Achromobacter phage vB_AxyP_19-32_Axy21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; unclassified Krylovirinae

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514CVU4|A0A514CVU4_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy21 OX=2591045 GN=Axy21_005 PE=4 SV=1
MM1 pKa = 7.97PDD3 pKa = 3.14DD4 pKa = 3.62TGAIIDD10 pKa = 3.53QTTFDD15 pKa = 3.64KK16 pKa = 11.03VQRR19 pKa = 11.84LAKK22 pKa = 10.0AARR25 pKa = 11.84LTEE28 pKa = 3.7QRR30 pKa = 11.84LRR32 pKa = 11.84YY33 pKa = 9.08YY34 pKa = 11.07ADD36 pKa = 3.5GQAQPGGNPGPYY48 pKa = 10.07LPLEE52 pKa = 4.28VDD54 pKa = 3.32QAVAEE59 pKa = 4.81LIAAAEE65 pKa = 4.17DD66 pKa = 3.52CGYY69 pKa = 9.05LTGAVEE75 pKa = 4.6PDD77 pKa = 3.46PSS79 pKa = 3.34

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514CVT2|A0A514CVT2_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy21 OX=2591045 GN=Axy21_003 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 9.3QTTIASAIQPGRR14 pKa = 11.84TSGAQALSVLKK25 pKa = 10.32QRR27 pKa = 11.84QLRR30 pKa = 11.84EE31 pKa = 3.5TGMTHH36 pKa = 5.06WQLLEE41 pKa = 4.12LYY43 pKa = 9.12RR44 pKa = 11.84TMLANKK50 pKa = 9.43AAGRR54 pKa = 11.84DD55 pKa = 3.67RR56 pKa = 11.84VWAVFYY62 pKa = 11.13DD63 pKa = 3.62NAQFPGRR70 pKa = 11.84PSRR73 pKa = 11.84VAGILTNWLRR83 pKa = 11.84CSVLVRR89 pKa = 11.84DD90 pKa = 5.3DD91 pKa = 4.28YY92 pKa = 12.11DD93 pKa = 3.63PAQCLWARR101 pKa = 11.84VIIVGMMM108 pKa = 3.59

Molecular weight: 12.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12915

55

1281

263.6

28.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.753 ± 0.533

0.697 ± 0.125

5.923 ± 0.158

5.412 ± 0.316

3.144 ± 0.192

7.743 ± 0.328

2.091 ± 0.197

4.135 ± 0.172

4.437 ± 0.234

8.502 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.131

3.569 ± 0.188

4.553 ± 0.34

5.01 ± 0.286

6.62 ± 0.314

5.513 ± 0.251

5.683 ± 0.338

7.046 ± 0.274

1.401 ± 0.139

2.942 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski