Vibrio phage VD1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; unclassified Peduovirinae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9TR65|R9TR65_9CAUD Major tail tube protein OS=Vibrio phage VD1 OX=754057 GN=VPPG_00052 PE=4 SV=1
MM1 pKa = 7.53AKK3 pKa = 10.33LYY5 pKa = 7.82RR6 pKa = 11.84TRR8 pKa = 11.84DD9 pKa = 3.03GDD11 pKa = 3.76MLDD14 pKa = 4.35AICYY18 pKa = 9.54SEE20 pKa = 4.72YY21 pKa = 10.23GTEE24 pKa = 3.94QAVTIVLDD32 pKa = 4.22ANPGLAEE39 pKa = 4.24NGAKK43 pKa = 10.47FSAGVQIVLPDD54 pKa = 3.4YY55 pKa = 8.96TPPAEE60 pKa = 4.2EE61 pKa = 5.27DD62 pKa = 3.73EE63 pKa = 4.52DD64 pKa = 4.41VLWSS68 pKa = 3.61

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9TRB3|R9TRB3_9CAUD Uncharacterized protein OS=Vibrio phage VD1 OX=754057 GN=VPPG_00108 PE=4 SV=1
GG1 pKa = 6.64SRR3 pKa = 11.84SRR5 pKa = 11.84LIVEE9 pKa = 3.91INGVISMKK17 pKa = 10.45TFIQKK22 pKa = 9.78HH23 pKa = 4.61ALKK26 pKa = 10.4IGASVSAVVASGVANATVEE45 pKa = 4.19EE46 pKa = 4.39SLNAAVSSGQSNYY59 pKa = 10.44SIVVIGLIGLAAIGFGLRR77 pKa = 11.84AIMGAMRR84 pKa = 4.74

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

117

0

117

24514

53

844

209.5

23.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.277 ± 0.323

1.15 ± 0.12

5.817 ± 0.135

7.106 ± 0.235

3.916 ± 0.164

6.555 ± 0.236

2.027 ± 0.155

5.862 ± 0.166

6.319 ± 0.237

8.518 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.917 ± 0.149

4.516 ± 0.126

3.647 ± 0.137

4.291 ± 0.167

4.74 ± 0.217

6.865 ± 0.207

5.919 ± 0.188

6.694 ± 0.202

1.566 ± 0.095

3.296 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski