Amino acid dipepetide frequency for Bacillus caseinilyticus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.974AlaAla: 7.974 ± 0.101	0.728AlaCys: 0.728 ± 0.025	4.176AlaAsp: 4.176 ± 0.055	5.774AlaGlu: 5.774 ± 0.069	3.614AlaPhe: 3.614 ± 0.056	6.251AlaGly: 6.251 ± 0.07	1.276AlaHis: 1.276 ± 0.03	5.354AlaIle: 5.354 ± 0.06	4.327AlaLys: 4.327 ± 0.061	7.554AlaLeu: 7.554 ± 0.078	2.191AlaMet: 2.191 ± 0.043	2.761AlaAsn: 2.761 ± 0.047	2.283AlaPro: 2.283 ± 0.044	2.167AlaGln: 2.167 ± 0.04	2.865AlaArg: 2.865 ± 0.044	4.138AlaSer: 4.138 ± 0.053	3.5AlaThr: 3.5 ± 0.048	6.812AlaVal: 6.812 ± 0.074	0.734AlaTrp: 0.734 ± 0.025	2.497AlaTyr: 2.497 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.495CysAla: 0.495 ± 0.02	0.122CysCys: 0.122 ± 0.009	0.402CysAsp: 0.402 ± 0.017	0.462CysGlu: 0.462 ± 0.017	0.408CysPhe: 0.408 ± 0.016	0.704CysGly: 0.704 ± 0.025	0.245CysHis: 0.245 ± 0.013	0.551CysIle: 0.551 ± 0.02	0.328CysLys: 0.328 ± 0.015	0.768CysLeu: 0.768 ± 0.024	0.2CysMet: 0.2 ± 0.011	0.279CysAsn: 0.279 ± 0.013	0.396CysPro: 0.396 ± 0.018	0.272CysGln: 0.272 ± 0.014	0.436CysArg: 0.436 ± 0.017	0.587CysSer: 0.587 ± 0.021	0.396CysThr: 0.396 ± 0.017	0.438CysVal: 0.438 ± 0.017	0.094CysTrp: 0.094 ± 0.008	0.276CysTyr: 0.276 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.447AspAla: 3.447 ± 0.049	0.387AspCys: 0.387 ± 0.016	2.849AspAsp: 2.849 ± 0.054	4.299AspGlu: 4.299 ± 0.052	2.312AspPhe: 2.312 ± 0.034	3.919AspGly: 3.919 ± 0.06	1.197AspHis: 1.197 ± 0.029	4.036AspIle: 4.036 ± 0.051	2.765AspLys: 2.765 ± 0.043	4.392AspLeu: 4.392 ± 0.059	1.324AspMet: 1.324 ± 0.029	1.987AspAsn: 1.987 ± 0.041	2.069AspPro: 2.069 ± 0.037	1.823AspGln: 1.823 ± 0.04	2.503AspArg: 2.503 ± 0.048	2.604AspSer: 2.604 ± 0.045	2.47AspThr: 2.47 ± 0.041	3.806AspVal: 3.806 ± 0.051	0.735AspTrp: 0.735 ± 0.024	2.197AspTyr: 2.197 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	6.271GluAla: 6.271 ± 0.076	0.386GluCys: 0.386 ± 0.017	3.56GluAsp: 3.56 ± 0.065	7.225GluGlu: 7.225 ± 0.098	2.272GluPhe: 2.272 ± 0.039	4.128GluGly: 4.128 ± 0.058	1.544GluHis: 1.544 ± 0.033	5.088GluIle: 5.088 ± 0.064	6.291GluLys: 6.291 ± 0.073	7.16GluLeu: 7.16 ± 0.078	2.427GluMet: 2.427 ± 0.04	3.643GluAsn: 3.643 ± 0.058	2.122GluPro: 2.122 ± 0.04	3.658GluGln: 3.658 ± 0.056	3.847GluArg: 3.847 ± 0.056	3.285GluSer: 3.285 ± 0.051	4.009GluThr: 4.009 ± 0.055	4.953GluVal: 4.953 ± 0.061	0.925GluTrp: 0.925 ± 0.025	2.332GluTyr: 2.332 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	3.255PheAla: 3.255 ± 0.045	0.38PheCys: 0.38 ± 0.016	2.295PheAsp: 2.295 ± 0.039	2.569PheGlu: 2.569 ± 0.045	2.658PhePhe: 2.658 ± 0.05	3.2PheGly: 3.2 ± 0.05	1.237PheHis: 1.237 ± 0.027	3.78PheIle: 3.78 ± 0.063	1.903PheLys: 1.903 ± 0.036	4.668PheLeu: 4.668 ± 0.059	1.169PheMet: 1.169 ± 0.027	1.627PheAsn: 1.627 ± 0.031	1.755PhePro: 1.755 ± 0.04	1.901PheGln: 1.901 ± 0.034	1.846PheArg: 1.846 ± 0.032	3.417PheSer: 3.417 ± 0.048	2.676PheThr: 2.676 ± 0.051	3.207PheVal: 3.207 ± 0.049	0.491PheTrp: 0.491 ± 0.019	1.73PheTyr: 1.73 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	5.048GlyAla: 5.048 ± 0.063	0.672GlyCys: 0.672 ± 0.023	3.334GlyAsp: 3.334 ± 0.048	5.004GlyGlu: 5.004 ± 0.066	3.277GlyPhe: 3.277 ± 0.046	4.989GlyGly: 4.989 ± 0.068	1.335GlyHis: 1.335 ± 0.03	5.738GlyIle: 5.738 ± 0.065	4.841GlyLys: 4.841 ± 0.062	6.252GlyLeu: 6.252 ± 0.064	2.2GlyMet: 2.2 ± 0.039	2.813GlyAsn: 2.813 ± 0.051	1.699GlyPro: 1.699 ± 0.038	2.22GlyGln: 2.22 ± 0.045	2.939GlyArg: 2.939 ± 0.052	3.992GlySer: 3.992 ± 0.044	3.974GlyThr: 3.974 ± 0.057	4.908GlyVal: 4.908 ± 0.061	0.911GlyTrp: 0.911 ± 0.027	2.833GlyTyr: 2.833 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	1.459HisAla: 1.459 ± 0.035	0.251HisCys: 0.251 ± 0.013	1.12HisAsp: 1.12 ± 0.03	1.463HisGlu: 1.463 ± 0.034	1.204HisPhe: 1.204 ± 0.026	1.551HisGly: 1.551 ± 0.031	0.775HisHis: 0.775 ± 0.028	1.442HisIle: 1.442 ± 0.027	0.824HisLys: 0.824 ± 0.024	2.346HisLeu: 2.346 ± 0.048	0.479HisMet: 0.479 ± 0.017	0.641HisAsn: 0.641 ± 0.022	1.216HisPro: 1.216 ± 0.032	0.989HisGln: 0.989 ± 0.026	1.131HisArg: 1.131 ± 0.025	1.284HisSer: 1.284 ± 0.028	1.018HisThr: 1.018 ± 0.025	1.549HisVal: 1.549 ± 0.03	0.327HisTrp: 0.327 ± 0.015	0.914HisTyr: 0.914 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.05IleAla: 6.05 ± 0.067	0.642IleCys: 0.642 ± 0.021	4.239IleAsp: 4.239 ± 0.054	4.752IleGlu: 4.752 ± 0.059	3.256IlePhe: 3.256 ± 0.053	5.507IleGly: 5.507 ± 0.072	1.743IleHis: 1.743 ± 0.032	5.437IleIle: 5.437 ± 0.075	3.57IleLys: 3.57 ± 0.053	6.476IleLeu: 6.476 ± 0.083	1.622IleMet: 1.622 ± 0.036	2.986IleAsn: 2.986 ± 0.043	3.218IlePro: 3.218 ± 0.052	2.591IleGln: 2.591 ± 0.047	3.343IleArg: 3.343 ± 0.041	4.738IleSer: 4.738 ± 0.061	4.211IleThr: 4.211 ± 0.058	5.586IleVal: 5.586 ± 0.068	0.685IleTrp: 0.685 ± 0.023	2.445IleTyr: 2.445 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	4.637LysAla: 4.637 ± 0.061	0.334LysCys: 0.334 ± 0.017	2.934LysAsp: 2.934 ± 0.045	5.823LysGlu: 5.823 ± 0.069	1.486LysPhe: 1.486 ± 0.032	3.683LysGly: 3.683 ± 0.057	1.342LysHis: 1.342 ± 0.029	4.015LysIle: 4.015 ± 0.057	5.265LysLys: 5.265 ± 0.079	5.39LysLeu: 5.39 ± 0.064	1.855LysMet: 1.855 ± 0.036	3.028LysAsn: 3.028 ± 0.047	1.944LysPro: 1.944 ± 0.042	3.218LysGln: 3.218 ± 0.048	3.248LysArg: 3.248 ± 0.049	2.838LysSer: 2.838 ± 0.038	3.407LysThr: 3.407 ± 0.045	3.989LysVal: 3.989 ± 0.064	0.71LysTrp: 0.71 ± 0.019	2.077LysTyr: 2.077 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	7.856LeuAla: 7.856 ± 0.079	0.733LeuCys: 0.733 ± 0.021	4.723LeuAsp: 4.723 ± 0.064	6.743LeuGlu: 6.743 ± 0.076	5.055LeuPhe: 5.055 ± 0.079	5.932LeuGly: 5.932 ± 0.072	2.189LeuHis: 2.189 ± 0.039	6.665LeuIle: 6.665 ± 0.084	5.825LeuLys: 5.825 ± 0.071	10.908LeuLeu: 10.908 ± 0.118	2.532LeuMet: 2.532 ± 0.048	3.817LeuAsn: 3.817 ± 0.049	4.262LeuPro: 4.262 ± 0.057	3.89LeuGln: 3.89 ± 0.052	4.1LeuArg: 4.1 ± 0.055	6.606LeuSer: 6.606 ± 0.078	6.025LeuThr: 6.025 ± 0.062	6.051LeuVal: 6.051 ± 0.074	0.866LeuTrp: 0.866 ± 0.024	3.195LeuTyr: 3.195 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.031MetAla: 2.031 ± 0.034	0.152MetCys: 0.152 ± 0.01	1.387MetAsp: 1.387 ± 0.033	2.163MetGlu: 2.163 ± 0.036	1.132MetPhe: 1.132 ± 0.029	1.54MetGly: 1.54 ± 0.033	0.484MetHis: 0.484 ± 0.019	2.177MetIle: 2.177 ± 0.038	2.469MetLys: 2.469 ± 0.04	2.739MetLeu: 2.739 ± 0.048	0.969MetMet: 0.969 ± 0.026	1.526MetAsn: 1.526 ± 0.034	0.966MetPro: 0.966 ± 0.023	0.861MetGln: 0.861 ± 0.022	1.077MetArg: 1.077 ± 0.023	1.618MetSer: 1.618 ± 0.031	1.778MetThr: 1.778 ± 0.032	1.685MetVal: 1.685 ± 0.031	0.21MetTrp: 0.21 ± 0.011	0.818MetTyr: 0.818 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.694AsnAla: 2.694 ± 0.043	0.295AsnCys: 0.295 ± 0.012	2.358AsnAsp: 2.358 ± 0.04	3.257AsnGlu: 3.257 ± 0.043	1.421AsnPhe: 1.421 ± 0.026	3.345AsnGly: 3.345 ± 0.05	1.004AsnHis: 1.004 ± 0.025	3.267AsnIle: 3.267 ± 0.048	2.425AsnLys: 2.425 ± 0.039	3.162AsnLeu: 3.162 ± 0.05	1.059AsnMet: 1.059 ± 0.026	1.962AsnAsn: 1.962 ± 0.048	1.914AsnPro: 1.914 ± 0.04	1.682AsnGln: 1.682 ± 0.033	2.031AsnArg: 2.031 ± 0.038	2.166AsnSer: 2.166 ± 0.044	2.128AsnThr: 2.128 ± 0.029	2.889AsnVal: 2.889 ± 0.042	0.502AsnTrp: 0.502 ± 0.019	1.593AsnTyr: 1.593 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	2.947ProAla: 2.947 ± 0.042	0.247ProCys: 0.247 ± 0.013	2.291ProAsp: 2.291 ± 0.042	3.292ProGlu: 3.292 ± 0.055	2.032ProPhe: 2.032 ± 0.036	2.581ProGly: 2.581 ± 0.049	0.821ProHis: 0.821 ± 0.022	2.274ProIle: 2.274 ± 0.038	1.815ProLys: 1.815 ± 0.039	3.885ProLeu: 3.885 ± 0.055	0.857ProMet: 0.857 ± 0.025	1.374ProAsn: 1.374 ± 0.032	1.211ProPro: 1.211 ± 0.033	1.211ProGln: 1.211 ± 0.03	1.202ProArg: 1.202 ± 0.031	2.084ProSer: 2.084 ± 0.04	1.755ProThr: 1.755 ± 0.036	3.223ProVal: 3.223 ± 0.049	0.414ProTrp: 0.414 ± 0.017	1.459ProTyr: 1.459 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.071GlnAla: 3.071 ± 0.046	0.248GlnCys: 0.248 ± 0.013	1.491GlnAsp: 1.491 ± 0.032	2.959GlnGlu: 2.959 ± 0.044	1.607GlnPhe: 1.607 ± 0.034	2.182GlnGly: 2.182 ± 0.039	0.851GlnHis: 0.851 ± 0.024	2.44GlnIle: 2.44 ± 0.041	2.328GlnLys: 2.328 ± 0.038	4.69GlnLeu: 4.69 ± 0.058	1.186GlnMet: 1.186 ± 0.026	1.322GlnAsn: 1.322 ± 0.03	1.357GlnPro: 1.357 ± 0.035	2.276GlnGln: 2.276 ± 0.049	1.939GlnArg: 1.939 ± 0.042	2.115GlnSer: 2.115 ± 0.035	2.034GlnThr: 2.034 ± 0.041	2.394GlnVal: 2.394 ± 0.041	0.575GlnTrp: 0.575 ± 0.02	1.376GlnTyr: 1.376 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.669ArgAla: 2.669 ± 0.043	0.391ArgCys: 0.391 ± 0.019	2.041ArgAsp: 2.041 ± 0.037	3.577ArgGlu: 3.577 ± 0.057	2.209ArgPhe: 2.209 ± 0.045	2.67ArgGly: 2.67 ± 0.038	1.04ArgHis: 1.04 ± 0.027	3.169ArgIle: 3.169 ± 0.045	3.368ArgLys: 3.368 ± 0.052	4.679ArgLeu: 4.679 ± 0.063	1.443ArgMet: 1.443 ± 0.032	1.92ArgAsn: 1.92 ± 0.038	1.482ArgPro: 1.482 ± 0.032	2.036ArgGln: 2.036 ± 0.037	2.37ArgArg: 2.37 ± 0.044	2.529ArgSer: 2.529 ± 0.041	2.235ArgThr: 2.235 ± 0.039	2.646ArgVal: 2.646 ± 0.041	0.576ArgTrp: 0.576 ± 0.022	1.736ArgTyr: 1.736 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	4.177SerAla: 4.177 ± 0.056	0.475SerCys: 0.475 ± 0.018	2.925SerAsp: 2.925 ± 0.043	3.867SerGlu: 3.867 ± 0.067	3.267SerPhe: 3.267 ± 0.048	4.798SerGly: 4.798 ± 0.069	1.193SerHis: 1.193 ± 0.028	4.387SerIle: 4.387 ± 0.064	3.06SerLys: 3.06 ± 0.046	5.974SerLeu: 5.974 ± 0.06	1.673SerMet: 1.673 ± 0.034	2.147SerAsn: 2.147 ± 0.041	2.251SerPro: 2.251 ± 0.043	1.856SerGln: 1.856 ± 0.033	2.62SerArg: 2.62 ± 0.04	3.821SerSer: 3.821 ± 0.071	2.942SerThr: 2.942 ± 0.047	4.11SerVal: 4.11 ± 0.054	0.713SerTrp: 0.713 ± 0.023	2.195SerTyr: 2.195 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	4.826ThrAla: 4.826 ± 0.065	0.366ThrCys: 0.366 ± 0.014	2.788ThrAsp: 2.788 ± 0.048	3.652ThrGlu: 3.652 ± 0.046	2.581ThrPhe: 2.581 ± 0.043	4.298ThrGly: 4.298 ± 0.053	1.0ThrHis: 1.0 ± 0.027	4.359ThrIle: 4.359 ± 0.057	2.991ThrLys: 2.991 ± 0.042	5.124ThrLeu: 5.124 ± 0.058	1.474ThrMet: 1.474 ± 0.034	2.267ThrAsn: 2.267 ± 0.039	2.219ThrPro: 2.219 ± 0.04	1.302ThrGln: 1.302 ± 0.028	1.872ThrArg: 1.872 ± 0.035	3.108ThrSer: 3.108 ± 0.048	2.893ThrThr: 2.893 ± 0.046	4.535ThrVal: 4.535 ± 0.059	0.579ThrTrp: 0.579 ± 0.02	1.908ThrTyr: 1.908 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	5.196ValAla: 5.196 ± 0.064	0.619ValCys: 0.619 ± 0.021	3.711ValAsp: 3.711 ± 0.052	4.907ValGlu: 4.907 ± 0.062	3.475ValPhe: 3.475 ± 0.051	4.329ValGly: 4.329 ± 0.055	1.461ValHis: 1.461 ± 0.03	5.667ValIle: 5.667 ± 0.075	4.325ValLys: 4.325 ± 0.06	6.78ValLeu: 6.78 ± 0.073	1.886ValMet: 1.886 ± 0.033	3.108ValAsn: 3.108 ± 0.051	2.794ValPro: 2.794 ± 0.047	2.368ValGln: 2.368 ± 0.04	2.97ValArg: 2.97 ± 0.045	4.63ValSer: 4.63 ± 0.058	4.264ValThr: 4.264 ± 0.068	4.962ValVal: 4.962 ± 0.067	0.692ValTrp: 0.692 ± 0.023	2.512ValTyr: 2.512 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.638TrpAla: 0.638 ± 0.023	0.096TrpCys: 0.096 ± 0.008	0.54TrpAsp: 0.54 ± 0.022	0.798TrpGlu: 0.798 ± 0.024	0.538TrpPhe: 0.538 ± 0.02	0.75TrpGly: 0.75 ± 0.025	0.253TrpHis: 0.253 ± 0.013	0.815TrpIle: 0.815 ± 0.026	0.819TrpLys: 0.819 ± 0.023	1.364TrpLeu: 1.364 ± 0.03	0.384TrpMet: 0.384 ± 0.015	0.561TrpAsn: 0.561 ± 0.021	0.325TrpPro: 0.325 ± 0.014	0.506TrpGln: 0.506 ± 0.019	0.522TrpArg: 0.522 ± 0.019	0.641TrpSer: 0.641 ± 0.024	0.587TrpThr: 0.587 ± 0.021	0.622TrpVal: 0.622 ± 0.024	0.166TrpTrp: 0.166 ± 0.011	0.435TrpTyr: 0.435 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.234TyrAla: 2.234 ± 0.042	0.336TyrCys: 0.336 ± 0.016	1.96TyrAsp: 1.96 ± 0.039	2.54TyrGlu: 2.54 ± 0.044	1.961TyrPhe: 1.961 ± 0.035	2.622TyrGly: 2.622 ± 0.044	0.993TyrHis: 0.993 ± 0.024	2.435TyrIle: 2.435 ± 0.043	1.683TyrLys: 1.683 ± 0.036	3.662TyrLeu: 3.662 ± 0.051	0.849TyrMet: 0.849 ± 0.023	1.389TyrAsn: 1.389 ± 0.033	1.489TyrPro: 1.489 ± 0.038	1.615TyrGln: 1.615 ± 0.038	1.926TyrArg: 1.926 ± 0.033	2.251TyrSer: 2.251 ± 0.034	1.84TyrThr: 1.84 ± 0.039	2.29TyrVal: 2.29 ± 0.042	0.456TyrTrp: 0.456 ± 0.017	1.63TyrTyr: 1.63 ± 0.034	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5197 proteins (1567055 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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