Streptococcus phage Javan247

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6A5Q5|A0A4D6A5Q5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan247 OX=2548069 GN=Javan247_0015 PE=4 SV=1
MM1 pKa = 8.0DD2 pKa = 4.56KK3 pKa = 11.2VKK5 pKa = 10.77LFQNEE10 pKa = 4.09VLGSGFDD17 pKa = 3.23IDD19 pKa = 6.24GYY21 pKa = 10.9FGWQCWDD28 pKa = 3.58GYY30 pKa = 11.61AKK32 pKa = 10.42YY33 pKa = 10.01CLWLGVPFSNCTDD46 pKa = 3.18SLYY49 pKa = 11.45VKK51 pKa = 10.14DD52 pKa = 4.36LWNQRR57 pKa = 11.84HH58 pKa = 5.51EE59 pKa = 4.37NGILDD64 pKa = 3.85YY65 pKa = 10.83FDD67 pKa = 4.26EE68 pKa = 4.56VEE70 pKa = 4.04NLEE73 pKa = 4.22GGEE76 pKa = 3.9VCIFVEE82 pKa = 4.97SPLTPVSHH90 pKa = 5.93VAIFVGDD97 pKa = 3.46VDD99 pKa = 4.08GVNGQFLGQNQGGAPGPFGGGAFDD123 pKa = 3.73VQIFPYY129 pKa = 10.3SILYY133 pKa = 6.8PTAFRR138 pKa = 11.84PKK140 pKa = 10.53GEE142 pKa = 4.23SLPKK146 pKa = 10.07QEE148 pKa = 4.58LKK150 pKa = 10.87EE151 pKa = 4.54AITEE155 pKa = 4.03VMDD158 pKa = 3.4NHH160 pKa = 6.12EE161 pKa = 4.51VPFFPEE167 pKa = 4.07DD168 pKa = 3.14ATFTVGDD175 pKa = 3.59SPINVRR181 pKa = 11.84RR182 pKa = 11.84EE183 pKa = 3.64PSLTGEE189 pKa = 4.08IVAVYY194 pKa = 10.01QPGDD198 pKa = 3.44KK199 pKa = 10.36VHH201 pKa = 6.55YY202 pKa = 9.59DD203 pKa = 3.47SKK205 pKa = 11.33GSNDD209 pKa = 3.13GYY211 pKa = 11.06RR212 pKa = 11.84WISYY216 pKa = 9.87VGEE219 pKa = 4.28SGNRR223 pKa = 11.84NYY225 pKa = 10.82LAIGQTDD232 pKa = 3.5EE233 pKa = 4.12AGNRR237 pKa = 11.84IDD239 pKa = 4.83LWGEE243 pKa = 3.77LSS245 pKa = 3.33

Molecular weight:
27.22 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D6A5V8|A0A4D6A5V8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan247 OX=2548069 GN=Javan247_0030 PE=4 SV=1
MM1 pKa = 7.73TGRR4 pKa = 11.84KK5 pKa = 9.63DD6 pKa = 3.38YY7 pKa = 10.83FMPFGVPSGFSFALEE22 pKa = 4.14HH23 pKa = 6.44GVKK26 pKa = 10.51LSNSAPRR33 pKa = 11.84GVKK36 pKa = 9.5QRR38 pKa = 11.84SS39 pKa = 3.13

Molecular weight:
4.29 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

10741

38

1268

228.5

25.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.448 ± 1.034

0.419 ± 0.087

6.424 ± 0.498

6.908 ± 0.583

3.957 ± 0.193

6.824 ± 0.49

1.322 ± 0.205

6.601 ± 0.259

8.156 ± 0.517

7.951 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.296

5.623 ± 0.371

3.203 ± 0.263

4.394 ± 0.278

3.947 ± 0.301

6.666 ± 0.609

5.642 ± 0.304

7.029 ± 0.432

1.015 ± 0.14

3.659 ± 0.435

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski