Amino acid dipepetide frequency for Escherichia virus P2_4E6b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.745AlaAla: 10.745 ± 1.903
0.723AlaCys: 0.723 ± 0.305
6.406AlaAsp: 6.406 ± 0.811
5.476AlaGlu: 5.476 ± 0.76
3.099AlaPhe: 3.099 ± 0.558
8.369AlaGly: 8.369 ± 0.94
1.653AlaHis: 1.653 ± 0.426
3.823AlaIle: 3.823 ± 0.627
5.269AlaLys: 5.269 ± 0.761
9.298AlaLeu: 9.298 ± 1.344
2.17AlaMet: 2.17 ± 0.423
2.686AlaAsn: 2.686 ± 0.6
4.443AlaPro: 4.443 ± 0.694
4.133AlaGln: 4.133 ± 0.661
5.063AlaArg: 5.063 ± 0.983
7.955AlaSer: 7.955 ± 0.803
6.509AlaThr: 6.509 ± 1.082
7.232AlaVal: 7.232 ± 0.879
1.55AlaTrp: 1.55 ± 0.417
2.583AlaTyr: 2.583 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
1.033CysAla: 1.033 ± 0.353
0.103CysCys: 0.103 ± 0.114
0.723CysAsp: 0.723 ± 0.285
0.207CysGlu: 0.207 ± 0.154
0.103CysPhe: 0.103 ± 0.104
0.517CysGly: 0.517 ± 0.22
0.103CysHis: 0.103 ± 0.103
0.517CysIle: 0.517 ± 0.213
0.207CysLys: 0.207 ± 0.154
0.62CysLeu: 0.62 ± 0.208
0.207CysMet: 0.207 ± 0.121
0.103CysAsn: 0.103 ± 0.092
0.413CysPro: 0.413 ± 0.199
0.827CysGln: 0.827 ± 0.312
0.93CysArg: 0.93 ± 0.286
0.517CysSer: 0.517 ± 0.202
0.723CysThr: 0.723 ± 0.349
0.62CysVal: 0.62 ± 0.223
0.103CysTrp: 0.103 ± 0.102
0.31CysTyr: 0.31 ± 0.176
0.0CysXaa: 0.0 ± 0.0
Asp
6.922AspAla: 6.922 ± 0.709
0.31AspCys: 0.31 ± 0.157
3.099AspAsp: 3.099 ± 0.5
3.719AspGlu: 3.719 ± 0.618
3.099AspPhe: 3.099 ± 0.649
5.682AspGly: 5.682 ± 0.722
0.62AspHis: 0.62 ± 0.246
4.133AspIle: 4.133 ± 0.743
2.48AspLys: 2.48 ± 0.467
4.236AspLeu: 4.236 ± 0.54
0.62AspMet: 0.62 ± 0.219
2.48AspAsn: 2.48 ± 0.606
1.653AspPro: 1.653 ± 0.395
1.446AspGln: 1.446 ± 0.437
1.963AspArg: 1.963 ± 0.519
2.686AspSer: 2.686 ± 0.456
3.513AspThr: 3.513 ± 0.527
3.823AspVal: 3.823 ± 0.534
1.136AspTrp: 1.136 ± 0.298
2.273AspTyr: 2.273 ± 0.461
0.0AspXaa: 0.0 ± 0.0
Glu
4.649GluAla: 4.649 ± 0.611
0.413GluCys: 0.413 ± 0.221
2.066GluAsp: 2.066 ± 0.479
3.513GluGlu: 3.513 ± 0.518
1.55GluPhe: 1.55 ± 0.405
2.48GluGly: 2.48 ± 0.519
2.066GluHis: 2.066 ± 0.489
2.686GluIle: 2.686 ± 0.474
4.133GluLys: 4.133 ± 0.584
7.542GluLeu: 7.542 ± 0.844
2.376GluMet: 2.376 ± 0.34
3.099GluAsn: 3.099 ± 0.715
2.79GluPro: 2.79 ± 0.577
2.893GluGln: 2.893 ± 0.662
5.166GluArg: 5.166 ± 0.907
4.029GluSer: 4.029 ± 0.703
3.409GluThr: 3.409 ± 0.664
3.719GluVal: 3.719 ± 0.56
1.136GluTrp: 1.136 ± 0.36
2.066GluTyr: 2.066 ± 0.39
0.0GluXaa: 0.0 ± 0.0
Phe
3.409PheAla: 3.409 ± 0.477
0.413PheCys: 0.413 ± 0.187
1.963PheAsp: 1.963 ± 0.375
1.963PheGlu: 1.963 ± 0.418
1.343PhePhe: 1.343 ± 0.312
1.136PheGly: 1.136 ± 0.334
0.723PheHis: 0.723 ± 0.296
1.653PheIle: 1.653 ± 0.399
2.376PheLys: 2.376 ± 0.408
3.409PheLeu: 3.409 ± 0.624
1.136PheMet: 1.136 ± 0.304
1.343PheAsn: 1.343 ± 0.353
1.033PhePro: 1.033 ± 0.313
1.136PheGln: 1.136 ± 0.302
1.653PheArg: 1.653 ± 0.334
2.583PheSer: 2.583 ± 0.583
3.203PheThr: 3.203 ± 0.462
1.756PheVal: 1.756 ± 0.529
0.827PheTrp: 0.827 ± 0.306
1.24PheTyr: 1.24 ± 0.382
0.0PheXaa: 0.0 ± 0.0
Gly
6.302GlyAla: 6.302 ± 1.073
0.827GlyCys: 0.827 ± 0.336
4.856GlyAsp: 4.856 ± 0.474
4.133GlyGlu: 4.133 ± 0.632
2.273GlyPhe: 2.273 ± 0.576
5.992GlyGly: 5.992 ± 0.985
0.723GlyHis: 0.723 ± 0.189
3.926GlyIle: 3.926 ± 0.609
5.476GlyLys: 5.476 ± 0.747
4.339GlyLeu: 4.339 ± 0.421
2.686GlyMet: 2.686 ± 0.592
2.893GlyAsn: 2.893 ± 0.861
0.93GlyPro: 0.93 ± 0.434
2.376GlyGln: 2.376 ± 0.464
5.063GlyArg: 5.063 ± 0.65
3.719GlySer: 3.719 ± 0.654
4.236GlyThr: 4.236 ± 0.756
5.682GlyVal: 5.682 ± 0.698
1.136GlyTrp: 1.136 ± 0.214
2.273GlyTyr: 2.273 ± 0.435
0.0GlyXaa: 0.0 ± 0.0
His
1.963HisAla: 1.963 ± 0.533
0.413HisCys: 0.413 ± 0.223
1.136HisAsp: 1.136 ± 0.345
1.24HisGlu: 1.24 ± 0.331
0.723HisPhe: 0.723 ± 0.323
1.653HisGly: 1.653 ± 0.503
0.827HisHis: 0.827 ± 0.236
1.24HisIle: 1.24 ± 0.279
0.62HisLys: 0.62 ± 0.229
1.86HisLeu: 1.86 ± 0.332
0.517HisMet: 0.517 ± 0.211
1.033HisAsn: 1.033 ± 0.393
1.136HisPro: 1.136 ± 0.311
1.136HisGln: 1.136 ± 0.344
1.136HisArg: 1.136 ± 0.354
0.62HisSer: 0.62 ± 0.285
0.93HisThr: 0.93 ± 0.304
0.93HisVal: 0.93 ± 0.267
0.31HisTrp: 0.31 ± 0.162
0.413HisTyr: 0.413 ± 0.178
0.0HisXaa: 0.0 ± 0.0
Ile
4.546IleAla: 4.546 ± 0.575
0.413IleCys: 0.413 ± 0.196
3.719IleAsp: 3.719 ± 0.656
3.306IleGlu: 3.306 ± 0.62
1.963IlePhe: 1.963 ± 0.476
4.236IleGly: 4.236 ± 0.661
0.31IleHis: 0.31 ± 0.146
2.996IleIle: 2.996 ± 0.464
2.376IleLys: 2.376 ± 0.621
3.099IleLeu: 3.099 ± 0.515
1.24IleMet: 1.24 ± 0.366
3.306IleAsn: 3.306 ± 0.53
1.963IlePro: 1.963 ± 0.498
1.86IleGln: 1.86 ± 0.402
4.649IleArg: 4.649 ± 0.633
4.236IleSer: 4.236 ± 0.65
4.856IleThr: 4.856 ± 0.62
3.099IleVal: 3.099 ± 0.556
0.62IleTrp: 0.62 ± 0.185
1.653IleTyr: 1.653 ± 0.317
0.0IleXaa: 0.0 ± 0.0
Lys
5.372LysAla: 5.372 ± 0.806
0.103LysCys: 0.103 ± 0.102
1.653LysAsp: 1.653 ± 0.477
2.996LysGlu: 2.996 ± 0.585
1.653LysPhe: 1.653 ± 0.479
2.893LysGly: 2.893 ± 0.403
1.446LysHis: 1.446 ± 0.433
2.273LysIle: 2.273 ± 0.443
3.409LysLys: 3.409 ± 0.636
6.096LysLeu: 6.096 ± 0.79
0.93LysMet: 0.93 ± 0.309
3.719LysAsn: 3.719 ± 0.818
2.996LysPro: 2.996 ± 0.55
1.756LysGln: 1.756 ± 0.43
3.926LysArg: 3.926 ± 0.727
3.203LysSer: 3.203 ± 0.587
4.236LysThr: 4.236 ± 0.526
3.513LysVal: 3.513 ± 0.505
0.827LysTrp: 0.827 ± 0.302
2.17LysTyr: 2.17 ± 0.477
0.0LysXaa: 0.0 ± 0.0
Leu
9.092LeuAla: 9.092 ± 0.96
0.413LeuCys: 0.413 ± 0.187
5.166LeuAsp: 5.166 ± 0.677
6.199LeuGlu: 6.199 ± 0.596
4.029LeuPhe: 4.029 ± 0.964
4.546LeuGly: 4.546 ± 0.721
1.86LeuHis: 1.86 ± 0.414
5.063LeuIle: 5.063 ± 0.579
5.372LeuLys: 5.372 ± 0.884
5.269LeuLeu: 5.269 ± 0.919
2.583LeuMet: 2.583 ± 0.616
4.959LeuAsn: 4.959 ± 0.513
4.029LeuPro: 4.029 ± 0.629
2.996LeuGln: 2.996 ± 0.646
4.753LeuArg: 4.753 ± 0.626
6.612LeuSer: 6.612 ± 0.892
7.129LeuThr: 7.129 ± 0.736
4.133LeuVal: 4.133 ± 0.636
0.93LeuTrp: 0.93 ± 0.246
2.48LeuTyr: 2.48 ± 0.541
0.0LeuXaa: 0.0 ± 0.0
Met
2.996MetAla: 2.996 ± 0.411
0.207MetCys: 0.207 ± 0.139
0.723MetAsp: 0.723 ± 0.277
1.343MetGlu: 1.343 ± 0.379
1.136MetPhe: 1.136 ± 0.351
0.93MetGly: 0.93 ± 0.301
0.62MetHis: 0.62 ± 0.22
1.033MetIle: 1.033 ± 0.353
1.446MetLys: 1.446 ± 0.333
2.686MetLeu: 2.686 ± 0.537
0.93MetMet: 0.93 ± 0.259
1.963MetAsn: 1.963 ± 0.512
1.343MetPro: 1.343 ± 0.373
0.723MetGln: 0.723 ± 0.232
2.17MetArg: 2.17 ± 0.457
1.86MetSer: 1.86 ± 0.449
2.893MetThr: 2.893 ± 0.527
1.446MetVal: 1.446 ± 0.36
0.207MetTrp: 0.207 ± 0.144
0.62MetTyr: 0.62 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
3.513AsnAla: 3.513 ± 0.582
0.413AsnCys: 0.413 ± 0.176
2.48AsnAsp: 2.48 ± 0.533
2.066AsnGlu: 2.066 ± 0.573
0.93AsnPhe: 0.93 ± 0.318
3.926AsnGly: 3.926 ± 0.834
0.62AsnHis: 0.62 ± 0.268
3.409AsnIle: 3.409 ± 0.568
2.376AsnLys: 2.376 ± 0.437
3.203AsnLeu: 3.203 ± 0.542
1.446AsnMet: 1.446 ± 0.33
1.963AsnAsn: 1.963 ± 0.379
1.963AsnPro: 1.963 ± 0.446
1.24AsnGln: 1.24 ± 0.306
2.79AsnArg: 2.79 ± 0.651
2.686AsnSer: 2.686 ± 0.64
1.756AsnThr: 1.756 ± 0.342
2.583AsnVal: 2.583 ± 0.528
0.413AsnTrp: 0.413 ± 0.17
1.343AsnTyr: 1.343 ± 0.436
0.0AsnXaa: 0.0 ± 0.0
Pro
4.236ProAla: 4.236 ± 0.732
0.103ProCys: 0.103 ± 0.11
3.513ProAsp: 3.513 ± 0.655
3.099ProGlu: 3.099 ± 0.527
1.24ProPhe: 1.24 ± 0.439
2.273ProGly: 2.273 ± 0.505
1.24ProHis: 1.24 ± 0.46
1.24ProIle: 1.24 ± 0.382
1.963ProLys: 1.963 ± 0.466
3.719ProLeu: 3.719 ± 0.56
0.93ProMet: 0.93 ± 0.308
0.517ProAsn: 0.517 ± 0.225
1.653ProPro: 1.653 ± 0.462
1.756ProGln: 1.756 ± 0.352
2.583ProArg: 2.583 ± 0.682
2.273ProSer: 2.273 ± 0.583
1.653ProThr: 1.653 ± 0.387
4.959ProVal: 4.959 ± 0.843
0.827ProTrp: 0.827 ± 0.244
0.723ProTyr: 0.723 ± 0.258
0.0ProXaa: 0.0 ± 0.0
Gln
4.339GlnAla: 4.339 ± 1.074
0.103GlnCys: 0.103 ± 0.1
2.48GlnAsp: 2.48 ± 0.509
2.273GlnGlu: 2.273 ± 0.566
0.723GlnPhe: 0.723 ± 0.238
1.756GlnGly: 1.756 ± 0.323
0.62GlnHis: 0.62 ± 0.252
2.273GlnIle: 2.273 ± 0.624
2.376GlnLys: 2.376 ± 0.474
3.616GlnLeu: 3.616 ± 0.682
0.827GlnMet: 0.827 ± 0.245
0.723GlnAsn: 0.723 ± 0.247
1.446GlnPro: 1.446 ± 0.404
2.066GlnGln: 2.066 ± 0.582
4.133GlnArg: 4.133 ± 0.685
2.48GlnSer: 2.48 ± 0.541
2.17GlnThr: 2.17 ± 0.404
2.066GlnVal: 2.066 ± 0.421
0.62GlnTrp: 0.62 ± 0.248
0.413GlnTyr: 0.413 ± 0.171
0.0GlnXaa: 0.0 ± 0.0
Arg
6.096ArgAla: 6.096 ± 0.736
1.033ArgCys: 1.033 ± 0.296
2.996ArgAsp: 2.996 ± 0.365
4.649ArgGlu: 4.649 ± 0.629
1.963ArgPhe: 1.963 ± 0.491
3.409ArgGly: 3.409 ± 0.649
1.756ArgHis: 1.756 ± 0.453
3.926ArgIle: 3.926 ± 0.577
3.513ArgLys: 3.513 ± 0.597
6.406ArgLeu: 6.406 ± 0.873
1.24ArgMet: 1.24 ± 0.36
2.376ArgAsn: 2.376 ± 0.476
2.48ArgPro: 2.48 ± 0.416
3.513ArgGln: 3.513 ± 0.816
5.786ArgArg: 5.786 ± 0.722
2.996ArgSer: 2.996 ± 0.656
3.306ArgThr: 3.306 ± 0.528
5.166ArgVal: 5.166 ± 0.901
1.24ArgTrp: 1.24 ± 0.302
3.203ArgTyr: 3.203 ± 0.581
0.0ArgXaa: 0.0 ± 0.0
Ser
6.612SerAla: 6.612 ± 0.957
0.62SerCys: 0.62 ± 0.238
3.513SerAsp: 3.513 ± 0.561
4.236SerGlu: 4.236 ± 0.67
1.86SerPhe: 1.86 ± 0.478
5.063SerGly: 5.063 ± 0.976
1.033SerHis: 1.033 ± 0.536
2.583SerIle: 2.583 ± 0.446
3.306SerLys: 3.306 ± 0.477
6.406SerLeu: 6.406 ± 1.118
1.963SerMet: 1.963 ± 0.382
2.376SerAsn: 2.376 ± 0.468
2.17SerPro: 2.17 ± 0.766
1.86SerGln: 1.86 ± 0.339
4.236SerArg: 4.236 ± 0.582
2.79SerSer: 2.79 ± 0.499
3.823SerThr: 3.823 ± 0.552
5.269SerVal: 5.269 ± 0.879
0.413SerTrp: 0.413 ± 0.151
1.55SerTyr: 1.55 ± 0.381
0.0SerXaa: 0.0 ± 0.0
Thr
7.232ThrAla: 7.232 ± 1.237
0.723ThrCys: 0.723 ± 0.318
3.926ThrAsp: 3.926 ± 0.557
3.306ThrGlu: 3.306 ± 0.642
2.376ThrPhe: 2.376 ± 0.407
7.335ThrGly: 7.335 ± 0.883
1.033ThrHis: 1.033 ± 0.287
3.719ThrIle: 3.719 ± 0.494
2.893ThrLys: 2.893 ± 0.626
7.026ThrLeu: 7.026 ± 0.918
1.86ThrMet: 1.86 ± 0.38
1.963ThrAsn: 1.963 ± 0.423
2.996ThrPro: 2.996 ± 0.402
1.963ThrGln: 1.963 ± 0.589
4.029ThrArg: 4.029 ± 0.492
3.099ThrSer: 3.099 ± 0.445
4.029ThrThr: 4.029 ± 0.857
4.236ThrVal: 4.236 ± 0.655
0.723ThrTrp: 0.723 ± 0.303
1.136ThrTyr: 1.136 ± 0.254
0.0ThrXaa: 0.0 ± 0.0
Val
5.992ValAla: 5.992 ± 0.844
1.446ValCys: 1.446 ± 0.386
3.513ValAsp: 3.513 ± 0.62
4.133ValGlu: 4.133 ± 0.765
2.48ValPhe: 2.48 ± 0.521
4.856ValGly: 4.856 ± 0.69
0.93ValHis: 0.93 ± 0.274
4.649ValIle: 4.649 ± 0.635
4.339ValLys: 4.339 ± 0.728
5.786ValLeu: 5.786 ± 0.837
2.066ValMet: 2.066 ± 0.377
2.066ValAsn: 2.066 ± 0.372
2.686ValPro: 2.686 ± 0.482
1.86ValGln: 1.86 ± 0.453
3.099ValArg: 3.099 ± 0.535
4.959ValSer: 4.959 ± 0.673
5.063ValThr: 5.063 ± 0.979
4.959ValVal: 4.959 ± 0.706
0.827ValTrp: 0.827 ± 0.386
1.55ValTyr: 1.55 ± 0.597
0.0ValXaa: 0.0 ± 0.0
Trp
1.033TrpAla: 1.033 ± 0.266
0.0TrpCys: 0.0 ± 0.0
1.033TrpAsp: 1.033 ± 0.31
1.24TrpGlu: 1.24 ± 0.273
0.517TrpPhe: 0.517 ± 0.202
0.723TrpGly: 0.723 ± 0.336
0.517TrpHis: 0.517 ± 0.252
0.827TrpIle: 0.827 ± 0.282
0.62TrpLys: 0.62 ± 0.271
1.446TrpLeu: 1.446 ± 0.438
0.413TrpMet: 0.413 ± 0.201
0.62TrpAsn: 0.62 ± 0.308
1.033TrpPro: 1.033 ± 0.333
0.517TrpGln: 0.517 ± 0.226
1.653TrpArg: 1.653 ± 0.516
0.723TrpSer: 0.723 ± 0.285
0.517TrpThr: 0.517 ± 0.225
0.413TrpVal: 0.413 ± 0.18
0.517TrpTrp: 0.517 ± 0.233
0.62TrpTyr: 0.62 ± 0.22
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.099TyrAla: 3.099 ± 0.59
0.103TyrCys: 0.103 ± 0.102
0.93TyrAsp: 0.93 ± 0.301
2.686TyrGlu: 2.686 ± 0.36
1.24TyrPhe: 1.24 ± 0.39
2.066TyrGly: 2.066 ± 0.47
1.136TyrHis: 1.136 ± 0.349
2.48TyrIle: 2.48 ± 0.453
0.62TyrLys: 0.62 ± 0.29
1.653TyrLeu: 1.653 ± 0.446
1.033TyrMet: 1.033 ± 0.284
0.93TyrAsn: 0.93 ± 0.315
1.343TyrPro: 1.343 ± 0.402
1.446TyrGln: 1.446 ± 0.428
2.273TyrArg: 2.273 ± 0.461
1.653TyrSer: 1.653 ± 0.356
1.653TyrThr: 1.653 ± 0.447
1.653TyrVal: 1.653 ± 0.397
0.62TyrTrp: 0.62 ± 0.238
1.446TyrTyr: 1.446 ± 0.348
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (9680 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski