Anaeromyxobacter dehalogenans (strain 2CP-C)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Cystobacterineae; Anaeromyxobacteraceae; Anaeromyxobacter; Anaeromyxobacter dehalogenans

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4345 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2IMM5|Q2IMM5_ANADE DNA-(apurinic or apyrimidinic site) lyase OS=Anaeromyxobacter dehalogenans (strain 2CP-C) OX=290397 GN=Adeh_0277 PE=3 SV=1
MM1 pKa = 7.14KK2 pKa = 9.79TLTKK6 pKa = 10.56LALTLIAVAGLQACDD21 pKa = 3.94PYY23 pKa = 11.24EE24 pKa = 4.48DD25 pKa = 4.14EE26 pKa = 4.65NKK28 pKa = 10.45GVPSVINVISSDD40 pKa = 3.09SGTAYY45 pKa = 10.28EE46 pKa = 4.47GTNTGTSWTISTPSAFVLFVKK67 pKa = 10.5VNAGKK72 pKa = 10.43LLDD75 pKa = 4.23GASIQATTDD84 pKa = 3.02DD85 pKa = 4.49CTPANDD91 pKa = 3.64WLSVNGAGPGNWMSCYY107 pKa = 10.06YY108 pKa = 8.81PQSPAGFEE116 pKa = 4.14GASVVLYY123 pKa = 8.04PGPSISTVDD132 pKa = 2.97SWFSLSEE139 pKa = 4.04LEE141 pKa = 4.49PGIFNLAGTVSDD153 pKa = 4.01KK154 pKa = 10.88QGHH157 pKa = 6.45PLAIDD162 pKa = 3.2VNAFVNDD169 pKa = 4.23LAAAQSDD176 pKa = 4.02TAGTEE181 pKa = 3.62ITTEE185 pKa = 3.97FSDD188 pKa = 4.6AGVNMTNVQLWEE200 pKa = 4.28APDD203 pKa = 3.46VSGAPGTWTAVATTTPYY220 pKa = 9.69VHH222 pKa = 7.05AGVAVGSTWWYY233 pKa = 10.83KK234 pKa = 8.59VTADD238 pKa = 3.88TNGFPATAASFEE250 pKa = 4.27TAPVSVTTEE259 pKa = 3.82PPAPP263 pKa = 3.85

Molecular weight: 27.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2IIL5|Q2IIL5_ANADE Molybdopterin binding domain protein OS=Anaeromyxobacter dehalogenans (strain 2CP-C) OX=290397 GN=Adeh_1720 PE=4 SV=1
MM1 pKa = 7.76APRR4 pKa = 11.84APLPRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84PRR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84SPGCPRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84GPRR28 pKa = 11.84LPRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84SRR36 pKa = 11.84LAGAGAARR44 pKa = 11.84RR45 pKa = 11.84GGPRR49 pKa = 11.84GGRR52 pKa = 11.84ARR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84ARR60 pKa = 11.84RR61 pKa = 11.84PDD63 pKa = 2.62GRR65 pKa = 11.84AARR68 pKa = 11.84RR69 pKa = 11.84QRR71 pKa = 11.84RR72 pKa = 11.84GAPGGRR78 pKa = 11.84RR79 pKa = 11.84GVRR82 pKa = 11.84GARR85 pKa = 11.84RR86 pKa = 11.84GRR88 pKa = 11.84GGRR91 pKa = 11.84GAGGAPGGSGRR102 pKa = 11.84DD103 pKa = 3.28GGGRR107 pKa = 11.84GRR109 pKa = 11.84ARR111 pKa = 11.84ARR113 pKa = 11.84RR114 pKa = 11.84HH115 pKa = 5.75AGPAAGARR123 pKa = 11.84RR124 pKa = 11.84AARR127 pKa = 11.84GLRR130 pKa = 11.84GRR132 pKa = 11.84LARR135 pKa = 11.84RR136 pKa = 11.84ARR138 pKa = 11.84RR139 pKa = 11.84HH140 pKa = 4.64RR141 pKa = 11.84RR142 pKa = 11.84RR143 pKa = 11.84ARR145 pKa = 11.84RR146 pKa = 11.84GGRR149 pKa = 11.84LAARR153 pKa = 11.84GGRR156 pKa = 11.84AGGARR161 pKa = 11.84ALGAQPGGPGRR172 pKa = 11.84PRR174 pKa = 11.84GGSRR178 pKa = 11.84GRR180 pKa = 11.84GGGDD184 pKa = 2.63RR185 pKa = 11.84RR186 pKa = 11.84EE187 pKa = 3.93RR188 pKa = 11.84PGRR191 pKa = 11.84GGGAARR197 pKa = 11.84AAARR201 pKa = 11.84PGAQPGPRR209 pKa = 11.84ARR211 pKa = 11.84RR212 pKa = 11.84GRR214 pKa = 11.84GARR217 pKa = 11.84AGRR220 pKa = 11.84GGGLGGAAGARR231 pKa = 11.84GAAALAGSGADD242 pKa = 2.83RR243 pKa = 11.84GRR245 pKa = 11.84RR246 pKa = 11.84GRR248 pKa = 11.84AGPSRR253 pKa = 11.84GSRR256 pKa = 11.84RR257 pKa = 11.84DD258 pKa = 2.96RR259 pKa = 11.84RR260 pKa = 11.84ARR262 pKa = 11.84GGGARR267 pKa = 11.84LVRR270 pKa = 11.84ADD272 pKa = 3.52RR273 pKa = 11.84GGGRR277 pKa = 11.84GRR279 pKa = 11.84ARR281 pKa = 11.84GAAGGARR288 pKa = 11.84GRR290 pKa = 11.84DD291 pKa = 3.22GALRR295 pKa = 11.84GGGAEE300 pKa = 3.76RR301 pKa = 11.84GSRR304 pKa = 11.84RR305 pKa = 11.84GGARR309 pKa = 11.84CARR312 pKa = 11.84GPAARR317 pKa = 11.84DD318 pKa = 3.46RR319 pKa = 11.84VPGAPVPGARR329 pKa = 11.84GPAGGRR335 pKa = 11.84GRR337 pKa = 11.84PGGARR342 pKa = 11.84GAGAHH347 pKa = 5.49GRR349 pKa = 11.84RR350 pKa = 11.84GGRR353 pKa = 11.84GRR355 pKa = 11.84GAPAPAPRR363 pKa = 11.84PRR365 pKa = 11.84PRR367 pKa = 11.84RR368 pKa = 11.84RR369 pKa = 11.84RR370 pKa = 11.84AARR373 pKa = 11.84RR374 pKa = 11.84RR375 pKa = 11.84RR376 pKa = 11.84ARR378 pKa = 11.84AGPARR383 pKa = 11.84RR384 pKa = 11.84PGLRR388 pKa = 11.84RR389 pKa = 11.84PGPRR393 pKa = 11.84PRR395 pKa = 11.84RR396 pKa = 11.84GAGRR400 pKa = 11.84APGGRR405 pKa = 11.84PGTAGRR411 pKa = 11.84VAAARR416 pKa = 11.84RR417 pKa = 11.84RR418 pKa = 11.84RR419 pKa = 11.84DD420 pKa = 2.99PCAARR425 pKa = 11.84RR426 pKa = 11.84DD427 pKa = 4.06APAHH431 pKa = 5.38RR432 pKa = 11.84RR433 pKa = 11.84RR434 pKa = 11.84RR435 pKa = 11.84RR436 pKa = 11.84ARR438 pKa = 11.84AGGAAGRR445 pKa = 11.84RR446 pKa = 11.84RR447 pKa = 11.84DD448 pKa = 3.39PARR451 pKa = 11.84AGRR454 pKa = 11.84GAAPARR460 pKa = 11.84GGARR464 pKa = 11.84CAPGGAHH471 pKa = 6.63RR472 pKa = 11.84RR473 pKa = 11.84DD474 pKa = 3.77RR475 pKa = 11.84RR476 pKa = 11.84PGGAGHH482 pKa = 7.12RR483 pKa = 11.84PGAAGRR489 pKa = 11.84GRR491 pKa = 11.84RR492 pKa = 11.84ARR494 pKa = 11.84GRR496 pKa = 11.84RR497 pKa = 11.84AGGAARR503 pKa = 11.84GRR505 pKa = 11.84RR506 pKa = 11.84GAGPGRR512 pKa = 11.84AALGAGAGARR522 pKa = 11.84GGAGGARR529 pKa = 11.84RR530 pKa = 11.84RR531 pKa = 11.84RR532 pKa = 11.84LALRR536 pKa = 11.84AGGGAGRR543 pKa = 11.84RR544 pKa = 11.84GRR546 pKa = 11.84GEE548 pKa = 3.87RR549 pKa = 11.84GGAAHH554 pKa = 7.44RR555 pKa = 11.84APRR558 pKa = 11.84RR559 pKa = 11.84RR560 pKa = 11.84VRR562 pKa = 11.84PGGGVRR568 pKa = 11.84ARR570 pKa = 11.84QDD572 pKa = 2.72RR573 pKa = 11.84RR574 pKa = 11.84AGRR577 pKa = 11.84GEE579 pKa = 3.88AAGGLAGAPGRR590 pKa = 11.84ARR592 pKa = 11.84GRR594 pKa = 11.84RR595 pKa = 11.84GDD597 pKa = 3.35RR598 pKa = 11.84GARR601 pKa = 11.84AARR604 pKa = 11.84RR605 pKa = 11.84PRR607 pKa = 11.84RR608 pKa = 11.84RR609 pKa = 11.84AGHH612 pKa = 6.02RR613 pKa = 11.84RR614 pKa = 11.84AAHH617 pKa = 5.68RR618 pKa = 11.84GSRR621 pKa = 11.84GGAGGGGAGHH631 pKa = 7.02RR632 pKa = 11.84PAPPRR637 pKa = 11.84GGRR640 pKa = 11.84AAAGGAAEE648 pKa = 4.12RR649 pKa = 11.84LPRR652 pKa = 11.84ARR654 pKa = 11.84AARR657 pKa = 11.84RR658 pKa = 11.84GGGAVAAPGGRR669 pKa = 11.84AADD672 pKa = 3.71AALKK676 pKa = 10.42AA677 pKa = 4.08

Molecular weight: 68.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4345

0

4345

1516487

38

4074

349.0

37.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.967 ± 0.084

0.884 ± 0.02

5.127 ± 0.024

6.159 ± 0.038

2.962 ± 0.023

9.348 ± 0.044

1.963 ± 0.017

2.933 ± 0.025

2.338 ± 0.036

10.854 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.635 ± 0.016

1.526 ± 0.019

6.364 ± 0.038

2.476 ± 0.019

9.386 ± 0.053

4.21 ± 0.024

4.387 ± 0.029

8.371 ± 0.034

1.284 ± 0.014

1.824 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski