Amino acid dipepetide frequency for Frigoribacterium sp. RIT-PI-h

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.252AlaAla: 19.252 ± 0.243
0.614AlaCys: 0.614 ± 0.031
8.613AlaAsp: 8.613 ± 0.113
7.298AlaGlu: 7.298 ± 0.108
3.812AlaPhe: 3.812 ± 0.073
12.286AlaGly: 12.286 ± 0.134
2.186AlaHis: 2.186 ± 0.059
4.575AlaIle: 4.575 ± 0.074
2.455AlaLys: 2.455 ± 0.072
13.578AlaLeu: 13.578 ± 0.167
2.466AlaMet: 2.466 ± 0.062
2.051AlaAsn: 2.051 ± 0.06
6.81AlaPro: 6.81 ± 0.132
3.323AlaGln: 3.323 ± 0.074
9.293AlaArg: 9.293 ± 0.135
8.225AlaSer: 8.225 ± 0.112
8.404AlaThr: 8.404 ± 0.127
12.377AlaVal: 12.377 ± 0.178
1.781AlaTrp: 1.781 ± 0.047
2.285AlaTyr: 2.285 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.522CysAla: 0.522 ± 0.025
0.047CysCys: 0.047 ± 0.009
0.299CysAsp: 0.299 ± 0.022
0.183CysGlu: 0.183 ± 0.015
0.156CysPhe: 0.156 ± 0.015
0.525CysGly: 0.525 ± 0.026
0.115CysHis: 0.115 ± 0.015
0.131CysIle: 0.131 ± 0.014
0.042CysLys: 0.042 ± 0.007
0.458CysLeu: 0.458 ± 0.024
0.059CysMet: 0.059 ± 0.009
0.083CysAsn: 0.083 ± 0.011
0.268CysPro: 0.268 ± 0.02
0.108CysGln: 0.108 ± 0.011
0.297CysArg: 0.297 ± 0.02
0.332CysSer: 0.332 ± 0.022
0.303CysThr: 0.303 ± 0.022
0.433CysVal: 0.433 ± 0.025
0.059CysTrp: 0.059 ± 0.009
0.076CysTyr: 0.076 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.827AspAla: 8.827 ± 0.121
0.24AspCys: 0.24 ± 0.017
5.654AspAsp: 5.654 ± 0.101
5.006AspGlu: 5.006 ± 0.09
1.733AspPhe: 1.733 ± 0.045
6.661AspGly: 6.661 ± 0.123
1.33AspHis: 1.33 ± 0.047
1.784AspIle: 1.784 ± 0.055
1.03AspLys: 1.03 ± 0.043
6.927AspLeu: 6.927 ± 0.099
0.768AspMet: 0.768 ± 0.031
0.863AspAsn: 0.863 ± 0.035
4.261AspPro: 4.261 ± 0.072
1.805AspGln: 1.805 ± 0.053
4.922AspArg: 4.922 ± 0.087
2.852AspSer: 2.852 ± 0.072
3.041AspThr: 3.041 ± 0.071
6.676AspVal: 6.676 ± 0.092
0.916AspTrp: 0.916 ± 0.033
1.229AspTyr: 1.229 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
7.25GluAla: 7.25 ± 0.101
0.194GluCys: 0.194 ± 0.016
2.037GluAsp: 2.037 ± 0.057
1.896GluGlu: 1.896 ± 0.054
1.641GluPhe: 1.641 ± 0.045
4.151GluGly: 4.151 ± 0.079
1.361GluHis: 1.361 ± 0.042
2.421GluIle: 2.421 ± 0.062
1.33GluLys: 1.33 ± 0.045
5.791GluLeu: 5.791 ± 0.093
0.816GluMet: 0.816 ± 0.031
1.097GluAsn: 1.097 ± 0.038
2.915GluPro: 2.915 ± 0.06
2.13GluGln: 2.13 ± 0.06
4.484GluArg: 4.484 ± 0.091
2.865GluSer: 2.865 ± 0.059
3.153GluThr: 3.153 ± 0.065
4.672GluVal: 4.672 ± 0.077
0.702GluTrp: 0.702 ± 0.028
1.033GluTyr: 1.033 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
4.023PheAla: 4.023 ± 0.077
0.15PheCys: 0.15 ± 0.013
2.427PheAsp: 2.427 ± 0.064
1.73PheGlu: 1.73 ± 0.046
1.081PhePhe: 1.081 ± 0.05
3.311PheGly: 3.311 ± 0.07
0.515PheHis: 0.515 ± 0.026
0.894PheIle: 0.894 ± 0.036
0.454PheLys: 0.454 ± 0.023
2.534PheLeu: 2.534 ± 0.067
0.435PheMet: 0.435 ± 0.024
0.546PheAsn: 0.546 ± 0.032
1.341PhePro: 1.341 ± 0.044
0.715PheGln: 0.715 ± 0.032
1.7PheArg: 1.7 ± 0.044
1.919PheSer: 1.919 ± 0.05
2.088PheThr: 2.088 ± 0.045
3.399PheVal: 3.399 ± 0.073
0.456PheTrp: 0.456 ± 0.027
0.598PheTyr: 0.598 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
11.058GlyAla: 11.058 ± 0.152
0.511GlyCys: 0.511 ± 0.024
5.797GlyAsp: 5.797 ± 0.082
4.438GlyGlu: 4.438 ± 0.081
3.241GlyPhe: 3.241 ± 0.067
8.461GlyGly: 8.461 ± 0.129
1.969GlyHis: 1.969 ± 0.054
3.971GlyIle: 3.971 ± 0.076
2.003GlyLys: 2.003 ± 0.052
9.55GlyLeu: 9.55 ± 0.121
1.643GlyMet: 1.643 ± 0.045
1.48GlyAsn: 1.48 ± 0.049
4.435GlyPro: 4.435 ± 0.075
2.723GlyGln: 2.723 ± 0.071
7.138GlyArg: 7.138 ± 0.119
6.045GlySer: 6.045 ± 0.095
6.351GlyThr: 6.351 ± 0.127
8.796GlyVal: 8.796 ± 0.109
1.532GlyTrp: 1.532 ± 0.046
2.069GlyTyr: 2.069 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.208HisAla: 2.208 ± 0.059
0.089HisCys: 0.089 ± 0.011
1.581HisAsp: 1.581 ± 0.044
1.234HisGlu: 1.234 ± 0.039
0.513HisPhe: 0.513 ± 0.021
1.982HisGly: 1.982 ± 0.046
0.486HisHis: 0.486 ± 0.025
0.496HisIle: 0.496 ± 0.025
0.245HisLys: 0.245 ± 0.015
1.984HisLeu: 1.984 ± 0.059
0.259HisMet: 0.259 ± 0.019
0.275HisAsn: 0.275 ± 0.019
1.399HisPro: 1.399 ± 0.045
0.49HisGln: 0.49 ± 0.024
1.582HisArg: 1.582 ± 0.05
0.863HisSer: 0.863 ± 0.03
0.902HisThr: 0.902 ± 0.038
1.89HisVal: 1.89 ± 0.055
0.262HisTrp: 0.262 ± 0.017
0.378HisTyr: 0.378 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
5.477IleAla: 5.477 ± 0.085
0.21IleCys: 0.21 ± 0.017
2.982IleAsp: 2.982 ± 0.07
2.223IleGlu: 2.223 ± 0.061
0.906IlePhe: 0.906 ± 0.038
4.074IleGly: 4.074 ± 0.077
0.527IleHis: 0.527 ± 0.023
1.221IleIle: 1.221 ± 0.047
0.75IleLys: 0.75 ± 0.03
2.554IleLeu: 2.554 ± 0.064
0.521IleMet: 0.521 ± 0.023
0.731IleAsn: 0.731 ± 0.033
1.734IlePro: 1.734 ± 0.05
0.768IleGln: 0.768 ± 0.031
2.219IleArg: 2.219 ± 0.054
2.026IleSer: 2.026 ± 0.049
2.532IleThr: 2.532 ± 0.061
4.389IleVal: 4.389 ± 0.077
0.4IleTrp: 0.4 ± 0.025
0.621IleTyr: 0.621 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
2.521LysAla: 2.521 ± 0.074
0.052LysCys: 0.052 ± 0.007
0.995LysAsp: 0.995 ± 0.033
0.854LysGlu: 0.854 ± 0.041
0.463LysPhe: 0.463 ± 0.023
1.664LysGly: 1.664 ± 0.048
0.432LysHis: 0.432 ± 0.024
0.869LysIle: 0.869 ± 0.039
0.875LysLys: 0.875 ± 0.045
1.748LysLeu: 1.748 ± 0.05
0.384LysMet: 0.384 ± 0.022
0.526LysAsn: 0.526 ± 0.03
1.125LysPro: 1.125 ± 0.042
0.699LysGln: 0.699 ± 0.03
1.421LysArg: 1.421 ± 0.043
1.148LysSer: 1.148 ± 0.039
1.353LysThr: 1.353 ± 0.041
1.7LysVal: 1.7 ± 0.056
0.208LysTrp: 0.208 ± 0.019
0.42LysTyr: 0.42 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
13.935LeuAla: 13.935 ± 0.163
0.393LeuCys: 0.393 ± 0.019
7.313LeuAsp: 7.313 ± 0.121
4.984LeuGlu: 4.984 ± 0.095
2.893LeuPhe: 2.893 ± 0.073
9.489LeuGly: 9.489 ± 0.118
1.728LeuHis: 1.728 ± 0.044
3.526LeuIle: 3.526 ± 0.082
1.756LeuLys: 1.756 ± 0.055
9.755LeuLeu: 9.755 ± 0.15
1.496LeuMet: 1.496 ± 0.041
1.543LeuAsn: 1.543 ± 0.046
5.601LeuPro: 5.601 ± 0.083
2.396LeuGln: 2.396 ± 0.055
6.997LeuArg: 6.997 ± 0.097
5.789LeuSer: 5.789 ± 0.097
6.357LeuThr: 6.357 ± 0.11
10.535LeuVal: 10.535 ± 0.15
1.209LeuTrp: 1.209 ± 0.041
1.576LeuTyr: 1.576 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
1.955MetAla: 1.955 ± 0.051
0.083MetCys: 0.083 ± 0.01
0.708MetAsp: 0.708 ± 0.028
0.519MetGlu: 0.519 ± 0.023
0.436MetPhe: 0.436 ± 0.022
1.182MetGly: 1.182 ± 0.039
0.311MetHis: 0.311 ± 0.018
0.761MetIle: 0.761 ± 0.032
0.397MetLys: 0.397 ± 0.018
1.647MetLeu: 1.647 ± 0.054
0.268MetMet: 0.268 ± 0.02
0.423MetAsn: 0.423 ± 0.022
1.035MetPro: 1.035 ± 0.037
0.53MetGln: 0.53 ± 0.028
1.247MetArg: 1.247 ± 0.036
1.353MetSer: 1.353 ± 0.039
1.703MetThr: 1.703 ± 0.039
1.344MetVal: 1.344 ± 0.044
0.148MetTrp: 0.148 ± 0.012
0.217MetTyr: 0.217 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.153AsnAla: 2.153 ± 0.053
0.093AsnCys: 0.093 ± 0.011
1.165AsnAsp: 1.165 ± 0.04
0.868AsnGlu: 0.868 ± 0.034
0.509AsnPhe: 0.509 ± 0.029
1.719AsnGly: 1.719 ± 0.052
0.341AsnHis: 0.341 ± 0.02
0.653AsnIle: 0.653 ± 0.032
0.416AsnLys: 0.416 ± 0.024
1.638AsnLeu: 1.638 ± 0.05
0.292AsnMet: 0.292 ± 0.021
0.4AsnAsn: 0.4 ± 0.023
1.344AsnPro: 1.344 ± 0.045
0.522AsnGln: 0.522 ± 0.027
1.125AsnArg: 1.125 ± 0.039
0.917AsnSer: 0.917 ± 0.032
0.996AsnThr: 0.996 ± 0.039
1.719AsnVal: 1.719 ± 0.05
0.256AsnTrp: 0.256 ± 0.018
0.437AsnTyr: 0.437 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
7.43ProAla: 7.43 ± 0.117
0.208ProCys: 0.208 ± 0.015
4.099ProAsp: 4.099 ± 0.074
3.421ProGlu: 3.421 ± 0.065
1.698ProPhe: 1.698 ± 0.043
5.519ProGly: 5.519 ± 0.103
1.034ProHis: 1.034 ± 0.038
1.708ProIle: 1.708 ± 0.045
0.967ProLys: 0.967 ± 0.034
4.876ProLeu: 4.876 ± 0.093
0.813ProMet: 0.813 ± 0.036
0.939ProAsn: 0.939 ± 0.034
2.721ProPro: 2.721 ± 0.084
1.402ProGln: 1.402 ± 0.046
3.682ProArg: 3.682 ± 0.071
3.508ProSer: 3.508 ± 0.077
4.138ProThr: 4.138 ± 0.079
5.611ProVal: 5.611 ± 0.093
0.828ProTrp: 0.828 ± 0.03
1.04ProTyr: 1.04 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
3.7GlnAla: 3.7 ± 0.078
0.096GlnCys: 0.096 ± 0.011
1.202GlnAsp: 1.202 ± 0.035
0.996GlnGlu: 0.996 ± 0.036
0.816GlnPhe: 0.816 ± 0.027
2.396GlnGly: 2.396 ± 0.06
0.635GlnHis: 0.635 ± 0.028
1.256GlnIle: 1.256 ± 0.039
0.699GlnLys: 0.699 ± 0.035
3.104GlnLeu: 3.104 ± 0.068
0.463GlnMet: 0.463 ± 0.024
0.639GlnAsn: 0.639 ± 0.03
1.575GlnPro: 1.575 ± 0.054
1.368GlnGln: 1.368 ± 0.059
2.128GlnArg: 2.128 ± 0.056
1.445GlnSer: 1.445 ± 0.049
1.567GlnThr: 1.567 ± 0.051
2.635GlnVal: 2.635 ± 0.059
0.363GlnTrp: 0.363 ± 0.019
0.61GlnTyr: 0.61 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
8.936ArgAla: 8.936 ± 0.128
0.316ArgCys: 0.316 ± 0.019
4.662ArgAsp: 4.662 ± 0.101
3.959ArgGlu: 3.959 ± 0.072
2.234ArgPhe: 2.234 ± 0.054
5.693ArgGly: 5.693 ± 0.094
1.696ArgHis: 1.696 ± 0.046
2.725ArgIle: 2.725 ± 0.058
1.256ArgLys: 1.256 ± 0.045
7.668ArgLeu: 7.668 ± 0.11
1.429ArgMet: 1.429 ± 0.046
1.098ArgAsn: 1.098 ± 0.037
4.237ArgPro: 4.237 ± 0.083
2.124ArgGln: 2.124 ± 0.058
7.066ArgArg: 7.066 ± 0.13
4.334ArgSer: 4.334 ± 0.073
4.147ArgThr: 4.147 ± 0.072
6.435ArgVal: 6.435 ± 0.097
1.113ArgTrp: 1.113 ± 0.038
1.408ArgTyr: 1.408 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
7.315SerAla: 7.315 ± 0.102
0.299SerCys: 0.299 ± 0.021
3.51SerAsp: 3.51 ± 0.076
2.476SerGlu: 2.476 ± 0.066
2.022SerPhe: 2.022 ± 0.059
6.15SerGly: 6.15 ± 0.081
1.044SerHis: 1.044 ± 0.038
2.484SerIle: 2.484 ± 0.06
1.222SerLys: 1.222 ± 0.04
5.562SerLeu: 5.562 ± 0.088
1.189SerMet: 1.189 ± 0.037
1.157SerAsn: 1.157 ± 0.041
3.374SerPro: 3.374 ± 0.065
1.476SerGln: 1.476 ± 0.048
4.16SerArg: 4.16 ± 0.072
4.257SerSer: 4.257 ± 0.093
4.466SerThr: 4.466 ± 0.091
5.309SerVal: 5.309 ± 0.087
0.933SerTrp: 0.933 ± 0.036
1.338SerTyr: 1.338 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
8.31ThrAla: 8.31 ± 0.139
0.26ThrCys: 0.26 ± 0.017
4.009ThrAsp: 4.009 ± 0.085
2.882ThrGlu: 2.882 ± 0.065
1.923ThrPhe: 1.923 ± 0.051
6.528ThrGly: 6.528 ± 0.102
1.015ThrHis: 1.015 ± 0.036
2.748ThrIle: 2.748 ± 0.063
1.26ThrLys: 1.26 ± 0.041
6.083ThrLeu: 6.083 ± 0.081
0.987ThrMet: 0.987 ± 0.036
1.266ThrAsn: 1.266 ± 0.041
4.275ThrPro: 4.275 ± 0.084
1.44ThrGln: 1.44 ± 0.06
4.199ThrArg: 4.199 ± 0.071
4.142ThrSer: 4.142 ± 0.08
4.844ThrThr: 4.844 ± 0.127
6.842ThrVal: 6.842 ± 0.155
1.001ThrTrp: 1.001 ± 0.039
1.215ThrTyr: 1.215 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
13.129ValAla: 13.129 ± 0.16
0.448ValCys: 0.448 ± 0.025
7.359ValAsp: 7.359 ± 0.111
5.471ValGlu: 5.471 ± 0.089
3.044ValPhe: 3.044 ± 0.071
8.501ValGly: 8.501 ± 0.139
1.819ValHis: 1.819 ± 0.049
3.635ValIle: 3.635 ± 0.071
1.674ValLys: 1.674 ± 0.054
10.153ValLeu: 10.153 ± 0.144
1.462ValMet: 1.462 ± 0.052
1.662ValAsn: 1.662 ± 0.048
5.506ValPro: 5.506 ± 0.092
2.409ValGln: 2.409 ± 0.05
6.273ValArg: 6.273 ± 0.101
5.532ValSer: 5.532 ± 0.085
6.956ValThr: 6.956 ± 0.147
12.013ValVal: 12.013 ± 0.188
1.129ValTrp: 1.129 ± 0.039
1.585ValTyr: 1.585 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
1.501TrpAla: 1.501 ± 0.044
0.096TrpCys: 0.096 ± 0.01
0.661TrpAsp: 0.661 ± 0.029
0.442TrpGlu: 0.442 ± 0.024
0.566TrpPhe: 0.566 ± 0.027
1.013TrpGly: 1.013 ± 0.036
0.316TrpHis: 0.316 ± 0.018
0.506TrpIle: 0.506 ± 0.028
0.271TrpLys: 0.271 ± 0.018
1.658TrpLeu: 1.658 ± 0.051
0.297TrpMet: 0.297 ± 0.02
0.373TrpAsn: 0.373 ± 0.023
0.718TrpPro: 0.718 ± 0.029
0.658TrpGln: 0.658 ± 0.024
1.127TrpArg: 1.127 ± 0.039
1.075TrpSer: 1.075 ± 0.036
0.841TrpThr: 0.841 ± 0.034
1.189TrpVal: 1.189 ± 0.038
0.329TrpTrp: 0.329 ± 0.022
0.255TrpTyr: 0.255 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.184TyrAla: 2.184 ± 0.051
0.1TyrCys: 0.1 ± 0.011
1.451TyrAsp: 1.451 ± 0.059
1.059TyrGlu: 1.059 ± 0.04
0.631TyrPhe: 0.631 ± 0.031
1.905TyrGly: 1.905 ± 0.062
0.258TyrHis: 0.258 ± 0.019
0.502TyrIle: 0.502 ± 0.029
0.357TyrLys: 0.357 ± 0.02
2.002TyrLeu: 2.002 ± 0.055
0.217TyrMet: 0.217 ± 0.017
0.411TyrAsn: 0.411 ± 0.025
0.969TyrPro: 0.969 ± 0.035
0.607TyrGln: 0.607 ± 0.028
1.475TyrArg: 1.475 ± 0.044
1.115TyrSer: 1.115 ± 0.038
1.088TyrThr: 1.088 ± 0.042
1.797TyrVal: 1.797 ± 0.045
0.265TyrTrp: 0.265 ± 0.019
0.397TyrTyr: 0.397 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2815 proteins (842116 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski