Blastochloris tepida

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Blastochloridaceae; Blastochloris

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3554 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A348FZ47|A0A348FZ47_9RHIZ Uncharacterized protein OS=Blastochloris tepida OX=2233851 GN=BLTE_12650 PE=4 SV=1
MM1 pKa = 7.61NIHH4 pKa = 7.28DD5 pKa = 3.98VTEE8 pKa = 5.01LIWHH12 pKa = 6.29FVGQLRR18 pKa = 11.84LDD20 pKa = 3.84EE21 pKa = 4.86DD22 pKa = 4.25LSRR25 pKa = 11.84SRR27 pKa = 11.84IQYY30 pKa = 9.73EE31 pKa = 3.76DD32 pKa = 3.8AEE34 pKa = 4.44AGRR37 pKa = 11.84VRR39 pKa = 11.84EE40 pKa = 4.15DD41 pKa = 2.99VFGRR45 pKa = 11.84VKK47 pKa = 10.51DD48 pKa = 3.59APVRR52 pKa = 11.84VEE54 pKa = 5.08DD55 pKa = 4.63PGEE58 pKa = 4.15SAPTPVSYY66 pKa = 9.13ATFAAAPALTPFVPHH81 pKa = 6.61FMQPGLASVPVEE93 pKa = 4.16PLDD96 pKa = 5.35DD97 pKa = 3.26IHH99 pKa = 7.01FKK101 pKa = 10.81VPPRR105 pKa = 11.84VLAAPAPGSLAGWYY119 pKa = 6.78PQSIGPEE126 pKa = 3.77YY127 pKa = 10.57HH128 pKa = 5.16QTLFEE133 pKa = 4.3ARR135 pKa = 11.84QINQLNDD142 pKa = 2.99SDD144 pKa = 4.61IFSDD148 pKa = 3.82DD149 pKa = 3.99PLWAVALPVAKK160 pKa = 7.74TTWSVDD166 pKa = 3.15EE167 pKa = 5.31LYY169 pKa = 9.91ATAQDD174 pKa = 5.07AIPAHH179 pKa = 6.78LEE181 pKa = 4.02TVAGGGSQEE190 pKa = 4.44AIAYY194 pKa = 6.66ATSLGSGAYY203 pKa = 9.41AFPSSQVEE211 pKa = 3.85PGSYY215 pKa = 10.52HH216 pKa = 5.4NGEE219 pKa = 4.6RR220 pKa = 11.84LAEE223 pKa = 4.39DD224 pKa = 3.08QTLPTDD230 pKa = 3.48IGATVPEE237 pKa = 4.77MPQPTNGPTGDD248 pKa = 4.55LGITTQAAEE257 pKa = 4.21LGANGAYY264 pKa = 10.19NSALIADD271 pKa = 4.27ANGACGTLVVLGDD284 pKa = 4.05KK285 pKa = 10.76FSLNAIIQINAYY297 pKa = 10.01SDD299 pKa = 3.5SDD301 pKa = 4.09VINAYY306 pKa = 10.06GGAGYY311 pKa = 8.08GTAGTIAGGGNQADD325 pKa = 4.0NLAQVGFTEE334 pKa = 4.77LEE336 pKa = 4.03AMPSAKK342 pKa = 10.2SGTPYY347 pKa = 10.55RR348 pKa = 11.84IDD350 pKa = 3.41VVEE353 pKa = 4.78GDD355 pKa = 3.75FFDD358 pKa = 5.22IRR360 pKa = 11.84TLYY363 pKa = 10.32QSNTLLDD370 pKa = 3.58SDD372 pKa = 4.43GASLTTEE379 pKa = 4.01GHH381 pKa = 6.06FSLVRR386 pKa = 11.84TGQNEE391 pKa = 4.41LVNLADD397 pKa = 5.64LSDD400 pKa = 4.44FDD402 pKa = 4.55LFSYY406 pKa = 10.95YY407 pKa = 10.75DD408 pKa = 3.51VIVIGGQYY416 pKa = 10.58YY417 pKa = 10.3DD418 pKa = 3.57INAIIQLNLLNDD430 pKa = 3.65NDD432 pKa = 3.97AVSAGGCGAGSGAGAGISTGGNSLLNDD459 pKa = 3.54ARR461 pKa = 11.84IEE463 pKa = 4.25NYY465 pKa = 8.03GTTNVQPLDD474 pKa = 3.57DD475 pKa = 5.26DD476 pKa = 3.81MAKK479 pKa = 10.61FSASLADD486 pKa = 4.18GDD488 pKa = 4.67APDD491 pKa = 5.01GASSWGFSDD500 pKa = 5.43GGDD503 pKa = 3.54GYY505 pKa = 10.75IDD507 pKa = 3.47VLYY510 pKa = 9.86VTGNFFDD517 pKa = 5.97INLLWQINTVTDD529 pKa = 3.0VDD531 pKa = 3.94AAALQAGTDD540 pKa = 3.78GSATQVVSTGANEE553 pKa = 4.28LQNQAIIVDD562 pKa = 3.95VDD564 pKa = 3.56ALTSEE569 pKa = 4.69YY570 pKa = 10.33IGGDD574 pKa = 3.43VYY576 pKa = 11.08EE577 pKa = 4.56GSILIQANLVEE588 pKa = 4.49SDD590 pKa = 3.39EE591 pKa = 4.98DD592 pKa = 4.17SVVHH596 pKa = 6.94HH597 pKa = 6.73DD598 pKa = 3.85TEE600 pKa = 4.57TLVPEE605 pKa = 4.8VIAFTGGSDD614 pKa = 4.05DD615 pKa = 5.32CGHH618 pKa = 7.11TDD620 pKa = 3.33PTYY623 pKa = 10.41VGPPVTMTDD632 pKa = 3.83HH633 pKa = 7.23ILGTVMTT640 pKa = 5.17

Molecular weight: 67.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A348G409|A0A348G409_9RHIZ TIGR02300 family protein OS=Blastochloris tepida OX=2233851 GN=BLTE_29770 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84KK29 pKa = 9.21VIAARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.21RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3554

0

3554

1106585

41

4128

311.4

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.196 ± 0.069

0.827 ± 0.014

5.678 ± 0.032

5.7 ± 0.046

3.494 ± 0.026

8.592 ± 0.046

1.921 ± 0.022

4.692 ± 0.032

2.946 ± 0.04

10.11 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.021

2.16 ± 0.025

5.712 ± 0.048

2.773 ± 0.022

7.921 ± 0.059

4.783 ± 0.035

5.287 ± 0.039

7.831 ± 0.033

1.264 ± 0.017

1.934 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski