Amino acid dipepetide frequency for Flavobacterium sp. TAB 87

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.956AlaAla: 4.956 ± 0.105
0.571AlaCys: 0.571 ± 0.024
3.64AlaAsp: 3.64 ± 0.074
4.094AlaGlu: 4.094 ± 0.082
3.432AlaPhe: 3.432 ± 0.057
4.146AlaGly: 4.146 ± 0.087
1.08AlaHis: 1.08 ± 0.033
5.906AlaIle: 5.906 ± 0.082
5.279AlaLys: 5.279 ± 0.103
6.501AlaLeu: 6.501 ± 0.085
1.622AlaMet: 1.622 ± 0.038
3.887AlaAsn: 3.887 ± 0.066
1.923AlaPro: 1.923 ± 0.056
2.603AlaGln: 2.603 ± 0.054
1.968AlaArg: 1.968 ± 0.045
4.278AlaSer: 4.278 ± 0.071
4.294AlaThr: 4.294 ± 0.094
4.682AlaVal: 4.682 ± 0.073
0.594AlaTrp: 0.594 ± 0.024
2.451AlaTyr: 2.451 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.469CysAla: 0.469 ± 0.024
0.109CysCys: 0.109 ± 0.011
0.421CysAsp: 0.421 ± 0.021
0.434CysGlu: 0.434 ± 0.022
0.431CysPhe: 0.431 ± 0.021
0.566CysGly: 0.566 ± 0.024
0.19CysHis: 0.19 ± 0.015
0.617CysIle: 0.617 ± 0.023
0.492CysLys: 0.492 ± 0.021
0.704CysLeu: 0.704 ± 0.025
0.162CysMet: 0.162 ± 0.012
0.418CysAsn: 0.418 ± 0.021
0.321CysPro: 0.321 ± 0.017
0.244CysGln: 0.244 ± 0.016
0.226CysArg: 0.226 ± 0.014
0.566CysSer: 0.566 ± 0.027
0.477CysThr: 0.477 ± 0.025
0.443CysVal: 0.443 ± 0.022
0.056CysTrp: 0.056 ± 0.007
0.306CysTyr: 0.306 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.861AspAla: 3.861 ± 0.08
0.433AspCys: 0.433 ± 0.022
2.563AspAsp: 2.563 ± 0.054
3.453AspGlu: 3.453 ± 0.068
3.521AspPhe: 3.521 ± 0.06
3.377AspGly: 3.377 ± 0.077
0.839AspHis: 0.839 ± 0.03
3.929AspIle: 3.929 ± 0.06
4.154AspLys: 4.154 ± 0.069
5.377AspLeu: 5.377 ± 0.072
1.058AspMet: 1.058 ± 0.033
2.949AspAsn: 2.949 ± 0.052
1.576AspPro: 1.576 ± 0.041
1.684AspGln: 1.684 ± 0.041
1.858AspArg: 1.858 ± 0.043
3.144AspSer: 3.144 ± 0.062
2.614AspThr: 2.614 ± 0.052
3.416AspVal: 3.416 ± 0.059
0.704AspTrp: 0.704 ± 0.024
2.641AspTyr: 2.641 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
4.26GluAla: 4.26 ± 0.082
0.345GluCys: 0.345 ± 0.018
3.054GluAsp: 3.054 ± 0.059
4.349GluGlu: 4.349 ± 0.083
2.9GluPhe: 2.9 ± 0.05
3.396GluGly: 3.396 ± 0.057
1.046GluHis: 1.046 ± 0.028
5.591GluIle: 5.591 ± 0.087
6.059GluLys: 6.059 ± 0.097
6.159GluLeu: 6.159 ± 0.093
1.644GluMet: 1.644 ± 0.043
4.712GluAsn: 4.712 ± 0.08
1.435GluPro: 1.435 ± 0.038
2.403GluGln: 2.403 ± 0.049
2.334GluArg: 2.334 ± 0.049
3.575GluSer: 3.575 ± 0.062
3.595GluThr: 3.595 ± 0.066
4.115GluVal: 4.115 ± 0.06
0.599GluTrp: 0.599 ± 0.026
2.345GluTyr: 2.345 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.338PheAla: 3.338 ± 0.06
0.486PheCys: 0.486 ± 0.022
3.396PheAsp: 3.396 ± 0.061
3.516PheGlu: 3.516 ± 0.052
2.794PhePhe: 2.794 ± 0.059
3.529PheGly: 3.529 ± 0.057
0.806PheHis: 0.806 ± 0.025
3.988PheIle: 3.988 ± 0.071
3.725PheLys: 3.725 ± 0.061
4.846PheLeu: 4.846 ± 0.067
1.161PheMet: 1.161 ± 0.033
3.115PheAsn: 3.115 ± 0.06
1.761PhePro: 1.761 ± 0.043
1.645PheGln: 1.645 ± 0.046
1.614PheArg: 1.614 ± 0.036
4.056PheSer: 4.056 ± 0.068
3.1PheThr: 3.1 ± 0.066
3.251PheVal: 3.251 ± 0.058
0.541PheTrp: 0.541 ± 0.026
2.257PheTyr: 2.257 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
4.134GlyAla: 4.134 ± 0.078
0.58GlyCys: 0.58 ± 0.033
2.959GlyAsp: 2.959 ± 0.052
3.17GlyGlu: 3.17 ± 0.052
3.523GlyPhe: 3.523 ± 0.066
4.211GlyGly: 4.211 ± 0.076
1.037GlyHis: 1.037 ± 0.036
5.392GlyIle: 5.392 ± 0.076
4.937GlyLys: 4.937 ± 0.072
5.61GlyLeu: 5.61 ± 0.088
1.577GlyMet: 1.577 ± 0.039
3.44GlyAsn: 3.44 ± 0.076
1.198GlyPro: 1.198 ± 0.035
1.866GlyGln: 1.866 ± 0.043
1.941GlyArg: 1.941 ± 0.042
4.008GlySer: 4.008 ± 0.068
3.941GlyThr: 3.941 ± 0.09
4.173GlyVal: 4.173 ± 0.076
0.691GlyTrp: 0.691 ± 0.03
2.727GlyTyr: 2.727 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
0.984HisAla: 0.984 ± 0.03
0.179HisCys: 0.179 ± 0.013
0.845HisAsp: 0.845 ± 0.03
0.969HisGlu: 0.969 ± 0.031
1.182HisPhe: 1.182 ± 0.034
0.962HisGly: 0.962 ± 0.034
0.452HisHis: 0.452 ± 0.021
1.299HisIle: 1.299 ± 0.035
1.142HisLys: 1.142 ± 0.036
1.759HisLeu: 1.759 ± 0.043
0.286HisMet: 0.286 ± 0.017
0.921HisAsn: 0.921 ± 0.031
0.787HisPro: 0.787 ± 0.025
0.753HisGln: 0.753 ± 0.026
0.573HisArg: 0.573 ± 0.023
1.028HisSer: 1.028 ± 0.028
0.925HisThr: 0.925 ± 0.026
0.819HisVal: 0.819 ± 0.026
0.191HisTrp: 0.191 ± 0.013
0.794HisTyr: 0.794 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
6.241IleAla: 6.241 ± 0.08
0.653IleCys: 0.653 ± 0.024
4.766IleAsp: 4.766 ± 0.072
5.501IleGlu: 5.501 ± 0.08
3.693IlePhe: 3.693 ± 0.062
5.024IleGly: 5.024 ± 0.067
1.317IleHis: 1.317 ± 0.043
6.421IleIle: 6.421 ± 0.095
6.14IleLys: 6.14 ± 0.082
7.186IleLeu: 7.186 ± 0.1
1.46IleMet: 1.46 ± 0.037
4.618IleAsn: 4.618 ± 0.077
3.243IlePro: 3.243 ± 0.056
2.838IleGln: 2.838 ± 0.054
2.635IleArg: 2.635 ± 0.05
5.628IleSer: 5.628 ± 0.083
4.85IleThr: 4.85 ± 0.083
5.156IleVal: 5.156 ± 0.086
0.696IleTrp: 0.696 ± 0.027
2.769IleTyr: 2.769 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
5.151LysAla: 5.151 ± 0.096
0.412LysCys: 0.412 ± 0.02
4.145LysAsp: 4.145 ± 0.066
6.087LysGlu: 6.087 ± 0.101
3.006LysPhe: 3.006 ± 0.055
4.496LysGly: 4.496 ± 0.07
1.228LysHis: 1.228 ± 0.039
6.978LysIle: 6.978 ± 0.091
7.033LysLys: 7.033 ± 0.116
6.658LysLeu: 6.658 ± 0.082
2.263LysMet: 2.263 ± 0.052
5.695LysAsn: 5.695 ± 0.082
2.434LysPro: 2.434 ± 0.041
2.923LysGln: 2.923 ± 0.059
2.669LysArg: 2.669 ± 0.059
4.917LysSer: 4.917 ± 0.065
4.721LysThr: 4.721 ± 0.068
4.895LysVal: 4.895 ± 0.075
0.783LysTrp: 0.783 ± 0.031
3.104LysTyr: 3.104 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
6.202LeuAla: 6.202 ± 0.097
0.713LeuCys: 0.713 ± 0.03
4.795LeuAsp: 4.795 ± 0.069
5.973LeuGlu: 5.973 ± 0.088
5.147LeuPhe: 5.147 ± 0.087
5.7LeuGly: 5.7 ± 0.077
1.666LeuHis: 1.666 ± 0.039
6.988LeuIle: 6.988 ± 0.096
7.615LeuLys: 7.615 ± 0.09
9.182LeuLeu: 9.182 ± 0.121
2.187LeuMet: 2.187 ± 0.05
5.685LeuAsn: 5.685 ± 0.09
3.495LeuPro: 3.495 ± 0.056
3.746LeuGln: 3.746 ± 0.063
3.093LeuArg: 3.093 ± 0.059
6.745LeuSer: 6.745 ± 0.078
5.07LeuThr: 5.07 ± 0.068
5.441LeuVal: 5.441 ± 0.079
0.794LeuTrp: 0.794 ± 0.025
3.327LeuTyr: 3.327 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
1.639MetAla: 1.639 ± 0.04
0.156MetCys: 0.156 ± 0.011
1.177MetAsp: 1.177 ± 0.036
1.352MetGlu: 1.352 ± 0.04
0.839MetPhe: 0.839 ± 0.034
1.362MetGly: 1.362 ± 0.033
0.411MetHis: 0.411 ± 0.02
1.716MetIle: 1.716 ± 0.048
2.191MetLys: 2.191 ± 0.047
1.9MetLeu: 1.9 ± 0.043
0.606MetMet: 0.606 ± 0.027
1.431MetAsn: 1.431 ± 0.038
0.85MetPro: 0.85 ± 0.029
0.98MetGln: 0.98 ± 0.031
0.847MetArg: 0.847 ± 0.027
1.484MetSer: 1.484 ± 0.035
1.188MetThr: 1.188 ± 0.029
1.34MetVal: 1.34 ± 0.039
0.188MetTrp: 0.188 ± 0.016
0.746MetTyr: 0.746 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
4.022AsnAla: 4.022 ± 0.067
0.504AsnCys: 0.504 ± 0.025
3.17AsnAsp: 3.17 ± 0.054
3.701AsnGlu: 3.701 ± 0.065
3.291AsnPhe: 3.291 ± 0.057
3.796AsnGly: 3.796 ± 0.08
1.087AsnHis: 1.087 ± 0.035
4.545AsnIle: 4.545 ± 0.07
4.633AsnLys: 4.633 ± 0.067
5.502AsnLeu: 5.502 ± 0.076
1.247AsnMet: 1.247 ± 0.033
3.64AsnAsn: 3.64 ± 0.071
2.62AsnPro: 2.62 ± 0.05
2.435AsnGln: 2.435 ± 0.053
2.104AsnArg: 2.104 ± 0.046
4.157AsnSer: 4.157 ± 0.07
3.521AsnThr: 3.521 ± 0.077
3.546AsnVal: 3.546 ± 0.069
0.808AsnTrp: 0.808 ± 0.031
2.922AsnTyr: 2.922 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
2.041ProAla: 2.041 ± 0.052
0.18ProCys: 0.18 ± 0.013
1.782ProAsp: 1.782 ± 0.036
2.626ProGlu: 2.626 ± 0.053
1.904ProPhe: 1.904 ± 0.044
1.705ProGly: 1.705 ± 0.042
0.559ProHis: 0.559 ± 0.022
2.743ProIle: 2.743 ± 0.057
2.523ProLys: 2.523 ± 0.053
2.914ProLeu: 2.914 ± 0.055
0.709ProMet: 0.709 ± 0.025
2.142ProAsn: 2.142 ± 0.044
0.705ProPro: 0.705 ± 0.028
1.103ProGln: 1.103 ± 0.032
0.885ProArg: 0.885 ± 0.032
2.044ProSer: 2.044 ± 0.048
2.134ProThr: 2.134 ± 0.056
2.199ProVal: 2.199 ± 0.051
0.304ProTrp: 0.304 ± 0.018
1.338ProTyr: 1.338 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
2.163GlnAla: 2.163 ± 0.05
0.183GlnCys: 0.183 ± 0.013
1.645GlnAsp: 1.645 ± 0.035
2.446GlnGlu: 2.446 ± 0.049
1.875GlnPhe: 1.875 ± 0.041
1.795GlnGly: 1.795 ± 0.042
0.594GlnHis: 0.594 ± 0.024
3.022GlnIle: 3.022 ± 0.049
3.332GlnLys: 3.332 ± 0.06
3.732GlnLeu: 3.732 ± 0.063
0.87GlnMet: 0.87 ± 0.03
2.492GlnAsn: 2.492 ± 0.056
1.066GlnPro: 1.066 ± 0.032
1.5GlnGln: 1.5 ± 0.042
1.269GlnArg: 1.269 ± 0.036
2.285GlnSer: 2.285 ± 0.048
2.073GlnThr: 2.073 ± 0.038
1.966GlnVal: 1.966 ± 0.043
0.368GlnTrp: 0.368 ± 0.019
1.4GlnTyr: 1.4 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
2.118ArgAla: 2.118 ± 0.049
0.192ArgCys: 0.192 ± 0.013
1.657ArgAsp: 1.657 ± 0.04
2.138ArgGlu: 2.138 ± 0.046
1.849ArgPhe: 1.849 ± 0.037
1.76ArgGly: 1.76 ± 0.041
0.585ArgHis: 0.585 ± 0.026
2.823ArgIle: 2.823 ± 0.056
2.715ArgLys: 2.715 ± 0.055
3.126ArgLeu: 3.126 ± 0.058
0.915ArgMet: 0.915 ± 0.028
2.035ArgAsn: 2.035 ± 0.05
1.014ArgPro: 1.014 ± 0.034
1.008ArgGln: 1.008 ± 0.03
1.157ArgArg: 1.157 ± 0.041
1.996ArgSer: 1.996 ± 0.049
1.849ArgThr: 1.849 ± 0.048
1.989ArgVal: 1.989 ± 0.044
0.347ArgTrp: 0.347 ± 0.019
1.414ArgTyr: 1.414 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
4.157SerAla: 4.157 ± 0.073
0.649SerCys: 0.649 ± 0.029
3.522SerAsp: 3.522 ± 0.062
3.965SerGlu: 3.965 ± 0.074
4.005SerPhe: 4.005 ± 0.069
4.433SerGly: 4.433 ± 0.074
1.115SerHis: 1.115 ± 0.033
5.248SerIle: 5.248 ± 0.076
5.224SerLys: 5.224 ± 0.072
6.018SerLeu: 6.018 ± 0.085
1.305SerMet: 1.305 ± 0.033
3.95SerAsn: 3.95 ± 0.074
2.014SerPro: 2.014 ± 0.045
2.218SerGln: 2.218 ± 0.047
2.114SerArg: 2.114 ± 0.046
4.495SerSer: 4.495 ± 0.089
3.874SerThr: 3.874 ± 0.066
4.118SerVal: 4.118 ± 0.058
0.694SerTrp: 0.694 ± 0.024
2.848SerTyr: 2.848 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
4.377ThrAla: 4.377 ± 0.083
0.339ThrCys: 0.339 ± 0.019
3.344ThrAsp: 3.344 ± 0.063
3.509ThrGlu: 3.509 ± 0.062
3.076ThrPhe: 3.076 ± 0.06
3.803ThrGly: 3.803 ± 0.086
0.914ThrHis: 0.914 ± 0.035
5.321ThrIle: 5.321 ± 0.096
4.092ThrLys: 4.092 ± 0.058
5.252ThrLeu: 5.252 ± 0.072
1.024ThrMet: 1.024 ± 0.03
3.427ThrAsn: 3.427 ± 0.068
2.293ThrPro: 2.293 ± 0.044
2.03ThrGln: 2.03 ± 0.042
1.557ThrArg: 1.557 ± 0.038
3.704ThrSer: 3.704 ± 0.063
3.835ThrThr: 3.835 ± 0.089
4.082ThrVal: 4.082 ± 0.09
0.502ThrTrp: 0.502 ± 0.022
2.216ThrTyr: 2.216 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
4.707ValAla: 4.707 ± 0.069
0.525ValCys: 0.525 ± 0.023
3.426ValAsp: 3.426 ± 0.059
3.686ValGlu: 3.686 ± 0.059
3.399ValPhe: 3.399 ± 0.058
3.873ValGly: 3.873 ± 0.069
0.992ValHis: 0.992 ± 0.034
4.853ValIle: 4.853 ± 0.075
4.447ValLys: 4.447 ± 0.07
6.387ValLeu: 6.387 ± 0.083
1.315ValMet: 1.315 ± 0.038
3.464ValAsn: 3.464 ± 0.06
2.202ValPro: 2.202 ± 0.048
2.043ValGln: 2.043 ± 0.048
2.021ValArg: 2.021 ± 0.049
4.392ValSer: 4.392 ± 0.067
3.818ValThr: 3.818 ± 0.082
4.405ValVal: 4.405 ± 0.072
0.542ValTrp: 0.542 ± 0.023
2.286ValTyr: 2.286 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.6TrpAla: 0.6 ± 0.021
0.095TrpCys: 0.095 ± 0.009
0.537TrpAsp: 0.537 ± 0.023
0.661TrpGlu: 0.661 ± 0.025
0.527TrpPhe: 0.527 ± 0.023
0.583TrpGly: 0.583 ± 0.026
0.207TrpHis: 0.207 ± 0.012
0.794TrpIle: 0.794 ± 0.031
0.82TrpLys: 0.82 ± 0.028
0.963TrpLeu: 0.963 ± 0.032
0.27TrpMet: 0.27 ± 0.015
0.695TrpAsn: 0.695 ± 0.028
0.194TrpPro: 0.194 ± 0.014
0.432TrpGln: 0.432 ± 0.019
0.4TrpArg: 0.4 ± 0.019
0.62TrpSer: 0.62 ± 0.028
0.534TrpThr: 0.534 ± 0.023
0.522TrpVal: 0.522 ± 0.024
0.131TrpTrp: 0.131 ± 0.011
0.4TrpTyr: 0.4 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.486TyrAla: 2.486 ± 0.054
0.349TyrCys: 0.349 ± 0.014
2.308TyrAsp: 2.308 ± 0.047
2.285TyrGlu: 2.285 ± 0.054
2.531TyrPhe: 2.531 ± 0.045
2.596TyrGly: 2.596 ± 0.057
0.724TyrHis: 0.724 ± 0.031
2.661TyrIle: 2.661 ± 0.05
3.044TyrLys: 3.044 ± 0.057
3.789TyrLeu: 3.789 ± 0.063
0.723TyrMet: 0.723 ± 0.027
2.536TyrAsn: 2.536 ± 0.053
1.408TyrPro: 1.408 ± 0.04
1.624TyrGln: 1.624 ± 0.043
1.423TyrArg: 1.423 ± 0.035
2.842TyrSer: 2.842 ± 0.059
2.284TyrThr: 2.284 ± 0.063
2.224TyrVal: 2.224 ± 0.042
0.455TyrTrp: 0.455 ± 0.018
1.807TyrTyr: 1.807 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3323 proteins (1112781 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski