Amino acid dipepetide frequency for uncultured phage_MedDCM-OCT-S46-C10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.762AlaAla: 3.762 ± 0.696
0.64AlaCys: 0.64 ± 0.207
4.483AlaAsp: 4.483 ± 0.546
4.323AlaGlu: 4.323 ± 0.803
2.882AlaPhe: 2.882 ± 0.372
3.522AlaGly: 3.522 ± 1.172
0.881AlaHis: 0.881 ± 0.263
5.443AlaIle: 5.443 ± 0.647
6.084AlaLys: 6.084 ± 0.692
4.723AlaLeu: 4.723 ± 0.542
2.161AlaMet: 2.161 ± 0.463
5.844AlaAsn: 5.844 ± 1.239
2.402AlaPro: 2.402 ± 0.409
2.882AlaGln: 2.882 ± 0.461
2.562AlaArg: 2.562 ± 0.492
6.004AlaSer: 6.004 ± 0.794
4.483AlaThr: 4.483 ± 0.803
4.483AlaVal: 4.483 ± 0.779
0.961AlaTrp: 0.961 ± 0.232
2.962AlaTyr: 2.962 ± 0.456
0.0AlaXaa: 0.0 ± 0.0
Cys
0.32CysAla: 0.32 ± 0.169
0.08CysCys: 0.08 ± 0.068
0.48CysAsp: 0.48 ± 0.186
0.24CysGlu: 0.24 ± 0.141
0.48CysPhe: 0.48 ± 0.21
0.72CysGly: 0.72 ± 0.298
0.32CysHis: 0.32 ± 0.143
0.56CysIle: 0.56 ± 0.261
1.281CysLys: 1.281 ± 0.275
0.961CysLeu: 0.961 ± 0.336
0.4CysMet: 0.4 ± 0.195
0.48CysAsn: 0.48 ± 0.325
0.32CysPro: 0.32 ± 0.152
0.4CysGln: 0.4 ± 0.154
0.56CysArg: 0.56 ± 0.211
0.881CysSer: 0.881 ± 0.257
0.56CysThr: 0.56 ± 0.165
0.64CysVal: 0.64 ± 0.222
0.0CysTrp: 0.0 ± 0.0
0.16CysTyr: 0.16 ± 0.113
0.0CysXaa: 0.0 ± 0.0
Asp
3.842AspAla: 3.842 ± 0.572
0.48AspCys: 0.48 ± 0.214
3.282AspAsp: 3.282 ± 0.719
4.083AspGlu: 4.083 ± 0.521
3.282AspPhe: 3.282 ± 0.432
2.562AspGly: 2.562 ± 0.438
0.56AspHis: 0.56 ± 0.242
3.282AspIle: 3.282 ± 0.349
5.043AspLys: 5.043 ± 0.71
6.004AspLeu: 6.004 ± 0.644
1.281AspMet: 1.281 ± 0.392
2.962AspAsn: 2.962 ± 0.438
1.841AspPro: 1.841 ± 0.379
1.041AspGln: 1.041 ± 0.311
2.001AspArg: 2.001 ± 0.292
4.403AspSer: 4.403 ± 0.624
4.003AspThr: 4.003 ± 0.578
4.803AspVal: 4.803 ± 0.67
0.961AspTrp: 0.961 ± 0.224
1.921AspTyr: 1.921 ± 0.308
0.0AspXaa: 0.0 ± 0.0
Glu
4.563GluAla: 4.563 ± 0.765
0.881GluCys: 0.881 ± 0.331
3.202GluAsp: 3.202 ± 0.484
4.403GluGlu: 4.403 ± 0.743
2.321GluPhe: 2.321 ± 0.395
3.762GluGly: 3.762 ± 0.448
1.361GluHis: 1.361 ± 0.323
4.643GluIle: 4.643 ± 0.589
5.524GluLys: 5.524 ± 0.939
6.004GluLeu: 6.004 ± 0.724
2.161GluMet: 2.161 ± 0.461
3.202GluAsn: 3.202 ± 0.498
1.361GluPro: 1.361 ± 0.473
3.042GluGln: 3.042 ± 0.652
3.202GluArg: 3.202 ± 0.509
3.762GluSer: 3.762 ± 0.533
4.643GluThr: 4.643 ± 0.592
2.802GluVal: 2.802 ± 0.534
1.041GluTrp: 1.041 ± 0.323
2.161GluTyr: 2.161 ± 0.474
0.0GluXaa: 0.0 ± 0.0
Phe
2.962PheAla: 2.962 ± 0.563
0.64PheCys: 0.64 ± 0.288
2.642PheAsp: 2.642 ± 0.695
2.001PheGlu: 2.001 ± 0.388
1.361PhePhe: 1.361 ± 0.29
2.402PheGly: 2.402 ± 0.465
0.32PheHis: 0.32 ± 0.137
2.241PheIle: 2.241 ± 0.406
3.762PheLys: 3.762 ± 0.573
3.442PheLeu: 3.442 ± 0.602
1.361PheMet: 1.361 ± 0.437
2.482PheAsn: 2.482 ± 0.351
0.64PhePro: 0.64 ± 0.197
1.521PheGln: 1.521 ± 0.232
1.281PheArg: 1.281 ± 0.26
2.802PheSer: 2.802 ± 0.467
2.962PheThr: 2.962 ± 0.578
2.482PheVal: 2.482 ± 0.382
0.48PheTrp: 0.48 ± 0.16
1.041PheTyr: 1.041 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
3.442GlyAla: 3.442 ± 0.643
0.48GlyCys: 0.48 ± 0.172
3.522GlyAsp: 3.522 ± 0.487
3.923GlyGlu: 3.923 ± 0.492
3.442GlyPhe: 3.442 ± 0.562
3.282GlyGly: 3.282 ± 0.597
1.201GlyHis: 1.201 ± 0.315
4.003GlyIle: 4.003 ± 0.599
5.123GlyLys: 5.123 ± 0.677
5.524GlyLeu: 5.524 ± 0.608
1.841GlyMet: 1.841 ± 0.313
4.083GlyAsn: 4.083 ± 0.684
0.32GlyPro: 0.32 ± 0.129
2.001GlyGln: 2.001 ± 0.369
2.321GlyArg: 2.321 ± 0.52
4.883GlySer: 4.883 ± 0.863
5.283GlyThr: 5.283 ± 1.046
4.483GlyVal: 4.483 ± 0.509
0.48GlyTrp: 0.48 ± 0.165
1.521GlyTyr: 1.521 ± 0.477
0.0GlyXaa: 0.0 ± 0.0
His
1.121HisAla: 1.121 ± 0.233
0.4HisCys: 0.4 ± 0.162
0.64HisAsp: 0.64 ± 0.186
0.4HisGlu: 0.4 ± 0.183
0.64HisPhe: 0.64 ± 0.179
1.041HisGly: 1.041 ± 0.251
0.56HisHis: 0.56 ± 0.221
0.801HisIle: 0.801 ± 0.218
0.881HisLys: 0.881 ± 0.289
1.841HisLeu: 1.841 ± 0.459
0.881HisMet: 0.881 ± 0.231
0.881HisAsn: 0.881 ± 0.233
0.64HisPro: 0.64 ± 0.203
0.4HisGln: 0.4 ± 0.206
0.4HisArg: 0.4 ± 0.16
0.961HisSer: 0.961 ± 0.241
1.521HisThr: 1.521 ± 0.38
1.201HisVal: 1.201 ± 0.301
0.48HisTrp: 0.48 ± 0.213
1.041HisTyr: 1.041 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
5.844IleAla: 5.844 ± 0.602
1.041IleCys: 1.041 ± 0.321
4.323IleAsp: 4.323 ± 0.668
5.123IleGlu: 5.123 ± 0.606
1.921IlePhe: 1.921 ± 0.34
3.602IleGly: 3.602 ± 0.612
0.64IleHis: 0.64 ± 0.178
4.883IleIle: 4.883 ± 0.695
6.404IleLys: 6.404 ± 0.711
4.483IleLeu: 4.483 ± 0.594
1.281IleMet: 1.281 ± 0.299
5.123IleAsn: 5.123 ± 0.614
2.321IlePro: 2.321 ± 0.557
2.081IleGln: 2.081 ± 0.431
2.402IleArg: 2.402 ± 0.398
4.323IleSer: 4.323 ± 0.665
5.123IleThr: 5.123 ± 0.998
2.962IleVal: 2.962 ± 0.416
0.64IleTrp: 0.64 ± 0.168
1.521IleTyr: 1.521 ± 0.4
0.0IleXaa: 0.0 ± 0.0
Lys
5.924LysAla: 5.924 ± 0.922
0.881LysCys: 0.881 ± 0.294
4.723LysAsp: 4.723 ± 0.633
5.524LysGlu: 5.524 ± 0.741
2.642LysPhe: 2.642 ± 0.464
4.243LysGly: 4.243 ± 0.629
1.841LysHis: 1.841 ± 0.444
6.004LysIle: 6.004 ± 0.739
7.125LysLys: 7.125 ± 1.179
6.964LysLeu: 6.964 ± 0.941
2.402LysMet: 2.402 ± 0.585
4.803LysAsn: 4.803 ± 0.839
3.522LysPro: 3.522 ± 0.447
2.882LysGln: 2.882 ± 0.538
4.643LysArg: 4.643 ± 0.727
4.083LysSer: 4.083 ± 0.464
5.363LysThr: 5.363 ± 0.599
5.844LysVal: 5.844 ± 0.662
0.801LysTrp: 0.801 ± 0.283
3.122LysTyr: 3.122 ± 0.598
0.0LysXaa: 0.0 ± 0.0
Leu
5.844LeuAla: 5.844 ± 0.671
0.64LeuCys: 0.64 ± 0.189
5.123LeuAsp: 5.123 ± 0.497
5.764LeuGlu: 5.764 ± 0.786
2.482LeuPhe: 2.482 ± 0.389
4.563LeuGly: 4.563 ± 0.503
1.121LeuHis: 1.121 ± 0.272
4.083LeuIle: 4.083 ± 0.598
8.325LeuLys: 8.325 ± 0.771
6.244LeuLeu: 6.244 ± 0.868
1.841LeuMet: 1.841 ± 0.346
5.363LeuAsn: 5.363 ± 0.539
3.362LeuPro: 3.362 ± 0.406
3.602LeuGln: 3.602 ± 0.599
3.602LeuArg: 3.602 ± 0.608
6.004LeuSer: 6.004 ± 0.691
6.164LeuThr: 6.164 ± 0.7
3.442LeuVal: 3.442 ± 0.54
0.961LeuTrp: 0.961 ± 0.226
2.962LeuTyr: 2.962 ± 0.465
0.0LeuXaa: 0.0 ± 0.0
Met
2.321MetAla: 2.321 ± 0.455
0.48MetCys: 0.48 ± 0.206
1.361MetAsp: 1.361 ± 0.303
0.961MetGlu: 0.961 ± 0.213
1.041MetPhe: 1.041 ± 0.305
1.601MetGly: 1.601 ± 0.354
0.24MetHis: 0.24 ± 0.1
1.521MetIle: 1.521 ± 0.361
1.761MetLys: 1.761 ± 0.403
1.761MetLeu: 1.761 ± 0.4
0.64MetMet: 0.64 ± 0.249
1.521MetAsn: 1.521 ± 0.413
1.041MetPro: 1.041 ± 0.234
0.64MetGln: 0.64 ± 0.309
1.441MetArg: 1.441 ± 0.347
2.642MetSer: 2.642 ± 0.416
1.521MetThr: 1.521 ± 0.355
1.121MetVal: 1.121 ± 0.255
0.56MetTrp: 0.56 ± 0.199
0.801MetTyr: 0.801 ± 0.21
0.0MetXaa: 0.0 ± 0.0
Asn
5.203AsnAla: 5.203 ± 0.637
0.48AsnCys: 0.48 ± 0.205
3.362AsnAsp: 3.362 ± 0.386
3.282AsnGlu: 3.282 ± 0.463
2.642AsnPhe: 2.642 ± 0.485
4.803AsnGly: 4.803 ± 0.604
0.72AsnHis: 0.72 ± 0.263
5.844AsnIle: 5.844 ± 0.877
5.524AsnLys: 5.524 ± 0.738
4.883AsnLeu: 4.883 ± 0.549
1.281AsnMet: 1.281 ± 0.395
4.083AsnAsn: 4.083 ± 0.594
1.921AsnPro: 1.921 ± 0.286
2.802AsnGln: 2.802 ± 0.437
2.562AsnArg: 2.562 ± 0.405
4.003AsnSer: 4.003 ± 0.638
4.163AsnThr: 4.163 ± 0.742
4.003AsnVal: 4.003 ± 0.93
0.56AsnTrp: 0.56 ± 0.247
1.761AsnTyr: 1.761 ± 0.329
0.0AsnXaa: 0.0 ± 0.0
Pro
1.361ProAla: 1.361 ± 0.337
0.4ProCys: 0.4 ± 0.167
1.601ProAsp: 1.601 ± 0.4
2.642ProGlu: 2.642 ± 0.609
1.761ProPhe: 1.761 ± 0.591
0.72ProGly: 0.72 ± 0.225
0.56ProHis: 0.56 ± 0.232
1.521ProIle: 1.521 ± 0.357
2.081ProLys: 2.081 ± 0.589
2.161ProLeu: 2.161 ± 0.372
0.48ProMet: 0.48 ± 0.222
2.001ProAsn: 2.001 ± 0.313
0.48ProPro: 0.48 ± 0.178
1.361ProGln: 1.361 ± 0.343
1.041ProArg: 1.041 ± 0.299
3.122ProSer: 3.122 ± 0.582
3.122ProThr: 3.122 ± 0.424
1.441ProVal: 1.441 ± 0.345
0.48ProTrp: 0.48 ± 0.226
1.281ProTyr: 1.281 ± 0.302
0.0ProXaa: 0.0 ± 0.0
Gln
2.241GlnAla: 2.241 ± 0.402
0.16GlnCys: 0.16 ± 0.108
2.241GlnAsp: 2.241 ± 0.556
3.602GlnGlu: 3.602 ± 0.583
1.441GlnPhe: 1.441 ± 0.283
2.161GlnGly: 2.161 ± 0.541
0.4GlnHis: 0.4 ± 0.148
2.802GlnIle: 2.802 ± 0.477
2.962GlnLys: 2.962 ± 0.512
3.442GlnLeu: 3.442 ± 0.497
0.881GlnMet: 0.881 ± 0.24
1.761GlnAsn: 1.761 ± 0.429
0.801GlnPro: 0.801 ± 0.269
1.601GlnGln: 1.601 ± 0.465
1.601GlnArg: 1.601 ± 0.384
2.241GlnSer: 2.241 ± 0.428
2.482GlnThr: 2.482 ± 0.4
2.802GlnVal: 2.802 ± 0.587
0.64GlnTrp: 0.64 ± 0.169
1.921GlnTyr: 1.921 ± 0.484
0.0GlnXaa: 0.0 ± 0.0
Arg
2.802ArgAla: 2.802 ± 0.451
0.08ArgCys: 0.08 ± 0.083
2.081ArgAsp: 2.081 ± 0.333
2.482ArgGlu: 2.482 ± 0.561
1.521ArgPhe: 1.521 ± 0.325
2.722ArgGly: 2.722 ± 0.549
1.201ArgHis: 1.201 ± 0.238
3.122ArgIle: 3.122 ± 0.685
2.882ArgLys: 2.882 ± 0.714
3.923ArgLeu: 3.923 ± 0.608
1.121ArgMet: 1.121 ± 0.263
2.402ArgAsn: 2.402 ± 0.416
1.041ArgPro: 1.041 ± 0.307
1.521ArgGln: 1.521 ± 0.302
2.241ArgArg: 2.241 ± 0.542
2.562ArgSer: 2.562 ± 0.397
2.001ArgThr: 2.001 ± 0.351
2.402ArgVal: 2.402 ± 0.448
0.881ArgTrp: 0.881 ± 0.299
1.921ArgTyr: 1.921 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
5.924SerAla: 5.924 ± 0.813
0.32SerCys: 0.32 ± 0.201
4.323SerAsp: 4.323 ± 0.596
5.203SerGlu: 5.203 ± 0.577
2.882SerPhe: 2.882 ± 0.49
5.684SerGly: 5.684 ± 0.767
1.281SerHis: 1.281 ± 0.296
4.483SerIle: 4.483 ± 0.548
5.443SerLys: 5.443 ± 0.749
5.043SerLeu: 5.043 ± 0.602
1.361SerMet: 1.361 ± 0.304
4.403SerAsn: 4.403 ± 0.76
1.521SerPro: 1.521 ± 0.319
3.362SerGln: 3.362 ± 0.508
2.482SerArg: 2.482 ± 0.463
5.684SerSer: 5.684 ± 1.096
6.004SerThr: 6.004 ± 1.037
4.163SerVal: 4.163 ± 0.842
0.881SerTrp: 0.881 ± 0.288
2.241SerTyr: 2.241 ± 0.493
0.0SerXaa: 0.0 ± 0.0
Thr
5.604ThrAla: 5.604 ± 0.758
0.56ThrCys: 0.56 ± 0.212
4.003ThrAsp: 4.003 ± 0.639
3.842ThrGlu: 3.842 ± 0.622
2.642ThrPhe: 2.642 ± 0.45
6.004ThrGly: 6.004 ± 0.739
1.041ThrHis: 1.041 ± 0.318
5.283ThrIle: 5.283 ± 0.714
5.443ThrLys: 5.443 ± 0.647
4.803ThrLeu: 4.803 ± 0.548
1.681ThrMet: 1.681 ± 0.418
5.043ThrAsn: 5.043 ± 0.855
2.482ThrPro: 2.482 ± 0.462
2.482ThrGln: 2.482 ± 0.443
2.161ThrArg: 2.161 ± 0.436
5.844ThrSer: 5.844 ± 1.213
5.764ThrThr: 5.764 ± 1.224
5.043ThrVal: 5.043 ± 1.272
0.56ThrTrp: 0.56 ± 0.206
2.161ThrTyr: 2.161 ± 0.492
0.0ThrXaa: 0.0 ± 0.0
Val
5.844ValAla: 5.844 ± 1.523
0.801ValCys: 0.801 ± 0.235
3.362ValAsp: 3.362 ± 0.441
3.602ValGlu: 3.602 ± 0.638
1.841ValPhe: 1.841 ± 0.367
4.323ValGly: 4.323 ± 0.474
1.361ValHis: 1.361 ± 0.359
3.122ValIle: 3.122 ± 0.463
3.602ValLys: 3.602 ± 0.611
5.203ValLeu: 5.203 ± 0.612
0.961ValMet: 0.961 ± 0.273
5.043ValAsn: 5.043 ± 1.013
2.321ValPro: 2.321 ± 0.459
3.042ValGln: 3.042 ± 0.557
2.962ValArg: 2.962 ± 0.521
4.323ValSer: 4.323 ± 0.702
4.243ValThr: 4.243 ± 0.635
3.442ValVal: 3.442 ± 0.493
0.48ValTrp: 0.48 ± 0.166
1.201ValTyr: 1.201 ± 0.264
0.0ValXaa: 0.0 ± 0.0
Trp
0.881TrpAla: 0.881 ± 0.28
0.08TrpCys: 0.08 ± 0.078
0.72TrpAsp: 0.72 ± 0.187
0.72TrpGlu: 0.72 ± 0.279
0.48TrpPhe: 0.48 ± 0.169
0.64TrpGly: 0.64 ± 0.175
0.24TrpHis: 0.24 ± 0.16
0.72TrpIle: 0.72 ± 0.223
1.201TrpLys: 1.201 ± 0.259
1.441TrpLeu: 1.441 ± 0.35
0.16TrpMet: 0.16 ± 0.107
0.56TrpAsn: 0.56 ± 0.27
0.08TrpPro: 0.08 ± 0.078
0.4TrpGln: 0.4 ± 0.184
0.32TrpArg: 0.32 ± 0.161
1.281TrpSer: 1.281 ± 0.504
0.801TrpThr: 0.801 ± 0.304
1.361TrpVal: 1.361 ± 0.328
0.24TrpTrp: 0.24 ± 0.132
0.24TrpTyr: 0.24 ± 0.119
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.681TyrAla: 1.681 ± 0.39
0.32TyrCys: 0.32 ± 0.149
2.241TyrAsp: 2.241 ± 0.285
1.921TyrGlu: 1.921 ± 0.437
1.121TyrPhe: 1.121 ± 0.286
2.802TyrGly: 2.802 ± 0.401
0.881TyrHis: 0.881 ± 0.25
1.681TyrIle: 1.681 ± 0.406
2.882TyrLys: 2.882 ± 0.418
2.642TyrLeu: 2.642 ± 0.349
0.72TyrMet: 0.72 ± 0.261
1.841TyrAsn: 1.841 ± 0.342
1.201TyrPro: 1.201 ± 0.252
1.121TyrGln: 1.121 ± 0.28
1.121TyrArg: 1.121 ± 0.335
2.882TyrSer: 2.882 ± 0.44
2.241TyrThr: 2.241 ± 0.391
2.161TyrVal: 2.161 ± 0.453
0.48TyrTrp: 0.48 ± 0.203
1.121TyrTyr: 1.121 ± 0.243
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (12493 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski