Psychrobacillus phage Perkons

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 217 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3YKS7|A0A6M3YKS7_9CAUD Uncharacterized protein OS=Psychrobacillus phage Perkons OX=2729574 PE=4 SV=1
MM1 pKa = 7.32QSPNTYY7 pKa = 9.8EE8 pKa = 4.25EE9 pKa = 4.53LVEE12 pKa = 4.14LCKK15 pKa = 10.81SLLNQGIDD23 pKa = 3.39EE24 pKa = 4.45NDD26 pKa = 3.25VYY28 pKa = 11.1EE29 pKa = 4.28QLDD32 pKa = 3.87GTIDD36 pKa = 3.42WSPDD40 pKa = 3.22SLHH43 pKa = 6.54YY44 pKa = 11.2ALDD47 pKa = 3.91DD48 pKa = 4.25AKK50 pKa = 11.17DD51 pKa = 3.49INGLLNQVKK60 pKa = 10.2

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3YLD6|A0A6M3YLD6_9CAUD Uncharacterized protein OS=Psychrobacillus phage Perkons OX=2729574 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.46LLRR5 pKa = 11.84VSGKK9 pKa = 10.26ALNSYY14 pKa = 9.74RR15 pKa = 11.84KK16 pKa = 9.08SVKK19 pKa = 10.36GNEE22 pKa = 4.09FTTKK26 pKa = 10.25DD27 pKa = 3.17QARR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 7.94LTRR35 pKa = 11.84NVALANEE42 pKa = 4.32VLHH45 pKa = 6.95KK46 pKa = 10.89SEE48 pKa = 4.01FQRR51 pKa = 11.84FIGTKK56 pKa = 9.32VYY58 pKa = 10.17TYY60 pKa = 10.95GNLHH64 pKa = 4.9ITVRR68 pKa = 11.84FGKK71 pKa = 9.98VVEE74 pKa = 4.44IVNHH78 pKa = 6.14SGGHH82 pKa = 6.35HH83 pKa = 5.19YY84 pKa = 10.68KK85 pKa = 10.03SWKK88 pKa = 10.01KK89 pKa = 10.83DD90 pKa = 3.14MKK92 pKa = 10.67KK93 pKa = 10.03YY94 pKa = 11.05AKK96 pKa = 8.87LTKK99 pKa = 9.86EE100 pKa = 4.59LNITDD105 pKa = 3.56

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

217

0

217

39968

29

2439

184.2

21.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.103 ± 0.284

0.918 ± 0.073

6.6 ± 0.102

8.384 ± 0.197

4.376 ± 0.143

5.297 ± 0.137

1.799 ± 0.099

8.212 ± 0.245

9.015 ± 0.213

8.577 ± 0.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.08

7.141 ± 0.136

1.949 ± 0.095

3.21 ± 0.168

3.505 ± 0.113

6.498 ± 0.221

5.655 ± 0.165

6.112 ± 0.176

1.098 ± 0.061

4.871 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski