AYY101 pKa = 7.77TADD104 pKa = 3.65GQVIPNDD111 pKa = 4.02LYY113 pKa = 8.86TQRR116 pKa = 11.84IEE118 pKa = 4.3QVSEE122 pKa = 3.84EE123 pKa = 4.07PFAYY127 pKa = 9.91EE128 pKa = 3.67IEE130 pKa = 4.35LVEE133 pKa = 4.26GLTWSDD139 pKa = 3.42GTPLDD144 pKa = 3.49WTSVKK149 pKa = 11.05NNMEE153 pKa = 4.4AYY155 pKa = 10.05QNEE158 pKa = 4.25EE159 pKa = 4.12FNVVSRR165 pKa = 11.84DD166 pKa = 3.32GYY168 pKa = 11.33DD169 pKa = 3.28RR170 pKa = 11.84IEE172 pKa = 4.41EE173 pKa = 4.19VTQGDD178 pKa = 3.94NEE180 pKa = 4.13QTAVITFKK188 pKa = 10.8EE189 pKa = 4.54GEE191 pKa = 4.55VYY193 pKa = 10.9ADD195 pKa = 3.96WIGLSGVMPDD205 pKa = 4.04ALVEE209 pKa = 4.21SAEE212 pKa = 4.14EE213 pKa = 4.63FNTGWVDD220 pKa = 3.83GPKK223 pKa = 8.91VTAGPYY229 pKa = 9.14TIEE232 pKa = 4.04NADD235 pKa = 3.98RR236 pKa = 11.84ANQVVTLVPDD246 pKa = 4.11EE247 pKa = 4.39NWTGARR253 pKa = 11.84DD254 pKa = 3.62AKK256 pKa = 10.63LDD258 pKa = 3.65RR259 pKa = 11.84LSFQTIEE266 pKa = 4.62DD267 pKa = 3.82PSAAATAFNNGQLDD281 pKa = 4.21VIDD284 pKa = 4.95ASAEE288 pKa = 3.98AIYY291 pKa = 10.71SVVIDD296 pKa = 3.89TVDD299 pKa = 3.22NGDD302 pKa = 3.47EE303 pKa = 4.26FEE305 pKa = 4.48IRR307 pKa = 11.84TAAGPDD313 pKa = 3.13WSHH316 pKa = 5.95FTLNGGEE323 pKa = 4.37GNVLSDD329 pKa = 3.77PNLRR333 pKa = 11.84EE334 pKa = 3.85AFFYY338 pKa = 10.71AINRR342 pKa = 11.84ADD344 pKa = 3.81IFMALNGTMPYY355 pKa = 10.28PEE357 pKa = 5.57GIEE360 pKa = 3.84NDD362 pKa = 3.41LLGNHH367 pKa = 6.16MLMSNQDD374 pKa = 3.31GYY376 pKa = 11.22EE377 pKa = 4.07SHH379 pKa = 7.03AGEE382 pKa = 4.99YY383 pKa = 8.76GTGDD387 pKa = 3.42ADD389 pKa = 4.04RR390 pKa = 11.84AKK392 pKa = 10.78EE393 pKa = 4.0LIEE396 pKa = 3.9EE397 pKa = 4.61AGWEE401 pKa = 4.0LGDD404 pKa = 4.68DD405 pKa = 4.02GFYY408 pKa = 10.91QKK410 pKa = 10.77DD411 pKa = 3.92GEE413 pKa = 4.42VLEE416 pKa = 3.96IRR418 pKa = 11.84YY419 pKa = 9.57VYY421 pKa = 10.7NAGSAVNGTIAPIATEE437 pKa = 4.01NLQAAGIKK445 pKa = 9.22LTVEE449 pKa = 3.96QVPPTDD455 pKa = 4.31LFSQYY460 pKa = 10.64VIPGDD465 pKa = 3.51YY466 pKa = 10.39DD467 pKa = 2.9ITMFGWSGNPFVTSSVNSWLSDD489 pKa = 3.39GEE491 pKa = 4.47QNFTNIGSEE500 pKa = 4.17EE501 pKa = 4.57LDD503 pKa = 3.77DD504 pKa = 6.17LLNQLTNEE512 pKa = 4.14ADD514 pKa = 3.23VDD516 pKa = 4.01KK517 pKa = 10.71QTDD520 pKa = 3.92LLNQIDD526 pKa = 3.82EE527 pKa = 4.77ALWGLHH533 pKa = 6.44SNLPLYY539 pKa = 10.34QSYY542 pKa = 11.26DD543 pKa = 4.21FIATPVDD550 pKa = 3.53LANYY554 pKa = 7.41GAPGFANIIDD564 pKa = 3.86WSMVGYY570 pKa = 10.85VDD572 pKa = 5.33GSPHH576 pKa = 7.74LEE578 pKa = 4.09GG579 pKa = 5.32

Molecular weight: 63.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R4G5I3|A0A1R4G5I3_9MICO RNA-binding protein Jag OS=Gulosibacter sp. 10 OX=1255570 GN=FM112_09140 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84KK16 pKa = 8.24HH17 pKa = 4.59GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSSRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 9.71GRR40 pKa = 11.84KK41 pKa = 8.78VLSAA45 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3335

0

3335

1064179

37

1803

319.1

34.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.199 ± 0.06

0.566 ± 0.01

5.818 ± 0.037

7.134 ± 0.046

3.192 ± 0.025

9.259 ± 0.044

1.993 ± 0.02

4.604 ± 0.034

1.819 ± 0.028

10.228 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.91 ± 0.018

1.915 ± 0.023

5.41 ± 0.033

2.823 ± 0.03

7.948 ± 0.057

5.401 ± 0.028

5.283 ± 0.03

8.125 ± 0.034

1.395 ± 0.016

1.98 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski