Yongjia Tick Virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Uukuvirus; Yongjia uukuvirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KS85|A0A0B5KS85_9VIRU Nonstructural protein OS=Yongjia Tick Virus 1 OX=1608145 GN=NSs PE=4 SV=1
MM1 pKa = 7.21RR2 pKa = 11.84TEE4 pKa = 3.7TMMDD8 pKa = 3.01KK9 pKa = 11.24GFISQLDD16 pKa = 3.66PLLCSRR22 pKa = 11.84LGDD25 pKa = 3.74LLKK28 pKa = 10.38QLRR31 pKa = 11.84ATEE34 pKa = 4.07VGRR37 pKa = 11.84RR38 pKa = 11.84SAWATPTRR46 pKa = 11.84QTNNDD51 pKa = 3.52EE52 pKa = 5.01NEE54 pKa = 4.3VCPLPPMCTPLVYY67 pKa = 10.28PSVLTVQLKK76 pKa = 8.54VWPLEE81 pKa = 4.22GPLVSSPPHH90 pKa = 6.26LCLCVCVGFVCGG102 pKa = 4.55

Molecular weight: 11.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KS85|A0A0B5KS85_9VIRU Nonstructural protein OS=Yongjia Tick Virus 1 OX=1608145 GN=NSs PE=4 SV=1
MM1 pKa = 7.55AEE3 pKa = 4.64KK4 pKa = 10.19IRR6 pKa = 11.84FTGLPSLPDD15 pKa = 3.4VPTAADD21 pKa = 3.27WSRR24 pKa = 11.84VSFEE28 pKa = 4.07MGMTGAPEE36 pKa = 3.86VEE38 pKa = 5.08AITEE42 pKa = 3.93LSEE45 pKa = 3.86MFKK48 pKa = 10.64YY49 pKa = 10.36QGMDD53 pKa = 3.16PAVIIKK59 pKa = 10.29KK60 pKa = 9.67FATLGKK66 pKa = 8.92EE67 pKa = 4.05AHH69 pKa = 6.64RR70 pKa = 11.84NWLNDD75 pKa = 3.07AGFLIVLHH83 pKa = 6.69ICRR86 pKa = 11.84GTQVTKK92 pKa = 10.37IKK94 pKa = 9.81KK95 pKa = 7.59TVTPEE100 pKa = 3.41TATEE104 pKa = 4.02IEE106 pKa = 4.51TLVATYY112 pKa = 9.98KK113 pKa = 10.56IKK115 pKa = 10.59DD116 pKa = 3.75KK117 pKa = 11.3KK118 pKa = 10.53PVGDD122 pKa = 5.57DD123 pKa = 2.84ITLARR128 pKa = 11.84IALCFPLLTLRR139 pKa = 11.84QLSNFQDD146 pKa = 3.47HH147 pKa = 6.22LTVKK151 pKa = 8.76HH152 pKa = 5.7HH153 pKa = 6.64HH154 pKa = 5.89MEE156 pKa = 4.27EE157 pKa = 4.1LSAGYY162 pKa = 8.69PIQLMHH168 pKa = 6.76SAFASLITTTAGRR181 pKa = 11.84QQDD184 pKa = 3.96TQDD187 pKa = 3.95LLDD190 pKa = 3.37AHH192 pKa = 7.09RR193 pKa = 11.84LYY195 pKa = 10.92LVEE198 pKa = 4.0LTKK201 pKa = 10.9VINVGMKK208 pKa = 9.42GKK210 pKa = 7.82TVAEE214 pKa = 3.93IAEE217 pKa = 4.49SFEE220 pKa = 4.22QPLQAGFNSTFVSQSHH236 pKa = 6.13RR237 pKa = 11.84AASLRR242 pKa = 11.84KK243 pKa = 9.9LGVVDD248 pKa = 5.1GNGSPIAAVRR258 pKa = 11.84AAAQVYY264 pKa = 7.42RR265 pKa = 11.84TLKK268 pKa = 10.69ARR270 pKa = 11.84GG271 pKa = 3.36

Molecular weight: 29.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2511

102

2138

837.0

94.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.531 ± 1.415

1.513 ± 0.967

5.297 ± 0.501

7.527 ± 0.946

4.261 ± 0.489

5.297 ± 0.426

2.867 ± 0.337

5.536 ± 0.847

6.213 ± 0.594

9.478 ± 1.116

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.266 ± 0.133

3.067 ± 0.329

4.54 ± 1.164

3.266 ± 0.502

5.974 ± 0.447

8.244 ± 1.407

6.452 ± 1.042

6.252 ± 0.856

1.354 ± 0.21

3.067 ± 0.73
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski