Microviridae sp. ctD0m35

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W9V5|A0A5Q2W9V5_9VIRU Putative minor capsid protein OS=Microviridae sp. ctD0m35 OX=2656694 PE=4 SV=1
MM1 pKa = 8.07PDD3 pKa = 3.24YY4 pKa = 11.15DD5 pKa = 3.62QLFPHH10 pKa = 7.12GSSDD14 pKa = 4.02YY15 pKa = 10.93DD16 pKa = 3.95GSWVLIKK23 pKa = 10.09MPWGCPP29 pKa = 3.22

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WAC6|A0A5Q2WAC6_9VIRU Replication associated protein OS=Microviridae sp. ctD0m35 OX=2656694 PE=4 SV=1
MM1 pKa = 7.09EE2 pKa = 5.62HH3 pKa = 6.97NYY5 pKa = 10.66DD6 pKa = 4.81AILNQMPFVGLFLGGILSAVAKK28 pKa = 9.55PIISVAAKK36 pKa = 10.18ALPFLKK42 pKa = 10.61NNAGSIINAGGNIAGGLIGAEE63 pKa = 4.09GAEE66 pKa = 4.58DD67 pKa = 3.9ANSANAMLARR77 pKa = 11.84EE78 pKa = 3.93NRR80 pKa = 11.84DD81 pKa = 2.87WQEE84 pKa = 3.8RR85 pKa = 11.84MSNTAHH91 pKa = 5.4QRR93 pKa = 11.84EE94 pKa = 4.51VEE96 pKa = 4.18DD97 pKa = 4.67LRR99 pKa = 11.84AAGINPILSAKK110 pKa = 10.17FGGSSTPPGNVATMTNPGEE129 pKa = 4.03FTGAGISSAGQAYY142 pKa = 6.65WTSQLTRR149 pKa = 11.84EE150 pKa = 4.11QVLTQRR156 pKa = 11.84SVQSVNSAQAMASRR170 pKa = 11.84AAALKK175 pKa = 10.73SLIDD179 pKa = 3.61AQKK182 pKa = 10.42IKK184 pKa = 11.27AEE186 pKa = 4.01IPAVQAQSEE195 pKa = 4.58RR196 pKa = 11.84EE197 pKa = 4.19SQGGDD202 pKa = 2.56IRR204 pKa = 11.84NSKK207 pKa = 9.58FFKK210 pKa = 8.49PFKK213 pKa = 9.79WFGEE217 pKa = 4.09IKK219 pKa = 9.67RR220 pKa = 11.84QIMGRR225 pKa = 3.56

Molecular weight: 23.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1435

29

528

205.0

22.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.129 ± 1.602

1.254 ± 0.557

5.784 ± 0.654

4.808 ± 0.31

4.181 ± 0.578

7.178 ± 0.579

2.021 ± 0.483

5.575 ± 0.653

5.645 ± 1.308

7.596 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.366

4.53 ± 0.635

6.132 ± 0.591

4.111 ± 0.631

5.645 ± 0.683

6.829 ± 0.669

5.993 ± 0.818

5.854 ± 0.561

1.254 ± 0.267

3.972 ± 0.752
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski