Lithobates catesbeianus (American bullfrog) (Rana catesbeiana)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Neobatrachia;

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G9QE81|A0A2G9QE81_LITCT Isoform of A0A2G9QEF6 Uncharacterized protein (Fragment) OS=Lithobates catesbeianus OX=8400 GN=AB205_0084550 PE=4 SV=1
MM1 pKa = 7.6CGAEE5 pKa = 4.07QRR7 pKa = 11.84DD8 pKa = 4.62DD9 pKa = 3.96MTSHH13 pKa = 7.05CPPHH17 pKa = 7.88ISALPPSQRR26 pKa = 11.84GPLQCRR32 pKa = 11.84RR33 pKa = 11.84CGGEE37 pKa = 3.66QRR39 pKa = 11.84DD40 pKa = 4.95CIISHH45 pKa = 6.99CPPCISALLSSLNGPVLPACHH66 pKa = 5.63QCSDD70 pKa = 3.7NAHH73 pKa = 6.09LVALAGMASDD83 pKa = 3.72NTHH86 pKa = 6.68LVASDD91 pKa = 3.61VASDD95 pKa = 3.77NLQMVASDD103 pKa = 3.7VASDD107 pKa = 3.78NLQMVAGDD115 pKa = 3.76MASDD119 pKa = 3.69NLQMVVGDD127 pKa = 3.81VASDD131 pKa = 3.59NLQMVASDD139 pKa = 3.64VTSDD143 pKa = 3.48NLQMVASDD151 pKa = 3.7VASDD155 pKa = 4.04NLQMMAGDD163 pKa = 3.85MASDD167 pKa = 3.92NPHH170 pKa = 5.52QVAGDD175 pKa = 3.72VASDD179 pKa = 3.92NPHH182 pKa = 5.47QVAGDD187 pKa = 3.61VARR190 pKa = 11.84DD191 pKa = 3.71NLQMVASDD199 pKa = 3.77VQSDD203 pKa = 3.88NLKK206 pKa = 9.65MVASDD211 pKa = 3.7NLQVMASDD219 pKa = 3.85VASDD223 pKa = 3.4NLQIVEE229 pKa = 4.39GDD231 pKa = 3.61VAICKK236 pKa = 8.31WWQQ239 pKa = 2.92

Molecular weight: 25.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G9QM72|A0A2G9QM72_LITCT SERPIN domain-containing protein OS=Lithobates catesbeianus OX=8400 GN=AB205_0132410 PE=3 SV=1
MM1 pKa = 7.27KK2 pKa = 10.52VSIRR6 pKa = 11.84GTRR9 pKa = 11.84RR10 pKa = 11.84IGVRR14 pKa = 11.84VTGRR18 pKa = 11.84VGVKK22 pKa = 9.76VIISGTRR29 pKa = 11.84RR30 pKa = 11.84VVIRR34 pKa = 11.84VTGKK38 pKa = 10.38ASVKK42 pKa = 9.86VSIRR46 pKa = 11.84GTRR49 pKa = 11.84RR50 pKa = 11.84AGVRR54 pKa = 11.84ITGRR58 pKa = 11.84VGVKK62 pKa = 9.54ISNRR66 pKa = 11.84GTRR69 pKa = 11.84RR70 pKa = 11.84VGFRR74 pKa = 11.84VAGRR78 pKa = 11.84VGVKK82 pKa = 10.14ARR84 pKa = 11.84IRR86 pKa = 11.84GTRR89 pKa = 11.84RR90 pKa = 11.84VGIRR94 pKa = 11.84VTGRR98 pKa = 11.84ASVKK102 pKa = 9.78VSIRR106 pKa = 11.84GTRR109 pKa = 11.84RR110 pKa = 11.84AGVRR114 pKa = 11.84VTGRR118 pKa = 11.84VSVKK122 pKa = 9.83VSNRR126 pKa = 11.84GAGRR130 pKa = 11.84VGVRR134 pKa = 11.84VTSRR138 pKa = 11.84VGVKK142 pKa = 9.55VSNRR146 pKa = 11.84GTRR149 pKa = 11.84RR150 pKa = 11.84VGFRR154 pKa = 11.84VAGRR158 pKa = 11.84VGVKK162 pKa = 9.58VRR164 pKa = 11.84IRR166 pKa = 11.84GPRR169 pKa = 11.84RR170 pKa = 11.84VGIRR174 pKa = 11.84VTGRR178 pKa = 11.84ASVKK182 pKa = 9.69VSNRR186 pKa = 11.84GTRR189 pKa = 11.84RR190 pKa = 11.84VGVRR194 pKa = 11.84VSDD197 pKa = 3.35GGTRR201 pKa = 11.84RR202 pKa = 11.84DD203 pKa = 3.45GFRR206 pKa = 11.84VAGKK210 pKa = 10.42VDD212 pKa = 3.44VKK214 pKa = 10.93LSIRR218 pKa = 11.84GTRR221 pKa = 11.84RR222 pKa = 11.84VGFRR226 pKa = 11.84VTGRR230 pKa = 3.29

Molecular weight: 24.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22112

6106

28218

7230904

24

9797

256.3

28.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.498 ± 0.016

2.471 ± 0.015

5.083 ± 0.015

7.21 ± 0.031

3.577 ± 0.014

5.973 ± 0.024

2.894 ± 0.012

5.161 ± 0.015

5.886 ± 0.021

8.805 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.01

4.056 ± 0.015

5.968 ± 0.025

4.727 ± 0.016

5.311 ± 0.017

8.834 ± 0.025

5.905 ± 0.025

6.179 ± 0.028

1.055 ± 0.006

2.943 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski