Amino acid dipepetide frequency for Gordonia phage BetterKatz

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.99AlaAla: 20.99 ± 1.723
0.623AlaCys: 0.623 ± 0.188
9.53AlaAsp: 9.53 ± 0.942
7.35AlaGlu: 7.35 ± 0.934
3.426AlaPhe: 3.426 ± 0.563
14.326AlaGly: 14.326 ± 1.333
2.118AlaHis: 2.118 ± 0.341
4.671AlaIle: 4.671 ± 0.379
3.488AlaLys: 3.488 ± 0.538
9.903AlaLeu: 9.903 ± 0.741
2.055AlaMet: 2.055 ± 0.462
3.862AlaAsn: 3.862 ± 0.514
7.225AlaPro: 7.225 ± 1.135
3.675AlaGln: 3.675 ± 0.574
9.094AlaArg: 9.094 ± 1.021
6.166AlaSer: 6.166 ± 0.72
7.225AlaThr: 7.225 ± 0.761
9.654AlaVal: 9.654 ± 0.744
2.055AlaTrp: 2.055 ± 0.397
2.18AlaTyr: 2.18 ± 0.305
0.0AlaXaa: 0.0 ± 0.0
Cys
1.059CysAla: 1.059 ± 0.266
0.062CysCys: 0.062 ± 0.061
0.249CysAsp: 0.249 ± 0.129
0.747CysGlu: 0.747 ± 0.225
0.062CysPhe: 0.062 ± 0.064
1.557CysGly: 1.557 ± 0.386
0.125CysHis: 0.125 ± 0.092
0.249CysIle: 0.249 ± 0.127
0.187CysLys: 0.187 ± 0.109
0.623CysLeu: 0.623 ± 0.167
0.062CysMet: 0.062 ± 0.057
0.436CysAsn: 0.436 ± 0.228
0.623CysPro: 0.623 ± 0.219
0.062CysGln: 0.062 ± 0.049
0.685CysArg: 0.685 ± 0.232
0.374CysSer: 0.374 ± 0.13
0.81CysThr: 0.81 ± 0.21
0.436CysVal: 0.436 ± 0.187
0.249CysTrp: 0.249 ± 0.132
0.125CysTyr: 0.125 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
7.35AspAla: 7.35 ± 0.516
0.561AspCys: 0.561 ± 0.231
5.979AspAsp: 5.979 ± 0.812
3.924AspGlu: 3.924 ± 0.56
1.308AspPhe: 1.308 ± 0.302
7.973AspGly: 7.973 ± 0.76
1.619AspHis: 1.619 ± 0.307
2.616AspIle: 2.616 ± 0.424
2.055AspLys: 2.055 ± 0.364
6.229AspLeu: 6.229 ± 0.565
1.246AspMet: 1.246 ± 0.262
2.18AspAsn: 2.18 ± 0.355
4.36AspPro: 4.36 ± 0.457
3.239AspGln: 3.239 ± 0.375
5.107AspArg: 5.107 ± 0.791
3.177AspSer: 3.177 ± 0.451
4.547AspThr: 4.547 ± 0.645
5.419AspVal: 5.419 ± 0.612
1.682AspTrp: 1.682 ± 0.301
0.872AspTyr: 0.872 ± 0.219
0.0AspXaa: 0.0 ± 0.0
Glu
4.983GluAla: 4.983 ± 0.507
0.374GluCys: 0.374 ± 0.134
2.741GluAsp: 2.741 ± 0.5
2.055GluGlu: 2.055 ± 0.493
0.997GluPhe: 0.997 ± 0.207
2.554GluGly: 2.554 ± 0.473
1.37GluHis: 1.37 ± 0.309
2.242GluIle: 2.242 ± 0.267
1.869GluLys: 1.869 ± 0.41
6.353GluLeu: 6.353 ± 0.488
1.246GluMet: 1.246 ± 0.364
2.305GluAsn: 2.305 ± 0.35
3.301GluPro: 3.301 ± 0.547
2.554GluGln: 2.554 ± 0.405
4.671GluArg: 4.671 ± 0.536
3.239GluSer: 3.239 ± 0.521
3.862GluThr: 3.862 ± 0.546
3.613GluVal: 3.613 ± 0.649
1.495GluTrp: 1.495 ± 0.293
1.308GluTyr: 1.308 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
4.36PheAla: 4.36 ± 0.497
0.125PheCys: 0.125 ± 0.079
2.118PheAsp: 2.118 ± 0.271
1.931PheGlu: 1.931 ± 0.259
0.436PhePhe: 0.436 ± 0.138
2.055PheGly: 2.055 ± 0.35
0.249PheHis: 0.249 ± 0.146
0.934PheIle: 0.934 ± 0.285
0.81PheLys: 0.81 ± 0.26
1.121PheLeu: 1.121 ± 0.24
0.498PheMet: 0.498 ± 0.143
0.498PheAsn: 0.498 ± 0.2
1.059PhePro: 1.059 ± 0.28
0.498PheGln: 0.498 ± 0.189
1.993PheArg: 1.993 ± 0.304
1.993PheSer: 1.993 ± 0.375
1.806PheThr: 1.806 ± 0.295
2.305PheVal: 2.305 ± 0.389
0.374PheTrp: 0.374 ± 0.155
0.498PheTyr: 0.498 ± 0.143
0.0PheXaa: 0.0 ± 0.0
Gly
10.9GlyAla: 10.9 ± 1.043
0.934GlyCys: 0.934 ± 0.305
7.225GlyAsp: 7.225 ± 0.791
3.488GlyGlu: 3.488 ± 0.541
2.865GlyPhe: 2.865 ± 0.409
9.53GlyGly: 9.53 ± 1.509
2.803GlyHis: 2.803 ± 0.504
5.232GlyIle: 5.232 ± 0.877
3.052GlyLys: 3.052 ± 0.37
7.225GlyLeu: 7.225 ± 0.961
1.121GlyMet: 1.121 ± 0.301
1.993GlyAsn: 1.993 ± 0.379
3.986GlyPro: 3.986 ± 0.498
2.927GlyGln: 2.927 ± 0.423
7.225GlyArg: 7.225 ± 0.653
6.042GlySer: 6.042 ± 0.721
6.727GlyThr: 6.727 ± 0.708
5.917GlyVal: 5.917 ± 0.794
1.433GlyTrp: 1.433 ± 0.317
2.118GlyTyr: 2.118 ± 0.38
0.0GlyXaa: 0.0 ± 0.0
His
2.741HisAla: 2.741 ± 0.466
0.125HisCys: 0.125 ± 0.086
1.931HisAsp: 1.931 ± 0.4
1.121HisGlu: 1.121 ± 0.277
0.498HisPhe: 0.498 ± 0.192
1.557HisGly: 1.557 ± 0.354
0.685HisHis: 0.685 ± 0.284
0.934HisIle: 0.934 ± 0.24
0.685HisLys: 0.685 ± 0.184
1.619HisLeu: 1.619 ± 0.319
0.311HisMet: 0.311 ± 0.143
0.561HisAsn: 0.561 ± 0.198
1.495HisPro: 1.495 ± 0.306
0.249HisGln: 0.249 ± 0.117
1.495HisArg: 1.495 ± 0.414
0.561HisSer: 0.561 ± 0.183
1.246HisThr: 1.246 ± 0.335
1.869HisVal: 1.869 ± 0.332
0.187HisTrp: 0.187 ± 0.1
0.311HisTyr: 0.311 ± 0.12
0.0HisXaa: 0.0 ± 0.0
Ile
6.478IleAla: 6.478 ± 0.564
0.249IleCys: 0.249 ± 0.121
3.862IleAsp: 3.862 ± 0.499
3.239IleGlu: 3.239 ± 0.38
0.561IlePhe: 0.561 ± 0.208
4.173IleGly: 4.173 ± 0.613
0.436IleHis: 0.436 ± 0.15
0.561IleIle: 0.561 ± 0.202
1.433IleLys: 1.433 ± 0.476
2.18IleLeu: 2.18 ± 0.263
0.436IleMet: 0.436 ± 0.138
0.81IleAsn: 0.81 ± 0.226
2.118IlePro: 2.118 ± 0.361
0.997IleGln: 0.997 ± 0.227
3.363IleArg: 3.363 ± 0.468
1.433IleSer: 1.433 ± 0.327
2.99IleThr: 2.99 ± 0.328
3.426IleVal: 3.426 ± 0.498
1.121IleTrp: 1.121 ± 0.312
0.436IleTyr: 0.436 ± 0.16
0.0IleXaa: 0.0 ± 0.0
Lys
3.488LysAla: 3.488 ± 0.572
0.311LysCys: 0.311 ± 0.136
1.183LysAsp: 1.183 ± 0.289
0.934LysGlu: 0.934 ± 0.243
0.872LysPhe: 0.872 ± 0.207
2.616LysGly: 2.616 ± 0.481
0.685LysHis: 0.685 ± 0.176
1.619LysIle: 1.619 ± 0.341
0.561LysLys: 0.561 ± 0.2
2.678LysLeu: 2.678 ± 0.543
0.934LysMet: 0.934 ± 0.212
0.872LysAsn: 0.872 ± 0.251
2.055LysPro: 2.055 ± 0.354
0.436LysGln: 0.436 ± 0.139
1.806LysArg: 1.806 ± 0.412
1.806LysSer: 1.806 ± 0.313
1.619LysThr: 1.619 ± 0.376
2.429LysVal: 2.429 ± 0.359
0.498LysTrp: 0.498 ± 0.17
0.623LysTyr: 0.623 ± 0.201
0.0LysXaa: 0.0 ± 0.0
Leu
9.841LeuAla: 9.841 ± 0.679
0.561LeuCys: 0.561 ± 0.208
5.294LeuAsp: 5.294 ± 0.603
5.668LeuGlu: 5.668 ± 0.597
2.429LeuPhe: 2.429 ± 0.304
6.976LeuGly: 6.976 ± 0.697
1.246LeuHis: 1.246 ± 0.23
3.301LeuIle: 3.301 ± 0.371
1.37LeuLys: 1.37 ± 0.314
6.291LeuLeu: 6.291 ± 0.647
1.682LeuMet: 1.682 ± 0.373
2.554LeuAsn: 2.554 ± 0.46
4.671LeuPro: 4.671 ± 0.592
2.491LeuGln: 2.491 ± 0.391
6.851LeuArg: 6.851 ± 0.844
4.485LeuSer: 4.485 ± 0.419
5.357LeuThr: 5.357 ± 0.606
5.917LeuVal: 5.917 ± 0.562
1.433LeuTrp: 1.433 ± 0.242
1.993LeuTyr: 1.993 ± 0.387
0.0LeuXaa: 0.0 ± 0.0
Met
2.678MetAla: 2.678 ± 0.31
0.125MetCys: 0.125 ± 0.092
0.249MetAsp: 0.249 ± 0.171
0.623MetGlu: 0.623 ± 0.191
0.997MetPhe: 0.997 ± 0.304
0.997MetGly: 0.997 ± 0.331
0.249MetHis: 0.249 ± 0.113
0.81MetIle: 0.81 ± 0.199
0.561MetLys: 0.561 ± 0.182
0.997MetLeu: 0.997 ± 0.247
0.374MetMet: 0.374 ± 0.135
0.498MetAsn: 0.498 ± 0.198
1.495MetPro: 1.495 ± 0.268
0.561MetGln: 0.561 ± 0.129
0.81MetArg: 0.81 ± 0.251
1.433MetSer: 1.433 ± 0.279
2.242MetThr: 2.242 ± 0.356
1.308MetVal: 1.308 ± 0.24
0.374MetTrp: 0.374 ± 0.128
0.125MetTyr: 0.125 ± 0.099
0.0MetXaa: 0.0 ± 0.0
Asn
3.239AsnAla: 3.239 ± 0.513
0.187AsnCys: 0.187 ± 0.09
1.682AsnAsp: 1.682 ± 0.332
1.183AsnGlu: 1.183 ± 0.318
0.872AsnPhe: 0.872 ± 0.208
3.239AsnGly: 3.239 ± 0.434
0.623AsnHis: 0.623 ± 0.215
0.997AsnIle: 0.997 ± 0.301
1.059AsnLys: 1.059 ± 0.222
2.554AsnLeu: 2.554 ± 0.53
0.498AsnMet: 0.498 ± 0.159
0.685AsnAsn: 0.685 ± 0.192
1.993AsnPro: 1.993 ± 0.3
0.934AsnGln: 0.934 ± 0.2
2.118AsnArg: 2.118 ± 0.363
0.997AsnSer: 0.997 ± 0.254
1.433AsnThr: 1.433 ± 0.255
1.744AsnVal: 1.744 ± 0.369
0.747AsnTrp: 0.747 ± 0.181
0.623AsnTyr: 0.623 ± 0.215
0.0AsnXaa: 0.0 ± 0.0
Pro
8.845ProAla: 8.845 ± 1.108
0.685ProCys: 0.685 ± 0.219
4.734ProAsp: 4.734 ± 0.806
2.865ProGlu: 2.865 ± 0.504
1.059ProPhe: 1.059 ± 0.214
5.294ProGly: 5.294 ± 0.633
1.37ProHis: 1.37 ± 0.288
2.055ProIle: 2.055 ± 0.383
1.993ProLys: 1.993 ± 0.387
3.239ProLeu: 3.239 ± 0.408
0.81ProMet: 0.81 ± 0.204
1.869ProAsn: 1.869 ± 0.287
2.865ProPro: 2.865 ± 0.541
2.118ProGln: 2.118 ± 0.438
3.301ProArg: 3.301 ± 0.451
2.554ProSer: 2.554 ± 0.258
5.045ProThr: 5.045 ± 0.527
4.547ProVal: 4.547 ± 0.456
1.121ProTrp: 1.121 ± 0.257
1.121ProTyr: 1.121 ± 0.316
0.0ProXaa: 0.0 ± 0.0
Gln
4.36GlnAla: 4.36 ± 0.632
0.187GlnCys: 0.187 ± 0.106
0.934GlnAsp: 0.934 ± 0.227
1.183GlnGlu: 1.183 ± 0.243
1.246GlnPhe: 1.246 ± 0.389
2.18GlnGly: 2.18 ± 0.428
0.81GlnHis: 0.81 ± 0.159
1.495GlnIle: 1.495 ± 0.263
0.872GlnLys: 0.872 ± 0.187
2.99GlnLeu: 2.99 ± 0.505
0.498GlnMet: 0.498 ± 0.17
0.81GlnAsn: 0.81 ± 0.21
2.242GlnPro: 2.242 ± 0.331
0.997GlnGln: 0.997 ± 0.281
1.993GlnArg: 1.993 ± 0.366
1.308GlnSer: 1.308 ± 0.382
1.495GlnThr: 1.495 ± 0.304
3.052GlnVal: 3.052 ± 0.459
0.997GlnTrp: 0.997 ± 0.314
0.374GlnTyr: 0.374 ± 0.149
0.0GlnXaa: 0.0 ± 0.0
Arg
9.779ArgAla: 9.779 ± 0.877
1.121ArgCys: 1.121 ± 0.297
5.855ArgAsp: 5.855 ± 0.635
4.422ArgGlu: 4.422 ± 0.469
2.18ArgPhe: 2.18 ± 0.508
5.73ArgGly: 5.73 ± 0.564
1.682ArgHis: 1.682 ± 0.407
4.049ArgIle: 4.049 ± 0.447
1.744ArgLys: 1.744 ± 0.332
6.54ArgLeu: 6.54 ± 0.708
1.495ArgMet: 1.495 ± 0.301
2.429ArgAsn: 2.429 ± 0.353
3.986ArgPro: 3.986 ± 0.598
1.682ArgGln: 1.682 ± 0.319
6.976ArgArg: 6.976 ± 0.808
2.803ArgSer: 2.803 ± 0.403
4.173ArgThr: 4.173 ± 0.442
5.73ArgVal: 5.73 ± 0.619
2.055ArgTrp: 2.055 ± 0.385
1.557ArgTyr: 1.557 ± 0.444
0.0ArgXaa: 0.0 ± 0.0
Ser
5.979SerAla: 5.979 ± 0.701
0.311SerCys: 0.311 ± 0.121
2.99SerAsp: 2.99 ± 0.471
2.242SerGlu: 2.242 ± 0.391
1.682SerPhe: 1.682 ± 0.354
6.042SerGly: 6.042 ± 0.634
1.121SerHis: 1.121 ± 0.204
1.806SerIle: 1.806 ± 0.296
1.806SerLys: 1.806 ± 0.385
4.485SerLeu: 4.485 ± 0.426
0.934SerMet: 0.934 ± 0.217
1.059SerAsn: 1.059 ± 0.227
2.803SerPro: 2.803 ± 0.469
1.744SerGln: 1.744 ± 0.306
3.363SerArg: 3.363 ± 0.394
4.235SerSer: 4.235 ± 0.973
4.734SerThr: 4.734 ± 0.522
3.924SerVal: 3.924 ± 0.477
0.934SerTrp: 0.934 ± 0.218
0.872SerTyr: 0.872 ± 0.214
0.0SerXaa: 0.0 ± 0.0
Thr
8.159ThrAla: 8.159 ± 0.657
0.997ThrCys: 0.997 ± 0.285
5.543ThrAsp: 5.543 ± 0.614
3.301ThrGlu: 3.301 ± 0.761
1.744ThrPhe: 1.744 ± 0.288
6.727ThrGly: 6.727 ± 0.745
1.246ThrHis: 1.246 ± 0.227
2.616ThrIle: 2.616 ± 0.408
2.242ThrLys: 2.242 ± 0.418
4.983ThrLeu: 4.983 ± 0.624
0.934ThrMet: 0.934 ± 0.211
1.121ThrAsn: 1.121 ± 0.206
3.924ThrPro: 3.924 ± 0.487
1.495ThrGln: 1.495 ± 0.353
5.294ThrArg: 5.294 ± 0.505
3.55ThrSer: 3.55 ± 0.595
5.793ThrThr: 5.793 ± 0.642
7.163ThrVal: 7.163 ± 0.522
0.934ThrTrp: 0.934 ± 0.289
1.557ThrTyr: 1.557 ± 0.295
0.0ThrXaa: 0.0 ± 0.0
Val
10.215ValAla: 10.215 ± 0.755
0.436ValCys: 0.436 ± 0.162
6.665ValAsp: 6.665 ± 0.653
5.668ValGlu: 5.668 ± 0.696
1.682ValPhe: 1.682 ± 0.364
6.104ValGly: 6.104 ± 0.685
0.997ValHis: 0.997 ± 0.272
2.616ValIle: 2.616 ± 0.395
1.37ValLys: 1.37 ± 0.272
6.727ValLeu: 6.727 ± 0.643
1.183ValMet: 1.183 ± 0.287
1.993ValAsn: 1.993 ± 0.356
5.17ValPro: 5.17 ± 0.526
2.055ValGln: 2.055 ± 0.366
5.979ValArg: 5.979 ± 0.587
4.235ValSer: 4.235 ± 0.532
5.668ValThr: 5.668 ± 0.612
7.661ValVal: 7.661 ± 0.759
1.744ValTrp: 1.744 ± 0.342
1.308ValTyr: 1.308 ± 0.275
0.0ValXaa: 0.0 ± 0.0
Trp
1.869TrpAla: 1.869 ± 0.315
0.561TrpCys: 0.561 ± 0.206
1.37TrpAsp: 1.37 ± 0.377
0.561TrpGlu: 0.561 ± 0.153
0.436TrpPhe: 0.436 ± 0.15
1.308TrpGly: 1.308 ± 0.281
0.436TrpHis: 0.436 ± 0.151
0.81TrpIle: 0.81 ± 0.213
0.498TrpLys: 0.498 ± 0.165
2.491TrpLeu: 2.491 ± 0.321
0.561TrpMet: 0.561 ± 0.162
0.561TrpAsn: 0.561 ± 0.16
1.37TrpPro: 1.37 ± 0.253
0.498TrpGln: 0.498 ± 0.186
1.37TrpArg: 1.37 ± 0.279
1.931TrpSer: 1.931 ± 0.411
0.934TrpThr: 0.934 ± 0.267
1.495TrpVal: 1.495 ± 0.397
0.498TrpTrp: 0.498 ± 0.202
0.81TrpTyr: 0.81 ± 0.26
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.305TyrAla: 2.305 ± 0.321
0.249TyrCys: 0.249 ± 0.11
1.37TyrAsp: 1.37 ± 0.323
0.623TyrGlu: 0.623 ± 0.169
0.311TyrPhe: 0.311 ± 0.138
1.806TyrGly: 1.806 ± 0.373
0.311TyrHis: 0.311 ± 0.154
0.685TyrIle: 0.685 ± 0.268
0.374TyrLys: 0.374 ± 0.137
1.183TyrLeu: 1.183 ± 0.256
0.561TyrMet: 0.561 ± 0.171
0.311TyrAsn: 0.311 ± 0.143
0.747TyrPro: 0.747 ± 0.25
0.81TyrGln: 0.81 ± 0.248
2.491TyrArg: 2.491 ± 0.484
0.747TyrSer: 0.747 ± 0.2
1.619TyrThr: 1.619 ± 0.399
1.869TyrVal: 1.869 ± 0.311
0.436TyrTrp: 0.436 ± 0.157
0.249TyrTyr: 0.249 ± 0.109
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (16056 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski