Pseudocohnilembus persalinus (Ciliate)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Oligohymenophorea; Scuticociliatia; Philasterida; Pseudocohnilembidae; Pseudocohnilembus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0V0QMI8|A0A0V0QMI8_PSEPJ Uncharacterized protein OS=Pseudocohnilembus persalinus OX=266149 GN=PPERSA_02823 PE=4 SV=1
MM1 pKa = 7.56FKK3 pKa = 10.82YY4 pKa = 10.7LLLAVLLISVTFAKK18 pKa = 10.75VSDD21 pKa = 3.44KK22 pKa = 11.52DD23 pKa = 3.52MEE25 pKa = 4.49NFTEE29 pKa = 5.06CMAEE33 pKa = 3.67IDD35 pKa = 4.08EE36 pKa = 4.82TCDD39 pKa = 3.3EE40 pKa = 4.43TVKK43 pKa = 11.02LSDD46 pKa = 3.77YY47 pKa = 10.9DD48 pKa = 3.6EE49 pKa = 5.35CKK51 pKa = 10.71ADD53 pKa = 4.88DD54 pKa = 4.32DD55 pKa = 4.66EE56 pKa = 7.04KK57 pKa = 11.23DD58 pKa = 3.59AEE60 pKa = 5.03DD61 pKa = 3.29ISGAEE66 pKa = 3.78FFGAIKK72 pKa = 10.4DD73 pKa = 4.04CYY75 pKa = 10.51GAVADD80 pKa = 6.06DD81 pKa = 5.49LDD83 pKa = 4.64GDD85 pKa = 4.2CKK87 pKa = 10.61KK88 pKa = 10.5YY89 pKa = 10.02YY90 pKa = 10.13QGIVDD95 pKa = 4.09CSGSVLGFALALLALVALLII115 pKa = 4.49

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0V0QD05|A0A0V0QD05_PSEPJ P-type ATPase cytoplasmic domain N OS=Pseudocohnilembus persalinus OX=266149 GN=PPERSA_05518 PE=4 SV=1
MM1 pKa = 6.75QAEE4 pKa = 4.34GFEE7 pKa = 4.21NEE9 pKa = 4.22RR10 pKa = 11.84RR11 pKa = 11.84PSSRR15 pKa = 11.84SPRR18 pKa = 11.84RR19 pKa = 11.84SDD21 pKa = 3.02EE22 pKa = 3.68RR23 pKa = 11.84DD24 pKa = 3.15RR25 pKa = 11.84RR26 pKa = 11.84DD27 pKa = 3.05SKK29 pKa = 10.7EE30 pKa = 3.45RR31 pKa = 11.84YY32 pKa = 9.36RR33 pKa = 11.84RR34 pKa = 11.84DD35 pKa = 2.79SYY37 pKa = 11.53RR38 pKa = 11.84DD39 pKa = 3.2DD40 pKa = 3.73RR41 pKa = 11.84DD42 pKa = 3.27RR43 pKa = 11.84RR44 pKa = 11.84DD45 pKa = 2.76SYY47 pKa = 11.49RR48 pKa = 11.84RR49 pKa = 11.84DD50 pKa = 3.07DD51 pKa = 3.56RR52 pKa = 11.84DD53 pKa = 2.81RR54 pKa = 11.84YY55 pKa = 10.57RR56 pKa = 11.84RR57 pKa = 11.84DD58 pKa = 3.06DD59 pKa = 3.66RR60 pKa = 11.84DD61 pKa = 3.22RR62 pKa = 11.84DD63 pKa = 3.71GYY65 pKa = 10.37RR66 pKa = 11.84GEE68 pKa = 3.97RR69 pKa = 11.84RR70 pKa = 11.84PYY72 pKa = 9.71DD73 pKa = 3.29DD74 pKa = 3.51RR75 pKa = 11.84RR76 pKa = 11.84PRR78 pKa = 11.84SSEE81 pKa = 3.85CYY83 pKa = 7.7NCGRR87 pKa = 11.84EE88 pKa = 3.91GHH90 pKa = 6.57IARR93 pKa = 11.84DD94 pKa = 3.73CKK96 pKa = 10.64EE97 pKa = 3.84PPKK100 pKa = 10.47RR101 pKa = 11.84RR102 pKa = 11.84DD103 pKa = 2.94MMRR106 pKa = 11.84RR107 pKa = 11.84DD108 pKa = 3.33SRR110 pKa = 11.84MGDD113 pKa = 3.03PRR115 pKa = 11.84GPRR118 pKa = 11.84RR119 pKa = 11.84PFICYY124 pKa = 7.78NCQGEE129 pKa = 4.25GHH131 pKa = 5.86MAKK134 pKa = 10.34DD135 pKa = 3.44CTKK138 pKa = 9.92PKK140 pKa = 10.16VEE142 pKa = 3.97RR143 pKa = 11.84RR144 pKa = 11.84MDD146 pKa = 3.16SRR148 pKa = 11.84RR149 pKa = 11.84DD150 pKa = 3.17DD151 pKa = 3.54RR152 pKa = 11.84YY153 pKa = 10.89RR154 pKa = 11.84RR155 pKa = 11.84DD156 pKa = 3.39DD157 pKa = 3.41RR158 pKa = 11.84GGRR161 pKa = 11.84RR162 pKa = 11.84DD163 pKa = 3.65DD164 pKa = 3.32RR165 pKa = 11.84DD166 pKa = 3.08RR167 pKa = 11.84RR168 pKa = 11.84YY169 pKa = 10.94DD170 pKa = 3.18PRR172 pKa = 11.84DD173 pKa = 3.07RR174 pKa = 11.84DD175 pKa = 3.12RR176 pKa = 11.84RR177 pKa = 11.84RR178 pKa = 11.84DD179 pKa = 3.73NKK181 pKa = 10.61CYY183 pKa = 10.29ACGEE187 pKa = 4.47DD188 pKa = 3.27GHH190 pKa = 6.31FARR193 pKa = 11.84DD194 pKa = 3.73CQSQDD199 pKa = 2.44KK200 pKa = 8.67TKK202 pKa = 10.35CYY204 pKa = 9.88KK205 pKa = 9.96CGKK208 pKa = 9.47VGHH211 pKa = 6.71LPKK214 pKa = 10.22NCRR217 pKa = 11.84RR218 pKa = 11.84RR219 pKa = 11.84SSSRR223 pKa = 11.84SSSSGSSSRR232 pKa = 11.84RR233 pKa = 11.84SSRR236 pKa = 11.84SGSSSSSTSRR246 pKa = 11.84SRR248 pKa = 11.84SRR250 pKa = 11.84SNKK253 pKa = 8.53RR254 pKa = 11.84GSRR257 pKa = 11.84SSSKK261 pKa = 10.77SSTGSKK267 pKa = 10.77SNN269 pKa = 3.34

Molecular weight: 32.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13175

0

13175

7254786

99

7962

550.6

64.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.867 ± 0.014

1.32 ± 0.02

5.328 ± 0.014

7.55 ± 0.026

4.852 ± 0.016

3.668 ± 0.018

1.448 ± 0.006

7.904 ± 0.018

10.061 ± 0.028

8.396 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.861 ± 0.008

9.756 ± 0.038

2.451 ± 0.011

10.892 ± 0.045

2.646 ± 0.013

6.645 ± 0.018

3.733 ± 0.016

3.75 ± 0.016

0.606 ± 0.005

4.263 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski