Amino acid dipepetide frequency for Clostridiales bacterium COT073_COT-073

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.382AlaAla: 6.382 ± 0.106	0.759AlaCys: 0.759 ± 0.029	4.374AlaAsp: 4.374 ± 0.062	6.349AlaGlu: 6.349 ± 0.082	3.252AlaPhe: 3.252 ± 0.062	5.755AlaGly: 5.755 ± 0.081	1.074AlaHis: 1.074 ± 0.034	5.702AlaIle: 5.702 ± 0.073	5.308AlaLys: 5.308 ± 0.095	7.029AlaLeu: 7.029 ± 0.103	2.198AlaMet: 2.198 ± 0.053	2.986AlaAsn: 2.986 ± 0.058	1.877AlaPro: 1.877 ± 0.053	2.363AlaGln: 2.363 ± 0.052	2.757AlaArg: 2.757 ± 0.056	3.247AlaSer: 3.247 ± 0.065	3.158AlaThr: 3.158 ± 0.066	5.341AlaVal: 5.341 ± 0.084	0.608AlaTrp: 0.608 ± 0.027	2.663AlaTyr: 2.663 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.602CysAla: 0.602 ± 0.027	0.165CysCys: 0.165 ± 0.013	0.537CysAsp: 0.537 ± 0.024	0.559CysGlu: 0.559 ± 0.025	0.529CysPhe: 0.529 ± 0.028	0.964CysGly: 0.964 ± 0.038	0.243CysHis: 0.243 ± 0.017	0.749CysIle: 0.749 ± 0.033	0.53CysLys: 0.53 ± 0.023	0.99CysLeu: 0.99 ± 0.031	0.292CysMet: 0.292 ± 0.018	0.402CysAsn: 0.402 ± 0.021	0.401CysPro: 0.401 ± 0.024	0.475CysGln: 0.475 ± 0.026	0.495CysArg: 0.495 ± 0.025	0.65CysSer: 0.65 ± 0.027	0.427CysThr: 0.427 ± 0.024	0.623CysVal: 0.623 ± 0.028	0.103CysTrp: 0.103 ± 0.01	0.439CysTyr: 0.439 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	2.955AspAla: 2.955 ± 0.062	0.576AspCys: 0.576 ± 0.025	2.317AspAsp: 2.317 ± 0.062	3.781AspGlu: 3.781 ± 0.069	3.01AspPhe: 3.01 ± 0.053	3.504AspGly: 3.504 ± 0.067	0.901AspHis: 0.901 ± 0.036	4.413AspIle: 4.413 ± 0.076	4.026AspLys: 4.026 ± 0.084	4.984AspLeu: 4.984 ± 0.074	1.468AspMet: 1.468 ± 0.042	2.266AspAsn: 2.266 ± 0.049	1.659AspPro: 1.659 ± 0.044	1.797AspGln: 1.797 ± 0.052	2.043AspArg: 2.043 ± 0.045	2.763AspSer: 2.763 ± 0.061	2.267AspThr: 2.267 ± 0.051	2.999AspVal: 2.999 ± 0.06	0.692AspTrp: 0.692 ± 0.023	2.649AspTyr: 2.649 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	4.984GluAla: 4.984 ± 0.07	0.577GluCys: 0.577 ± 0.025	3.644GluAsp: 3.644 ± 0.058	6.753GluGlu: 6.753 ± 0.116	2.796GluPhe: 2.796 ± 0.051	3.695GluGly: 3.695 ± 0.057	1.176GluHis: 1.176 ± 0.032	6.838GluIle: 6.838 ± 0.087	7.504GluLys: 7.504 ± 0.116	7.115GluLeu: 7.115 ± 0.098	2.54GluMet: 2.54 ± 0.059	4.709GluAsn: 4.709 ± 0.073	1.866GluPro: 1.866 ± 0.048	3.11GluGln: 3.11 ± 0.069	3.142GluArg: 3.142 ± 0.067	3.356GluSer: 3.356 ± 0.061	3.318GluThr: 3.318 ± 0.073	4.824GluVal: 4.824 ± 0.08	0.784GluTrp: 0.784 ± 0.028	3.193GluTyr: 3.193 ± 0.059	0.0GluXaa: 0.0 ± 0.0
Phe	3.298PheAla: 3.298 ± 0.061	0.603PheCys: 0.603 ± 0.024	2.498PheAsp: 2.498 ± 0.045	2.763PheGlu: 2.763 ± 0.05	2.234PhePhe: 2.234 ± 0.061	3.002PheGly: 3.002 ± 0.065	0.835PheHis: 0.835 ± 0.03	3.37PheIle: 3.37 ± 0.07	2.383PheLys: 2.383 ± 0.056	4.589PheLeu: 4.589 ± 0.083	1.185PheMet: 1.185 ± 0.038	1.758PheAsn: 1.758 ± 0.042	1.549PhePro: 1.549 ± 0.041	1.649PheGln: 1.649 ± 0.04	1.793PheArg: 1.793 ± 0.048	3.304PheSer: 3.304 ± 0.064	2.373PheThr: 2.373 ± 0.047	2.703PheVal: 2.703 ± 0.05	0.597PheTrp: 0.597 ± 0.024	2.145PheTyr: 2.145 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.341GlyAla: 4.341 ± 0.074	0.77GlyCys: 0.77 ± 0.03	2.931GlyAsp: 2.931 ± 0.055	4.419GlyGlu: 4.419 ± 0.072	3.159GlyPhe: 3.159 ± 0.066	4.296GlyGly: 4.296 ± 0.096	1.241GlyHis: 1.241 ± 0.041	6.023GlyIle: 6.023 ± 0.085	5.41GlyLys: 5.41 ± 0.077	6.228GlyLeu: 6.228 ± 0.084	2.138GlyMet: 2.138 ± 0.055	3.015GlyAsn: 3.015 ± 0.065	1.268GlyPro: 1.268 ± 0.04	2.721GlyGln: 2.721 ± 0.069	2.718GlyArg: 2.718 ± 0.063	3.71GlySer: 3.71 ± 0.068	3.194GlyThr: 3.194 ± 0.065	4.224GlyVal: 4.224 ± 0.064	0.654GlyTrp: 0.654 ± 0.027	3.264GlyTyr: 3.264 ± 0.08	0.0GlyXaa: 0.0 ± 0.0
His	0.923HisAla: 0.923 ± 0.032	0.222HisCys: 0.222 ± 0.016	0.857HisAsp: 0.857 ± 0.028	0.953HisGlu: 0.953 ± 0.028	0.955HisPhe: 0.955 ± 0.031	1.116HisGly: 1.116 ± 0.039	0.468HisHis: 0.468 ± 0.028	1.34HisIle: 1.34 ± 0.039	1.035HisLys: 1.035 ± 0.033	1.798HisLeu: 1.798 ± 0.048	0.426HisMet: 0.426 ± 0.022	0.793HisAsn: 0.793 ± 0.031	0.793HisPro: 0.793 ± 0.032	0.917HisGln: 0.917 ± 0.033	0.793HisArg: 0.793 ± 0.03	1.097HisSer: 1.097 ± 0.034	0.789HisThr: 0.789 ± 0.031	0.821HisVal: 0.821 ± 0.034	0.208HisTrp: 0.208 ± 0.016	0.901HisTyr: 0.901 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.191IleAla: 6.191 ± 0.088	1.109IleCys: 1.109 ± 0.037	4.283IleAsp: 4.283 ± 0.065	5.368IleGlu: 5.368 ± 0.071	3.459IlePhe: 3.459 ± 0.07	5.482IleGly: 5.482 ± 0.084	1.387IleHis: 1.387 ± 0.034	6.088IleIle: 6.088 ± 0.113	5.283IleLys: 5.283 ± 0.075	8.277IleLeu: 8.277 ± 0.096	2.208IleMet: 2.208 ± 0.048	3.477IleAsn: 3.477 ± 0.064	3.242IlePro: 3.242 ± 0.065	2.545IleGln: 2.545 ± 0.051	3.571IleArg: 3.571 ± 0.072	5.659IleSer: 5.659 ± 0.08	4.357IleThr: 4.357 ± 0.068	4.635IleVal: 4.635 ± 0.08	0.792IleTrp: 0.792 ± 0.031	3.311IleTyr: 3.311 ± 0.061	0.0IleXaa: 0.0 ± 0.0
Lys	5.534LysAla: 5.534 ± 0.098	0.505LysCys: 0.505 ± 0.026	4.05LysAsp: 4.05 ± 0.075	7.337LysGlu: 7.337 ± 0.102	2.025LysPhe: 2.025 ± 0.051	4.283LysGly: 4.283 ± 0.078	1.09LysHis: 1.09 ± 0.035	6.127LysIle: 6.127 ± 0.079	6.497LysLys: 6.497 ± 0.097	6.12LysLeu: 6.12 ± 0.085	2.285LysMet: 2.285 ± 0.044	4.163LysAsn: 4.163 ± 0.081	2.283LysPro: 2.283 ± 0.056	3.061LysGln: 3.061 ± 0.063	2.906LysArg: 2.906 ± 0.063	3.717LysSer: 3.717 ± 0.057	3.991LysThr: 3.991 ± 0.07	4.396LysVal: 4.396 ± 0.076	0.765LysTrp: 0.765 ± 0.029	3.029LysTyr: 3.029 ± 0.064	0.0LysXaa: 0.0 ± 0.0
Leu	7.878LeuAla: 7.878 ± 0.097	1.103LeuCys: 1.103 ± 0.037	4.534LeuAsp: 4.534 ± 0.072	6.772LeuGlu: 6.772 ± 0.101	4.345LeuPhe: 4.345 ± 0.076	5.746LeuGly: 5.746 ± 0.081	1.526LeuHis: 1.526 ± 0.044	7.102LeuIle: 7.102 ± 0.105	7.225LeuLys: 7.225 ± 0.096	9.745LeuLeu: 9.745 ± 0.133	2.621LeuMet: 2.621 ± 0.06	4.522LeuAsn: 4.522 ± 0.08	4.016LeuPro: 4.016 ± 0.074	3.411LeuGln: 3.411 ± 0.064	3.788LeuArg: 3.788 ± 0.069	6.858LeuSer: 6.858 ± 0.091	5.27LeuThr: 5.27 ± 0.076	5.408LeuVal: 5.408 ± 0.089	0.951LeuTrp: 0.951 ± 0.034	3.474LeuTyr: 3.474 ± 0.066	0.0LeuXaa: 0.0 ± 0.0
Met	2.496MetAla: 2.496 ± 0.045	0.208MetCys: 0.208 ± 0.016	1.515MetAsp: 1.515 ± 0.043	2.228MetGlu: 2.228 ± 0.052	0.974MetPhe: 0.974 ± 0.034	1.815MetGly: 1.815 ± 0.053	0.385MetHis: 0.385 ± 0.019	2.525MetIle: 2.525 ± 0.064	2.469MetLys: 2.469 ± 0.054	2.579MetLeu: 2.579 ± 0.06	0.876MetMet: 0.876 ± 0.029	1.51MetAsn: 1.51 ± 0.04	1.192MetPro: 1.192 ± 0.037	1.143MetGln: 1.143 ± 0.049	1.028MetArg: 1.028 ± 0.034	1.562MetSer: 1.562 ± 0.035	1.642MetThr: 1.642 ± 0.048	1.826MetVal: 1.826 ± 0.04	0.206MetTrp: 0.206 ± 0.016	0.811MetTyr: 0.811 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.957AsnAla: 2.957 ± 0.06	0.453AsnCys: 0.453 ± 0.024	2.099AsnAsp: 2.099 ± 0.052	2.854AsnGlu: 2.854 ± 0.051	2.067AsnPhe: 2.067 ± 0.049	3.467AsnGly: 3.467 ± 0.077	1.024AsnHis: 1.024 ± 0.029	3.852AsnIle: 3.852 ± 0.062	3.142AsnLys: 3.142 ± 0.061	4.396AsnLeu: 4.396 ± 0.074	1.301AsnMet: 1.301 ± 0.034	2.049AsnAsn: 2.049 ± 0.051	2.201AsnPro: 2.201 ± 0.05	2.319AsnGln: 2.319 ± 0.058	2.06AsnArg: 2.06 ± 0.045	2.587AsnSer: 2.587 ± 0.052	2.283AsnThr: 2.283 ± 0.053	2.625AsnVal: 2.625 ± 0.053	0.575AsnTrp: 0.575 ± 0.029	2.297AsnTyr: 2.297 ± 0.049	0.0AsnXaa: 0.0 ± 0.0
Pro	2.711ProAla: 2.711 ± 0.06	0.279ProCys: 0.279 ± 0.018	2.032ProAsp: 2.032 ± 0.044	3.379ProGlu: 3.379 ± 0.06	1.641ProPhe: 1.641 ± 0.043	1.903ProGly: 1.903 ± 0.051	0.591ProHis: 0.591 ± 0.026	2.508ProIle: 2.508 ± 0.056	2.376ProLys: 2.376 ± 0.061	3.042ProLeu: 3.042 ± 0.058	0.886ProMet: 0.886 ± 0.033	1.583ProAsn: 1.583 ± 0.04	0.814ProPro: 0.814 ± 0.037	1.368ProGln: 1.368 ± 0.051	0.92ProArg: 0.92 ± 0.035	1.648ProSer: 1.648 ± 0.043	1.656ProThr: 1.656 ± 0.052	2.593ProVal: 2.593 ± 0.048	0.33ProTrp: 0.33 ± 0.02	1.466ProTyr: 1.466 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	3.188GlnAla: 3.188 ± 0.066	0.235GlnCys: 0.235 ± 0.016	1.778GlnAsp: 1.778 ± 0.042	3.051GlnGlu: 3.051 ± 0.058	1.41GlnPhe: 1.41 ± 0.043	2.263GlnGly: 2.263 ± 0.052	0.495GlnHis: 0.495 ± 0.022	3.477GlnIle: 3.477 ± 0.06	3.514GlnLys: 3.514 ± 0.066	3.387GlnLeu: 3.387 ± 0.057	1.366GlnMet: 1.366 ± 0.035	2.081GlnAsn: 2.081 ± 0.05	1.404GlnPro: 1.404 ± 0.058	1.495GlnGln: 1.495 ± 0.045	1.649GlnArg: 1.649 ± 0.041	2.117GlnSer: 2.117 ± 0.047	2.243GlnThr: 2.243 ± 0.056	2.437GlnVal: 2.437 ± 0.064	0.388GlnTrp: 0.388 ± 0.019	1.484GlnTyr: 1.484 ± 0.047	0.0GlnXaa: 0.0 ± 0.0
Arg	2.505ArgAla: 2.505 ± 0.051	0.389ArgCys: 0.389 ± 0.022	1.933ArgAsp: 1.933 ± 0.047	3.418ArgGlu: 3.418 ± 0.066	1.866ArgPhe: 1.866 ± 0.044	2.184ArgGly: 2.184 ± 0.053	0.786ArgHis: 0.786 ± 0.029	3.37ArgIle: 3.37 ± 0.061	3.229ArgLys: 3.229 ± 0.062	3.999ArgLeu: 3.999 ± 0.069	1.31ArgMet: 1.31 ± 0.041	1.961ArgAsn: 1.961 ± 0.047	1.268ArgPro: 1.268 ± 0.033	2.113ArgGln: 2.113 ± 0.054	1.931ArgArg: 1.931 ± 0.048	2.009ArgSer: 2.009 ± 0.049	1.785ArgThr: 1.785 ± 0.043	2.351ArgVal: 2.351 ± 0.05	0.386ArgTrp: 0.386 ± 0.02	1.803ArgTyr: 1.803 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	4.165SerAla: 4.165 ± 0.072	0.593SerCys: 0.593 ± 0.025	2.78SerAsp: 2.78 ± 0.057	3.981SerGlu: 3.981 ± 0.07	3.08SerPhe: 3.08 ± 0.054	4.856SerGly: 4.856 ± 0.069	1.041SerHis: 1.041 ± 0.038	4.299SerIle: 4.299 ± 0.069	3.758SerLys: 3.758 ± 0.078	5.559SerLeu: 5.559 ± 0.091	1.551SerMet: 1.551 ± 0.045	2.297SerAsn: 2.297 ± 0.055	1.908SerPro: 1.908 ± 0.046	2.259SerGln: 2.259 ± 0.054	2.408SerArg: 2.408 ± 0.053	3.401SerSer: 3.401 ± 0.071	2.583SerThr: 2.583 ± 0.056	3.69SerVal: 3.69 ± 0.066	0.593SerTrp: 0.593 ± 0.028	2.501SerTyr: 2.501 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	4.236ThrAla: 4.236 ± 0.071	0.391ThrCys: 0.391 ± 0.032	2.867ThrAsp: 2.867 ± 0.054	4.042ThrGlu: 4.042 ± 0.08	2.041ThrPhe: 2.041 ± 0.051	4.056ThrGly: 4.056 ± 0.072	0.79ThrHis: 0.79 ± 0.029	4.102ThrIle: 4.102 ± 0.079	3.113ThrLys: 3.113 ± 0.054	4.56ThrLeu: 4.56 ± 0.075	1.325ThrMet: 1.325 ± 0.039	2.109ThrAsn: 2.109 ± 0.058	1.92ThrPro: 1.92 ± 0.045	1.504ThrGln: 1.504 ± 0.043	1.627ThrArg: 1.627 ± 0.043	2.547ThrSer: 2.547 ± 0.058	2.457ThrThr: 2.457 ± 0.066	3.523ThrVal: 3.523 ± 0.072	0.454ThrTrp: 0.454 ± 0.024	1.84ThrTyr: 1.84 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	4.48ValAla: 4.48 ± 0.073	0.681ValCys: 0.681 ± 0.031	3.258ValAsp: 3.258 ± 0.059	4.374ValGlu: 4.374 ± 0.075	3.054ValPhe: 3.054 ± 0.063	3.907ValGly: 3.907 ± 0.076	0.879ValHis: 0.879 ± 0.03	5.218ValIle: 5.218 ± 0.075	4.444ValLys: 4.444 ± 0.079	6.328ValLeu: 6.328 ± 0.092	1.808ValMet: 1.808 ± 0.042	2.714ValAsn: 2.714 ± 0.051	2.181ValPro: 2.181 ± 0.05	1.881ValGln: 1.881 ± 0.045	2.487ValArg: 2.487 ± 0.054	4.06ValSer: 4.06 ± 0.067	3.217ValThr: 3.217 ± 0.074	4.344ValVal: 4.344 ± 0.075	0.551ValTrp: 0.551 ± 0.026	2.424ValTyr: 2.424 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.626TrpAla: 0.626 ± 0.028	0.092TrpCys: 0.092 ± 0.011	0.559TrpAsp: 0.559 ± 0.028	0.698TrpGlu: 0.698 ± 0.034	0.416TrpPhe: 0.416 ± 0.023	0.689TrpGly: 0.689 ± 0.029	0.225TrpHis: 0.225 ± 0.015	0.728TrpIle: 0.728 ± 0.031	0.754TrpLys: 0.754 ± 0.032	1.122TrpLeu: 1.122 ± 0.031	0.289TrpMet: 0.289 ± 0.018	0.564TrpAsn: 0.564 ± 0.028	0.252TrpPro: 0.252 ± 0.015	0.698TrpGln: 0.698 ± 0.029	0.437TrpArg: 0.437 ± 0.02	0.495TrpSer: 0.495 ± 0.024	0.455TrpThr: 0.455 ± 0.022	0.568TrpVal: 0.568 ± 0.022	0.143TrpTrp: 0.143 ± 0.012	0.403TrpTyr: 0.403 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.728TyrAla: 2.728 ± 0.066	0.465TyrCys: 0.465 ± 0.025	2.222TyrAsp: 2.222 ± 0.055	2.844TyrGlu: 2.844 ± 0.051	2.319TyrPhe: 2.319 ± 0.048	2.743TyrGly: 2.743 ± 0.064	1.047TyrHis: 1.047 ± 0.033	2.891TyrIle: 2.891 ± 0.058	2.162TyrLys: 2.162 ± 0.052	4.451TyrLeu: 4.451 ± 0.074	0.919TyrMet: 0.919 ± 0.033	1.65TyrAsn: 1.65 ± 0.045	1.635TyrPro: 1.635 ± 0.048	2.634TyrGln: 2.634 ± 0.056	2.046TyrArg: 2.046 ± 0.045	2.514TyrSer: 2.514 ± 0.054	2.037TyrThr: 2.037 ± 0.053	2.359TyrVal: 2.359 ± 0.054	0.43TyrTrp: 0.43 ± 0.024	2.124TyrTyr: 2.124 ± 0.059	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2881 proteins (986005 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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