Rickenella mellea

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Hymenochaetales; Hymenochaetales incertae sedis; Rickenella

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17053 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y7PJL3|A0A4Y7PJL3_9AGAM Uncharacterized protein OS=Rickenella mellea OX=50990 GN=BD410DRAFT_124353 PE=4 SV=1
MM1 pKa = 6.88VQDD4 pKa = 4.44GPRR7 pKa = 11.84GIQALLQPPDD17 pKa = 4.05DD18 pKa = 5.59PIPTDD23 pKa = 3.37EE24 pKa = 4.25VEE26 pKa = 4.53SYY28 pKa = 11.16GIDD31 pKa = 3.02WAVNDD36 pKa = 5.09DD37 pKa = 4.08PTLMTHH43 pKa = 6.88LLEE46 pKa = 5.86SNPQDD51 pKa = 3.46WDD53 pKa = 3.57DD54 pKa = 3.91QNPFGTPSTPSHH66 pKa = 6.82LSHH69 pKa = 6.94VPCDD73 pKa = 3.73PPNCPFTNQEE83 pKa = 3.97VEE85 pKa = 4.18LLGQLLHH92 pKa = 6.47QNVDD96 pKa = 3.43LSSS99 pKa = 3.09

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y7PPR0|A0A4Y7PPR0_9AGAM Uncharacterized protein OS=Rickenella mellea OX=50990 GN=BD410DRAFT_622211 PE=3 SV=1
MM1 pKa = 8.29RR2 pKa = 11.84GRR4 pKa = 11.84CLHH7 pKa = 5.93ARR9 pKa = 11.84NVRR12 pKa = 11.84GGRR15 pKa = 11.84SDD17 pKa = 3.48RR18 pKa = 11.84NMRR21 pKa = 11.84GTTLIVRR28 pKa = 11.84RR29 pKa = 11.84HH30 pKa = 6.01MIPNFGPSRR39 pKa = 11.84TRR41 pKa = 11.84TQPQSIPPKK50 pKa = 10.1HH51 pKa = 5.83FVALSTRR58 pKa = 11.84FLLGAFAILILVSHH72 pKa = 6.74LRR74 pKa = 11.84PRR76 pKa = 11.84LASLMVSTVHH86 pKa = 6.91PLRR89 pKa = 11.84PTSFVTSTKK98 pKa = 10.14SIHH101 pKa = 6.38PPRR104 pKa = 11.84PTRR107 pKa = 11.84ARR109 pKa = 11.84AA110 pKa = 3.04

Molecular weight: 12.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17053

0

17053

6715176

49

3910

393.8

43.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.126 ± 0.017

1.418 ± 0.01

5.585 ± 0.014

5.603 ± 0.018

3.885 ± 0.011

6.479 ± 0.018

2.716 ± 0.009

5.057 ± 0.014

4.49 ± 0.016

9.238 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.007

3.664 ± 0.011

6.339 ± 0.022

3.627 ± 0.012

6.305 ± 0.017

8.726 ± 0.023

6.221 ± 0.015

6.376 ± 0.013

1.48 ± 0.007

2.535 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski