Streptococcus satellite phage Javan368

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLC7|A0A4D5ZLC7_9VIRU Putative transcriptional regulator OS=Streptococcus satellite phage Javan368 OX=2558664 GN=JavanS368_0016 PE=4 SV=1
MM1 pKa = 7.85SKK3 pKa = 10.08QLWNYY8 pKa = 9.17LRR10 pKa = 11.84SRR12 pKa = 11.84VQVVNSDD19 pKa = 3.73GEE21 pKa = 4.56VIKK24 pKa = 11.08GLVTDD29 pKa = 6.72FIDD32 pKa = 5.02EE33 pKa = 4.21MDD35 pKa = 4.15NDD37 pKa = 4.07EE38 pKa = 4.16QDD40 pKa = 4.11EE41 pKa = 4.31ITILIDD47 pKa = 3.58NPSPDD52 pKa = 3.9EE53 pKa = 3.93PTEE56 pKa = 3.76ISLFEE61 pKa = 4.13SEE63 pKa = 4.86IISIKK68 pKa = 10.53AISS71 pKa = 3.18

Molecular weight: 8.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZL39|A0A4D5ZL39_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan368 OX=2558664 GN=JavanS368_0009 PE=4 SV=1
MM1 pKa = 7.79NITEE5 pKa = 4.18YY6 pKa = 10.68KK7 pKa = 9.28KK8 pKa = 10.98KK9 pKa = 10.7NGATVYY15 pKa = 10.17RR16 pKa = 11.84ASVYY20 pKa = 10.83LGVDD24 pKa = 2.75KK25 pKa = 10.61LTGKK29 pKa = 10.25KK30 pKa = 10.31ARR32 pKa = 11.84TTVTASTKK40 pKa = 10.43KK41 pKa = 10.03GVKK44 pKa = 9.15IKK46 pKa = 10.77ARR48 pKa = 11.84EE49 pKa = 3.95AVNAFAANGYY59 pKa = 7.23SVKK62 pKa = 10.18EE63 pKa = 4.08KK64 pKa = 9.56PTITTYY70 pKa = 10.98RR71 pKa = 11.84EE72 pKa = 4.14LVALWWEE79 pKa = 4.57SYY81 pKa = 11.28KK82 pKa = 10.03NTIKK86 pKa = 10.78PNSQQSMEE94 pKa = 4.61GIVRR98 pKa = 11.84LHH100 pKa = 6.54ILPVFGDD107 pKa = 3.7YY108 pKa = 11.03KK109 pKa = 10.72LDD111 pKa = 3.64KK112 pKa = 9.61LTTPIIQQQVNKK124 pKa = 9.58WADD127 pKa = 3.34KK128 pKa = 10.76ANKK131 pKa = 9.24SEE133 pKa = 3.78KK134 pKa = 10.12GAYY137 pKa = 9.74ANYY140 pKa = 10.84SFLNNINRR148 pKa = 11.84RR149 pKa = 11.84ILQYY153 pKa = 10.72GVTMQVIQHH162 pKa = 5.79NPARR166 pKa = 11.84DD167 pKa = 3.51VIIPRR172 pKa = 11.84KK173 pKa = 7.62QQNKK177 pKa = 5.06EE178 pKa = 3.84HH179 pKa = 6.35KK180 pKa = 10.3VKK182 pKa = 10.6FFSNQEE188 pKa = 3.76LKK190 pKa = 10.86QFLDD194 pKa = 3.57YY195 pKa = 11.6LEE197 pKa = 6.02DD198 pKa = 4.68LDD200 pKa = 4.09QSSYY204 pKa = 11.42EE205 pKa = 3.92NFFDD209 pKa = 3.77YY210 pKa = 11.08VLYY213 pKa = 8.87KK214 pKa = 10.28TLLASGCRR222 pKa = 11.84IGEE225 pKa = 4.0ALALEE230 pKa = 4.49WSDD233 pKa = 3.65IDD235 pKa = 4.34LKK237 pKa = 11.13KK238 pKa = 10.51GTISISKK245 pKa = 8.13TLNRR249 pKa = 11.84YY250 pKa = 8.7QEE252 pKa = 4.35TNTPKK257 pKa = 10.57SKK259 pKa = 10.86AGLRR263 pKa = 11.84EE264 pKa = 3.44IDD266 pKa = 3.3IDD268 pKa = 3.65KK269 pKa = 9.94ATISLLKK276 pKa = 10.08QYY278 pKa = 10.82KK279 pKa = 9.6KK280 pKa = 10.25RR281 pKa = 11.84QQVQSWQLGRR291 pKa = 11.84SEE293 pKa = 5.54GIVFTPFTTKK303 pKa = 10.34YY304 pKa = 10.75AYY306 pKa = 10.29ACLLRR311 pKa = 11.84KK312 pKa = 9.67RR313 pKa = 11.84LQSHH317 pKa = 6.46FKK319 pKa = 10.39AAGVPDD325 pKa = 3.77ISFHH329 pKa = 5.86GFRR332 pKa = 11.84HH333 pKa = 4.4THH335 pKa = 4.65ATIMLYY341 pKa = 10.62AGIEE345 pKa = 4.18AKK347 pKa = 10.42DD348 pKa = 3.35LQYY351 pKa = 11.55RR352 pKa = 11.84LGHH355 pKa = 6.02SNISMTLNTYY365 pKa = 8.7VHH367 pKa = 6.14ATKK370 pKa = 10.47EE371 pKa = 4.25GAKK374 pKa = 9.7KK375 pKa = 10.24AVSIFEE381 pKa = 4.0AAISNLL387 pKa = 3.46

Molecular weight: 44.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

3422

37

573

190.1

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.458 ± 0.478

0.497 ± 0.116

5.903 ± 0.428

8.913 ± 0.725

3.711 ± 0.294

4.909 ± 0.371

1.549 ± 0.26

7.539 ± 0.454

8.475 ± 0.611

9.994 ± 0.452

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.016 ± 0.235

6.049 ± 0.367

2.659 ± 0.287

4.646 ± 0.454

4.734 ± 0.289

6.75 ± 0.544

5.143 ± 0.376

5.669 ± 0.458

1.023 ± 0.108

3.361 ± 0.399
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski