Amino acid dipepetide frequency for Limosilactobacillus secaliphilus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.952AlaAla: 7.952 ± 0.179
0.543AlaCys: 0.543 ± 0.035
5.489AlaAsp: 5.489 ± 0.107
3.816AlaGlu: 3.816 ± 0.096
3.206AlaPhe: 3.206 ± 0.097
6.313AlaGly: 6.313 ± 0.153
2.201AlaHis: 2.201 ± 0.071
5.426AlaIle: 5.426 ± 0.112
5.707AlaLys: 5.707 ± 0.135
7.334AlaLeu: 7.334 ± 0.152
2.307AlaMet: 2.307 ± 0.093
4.097AlaAsn: 4.097 ± 0.099
2.59AlaPro: 2.59 ± 0.092
3.93AlaGln: 3.93 ± 0.103
3.081AlaArg: 3.081 ± 0.093
4.604AlaSer: 4.604 ± 0.126
4.604AlaThr: 4.604 ± 0.147
5.793AlaVal: 5.793 ± 0.104
0.805AlaTrp: 0.805 ± 0.046
2.692AlaTyr: 2.692 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
0.493CysAla: 0.493 ± 0.034
0.037CysCys: 0.037 ± 0.009
0.358CysAsp: 0.358 ± 0.028
0.243CysGlu: 0.243 ± 0.025
0.308CysPhe: 0.308 ± 0.027
0.664CysGly: 0.664 ± 0.043
0.214CysHis: 0.214 ± 0.022
0.383CysIle: 0.383 ± 0.024
0.225CysLys: 0.225 ± 0.02
0.768CysLeu: 0.768 ± 0.049
0.133CysMet: 0.133 ± 0.014
0.193CysAsn: 0.193 ± 0.022
0.308CysPro: 0.308 ± 0.031
0.31CysGln: 0.31 ± 0.027
0.243CysArg: 0.243 ± 0.026
0.325CysSer: 0.325 ± 0.027
0.285CysThr: 0.285 ± 0.024
0.402CysVal: 0.402 ± 0.03
0.146CysTrp: 0.146 ± 0.017
0.241CysTyr: 0.241 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.363AspAla: 4.363 ± 0.122
0.36AspCys: 0.36 ± 0.03
4.342AspAsp: 4.342 ± 0.116
3.987AspGlu: 3.987 ± 0.118
2.726AspPhe: 2.726 ± 0.083
4.118AspGly: 4.118 ± 0.147
2.274AspHis: 2.274 ± 0.075
3.377AspIle: 3.377 ± 0.087
3.541AspLys: 3.541 ± 0.097
5.672AspLeu: 5.672 ± 0.109
1.592AspMet: 1.592 ± 0.066
2.886AspAsn: 2.886 ± 0.083
2.9AspPro: 2.9 ± 0.092
3.947AspGln: 3.947 ± 0.111
2.927AspArg: 2.927 ± 0.093
3.133AspSer: 3.133 ± 0.092
2.592AspThr: 2.592 ± 0.091
4.195AspVal: 4.195 ± 0.1
0.847AspTrp: 0.847 ± 0.05
2.763AspTyr: 2.763 ± 0.095
0.0AspXaa: 0.0 ± 0.0
Glu
3.733GluAla: 3.733 ± 0.102
0.221GluCys: 0.221 ± 0.02
2.807GluAsp: 2.807 ± 0.091
2.832GluGlu: 2.832 ± 0.107
1.769GluPhe: 1.769 ± 0.067
2.69GluGly: 2.69 ± 0.082
1.384GluHis: 1.384 ± 0.057
3.152GluIle: 3.152 ± 0.085
3.82GluLys: 3.82 ± 0.108
5.006GluLeu: 5.006 ± 0.115
1.671GluMet: 1.671 ± 0.064
2.372GluAsn: 2.372 ± 0.081
1.656GluPro: 1.656 ± 0.06
2.588GluGln: 2.588 ± 0.084
2.32GluArg: 2.32 ± 0.076
2.378GluSer: 2.378 ± 0.068
2.31GluThr: 2.31 ± 0.074
3.344GluVal: 3.344 ± 0.076
0.516GluTrp: 0.516 ± 0.038
1.696GluTyr: 1.696 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.446PheAla: 3.446 ± 0.084
0.366PheCys: 0.366 ± 0.028
2.942PheAsp: 2.942 ± 0.087
1.598PheGlu: 1.598 ± 0.06
1.816PhePhe: 1.816 ± 0.083
3.131PheGly: 3.131 ± 0.1
0.947PheHis: 0.947 ± 0.051
2.626PheIle: 2.626 ± 0.094
2.501PheLys: 2.501 ± 0.073
3.525PheLeu: 3.525 ± 0.129
1.124PheMet: 1.124 ± 0.05
2.23PheAsn: 2.23 ± 0.075
1.402PhePro: 1.402 ± 0.054
1.327PheGln: 1.327 ± 0.055
1.49PheArg: 1.49 ± 0.058
2.657PheSer: 2.657 ± 0.084
2.295PheThr: 2.295 ± 0.074
2.911PheVal: 2.911 ± 0.089
0.528PheTrp: 0.528 ± 0.039
1.579PheTyr: 1.579 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
4.821GlyAla: 4.821 ± 0.117
0.462GlyCys: 0.462 ± 0.033
3.907GlyAsp: 3.907 ± 0.097
3.123GlyGlu: 3.123 ± 0.074
3.023GlyPhe: 3.023 ± 0.093
4.305GlyGly: 4.305 ± 0.115
2.066GlyHis: 2.066 ± 0.07
4.835GlyIle: 4.835 ± 0.113
4.796GlyLys: 4.796 ± 0.116
6.294GlyLeu: 6.294 ± 0.124
2.128GlyMet: 2.128 ± 0.072
3.225GlyAsn: 3.225 ± 0.132
1.868GlyPro: 1.868 ± 0.067
3.61GlyGln: 3.61 ± 0.118
3.042GlyArg: 3.042 ± 0.094
3.943GlySer: 3.943 ± 0.155
4.022GlyThr: 4.022 ± 0.158
4.898GlyVal: 4.898 ± 0.107
1.001GlyTrp: 1.001 ± 0.061
2.826GlyTyr: 2.826 ± 0.077
0.0GlyXaa: 0.0 ± 0.0
His
1.952HisAla: 1.952 ± 0.081
0.208HisCys: 0.208 ± 0.019
1.814HisAsp: 1.814 ± 0.057
1.569HisGlu: 1.569 ± 0.06
1.398HisPhe: 1.398 ± 0.061
2.099HisGly: 2.099 ± 0.072
1.136HisHis: 1.136 ± 0.055
1.342HisIle: 1.342 ± 0.053
1.178HisLys: 1.178 ± 0.045
2.834HisLeu: 2.834 ± 0.089
0.624HisMet: 0.624 ± 0.032
1.236HisAsn: 1.236 ± 0.054
1.348HisPro: 1.348 ± 0.056
1.431HisGln: 1.431 ± 0.053
1.365HisArg: 1.365 ± 0.058
1.421HisSer: 1.421 ± 0.05
1.234HisThr: 1.234 ± 0.048
1.841HisVal: 1.841 ± 0.057
0.354HisTrp: 0.354 ± 0.031
1.517HisTyr: 1.517 ± 0.057
0.0HisXaa: 0.0 ± 0.0
Ile
5.36IleAla: 5.36 ± 0.119
0.572IleCys: 0.572 ± 0.036
4.321IleAsp: 4.321 ± 0.096
2.853IleGlu: 2.853 ± 0.086
2.642IlePhe: 2.642 ± 0.107
4.442IleGly: 4.442 ± 0.104
1.548IleHis: 1.548 ± 0.06
4.249IleIle: 4.249 ± 0.115
4.08IleLys: 4.08 ± 0.106
5.453IleLeu: 5.453 ± 0.143
1.735IleMet: 1.735 ± 0.066
3.425IleAsn: 3.425 ± 0.095
2.486IlePro: 2.486 ± 0.085
2.474IleGln: 2.474 ± 0.08
2.366IleArg: 2.366 ± 0.07
3.882IleSer: 3.882 ± 0.109
3.701IleThr: 3.701 ± 0.136
4.875IleVal: 4.875 ± 0.121
0.622IleTrp: 0.622 ± 0.037
2.191IleTyr: 2.191 ± 0.067
0.0IleXaa: 0.0 ± 0.0
Lys
4.771LysAla: 4.771 ± 0.119
0.218LysCys: 0.218 ± 0.022
3.943LysAsp: 3.943 ± 0.109
3.756LysGlu: 3.756 ± 0.096
1.766LysPhe: 1.766 ± 0.06
3.591LysGly: 3.591 ± 0.084
1.602LysHis: 1.602 ± 0.065
3.778LysIle: 3.778 ± 0.111
4.756LysLys: 4.756 ± 0.124
5.901LysLeu: 5.901 ± 0.113
2.428LysMet: 2.428 ± 0.072
3.077LysAsn: 3.077 ± 0.089
2.314LysPro: 2.314 ± 0.075
3.747LysGln: 3.747 ± 0.095
3.156LysArg: 3.156 ± 0.09
3.125LysSer: 3.125 ± 0.089
3.464LysThr: 3.464 ± 0.098
4.407LysVal: 4.407 ± 0.105
0.682LysTrp: 0.682 ± 0.04
2.303LysTyr: 2.303 ± 0.076
0.0LysXaa: 0.0 ± 0.0
Leu
8.526LeuAla: 8.526 ± 0.175
0.655LeuCys: 0.655 ± 0.032
5.58LeuAsp: 5.58 ± 0.124
3.527LeuGlu: 3.527 ± 0.108
3.699LeuPhe: 3.699 ± 0.115
6.369LeuGly: 6.369 ± 0.157
2.391LeuHis: 2.391 ± 0.077
6.352LeuIle: 6.352 ± 0.157
6.273LeuLys: 6.273 ± 0.116
9.057LeuLeu: 9.057 ± 0.233
2.798LeuMet: 2.798 ± 0.101
4.638LeuAsn: 4.638 ± 0.112
4.228LeuPro: 4.228 ± 0.098
4.299LeuGln: 4.299 ± 0.128
3.984LeuArg: 3.984 ± 0.116
6.219LeuSer: 6.219 ± 0.132
6.302LeuThr: 6.302 ± 0.139
6.8LeuVal: 6.8 ± 0.163
0.878LeuTrp: 0.878 ± 0.052
2.811LeuTyr: 2.811 ± 0.086
0.0LeuXaa: 0.0 ± 0.0
Met
2.647MetAla: 2.647 ± 0.082
0.156MetCys: 0.156 ± 0.019
1.683MetAsp: 1.683 ± 0.064
1.23MetGlu: 1.23 ± 0.054
0.924MetPhe: 0.924 ± 0.052
2.052MetGly: 2.052 ± 0.067
0.647MetHis: 0.647 ± 0.033
1.985MetIle: 1.985 ± 0.078
1.995MetLys: 1.995 ± 0.065
2.386MetLeu: 2.386 ± 0.079
0.972MetMet: 0.972 ± 0.053
1.456MetAsn: 1.456 ± 0.054
1.153MetPro: 1.153 ± 0.056
1.28MetGln: 1.28 ± 0.054
1.188MetArg: 1.188 ± 0.052
1.656MetSer: 1.656 ± 0.067
1.835MetThr: 1.835 ± 0.052
1.997MetVal: 1.997 ± 0.063
0.214MetTrp: 0.214 ± 0.021
0.707MetTyr: 0.707 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
3.756AsnAla: 3.756 ± 0.115
0.285AsnCys: 0.285 ± 0.027
3.202AsnAsp: 3.202 ± 0.087
2.505AsnGlu: 2.505 ± 0.077
1.893AsnPhe: 1.893 ± 0.062
4.009AsnGly: 4.009 ± 0.145
1.648AsnHis: 1.648 ± 0.065
2.551AsnIle: 2.551 ± 0.078
2.622AsnLys: 2.622 ± 0.117
4.066AsnLeu: 4.066 ± 0.101
1.203AsnMet: 1.203 ± 0.051
2.642AsnAsn: 2.642 ± 0.112
2.185AsnPro: 2.185 ± 0.073
2.632AsnGln: 2.632 ± 0.085
2.131AsnArg: 2.131 ± 0.066
2.744AsnSer: 2.744 ± 0.123
2.243AsnThr: 2.243 ± 0.073
3.271AsnVal: 3.271 ± 0.108
0.626AsnTrp: 0.626 ± 0.037
2.097AsnTyr: 2.097 ± 0.081
0.0AsnXaa: 0.0 ± 0.0
Pro
3.512ProAla: 3.512 ± 0.091
0.156ProCys: 0.156 ± 0.017
2.863ProAsp: 2.863 ± 0.094
2.524ProGlu: 2.524 ± 0.089
1.712ProPhe: 1.712 ± 0.065
2.43ProGly: 2.43 ± 0.071
0.957ProHis: 0.957 ± 0.05
2.307ProIle: 2.307 ± 0.075
2.172ProLys: 2.172 ± 0.073
3.547ProLeu: 3.547 ± 0.104
0.799ProMet: 0.799 ± 0.044
1.775ProAsn: 1.775 ± 0.059
0.566ProPro: 0.566 ± 0.039
1.933ProGln: 1.933 ± 0.073
1.388ProArg: 1.388 ± 0.064
2.305ProSer: 2.305 ± 0.09
2.79ProThr: 2.79 ± 0.416
3.111ProVal: 3.111 ± 0.08
0.42ProTrp: 0.42 ± 0.029
1.444ProTyr: 1.444 ± 0.057
0.0ProXaa: 0.0 ± 0.0
Gln
4.577GlnAla: 4.577 ± 0.12
0.229GlnCys: 0.229 ± 0.023
2.214GlnAsp: 2.214 ± 0.079
2.37GlnGlu: 2.37 ± 0.087
1.777GlnPhe: 1.777 ± 0.055
2.626GlnGly: 2.626 ± 0.071
1.319GlnHis: 1.319 ± 0.056
2.977GlnIle: 2.977 ± 0.085
3.073GlnLys: 3.073 ± 0.08
6.175GlnLeu: 6.175 ± 0.187
1.361GlnMet: 1.361 ± 0.053
1.975GlnAsn: 1.975 ± 0.07
2.099GlnPro: 2.099 ± 0.072
3.144GlnGln: 3.144 ± 0.127
2.609GlnArg: 2.609 ± 0.086
2.842GlnSer: 2.842 ± 0.088
2.778GlnThr: 2.778 ± 0.072
3.56GlnVal: 3.56 ± 0.097
0.566GlnTrp: 0.566 ± 0.036
1.727GlnTyr: 1.727 ± 0.064
0.0GlnXaa: 0.0 ± 0.0
Arg
3.034ArgAla: 3.034 ± 0.087
0.233ArgCys: 0.233 ± 0.025
2.291ArgAsp: 2.291 ± 0.078
2.26ArgGlu: 2.26 ± 0.078
2.11ArgPhe: 2.11 ± 0.073
2.536ArgGly: 2.536 ± 0.088
1.498ArgHis: 1.498 ± 0.059
2.726ArgIle: 2.726 ± 0.075
2.439ArgLys: 2.439 ± 0.076
4.765ArgLeu: 4.765 ± 0.138
1.271ArgMet: 1.271 ± 0.053
1.81ArgAsn: 1.81 ± 0.069
1.804ArgPro: 1.804 ± 0.063
2.672ArgGln: 2.672 ± 0.088
2.615ArgArg: 2.615 ± 0.088
2.081ArgSer: 2.081 ± 0.073
2.022ArgThr: 2.022 ± 0.069
2.998ArgVal: 2.998 ± 0.091
0.541ArgTrp: 0.541 ± 0.035
1.852ArgTyr: 1.852 ± 0.07
0.0ArgXaa: 0.0 ± 0.0
Ser
4.969SerAla: 4.969 ± 0.171
0.281SerCys: 0.281 ± 0.031
3.391SerAsp: 3.391 ± 0.104
2.422SerGlu: 2.422 ± 0.069
2.555SerPhe: 2.555 ± 0.073
4.415SerGly: 4.415 ± 0.163
1.486SerHis: 1.486 ± 0.065
3.377SerIle: 3.377 ± 0.094
3.246SerLys: 3.246 ± 0.094
5.892SerLeu: 5.892 ± 0.123
1.521SerMet: 1.521 ± 0.053
2.611SerAsn: 2.611 ± 0.119
2.143SerPro: 2.143 ± 0.231
3.009SerGln: 3.009 ± 0.078
2.642SerArg: 2.642 ± 0.085
3.793SerSer: 3.793 ± 0.148
3.142SerThr: 3.142 ± 0.11
3.787SerVal: 3.787 ± 0.086
0.762SerTrp: 0.762 ± 0.037
2.083SerTyr: 2.083 ± 0.082
0.0SerXaa: 0.0 ± 0.0
Thr
4.725ThrAla: 4.725 ± 0.172
0.331ThrCys: 0.331 ± 0.026
3.525ThrAsp: 3.525 ± 0.126
2.224ThrGlu: 2.224 ± 0.072
2.197ThrPhe: 2.197 ± 0.075
4.236ThrGly: 4.236 ± 0.141
1.232ThrHis: 1.232 ± 0.049
4.355ThrIle: 4.355 ± 0.166
3.421ThrLys: 3.421 ± 0.108
4.946ThrLeu: 4.946 ± 0.132
1.5ThrMet: 1.5 ± 0.065
2.738ThrAsn: 2.738 ± 0.107
2.83ThrPro: 2.83 ± 0.15
1.945ThrGln: 1.945 ± 0.063
1.941ThrArg: 1.941 ± 0.068
3.391ThrSer: 3.391 ± 0.255
3.537ThrThr: 3.537 ± 0.193
4.596ThrVal: 4.596 ± 0.133
0.622ThrTrp: 0.622 ± 0.047
1.975ThrTyr: 1.975 ± 0.154
0.0ThrXaa: 0.0 ± 0.0
Val
6.614ValAla: 6.614 ± 0.13
0.553ValCys: 0.553 ± 0.036
4.908ValAsp: 4.908 ± 0.116
3.233ValGlu: 3.233 ± 0.093
2.615ValPhe: 2.615 ± 0.082
4.875ValGly: 4.875 ± 0.119
1.627ValHis: 1.627 ± 0.056
4.912ValIle: 4.912 ± 0.121
4.875ValLys: 4.875 ± 0.107
6.267ValLeu: 6.267 ± 0.156
1.837ValMet: 1.837 ± 0.066
3.814ValAsn: 3.814 ± 0.105
2.915ValPro: 2.915 ± 0.077
2.68ValGln: 2.68 ± 0.085
2.574ValArg: 2.574 ± 0.082
4.232ValSer: 4.232 ± 0.088
4.488ValThr: 4.488 ± 0.132
5.661ValVal: 5.661 ± 0.128
0.705ValTrp: 0.705 ± 0.04
2.218ValTyr: 2.218 ± 0.071
0.002ValXaa: 0.002 ± 0.002
Trp
0.811TrpAla: 0.811 ± 0.044
0.108TrpCys: 0.108 ± 0.014
0.601TrpAsp: 0.601 ± 0.042
0.402TrpGlu: 0.402 ± 0.032
0.63TrpPhe: 0.63 ± 0.038
0.714TrpGly: 0.714 ± 0.039
0.487TrpHis: 0.487 ± 0.035
0.639TrpIle: 0.639 ± 0.044
0.495TrpLys: 0.495 ± 0.03
1.504TrpLeu: 1.504 ± 0.072
0.36TrpMet: 0.36 ± 0.027
0.506TrpAsn: 0.506 ± 0.033
0.389TrpPro: 0.389 ± 0.028
0.784TrpGln: 0.784 ± 0.042
0.545TrpArg: 0.545 ± 0.037
0.668TrpSer: 0.668 ± 0.041
0.637TrpThr: 0.637 ± 0.048
0.666TrpVal: 0.666 ± 0.039
0.235TrpTrp: 0.235 ± 0.022
0.433TrpTyr: 0.433 ± 0.034
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.432TyrAla: 2.432 ± 0.083
0.304TyrCys: 0.304 ± 0.027
2.359TyrAsp: 2.359 ± 0.078
1.791TyrGlu: 1.791 ± 0.064
1.673TyrPhe: 1.673 ± 0.053
2.58TyrGly: 2.58 ± 0.071
1.238TyrHis: 1.238 ± 0.056
1.929TyrIle: 1.929 ± 0.067
1.573TyrLys: 1.573 ± 0.071
3.997TyrLeu: 3.997 ± 0.096
0.797TyrMet: 0.797 ± 0.043
1.602TyrAsn: 1.602 ± 0.068
1.527TyrPro: 1.527 ± 0.06
2.255TyrGln: 2.255 ± 0.067
1.947TyrArg: 1.947 ± 0.069
2.124TyrSer: 2.124 ± 0.075
1.985TyrThr: 1.985 ± 0.168
2.497TyrVal: 2.497 ± 0.077
0.543TyrTrp: 0.543 ± 0.039
1.517TyrTyr: 1.517 ± 0.067
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.002XaaAsn: 0.002 ± 0.002
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.004XaaXaa: 0.004 ± 0.004
Statistics based on 1466 proteins (480622 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski