Amino acid dipepetide frequency for Bacillus phage vB_BtS_BMBtp14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.782AlaAla: 8.782 ± 2.657
0.66AlaCys: 0.66 ± 0.276
3.896AlaAsp: 3.896 ± 0.665
6.603AlaGlu: 6.603 ± 0.627
2.443AlaPhe: 2.443 ± 0.553
4.556AlaGly: 4.556 ± 1.151
1.585AlaHis: 1.585 ± 0.291
4.952AlaIle: 4.952 ± 0.589
6.471AlaLys: 6.471 ± 0.707
6.867AlaLeu: 6.867 ± 0.754
2.707AlaMet: 2.707 ± 0.55
3.104AlaAsn: 3.104 ± 0.761
1.849AlaPro: 1.849 ± 0.516
3.038AlaGln: 3.038 ± 0.791
2.971AlaArg: 2.971 ± 0.58
3.5AlaSer: 3.5 ± 0.575
3.764AlaThr: 3.764 ± 0.43
5.415AlaVal: 5.415 ± 0.608
0.858AlaTrp: 0.858 ± 0.256
0.924AlaTyr: 0.924 ± 0.296
0.0AlaXaa: 0.0 ± 0.0
Cys
0.264CysAla: 0.264 ± 0.137
0.198CysCys: 0.198 ± 0.116
0.33CysAsp: 0.33 ± 0.176
0.792CysGlu: 0.792 ± 0.312
0.264CysPhe: 0.264 ± 0.158
0.924CysGly: 0.924 ± 0.362
0.132CysHis: 0.132 ± 0.092
0.858CysIle: 0.858 ± 0.232
0.462CysLys: 0.462 ± 0.171
0.264CysLeu: 0.264 ± 0.121
0.198CysMet: 0.198 ± 0.111
0.528CysAsn: 0.528 ± 0.294
0.132CysPro: 0.132 ± 0.091
0.198CysGln: 0.198 ± 0.114
0.462CysArg: 0.462 ± 0.161
0.33CysSer: 0.33 ± 0.138
0.528CysThr: 0.528 ± 0.175
0.594CysVal: 0.594 ± 0.204
0.066CysTrp: 0.066 ± 0.053
0.462CysTyr: 0.462 ± 0.232
0.0CysXaa: 0.0 ± 0.0
Asp
4.424AspAla: 4.424 ± 0.718
0.264AspCys: 0.264 ± 0.128
2.773AspAsp: 2.773 ± 0.469
4.424AspGlu: 4.424 ± 0.479
2.707AspPhe: 2.707 ± 0.442
3.17AspGly: 3.17 ± 0.512
0.99AspHis: 0.99 ± 0.279
4.358AspIle: 4.358 ± 0.86
4.622AspLys: 4.622 ± 0.564
5.415AspLeu: 5.415 ± 0.444
1.585AspMet: 1.585 ± 0.304
2.245AspAsn: 2.245 ± 0.336
1.915AspPro: 1.915 ± 0.383
1.453AspGln: 1.453 ± 0.223
3.302AspArg: 3.302 ± 0.515
3.038AspSer: 3.038 ± 0.393
3.302AspThr: 3.302 ± 0.419
3.632AspVal: 3.632 ± 0.45
0.66AspTrp: 0.66 ± 0.302
2.575AspTyr: 2.575 ± 0.484
0.0AspXaa: 0.0 ± 0.0
Glu
6.801GluAla: 6.801 ± 0.52
0.462GluCys: 0.462 ± 0.156
5.018GluAsp: 5.018 ± 0.773
6.471GluGlu: 6.471 ± 0.989
3.104GluPhe: 3.104 ± 0.528
4.226GluGly: 4.226 ± 0.467
0.858GluHis: 0.858 ± 0.266
6.735GluIle: 6.735 ± 0.836
7.462GluLys: 7.462 ± 0.895
6.867GluLeu: 6.867 ± 0.906
2.047GluMet: 2.047 ± 0.35
4.688GluAsn: 4.688 ± 0.438
1.585GluPro: 1.585 ± 0.409
3.83GluGln: 3.83 ± 0.643
4.028GluArg: 4.028 ± 0.653
3.632GluSer: 3.632 ± 0.558
3.962GluThr: 3.962 ± 0.499
6.339GluVal: 6.339 ± 0.781
0.792GluTrp: 0.792 ± 0.234
2.707GluTyr: 2.707 ± 0.528
0.0GluXaa: 0.0 ± 0.0
Phe
2.707PheAla: 2.707 ± 0.549
0.264PheCys: 0.264 ± 0.153
2.179PheAsp: 2.179 ± 0.516
2.707PheGlu: 2.707 ± 0.447
1.321PhePhe: 1.321 ± 0.286
2.509PheGly: 2.509 ± 0.47
0.594PheHis: 0.594 ± 0.249
2.839PheIle: 2.839 ± 0.58
3.5PheLys: 3.5 ± 0.478
3.302PheLeu: 3.302 ± 0.602
1.585PheMet: 1.585 ± 0.327
2.047PheAsn: 2.047 ± 0.378
0.858PhePro: 0.858 ± 0.26
0.99PheGln: 0.99 ± 0.215
1.123PheArg: 1.123 ± 0.303
2.707PheSer: 2.707 ± 0.422
2.047PheThr: 2.047 ± 0.378
2.245PheVal: 2.245 ± 0.403
0.33PheTrp: 0.33 ± 0.16
1.255PheTyr: 1.255 ± 0.314
0.0PheXaa: 0.0 ± 0.0
Gly
4.358GlyAla: 4.358 ± 0.716
0.396GlyCys: 0.396 ± 0.171
2.707GlyAsp: 2.707 ± 0.511
3.896GlyGlu: 3.896 ± 0.439
3.038GlyPhe: 3.038 ± 0.597
3.83GlyGly: 3.83 ± 0.717
1.585GlyHis: 1.585 ± 0.329
5.151GlyIle: 5.151 ± 0.62
5.613GlyLys: 5.613 ± 0.602
3.566GlyLeu: 3.566 ± 0.639
1.915GlyMet: 1.915 ± 0.573
4.358GlyAsn: 4.358 ± 0.747
1.189GlyPro: 1.189 ± 0.378
1.849GlyGln: 1.849 ± 0.351
3.17GlyArg: 3.17 ± 0.478
3.962GlySer: 3.962 ± 0.602
3.698GlyThr: 3.698 ± 0.572
4.028GlyVal: 4.028 ± 0.476
1.123GlyTrp: 1.123 ± 0.275
1.783GlyTyr: 1.783 ± 0.398
0.0GlyXaa: 0.0 ± 0.0
His
0.858HisAla: 0.858 ± 0.252
0.132HisCys: 0.132 ± 0.12
0.726HisAsp: 0.726 ± 0.231
1.453HisGlu: 1.453 ± 0.358
0.99HisPhe: 0.99 ± 0.226
0.792HisGly: 0.792 ± 0.21
0.33HisHis: 0.33 ± 0.186
1.387HisIle: 1.387 ± 0.368
1.321HisLys: 1.321 ± 0.323
1.123HisLeu: 1.123 ± 0.25
0.33HisMet: 0.33 ± 0.112
0.792HisAsn: 0.792 ± 0.254
0.33HisPro: 0.33 ± 0.157
1.057HisGln: 1.057 ± 0.268
0.726HisArg: 0.726 ± 0.187
1.057HisSer: 1.057 ± 0.215
0.924HisThr: 0.924 ± 0.225
1.123HisVal: 1.123 ± 0.244
0.198HisTrp: 0.198 ± 0.114
0.726HisTyr: 0.726 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
5.877IleAla: 5.877 ± 0.723
0.726IleCys: 0.726 ± 0.252
5.217IleAsp: 5.217 ± 0.461
6.009IleGlu: 6.009 ± 0.763
1.849IlePhe: 1.849 ± 0.369
3.962IleGly: 3.962 ± 0.587
0.99IleHis: 0.99 ± 0.329
4.49IleIle: 4.49 ± 0.761
6.075IleLys: 6.075 ± 0.671
4.292IleLeu: 4.292 ± 0.494
1.783IleMet: 1.783 ± 0.297
4.49IleAsn: 4.49 ± 0.574
2.641IlePro: 2.641 ± 0.463
3.236IleGln: 3.236 ± 0.427
3.368IleArg: 3.368 ± 0.517
5.217IleSer: 5.217 ± 0.571
4.82IleThr: 4.82 ± 0.612
4.226IleVal: 4.226 ± 0.557
0.66IleTrp: 0.66 ± 0.212
1.717IleTyr: 1.717 ± 0.344
0.0IleXaa: 0.0 ± 0.0
Lys
6.207LysAla: 6.207 ± 1.014
0.726LysCys: 0.726 ± 0.288
4.754LysAsp: 4.754 ± 0.721
8.32LysGlu: 8.32 ± 0.922
2.113LysPhe: 2.113 ± 0.358
5.283LysGly: 5.283 ± 0.522
1.321LysHis: 1.321 ± 0.368
5.745LysIle: 5.745 ± 0.547
7.66LysLys: 7.66 ± 1.033
7.066LysLeu: 7.066 ± 0.707
2.443LysMet: 2.443 ± 0.454
3.764LysAsn: 3.764 ± 0.491
2.575LysPro: 2.575 ± 0.444
4.754LysGln: 4.754 ± 0.42
4.556LysArg: 4.556 ± 0.641
5.415LysSer: 5.415 ± 0.717
4.622LysThr: 4.622 ± 0.642
5.745LysVal: 5.745 ± 0.628
1.321LysTrp: 1.321 ± 0.304
3.302LysTyr: 3.302 ± 0.408
0.0LysXaa: 0.0 ± 0.0
Leu
5.283LeuAla: 5.283 ± 0.837
0.594LeuCys: 0.594 ± 0.191
5.085LeuAsp: 5.085 ± 0.671
6.339LeuGlu: 6.339 ± 0.642
3.038LeuPhe: 3.038 ± 0.481
4.688LeuGly: 4.688 ± 0.657
1.585LeuHis: 1.585 ± 0.402
4.754LeuIle: 4.754 ± 0.553
6.603LeuLys: 6.603 ± 0.545
6.867LeuLeu: 6.867 ± 1.206
2.113LeuMet: 2.113 ± 0.287
4.028LeuAsn: 4.028 ± 0.54
2.575LeuPro: 2.575 ± 0.473
5.151LeuGln: 5.151 ± 0.547
3.83LeuArg: 3.83 ± 0.452
4.622LeuSer: 4.622 ± 0.712
4.094LeuThr: 4.094 ± 0.483
4.16LeuVal: 4.16 ± 0.697
0.99LeuTrp: 0.99 ± 0.2
2.047LeuTyr: 2.047 ± 0.448
0.0LeuXaa: 0.0 ± 0.0
Met
1.981MetAla: 1.981 ± 0.425
0.33MetCys: 0.33 ± 0.149
1.651MetAsp: 1.651 ± 0.401
2.311MetGlu: 2.311 ± 0.368
0.858MetPhe: 0.858 ± 0.223
1.585MetGly: 1.585 ± 0.324
0.924MetHis: 0.924 ± 0.266
1.189MetIle: 1.189 ± 0.256
3.434MetLys: 3.434 ± 0.556
1.123MetLeu: 1.123 ± 0.279
0.396MetMet: 0.396 ± 0.258
1.849MetAsn: 1.849 ± 0.378
0.726MetPro: 0.726 ± 0.214
1.519MetGln: 1.519 ± 0.501
1.123MetArg: 1.123 ± 0.243
2.311MetSer: 2.311 ± 0.451
1.849MetThr: 1.849 ± 0.286
1.255MetVal: 1.255 ± 0.236
0.528MetTrp: 0.528 ± 0.181
0.792MetTyr: 0.792 ± 0.236
0.0MetXaa: 0.0 ± 0.0
Asn
4.556AsnAla: 4.556 ± 0.726
0.726AsnCys: 0.726 ± 0.199
2.773AsnAsp: 2.773 ± 0.407
3.632AsnGlu: 3.632 ± 0.505
1.651AsnPhe: 1.651 ± 0.288
3.896AsnGly: 3.896 ± 0.516
0.858AsnHis: 0.858 ± 0.232
3.566AsnIle: 3.566 ± 0.436
5.349AsnLys: 5.349 ± 0.497
3.962AsnLeu: 3.962 ± 0.559
1.255AsnMet: 1.255 ± 0.211
2.443AsnAsn: 2.443 ± 0.449
2.047AsnPro: 2.047 ± 0.37
1.717AsnGln: 1.717 ± 0.313
2.509AsnArg: 2.509 ± 0.406
2.707AsnSer: 2.707 ± 0.506
3.698AsnThr: 3.698 ± 0.509
3.17AsnVal: 3.17 ± 0.382
0.396AsnTrp: 0.396 ± 0.149
1.387AsnTyr: 1.387 ± 0.239
0.0AsnXaa: 0.0 ± 0.0
Pro
2.179ProAla: 2.179 ± 0.298
0.264ProCys: 0.264 ± 0.127
1.321ProAsp: 1.321 ± 0.352
2.311ProGlu: 2.311 ± 0.41
1.453ProPhe: 1.453 ± 0.278
2.047ProGly: 2.047 ± 0.312
0.66ProHis: 0.66 ± 0.185
2.113ProIle: 2.113 ± 0.445
2.377ProLys: 2.377 ± 0.405
2.311ProLeu: 2.311 ± 0.413
0.792ProMet: 0.792 ± 0.188
1.519ProAsn: 1.519 ± 0.287
1.123ProPro: 1.123 ± 0.348
0.858ProGln: 0.858 ± 0.267
1.585ProArg: 1.585 ± 0.32
1.585ProSer: 1.585 ± 0.288
1.849ProThr: 1.849 ± 0.32
2.245ProVal: 2.245 ± 0.481
0.132ProTrp: 0.132 ± 0.098
1.321ProTyr: 1.321 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
2.509GlnAla: 2.509 ± 0.413
0.198GlnCys: 0.198 ± 0.116
2.179GlnAsp: 2.179 ± 0.312
3.566GlnGlu: 3.566 ± 0.504
1.651GlnPhe: 1.651 ± 0.259
3.104GlnGly: 3.104 ± 0.564
0.396GlnHis: 0.396 ± 0.141
3.566GlnIle: 3.566 ± 0.509
3.368GlnLys: 3.368 ± 0.575
4.028GlnLeu: 4.028 ± 0.486
1.519GlnMet: 1.519 ± 0.323
1.717GlnAsn: 1.717 ± 0.312
1.255GlnPro: 1.255 ± 0.231
2.245GlnGln: 2.245 ± 0.497
2.377GlnArg: 2.377 ± 0.374
2.509GlnSer: 2.509 ± 0.351
2.707GlnThr: 2.707 ± 0.734
2.443GlnVal: 2.443 ± 0.396
0.33GlnTrp: 0.33 ± 0.143
1.519GlnTyr: 1.519 ± 0.289
0.0GlnXaa: 0.0 ± 0.0
Arg
2.047ArgAla: 2.047 ± 0.364
0.396ArgCys: 0.396 ± 0.204
2.311ArgAsp: 2.311 ± 0.463
3.632ArgGlu: 3.632 ± 0.5
1.915ArgPhe: 1.915 ± 0.462
2.575ArgGly: 2.575 ± 0.414
0.462ArgHis: 0.462 ± 0.175
4.424ArgIle: 4.424 ± 0.622
4.556ArgLys: 4.556 ± 0.608
3.83ArgLeu: 3.83 ± 0.423
1.453ArgMet: 1.453 ± 0.339
3.434ArgAsn: 3.434 ± 0.387
1.387ArgPro: 1.387 ± 0.316
1.717ArgGln: 1.717 ± 0.328
2.377ArgArg: 2.377 ± 0.33
2.971ArgSer: 2.971 ± 0.458
2.245ArgThr: 2.245 ± 0.409
3.5ArgVal: 3.5 ± 0.44
0.594ArgTrp: 0.594 ± 0.191
1.783ArgTyr: 1.783 ± 0.458
0.0ArgXaa: 0.0 ± 0.0
Ser
4.292SerAla: 4.292 ± 0.983
0.264SerCys: 0.264 ± 0.11
3.632SerAsp: 3.632 ± 0.393
4.82SerGlu: 4.82 ± 0.651
2.377SerPhe: 2.377 ± 0.44
3.896SerGly: 3.896 ± 0.536
0.99SerHis: 0.99 ± 0.24
5.018SerIle: 5.018 ± 0.566
4.358SerLys: 4.358 ± 0.61
4.424SerLeu: 4.424 ± 0.422
1.651SerMet: 1.651 ± 0.379
3.104SerAsn: 3.104 ± 0.535
2.377SerPro: 2.377 ± 0.419
2.311SerGln: 2.311 ± 0.521
2.377SerArg: 2.377 ± 0.396
3.038SerSer: 3.038 ± 0.52
3.302SerThr: 3.302 ± 0.406
3.698SerVal: 3.698 ± 0.374
0.792SerTrp: 0.792 ± 0.198
2.113SerTyr: 2.113 ± 0.375
0.0SerXaa: 0.0 ± 0.0
Thr
5.415ThrAla: 5.415 ± 0.829
0.528ThrCys: 0.528 ± 0.188
2.839ThrAsp: 2.839 ± 0.431
4.16ThrGlu: 4.16 ± 0.757
2.641ThrPhe: 2.641 ± 0.421
3.764ThrGly: 3.764 ± 0.5
0.792ThrHis: 0.792 ± 0.232
3.83ThrIle: 3.83 ± 0.498
4.952ThrLys: 4.952 ± 0.667
4.622ThrLeu: 4.622 ± 0.649
0.99ThrMet: 0.99 ± 0.25
2.839ThrAsn: 2.839 ± 0.524
2.113ThrPro: 2.113 ± 0.434
2.113ThrGln: 2.113 ± 0.547
1.717ThrArg: 1.717 ± 0.334
3.5ThrSer: 3.5 ± 0.561
3.434ThrThr: 3.434 ± 0.484
3.896ThrVal: 3.896 ± 0.534
0.594ThrTrp: 0.594 ± 0.197
1.981ThrTyr: 1.981 ± 0.407
0.0ThrXaa: 0.0 ± 0.0
Val
4.358ValAla: 4.358 ± 0.572
0.33ValCys: 0.33 ± 0.17
4.292ValAsp: 4.292 ± 0.604
6.141ValGlu: 6.141 ± 0.712
1.915ValPhe: 1.915 ± 0.392
3.896ValGly: 3.896 ± 0.552
0.528ValHis: 0.528 ± 0.165
3.962ValIle: 3.962 ± 0.565
5.679ValLys: 5.679 ± 0.565
4.688ValLeu: 4.688 ± 0.701
1.321ValMet: 1.321 ± 0.295
3.5ValAsn: 3.5 ± 0.423
2.509ValPro: 2.509 ± 0.486
3.368ValGln: 3.368 ± 0.392
3.302ValArg: 3.302 ± 0.443
4.424ValSer: 4.424 ± 0.563
3.83ValThr: 3.83 ± 0.548
4.16ValVal: 4.16 ± 0.549
0.594ValTrp: 0.594 ± 0.228
2.245ValTyr: 2.245 ± 0.46
0.0ValXaa: 0.0 ± 0.0
Trp
0.396TrpAla: 0.396 ± 0.125
0.264TrpCys: 0.264 ± 0.159
1.057TrpAsp: 1.057 ± 0.342
1.189TrpGlu: 1.189 ± 0.278
0.66TrpPhe: 0.66 ± 0.291
0.528TrpGly: 0.528 ± 0.18
0.264TrpHis: 0.264 ± 0.114
0.66TrpIle: 0.66 ± 0.227
1.057TrpLys: 1.057 ± 0.266
0.99TrpLeu: 0.99 ± 0.261
0.198TrpMet: 0.198 ± 0.121
0.264TrpAsn: 0.264 ± 0.143
0.066TrpPro: 0.066 ± 0.079
0.33TrpGln: 0.33 ± 0.159
0.594TrpArg: 0.594 ± 0.196
0.858TrpSer: 0.858 ± 0.234
0.396TrpThr: 0.396 ± 0.176
0.924TrpVal: 0.924 ± 0.229
0.264TrpTrp: 0.264 ± 0.142
0.66TrpTyr: 0.66 ± 0.213
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.651TyrAla: 1.651 ± 0.418
0.264TyrCys: 0.264 ± 0.127
2.113TyrAsp: 2.113 ± 0.413
2.839TyrGlu: 2.839 ± 0.445
1.123TyrPhe: 1.123 ± 0.265
1.981TyrGly: 1.981 ± 0.353
0.528TyrHis: 0.528 ± 0.197
2.047TyrIle: 2.047 ± 0.388
2.839TyrLys: 2.839 ± 0.356
2.839TyrLeu: 2.839 ± 0.509
1.387TyrMet: 1.387 ± 0.379
1.585TyrAsn: 1.585 ± 0.267
0.792TyrPro: 0.792 ± 0.273
1.453TyrGln: 1.453 ± 0.287
2.113TyrArg: 2.113 ± 0.466
1.453TyrSer: 1.453 ± 0.312
1.651TyrThr: 1.651 ± 0.32
2.245TyrVal: 2.245 ± 0.447
0.396TyrTrp: 0.396 ± 0.166
1.717TyrTyr: 1.717 ± 0.413
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (15145 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski